Relocalization of phospholipase D activity mediates membrane formation during meiosis.
AuthorsRudge SA, Morris AJ, Engebrecht J
JournalJ Cell Biol
PubMed ID9425156
'Phospholipase D (PLD) enzymes catalyze the hydrolysis of phosphatidylcholine and are involved in membrane trafficking and cytoskeletal reorganization. The Saccharomyces cerevisiae SPO14 gene encodes a PLD that is essential for meiosis. We have analyzed the role of PLD in meiosis by examining two mutant proteins, one with a point mutation ... More
Phospholipase D mediates Fc gamma receptor activation of neutrophils and provides specificity between high-valency immune complexes and fMLP signaling pathways.
AuthorsGewirtz AT, Simons ER
JournalJ Leukoc Biol
PubMed ID9103240
'Neutrophils phagocytize high-valency immune complexes (HIC) by an Fc gamma receptor-mediated mechanism, activating an oxidative burst and initiating degranulation. In contrast, neutrophils exhibit chemotaxis to N-formylated peptides [e.g., N-formyl-methionyl-leucyl-phenylalanine (fMLP)] and secrete far fewer oxidants or granule contents than neutrophils activated by HIC. However, if neutrophils are treated with cytochalasin ... More
Phospholipase D activity in PC12 cells. Effects of overexpression of alpha2A-adrenergic receptors.
AuthorsElla KM, Qi C, McNair AF, Park JH, Wisehart-Johnson AE, Meier KE
JournalJ Biol Chem
PubMed ID9148895
'PC12 neuronal cells express a membrane phospholipase D (PLD) activity that is detected at similar levels in undifferentiated or differentiated cells. The regulation of this activity by agonists was explored. Membrane phospholipase D activity was increased by treatment of cells with the phorbol ester phorbol 12-myristate 13-acetate (PMA) or with ... More
Phosphatidylinositol-4,5-bisphosphate and phospholipase D-generated phosphatidic acid specify SNARE-mediated vesicle fusion for prospore membrane formation.
AuthorsMendonsa R, Engebrecht J,
JournalEukaryot Cell
PubMed ID19502581
'The soluble N-ethylmaleimide sensitive factor attachment protein receptor (SNARE) family of proteins is required for eukaryotic intracellular membrane fusions. Vesicle fusion for formation of the prospore membrane (PSM), a membrane compartment that forms de novo during yeast sporulation, requires SNARE function, phosphatidylinositol-4,5-bisphosphate [PI(4,5)P(2)], and the activity of the phospholipase D ... More
Characterization of a regulated form of phospholipase D in the yeast Saccharomyces cerevisiae.
AuthorsElla KM, Dolan JW, Meier KE
JournalBiochem J
PubMed ID7741711
'Phospholipase D (PLD), which is present in bacterial, plant and animal cells, can serve as an important element of signal-transduction pathways. This study examined the potential role of this enzyme in the regulation of Saccharomyces cerevisiae. An assay in vitro using a fluorescent 1-acyl-2-alkyl glycerophosphocholine as substrate was used to ... More
Characterization of Saccharomyces cerevisiae deficient in expression of phospholipase D.
AuthorsElla KM, Dolan JW, Qi C, Meier KE
JournalBiochem J
PubMed ID8660276
A gene encoding phospholipase D (PLD) in Saccharomyces cerevisiae was identified. The 195 kDa product of PLD1 has 24% overall sequence identity with a plant PLD. Expression of yeast PLD activity was eliminated by one-step gene disruption. Yeast haploids lacking PLD activity were deficient in growth on non-fermentable carbon sources. ... More
Human cytomegalovirus carries a cell-derived phospholipase A2 required for infectivity.
AuthorsAllal C, Buisson-Brenac C, Marion V, Claudel-Renard C, Faraut T, Dal Monte P, Streblow D, Record M, Davignon JL
JournalJ Virol
PubMed ID15220446
Human cytomegalovirus (HCMV) is known to carry host cell-derived proteins and mRNAs whose role in cell infection is not understood. We have identified a phospholipase A2 (PLA2) activity borne by HCMV by using an assay based on the hydrolysis of fluorescent phosphatidylcholine. This activity was found in all virus strains ... More
Activations of mitogen-activated protein kinases and phospholipase D in A7r5 vascular smooth muscle cells.
AuthorsJones LG, Ella KM, Bradshaw CD, Gause KC, Dey M, Wisehart-Johnson AE, Spivey EC, Meier KE
JournalJ Biol Chem
PubMed ID8089151
Activation of mitogen-activated protein kinases (MAPKs) was examined in the A7r5 rat vascular smooth muscle cell line. Treatment of A7r5 cells with vasopressin, phorbol ester (PMA), or serum resulted in activation of two MAPKs, Erk-1 and Erk-2. Phosphatidylinositol-specific phospholipase C was activated in response to vasopressin but not to PMA. ... More
A fluorescent assay for agonist-activated phospholipase D in mammalian cell extracts.
AuthorsElla KM, Meier GP, Bradshaw CD, Huffman KM, Spivey EC, Meier KE
JournalAnal Biochem
PubMed ID8053546
Phospholipase D (PLD) is activated in mammalian cells in response to a wide variety of stimuli. A rapid assay for agonist-activated PLD activity in cell extracts was developed, utilizing a fluorescent derivative of phosphatidylcholine as substrate. Utilization of the substrate was assessed following thin-layer chromatography of the reaction mixture. Hydrolysis ... More
Sequential actions of phospholipase D and phosphatidic acid phosphohydrolase 2b generate diglyceride in mammalian cells.
AuthorsSciorra VA, Morris AJ
JournalMol Biol Cell
PubMed ID10564277
Phosphatidylcholine (PC) is a major source of lipid-derived second messenger molecules that function as both intracellular and extracellular signals. PC-specific phospholipase D (PLD) and phosphatidic acid phosphohydrolase (PAP) are two pivotal enzymes in this signaling system, and they act in series to generate the biologically active lipids phosphatidic acid (PA) ... More
A HPLC-fluorescence detection method for determination of cardiac phospholipase D activity in vitro.
AuthorsKemken D, Mier K, Katus HA, Richardt G, Kurz T
JournalAnal Biochem
PubMed ID11067750
A nonradioactive assay for the investigation of phospholipase D (PLD) activity in cardiac membranes has been developed. A fluorescent derivative of phosphatidylcholine [2-decanoyl-1-(O-(11-(4,4-difluoro-5,7-dimethyl-4-bora-3a, 4a-diaza-s-indacene-3proprionyl)amino) undecyl) sn-glycero-3-phosphocholine] was utilized as substrate in an in vitro PLD-catalyzed transphosphatidylation reaction utilizing ethanol as second substrate. Unreacted phosphatidylcholine and the products of phospholipase activity ... More
Neutrophil degranulation and phospholipase D activation are enhanced if the Na+/H+ antiport is blocked.
AuthorsGewirtz AT, Seetoo KF, Simons ER
JournalJ Leukoc Biol
PubMed ID9665282
Neutrophils phagocytize high-valency immune complexes (HIC) by an Fc receptor-mediated mechanism. Engaging Fc receptors in this manner induces PMN to generate superoxide and release the contents of both their specific and azurophilic granules. Signaling events that precede and accompany PMN secretion include activation of phospholipase D (PLD), as well as ... More