Bid, a widely expressed proapoptotic protein of the Bcl-2 family, displays lipid transfer activity.
AuthorsEsposti MD, Erler JT, Hickman JA, Dive C
JournalMol Cell Biol
PubMed ID11585909
'Bid is an abundant proapoptotic protein of the Bcl-2 family that is crucial for the induction of death receptor-mediated apoptosis in primary tissues such as liver. Bid action has been proposed to involve the relocation of its truncated form, tBid, to mitochondria to facilitate the release of apoptogenic cytochrome c. ... More
Intracellular transport of phosphatidic acid and phosphatidylcholine into lipid bodies in an oleaginous fungus, Mortierella ramanniana var. angulispora.
AuthorsKamisaka Y, Noda N
JournalJ Biochem (Tokyo)
PubMed ID11134953
'Exogenous fluorescent phosphatidic acid (PA) and phosphatidylcholine (PC) were transported into lipid bodies in an oleaginous fungus, Mortierella ramanniana var. angulispora [Kamisaka et al. (1999) Biochim. Biophys. Acta 1438, 185-198]. We further investigated the processes of fluorescent PA and PC transport into lipid bodies in this fungus by changing culture ... More
Exclusion of exogenous phosphatidylinositol-3,4,5-trisphosphate from neutrophil-polarizing pseudopodia: stabilization of the uropod and cell polarity.
AuthorsTian W, Laffafian I, Dewitt S, Hallett MB
JournalEMBO Rep
PubMed ID14528267
'Although there is accumulating evidence that the generation and localization of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P(3)) have important functions in neutrophil polarization and chemotaxis, the mechanism of this linkage has yet to be established. Here, using exogenous fluorescent PtdIns(3,4,5)P(3) introduced into the inner leaflet of the neutrophil plasma membrane by a cationic carrier, ... More
Enzymatic analysis of lipid phosphate phosphatases.
'Lipid phosphate monoesters including phosphatidic acid, lysophosphatidic acid, sphingosine 1-phosphate and ceramide 1-phosphate are intermediates in phosho- and sphingo-lipid biosynthesis and also play important roles in intra- and extra-cellular signaling. Dephosphorylation of these lipids terminates their signaling actions and, in some cases, generates products with additional biological activities or metabolic ... More
Sequence and functional similarities between pro-apoptotic Bid and plant lipid transfer proteins.
AuthorsDegli Esposti M
JournalBiochim Biophys Acta
PubMed ID11997142
'Pro-apoptotic proteins of the Bcl-2 family are known to act on mitochondria and facilitate the release of cytochrome c, but the biochemical mechanism of this action is unknown. Association with mitochondrial membranes is likely to be important in determining the capacity of releasing cytochrome c. The present work provides new ... More
Analyzing and modeling the inhibitory effect of phosphatidic acid on the GTP-gamma-S binding activity of Goalpha.
AuthorsQu L, Wan J, Cao Y, Zhang Y, Chen R, Huang Y,
JournalProteins
PubMed ID18175322
G proteins are the molecular switches of G-protein-coupled signal transmembrane transduction, which plays a pivotal role in diverse cellular processes. The guanine nucleotide binding states of Galpha-subunits are considered key factors for their functions. We report here that phosphatidic acid (PA) inhibits the [(35)S]-GTPgammaS binding activity of Goalpha. To elucidate ... More
A HPLC-fluorescence detection method for determination of phosphatidic acid phosphohydrolase activity: application in human myocardium.
AuthorsBurgdorf C, Prey A, Richardt G, Kurz T,
JournalAnal Biochem
PubMed ID18023403
Phosphatidic acid phosphohydrolase (PAP) catalyzes the dephosphorylation of phosphatidic acid (PA) to diacylglycerol, the second messenger responsible for activation of protein kinase C. Despite the crucial role of PAP lipid signaling, there are no data on PAP signaling function in the human heart. Here we present a nonradioactive assay for ... More
Role of phosphatidic acid phosphatase 2a in uptake of extracellular lipid phosphate mediators.
AuthorsRoberts RZ, Morris AJ
JournalBiochim Biophys Acta
PubMed ID10962286
Phosphatidic acid (PA) phosphatase 2a (PAP2a) is an integral membrane glycoprotein that hydrolyzes a number of structurally related lipid phosphate substrates when presented in mixed phospholipid and detergent micelles. The physiological substrate specificity and functions of this enzyme are unclear. Using reconstitution studies we demonstrate that PAP2a hydrolyses both PA ... More
Leukocyte lipid bodies: inflammation-related organelles are rapidly detected by wet scanning electron microscopy.
AuthorsMelo RC, Sabban A, Weller PF
JournalJ Lipid Res
PubMed ID16940552
Leukocyte lipid bodies are dynamic, functionally active organelles with central roles in inflammation. Here, we report that leukocyte lipid bodies are facilely detected by a versatile, potent technique, termed wet scanning electron microscopy (SEM), which combines the rapid preparation of light microscopy with the resolution of SEM. Using as leukocyte ... More
Characterization of DNA/lipid complexes by fluorescence resonance energy transfer.
AuthorsMadeira C, Loura LM, Aires-Barros MR, Fedorov A, Prieto M
JournalBiophys J
PubMed ID14581211
Fluorescence resonance energy transfer (FRET) is a potential method for the characterization of DNA-cationic lipid complexes (lipoplexes). In this work, we used FRET models assuming a multilamellar lipoplex arrangement. The application of these models allows the determination of the distance between the fluorescent intercalator on the DNA and a membrane ... More
A HPLC-fluorescence detection method for determination of cardiac phospholipase D activity in vitro.
AuthorsKemken D, Mier K, Katus HA, Richardt G, Kurz T
JournalAnal Biochem
PubMed ID11067750
A nonradioactive assay for the investigation of phospholipase D (PLD) activity in cardiac membranes has been developed. A fluorescent derivative of phosphatidylcholine [2-decanoyl-1-(O-(11-(4,4-difluoro-5,7-dimethyl-4-bora-3a, 4a-diaza-s-indacene-3proprionyl)amino) undecyl) sn-glycero-3-phosphocholine] was utilized as substrate in an in vitro PLD-catalyzed transphosphatidylation reaction utilizing ethanol as second substrate. Unreacted phosphatidylcholine and the products of phospholipase activity ... More
Dynamic features of prothrombin interaction with phospholipid vesicles of different size and composition: implications for protein--membrane contact.
AuthorsLu Y, Nelsestuen GL
JournalBiochemistry
PubMed ID8679573
The dynamics of prothrombin interaction with membrane vesicles of different size and composition was investigated to ascertain the impact of membrane surface characteristics and particle size on this interaction. Dissociation rates were highly sensitive to membrane composition and varied from about 20/s for membranes of 10% PS to 0.1/s for ... More
Ionic properties of membrane association by vitamin K-dependent proteins: the case for univalency.
AuthorsMcDonald JF, Evans TC, Emeagwali DB, Hariharan M, Allewell NM, Pusey ML, Shah AM, Nelsestuen GL
JournalBiochemistry
PubMed ID9398287
Ionic properties of membrane interaction by prothrombin, protein Z, and other vitamin K-dependent proteins were studied to determine the relevance of a monovalent membrane contact mechanism between one phospholipid headgroup and a calcium-lined pore in the protein [McDonald, J. F., Shah, A. M., Schwalbe, R. A., Kisiel, W., Dahlback, B., ... More
Lipid bodies and lipid body formation in an oleaginous fungus, Mortierella ramanniana var. angulispora.
AuthorsKamisaka Y, Noda N, Sakai T, Kawasaki K
JournalBiochim Biophys Acta
PubMed ID10320801
Mortierella ramanniana var. angulispora accumulates triacylglycerol (TG) in lipid bodies. Studies on lipid transport into lipid bodies are essential for elucidating mechanisms of lipid body formation. We used fluorescent dyes and fluorescent lipid analogs to visualize lipid body formation with a confocal laser scanning microscope. Different sizes of lipid bodies ... More