Novel naphthylstyryl-pyridium potentiometric dyes offer advantages for neural network analysis.
AuthorsObaid AL, Loew LM, Wuskell JP, Salzberg BM
JournalJ Neurosci Methods
PubMed ID15003384
'The submucous plexus of the guinea pig intestine is a quasi-two-dimensional mammalian neural network that is particularly amenable to study using multiple site optical recording of transmembrane voltage (MSORTV) [Biol. Bull. 183 (1992) 344; J. Neurosci. 19 (1999) 3073]. For several years the potentiometric dye of choice for monitoring the ... More
Two-photon excitation of potentiometric probes enables optical recording of action potentials from mammalian nerve terminals in situ.
We report the first optical recordings of action potentials, in single trials, from one or a few (approximately 1-2 microm) mammalian nerve terminals in an intact in vitro preparation, the mouse neurohypophysis. The measurements used two-photon excitation along the ... More