DI-4 ANEPPDHQ
DI-4 ANEPPDHQ
Invitrogen™

DI-4 ANEPPDHQ

ANEP 染料是因环境中的电位变化而发出荧光的分子。这些为快速响应探针,因其电子结构的变化及其荧光特性的变化而运行,以应对周围电场的变化。它们的光学响应速度足以检测可激发细胞(包括单神经元、心脏细胞和完整大脑)的瞬时(毫秒)电位变化。然而,它们的电位依赖性荧光变化量级通常很小;快速响应探针通常显示了每了解更多信息
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货号数量
D368021 mg
货号 D36802
价格(CNY)
6,619.00
飞享价
Ends: 31-Dec-2026
9,066.00
共减 2,447.00 (27%)
Each
添加至购物车
数量:
1 mg
价格(CNY)
6,619.00
飞享价
Ends: 31-Dec-2026
9,066.00
共减 2,447.00 (27%)
Each
添加至购物车
ANEP 染料是因环境中的电位变化而发出荧光的分子。这些为快速响应探针,因其电子结构的变化及其荧光特性的变化而运行,以应对周围电场的变化。它们的光学响应速度足以检测可激发细胞(包括单神经元、心脏细胞和完整大脑)的瞬时(毫秒)电位变化。然而,它们的电位依赖性荧光变化量级通常很小;快速响应探针通常显示了每 100 mV 的 2-10% 荧光变化。此外,这些染料在激发光谱中显示出电位依赖性变化,因此可使用激发比测量法对膜电位进行定量测定。

了解更多有关离子指示剂(包括钙、钾、pH 值和膜电位指示剂)的信息›

电位敏感 ANEP 染料规格:
• 阳离子分子;显示内化水平极低且信噪比良好,并可用于显示模型膜中胆固醇富集脂质结构域
• 与模型磷脂膜结合的最大激发/发射波长为 ∼465/635 nm(但光谱特性高度依赖于环境)
• 与膜结合前不发荧光
• 可溶于乙醇、DMSO 和 DMF(二-2-ANEPEQ 为水溶性 ANEP 染料)
• 通过使用 Pluronic™ F-127 或逆行标记,将储备液直接添加至细胞培养基中,从而将染料引入细胞
• 快速响应探针,适用于检测亚毫秒膜电位变化


电位探针应用
由于 K+、Na+ 和 Cl- 浓度梯度(通过主动转运过程维持),细胞质膜的跨膜电位通常约为 -70 mV(内部为负)。电位探针可提供检测这些离子易位的间接方法。

膜电位增加和减少分别被称为膜超极化和去极化、在许多生理过程(包括神经脉冲传播、肌肉收缩、细胞信号转导和离子通道门控)中发挥核心作用。电位探针是研究这些过程的重要工具。

查找更多 ANEP 染料
我们提供多种形式的 ANEP 染料。有关这些探针的更多信息,请查看 Molecular Probes™ 手册中的快速响应探针 - 第 22.2 节

仅供科研使用。不可用于人或动物的治疗或诊断。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
数量1 mg
运输条件室温
子细胞定位细胞质&细胞溶质
颜色红外的
适用于(设备)荧光显微镜
产品类型ANEP 染料
Unit SizeEach
内容与储存
在冷冻冰箱(-5°C 至 -30°C)中避光储存。

常见问题解答 (FAQ)

当我使用膜电位指示剂时,看到神经元周围出现了较高的背景,如何降低背景干扰?

如果使用我们的FluoVolt 膜电势试剂盒(货号F10488),该试剂盒包含一种背景抑制剂,可改善这一问题。对于其他指标剂,可以考虑使用BackDrop 背景抑制剂(货号R37603、B10511和B10512)。

快反应膜电位探针和慢反应膜电位探针有什么区别?

在周围电场的作用下结构变化的分子可用作检测瞬时(毫秒级)电位变化的快反应探针。慢反应染料则会进入去极化细胞,结合蛋白或膜。增强去极化会造成额外的染料流入,增强荧光强度;过极化的特征则是荧光强度下降。快反应探针通常用于完整心脏组织的电位活动成像,或测量药理刺激引起的膜电位变化。慢反应探针常用于探索线粒体功能和细胞活力。

你们提供哪些类型的膜电位指示剂?我该如何根据自己的试验选择?

膜电位指示剂选择指南请见此处(https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-viability-and-regulation/ion-indicators/membrane-potential-indicators.html)。

I am seeing high background outside of my neuronal cells when using membrane potential indicators. What can I do to reduce background?

If you use our FluoVolt Membrane Potential Kit (Cat. No. F10488), the kit provides a background suppressor to reduce this problem. For other indicators, consider the use of BackDrop Background Suppressor (Cat no. R37603, B10511, and B10512).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between fast and slow-response membrane potential probes?

Molecules that change their structure in response to the surrounding electric field can function as fast-response probes for the detection of transient (millisecond) potential changes. Slow-response dyes function by entering depolarized cells and binding to proteins or membranes. Increased depolarization results in additional dye influx and an increase in fluorescence, while hyperpolarization is indicated by a decrease in fluorescence. Fast-response probes are commonly used to image electrical activity from intact heart tissues or measure membrane potential changes in response to pharmacological stimuli. Slow-responding probes are often used to explore mitochondrial function and cell viability.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (15)

引用和文献
Abstract
Novel naphthylstyryl-pyridium potentiometric dyes offer advantages for neural network analysis.
Authors:Obaid AL, Loew LM, Wuskell JP, Salzberg BM
Journal:J Neurosci Methods
PubMed ID:15003384
'The submucous plexus of the guinea pig intestine is a quasi-two-dimensional mammalian neural network that is particularly amenable to study using multiple site optical recording of transmembrane voltage (MSORTV) [Biol. Bull. 183 (1992) 344; J. Neurosci. 19 (1999) 3073]. For several years the potentiometric dye of choice for monitoring the ... More
Cholesterol-enriched lipid domains can be visualized by di-4-ANEPPDHQ with linear and nonlinear optics.
Authors:Jin L, Millard AC, Wuskell JP, Clark HA, Loew LM
Journal:Biophys J
PubMed ID:15879475
We present a membrane-staining dye, di-4-ANEPPDHQ, which differentiates liquid-ordered phases from liquid-disordered phases coexisting in model membranes under both linear and nonlinear microscopies. The dye's fluorescence emission spectrum is blue-shifted 60 nm in liquid-ordered phases compared with liquid-disordered phases, and shows strong second harmonic generation in the liquid-disordered phase compared ... More
Spectral characterization of the voltage-sensitive dye di-4-ANEPPDHQ applied to probing live primary and immortalized neurons.
Authors:Wang Y, Jing G, Perry S, Bartoli F, Tatic-Lucic S,
Journal:Opt Express
PubMed ID:19158915
Spectral properties of a recently developed voltage-sensitive dye, di-4-ANEPPDHQ, were characterized as the dye was dissolved in the solvent dimethyl sulfoxide as the stock solution, in Hank's buffered salt solution as the staining solution, and bound to the plasma membrane of primary rat hippocampal neurons and immortalized mouse hypothalamic neurons ... More
Laurdan and di-4-ANEPPDHQ do not respond to membrane-inserted peptides and are good probes for lipid packing.
Authors:Dinic J, Biverståhl H, Mäler L, Parmryd I,
Journal:Biochim Biophys Acta
PubMed ID:20937246
Laurdan and di-4-ANEPPDHQ are used as probes for membrane order, with a blue shift in emission for membranes in liquid-ordered (lo) phase relative to membranes in liquid-disordered (ld) phase. Their use as membrane order probes requires that their spectral shifts are unaffected by membrane proteins, which we have examined by ... More
Characterization and application of a new optical probe for membrane lipid domains.
Authors:Jin L, Millard AC, Wuskell JP, Dong X, Wu D, Clark HA, Loew LM
Journal:Biophys J
PubMed ID:16415047
In this article, we characterize the fluorescence of an environmentally sensitive probe for lipid membranes, di-4-ANEPPDHQ. In large unilamellar lipid vesicles (LUVs), its emission spectrum shifts up to 30 nm to the blue with increasing cholesterol concentration. Independently, it displays a comparable blue shift in liquid-ordered relative to liquid-disordered phases. ... More