Formation of three-dimensional protein-lipid aggregates in monolayer films induced by surfactant protein B.
AuthorsKrol S, Ross M, Sieber M, Künneke S, Galla HJ, Janshoff A
JournalBiophys J
PubMed ID10920022
'This study focuses on the structural organization of surfactant protein B (SP-B) containing lipid monolayers. The artificial system is composed of the saturated phospholipids dipalmitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylglycerol (DPPG) in a molar ratio of 4:1 with 0.2 mol% SP-B. The different "squeeze-out" structures of SP-B were visualized by scanning probe ... More
Novel Fusogenic Liposomes for Fluorescent Cell Labeling and Membrane Modification.
AuthorsCsisza´r A, Hersch N, Dieluweit S, Biehl R, Merkel R, Hoffmann B,
JournalBioconjug Chem
PubMed ID20184308
'Efficient delivery of biomolecules into membranes of living cells as well as cell surface modifications are major biotechnological challenges. Here, novel liposome systems based on neutral and cationic lipids in combination with lipids modified by aromatic groups are introduced for such applications. The fusion efficiency of these liposome systems was ... More
Determination of the depth of BODIPY probes in model membranes by parallax analysis of fluorescence quenching.
AuthorsKaiser RD, London E
JournalBiochim Biophys Acta
PubMed ID9767081
'The location of a series of lipophilic and lipid-attached BODIPY (4, 4-difluoro-4-bora-3a,4a-diaza-s-indacene) membrane probes was analyzed by the quenching of BODIPY fluorescence by a series of nitroxide-labeled lipids in which the depth of the nitroxide group is varied. When attached to the polar headgroup of PE the BODIPY remained near ... More
The antimicrobial peptide trichogin and its interaction with phospholipid membranes.
AuthorsEpand RF, Epand RM, Monaco V, Stoia S, Formaggio F, Crisma M, Toniolo C
JournalEur J Biochem
PubMed ID10583397
'The interaction of the antimicrobial peptide trichogin GA IV with phospholipid bilayers has been studied. A series of analogs of trichogin was synthesized in which the nitroxide spin label, 4-amino-4-carboxy-2,2,6,6-tetramethylpiperidino-1-oxyl (TOAC), replaced one of the three alpha-aminoisobutyric acid (Aib) residues in the sequence. These modified peptides were used to assess ... More
Binding of endostatin to phosphatidylserine-containing membranes and formation of amyloid-like fibers.
AuthorsZhao H, Jutila A, Nurminen T, Wickström SA, Keski-Oja J, Kinnunen PK
JournalBiochemistry
PubMed ID15723529
'Endostatin, the 20-kDa C-terminal NC1 domain of collagen XVIII, is an endogenous inhibitor of tumor angiogenesis and tumor growth. A major problem in reconciling the many reported in vitro effects of endostatin is the lack of a high-affinity receptor, and a search for the latter continues. In accordance with the ... More
Depth-profiling with giant vesicle membranes.
AuthorsMenger FM, Keiper JS, Caran KL
JournalJ Am Chem Soc
PubMed ID12358515
Pharmacologic inhibition of phospholipid transfer protein activity reduces apolipoprotein-B secretion from hepatocytes.
AuthorsLuo Y, Shelly L, Sand T, Reidich B, Chang G, Macdougall M, Peakman MC, Jiang XC,
JournalJ Pharmacol Exp Ther
PubMed ID19933370
Phospholipid transfer protein (PLTP) plays an important role in atherogenesis, and its function goes well beyond that of transferring phospholipids between lipoprotein particles. Previous studies showed that genetic deficiency of PLTP in mice causes a substantially impaired hepatic secretion of apolipoprotein-B (apoB), the major protein of atherogenic lipoproteins. To understand ... More
Fluorescent lipid probes: some properties and applications (a review).
AuthorsMaier O, Oberle V, Hoekstra D
JournalChem Phys Lipids
PubMed ID12093532
Odd as it may seem, experimental challenges in lipid research are often hampered by the simplicity of the lipid structure. Since, as in protein research, mutants or overexpression of lipids are not realistic, a considerable amount of lipid research relies on the use of tagged lipid analogues. However, given the ... More
Purification and analysis of a phospholipase A2-like lytic factor of Trichomonas vaginalis.
AuthorsLubick KJ, Burgess DE
JournalInfect Immun
PubMed ID14977929
Trichomonas vaginalis produces soluble factors that have been reported to have the ability to damage target cells in vitro, and it has been hypothesized that these factors may play a role in the pathogenesis of human trichomoniasis. A lytic factor (LF) was purified from T. vaginalis, and the molecular characteristics ... More
The rate of lipid transfer during fusion depends on the structure of fluorescent lipid probes: a new chain-labeled lipid transfer probe pair.
AuthorsMalinin VS, Haque ME, Lentz BR
JournalBiochemistry
PubMed ID11444975
A number of fluorescent probes have been used to follow membrane fusion events, particularly intermixing of lipids. None of them is ideal. The most popular pair of probes is NBD-PE and Rh-PE, in which the fluorescent groups are attached to the lipid headgroups, making them sensitive to changes in the ... More
Endocytic sorting of lipid analogues differing solely in the chemistry of their hydrophobic tails.
AuthorsMukherjee S, Soe TT, Maxfield FR
JournalJ Cell Biol
PubMed ID10087269
To understand the mechanisms for endocytic sorting of lipids, we investigated the trafficking of three lipid-mimetic dialkylindocarbocyanine (DiI) derivatives, DiIC16(3) (1,1'-dihexadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate), DiIC12(3) (1,1'- didodecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate), and FAST DiI (1,1'-dilinoleyl-3,3,3', 3'-tetramethylindocarbocyanine perchlorate), in CHO cells by quantitative fluorescence microscopy. All three DiIs have the same head group, but differ in ... More
Spontaneous nucleotide exchange in low molecular weight GTPases by fluorescently labeled gamma-phosphate-linked GTP analogs.
Regulated guanosine nucleotide exchange and hydrolysis constitute the fundamental activities of low molecular weight GTPases. We show that three guanosine 5'-triphosphate analogs with BODIPY fluorophores coupled via the gamma phosphate bind to the GTPases Cdc42, Rac1, RhoA, and Ras and displace guanosine 5'-diphosphate with high intrinsic exchange rates in the ... More