'The spectroscopic properties of a new series of fatty acid analogs in which a dipyrrometheneboron difluoride fluorophore forms a segment of the acyl methylene chain are presented and their characteristics as fluorescent membrane probes are examined. When incorporated as a low mole fraction component in model phospholipid membranes, the probes ... More
Determination of the depth of BODIPY probes in model membranes by parallax analysis of fluorescence quenching.
AuthorsKaiser RD, London E
JournalBiochim Biophys Acta
PubMed ID9767081
'The location of a series of lipophilic and lipid-attached BODIPY (4, 4-difluoro-4-bora-3a,4a-diaza-s-indacene) membrane probes was analyzed by the quenching of BODIPY fluorescence by a series of nitroxide-labeled lipids in which the depth of the nitroxide group is varied. When attached to the polar headgroup of PE the BODIPY remained near ... More
The antimicrobial peptide trichogin and its interaction with phospholipid membranes.
AuthorsEpand RF, Epand RM, Monaco V, Stoia S, Formaggio F, Crisma M, Toniolo C
JournalEur J Biochem
PubMed ID10583397
'The interaction of the antimicrobial peptide trichogin GA IV with phospholipid bilayers has been studied. A series of analogs of trichogin was synthesized in which the nitroxide spin label, 4-amino-4-carboxy-2,2,6,6-tetramethylpiperidino-1-oxyl (TOAC), replaced one of the three alpha-aminoisobutyric acid (Aib) residues in the sequence. These modified peptides were used to assess ... More
Depth-profiling with giant vesicle membranes.
AuthorsMenger FM, Keiper JS, Caran KL
JournalJ Am Chem Soc
PubMed ID12358515
Fluorescent lipid probes: some properties and applications (a review).
AuthorsMaier O, Oberle V, Hoekstra D
JournalChem Phys Lipids
PubMed ID12093532
Odd as it may seem, experimental challenges in lipid research are often hampered by the simplicity of the lipid structure. Since, as in protein research, mutants or overexpression of lipids are not realistic, a considerable amount of lipid research relies on the use of tagged lipid analogues. However, given the ... More
Characterization of DNA/lipid complexes by fluorescence resonance energy transfer.
AuthorsMadeira C, Loura LM, Aires-Barros MR, Fedorov A, Prieto M
JournalBiophys J
PubMed ID14581211
Fluorescence resonance energy transfer (FRET) is a potential method for the characterization of DNA-cationic lipid complexes (lipoplexes). In this work, we used FRET models assuming a multilamellar lipoplex arrangement. The application of these models allows the determination of the distance between the fluorescent intercalator on the DNA and a membrane ... More
Purification and analysis of a phospholipase A2-like lytic factor of Trichomonas vaginalis.
AuthorsLubick KJ, Burgess DE
JournalInfect Immun
PubMed ID14977929
Trichomonas vaginalis produces soluble factors that have been reported to have the ability to damage target cells in vitro, and it has been hypothesized that these factors may play a role in the pathogenesis of human trichomoniasis. A lytic factor (LF) was purified from T. vaginalis, and the molecular characteristics ... More
Endocytic sorting of lipid analogues differing solely in the chemistry of their hydrophobic tails.
AuthorsMukherjee S, Soe TT, Maxfield FR
JournalJ Cell Biol
PubMed ID10087269
To understand the mechanisms for endocytic sorting of lipids, we investigated the trafficking of three lipid-mimetic dialkylindocarbocyanine (DiI) derivatives, DiIC16(3) (1,1'-dihexadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate), DiIC12(3) (1,1'- didodecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate), and FAST DiI (1,1'-dilinoleyl-3,3,3', 3'-tetramethylindocarbocyanine perchlorate), in CHO cells by quantitative fluorescence microscopy. All three DiIs have the same head group, but differ in ... More
Uptake and compartmentalization of fluorescent lipid analogs in larval Schistosoma mansoni.
AuthorsFurlong ST, Thibault KS, Morbelli LM, Quinn JJ, Rogers RA
JournalJ Lipid Res
PubMed ID7706934
Recent studies have suggested that host lipids are both a requirement for the human parasite Schistosoma mansoni and may play a role in evasion of host immunity. To study lipid utilization by this organism we have followed the uptake of fluorescent fatty acid and phospholipid analogs in two parasite stages, ... More