Oxidation of unsaturated phospholipids in membrane bilayer mixtures is accompanied by membrane fluidity changes.
AuthorsBorst JW, Visser NV, Kouptsova O, Visser AJ
JournalBiochim Biophys Acta
PubMed ID10962288
'Steady-state and time-resolved fluorescence spectroscopy has been used to obtain information on oxidation processes and associated dynamical and structural changes in model membrane bilayers made from single unilamellar vesicles (SUV''s) of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) mixed with increasing amounts of 1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphocholine (SAPC). The highly unsaturated arachidonoyl chain containing four double bonds is ... More
Islet amyloid polypeptide-induced membrane leakage involves uptake of lipids by forming amyloid fibers.
AuthorsSparr E, Engel MF, Sakharov DV, Sprong M, Jacobs J, de Kruijff B, Höppener JW, Killian JA
JournalFEBS Lett
PubMed ID15527771
Fibril formation of islet amyloid polypeptide (IAPP) is associated with cell death of the insulin-producing pancreatic beta-cells in patients with Type 2 Diabetes Mellitus. A likely cause for the cytotoxicity of human IAPP is that it destroys the barrier properties of the cell membrane. Here, we show by fluorescence confocal ... More
Clustering of class I HLA oligomers with CD8 and TCR: three-dimensional models based on fluorescence resonance energy transfer and crystallographic data.
AuthorsGáspár R, Bagossi P, Bene L, Matkó J, Szöllosi J, Tozsér J, Fésüs L, Waldmann TA, Damjanovich S
JournalJ Immunol
PubMed ID11290789
Fluorescence resonance energy transfer (FRET) data, in accordance with lateral mobility measurements, suggested the existence of class I HLA dimers and oligomers at the surface of live human cells, including the B lymphoblast cell line (JY) used in the present study. Intra- and intermolecular class I HLA epitope distances were ... More
The structural organization of cationic lipid-DNA complexes.
AuthorsWiethoff CM, Gill ML, Koe GS, Koe JG, Middaugh CR
JournalJ Biol Chem
PubMed ID12297507
The interaction of cationic liposomes with supercoiled plasmid DNA results in a major rearrangement of each component to form compact multilamellar structures comprised of alternating layers of two-dimensional arrays of DNA sandwiched between lipid bilayers. Fluorescence resonance energy transfer was used to estimate the distance of closest approach of DNA ... More
Purification and analysis of a phospholipase A2-like lytic factor of Trichomonas vaginalis.
AuthorsLubick KJ, Burgess DE
JournalInfect Immun
PubMed ID14977929
Trichomonas vaginalis produces soluble factors that have been reported to have the ability to damage target cells in vitro, and it has been hypothesized that these factors may play a role in the pathogenesis of human trichomoniasis. A lytic factor (LF) was purified from T. vaginalis, and the molecular characteristics ... More
Spontaneous, intervesicular transfer rates of fluorescent, acyl chain-labeled phosphatidylcholine analogs.
AuthorsElvington SM, Nichols JW
JournalBiochim Biophys Acta
PubMed ID17198675
It was recently shown that the structure of the fluorophore attached to the acyl chain of phosphatidylcholine analogs determines their mechanism of transport across the plasma membrane of yeast cells (Elvington et al., J. Biol Chem. 280:40957, 2005). In order to gain further insight into the physical properties of these ... More