Isolation and chemical characterization of Alzheimer's disease paired helical filament cytoskeletons: differentiation from amyloid plaque core protein.
AuthorsRoher AE, Palmer KC, Chau V, Ball MJ
JournalJ Cell Biol
PubMed ID3060472
'The paired helical filaments (PHFs) of Alzheimer''s disease were purified by a strategy in which the neurons and amyloid plaque cores of protein (APCP) were initially isolated. This was achieved by several steps of isocratic sucrose centrifugations of increasing molarity and a discontinuous isotonic Percoll density gradient. After collagenase elimination ... More
Carbocyanine dyes with long alkyl side-chains: broad spectrum inhibitors of mitochondrial electron transport chain activity.
AuthorsAnderson WM, Trgovcich-Zacok D
JournalBiochem Pharmacol
PubMed ID7763312
'Certain indocarbocyanine, thiacarbocyanine, and oxacarbocyanine dyes possessing short alkyl side-chains (one to five carbons) are potent inhibitors of mammalian mitochondrial NADH-ubiquinone reductase (EC 1.6.99.3) activity (Anderson et al., Biochem Pharmacol 41: 677-684, 1991; Anderson et al., Biochem Pharmacol 45: 691-696, 1993; Anderson et al., Biochem Pharmacol 45: 2115-2122, 1993), and ... More
Influence of hydralazine administration on oxygenation in spontaneous and transplanted tumor models.
AuthorsFenton BM
JournalInt J Radiat Oncol Biol Phys
PubMed ID11172963
'PURPOSE: To examine the effects of hydralazine on vascular perfusion and hypoxia in spontaneous vs. first generation and long-term transplanted murine tumor models. METHODS AND MATERIALS: Total anatomic blood vessels were quantified using image analysis of CD31 stained frozen sections, perfused vessels by i.v. injection of fluorescent DiOC(7), and tumor ... More
Characterization of a carbocyanine derivative as a fluorescent penetration probe.
AuthorsOlive PL, Durand RE
JournalCytometry
PubMed ID2448091
'The fluorescent carbocyanine dye 3,3-diheptyloxycarbocyanine [DiOC7(3)], originally described as a membrane potential probe, penetrates poorly into multicell spheroids. Since the dye is retained in the cells following spheroid disaggregation, cells can be selected from different depths within the spheroid using fluorescence-activated cell sorting. Characterization of the binding kinetics, stability, and ... More
The use of fluorescent probes to identify regions of transient perfusion in murine tumors.
AuthorsTrotter MJ, Chaplin DJ, Durand RE, Olive PL
JournalInt J Radiat Oncol Biol Phys
PubMed ID2703399
'Sequential intravenous injection of two fluorescent stains, Hoechst 33342 and DiOC7(3), can be used to quantify transient perfusion in experimental tumors. Regions of unmatched staining, indicative of intermittent perfusion, occur when vessels open or close in the 20 minute interval between administration of the dyes. In the murine SCCVII carcinoma, ... More
The ability of membrane potential dyes and calcafluor white to distinguish between viable and non-viable bacteria.
AuthorsMason DJ, Lopéz-Amorós R, Allman R, Stark JM, Lloyd D
JournalJ Appl Bacteriol
PubMed ID7537262
'Various dyes were assessed for their ability to discriminate between viable and non-viable bacteria. Two methods of killing were employed: by heat treatment or by gramicidin treatment. Staining was carried out in two ways; by staining directly in the medium or by washing cells prior to staining in buffer. Carbocyanine ... More
The respiratory response to heat shock in Neurospora crassa.
AuthorsHabel D, Plesofsky-Vig N, Brambl R
JournalFEMS Microbiol Lett
PubMed ID1833266
A sharp decrease in oxygen uptake occurred in Neurospora crassa cells that were transferred from 30 degrees C to 45 degrees C, and the respiration that resumed later at 45 degrees C was cyanide-insensitive. Energization of mitochondria, measured in vivo with fluorescence microscopy and a carbocyanine dye, also declined sharply ... More
Use of a carbocyanine dye as a marker of functional vasculature in murine tumours.
AuthorsTrotter MJ, Chaplin DJ, Olive PL
JournalBr J Cancer
PubMed ID2472164
An intravenously administered fluorescent carbocyanine dye, DiOC7(3), has been evaluated for use in conjunction with Hoechst 33342 as a marker of murine tumour vasculature. DiOC7(3) stains cells immediately adjacent to blood vessels and thus, like Hoechst 33342, outlines perfused tumour vasculature. The different fluorescence excitation and emission properties of DiOC7(3) ... More
Characterization of endoplasmic reticulum by co-localization of BiP and dicarbocyanine dyes.
AuthorsTerasaki M, Reese TS
JournalJ Cell Sci
PubMed ID1378452
The original concept of endoplasmic reticulum derived from the observation of a reticular network in cultured fibroblasts by electron microscopy of whole cells. It was previously reported that the fluorescent dye, DiOC6(3), stains a similar network as well as mitochondria and other organelles in living cells. Here, we investigate the ... More
A cyanine dye distinguishes between cycling and non-cycling fibroblasts.
AuthorsCohen RL, Muirhead KA, Gill JE, Waggoner AS, Horan PK
JournalNature
PubMed ID7219543
Cellular proliferative activity has previously been determined by measuring the incorporation of radiolabelled nucleotides or by visual inspection of cellular morphology. Although two flow cytometric methods have recently been developed which can distinguish cycling from non-cycling cells, both have serious disadvantages. One method requires uptake of a substantial amount of ... More
Kinetics of transport of dialkyloxacarbocyanines in multidrug-resistant cell lines overexpressing P-glycoprotein: interrelationship of dye alkyl chain length, cellular flux, and drug resistance.
AuthorsWadkins RM, Houghton PJ
JournalBiochemistry
PubMed ID7893683
The membrane transport properties of a series of dialkyloxacarbocyanine [DiOCn(3)] dyes in multidrug-resistant KB cell lines were investigated to determine the influence of alkyl chain length on the ability of p-glycoprotein (i) to protect cells from the toxicity of the dyes and (ii) to affect the plasma membrane flux of ... More
Transient perfusion in human melanoma xenografts.
AuthorsTufto I, Rofstad EK
JournalBr J Cancer
PubMed ID7536022
Studies of transplantable rodent tumours have suggested that malignant tissue might experience transient perfusion at the microvascular level. The purpose of the work reported here was to investigate whether transient perfusion can be demonstrated in xenografted human tumours. Tumours of four melanoma lines (A-07, D-12, R-18, U-25), grown orthotopically in ... More
Effect of vascular marker Hoechst 33342 on tumour perfusion and cardiovascular function in the mouse.
AuthorsTrotter MJ, Olive PL, Chaplin DJ
JournalBr J Cancer
PubMed ID2257217
The fluorescent stain Hoechst 33342 (H33342) has been employed extensively as an in vivo marker of functional tumour vasculature. We have found that H33342 causes a transient, dose-dependent decrease in tumour red blood cell (RBC) flow in SCCVII tumours as measured using laser Doppler flowmetry. After intravenous bolus injection of ... More
Polarity and reorganization of the endoplasmic reticulum during fertilization and ooplasmic segregation in the ascidian egg.
AuthorsSpeksnijder JE, Terasaki M, Hage WJ, Jaffe LF, Sardet C
JournalJ Cell Biol
PubMed ID8449980
During the first cell cycle of the ascidian egg, two phases of ooplasmic segregation create distinct cytoplasmic domains that are crucial for later development. We recently defined a domain enriched in ER in the vegetal region of Phallusia mammillata eggs. To explore the possible physiological and developmental function of this ... More