Microspectroscopic imaging of nodulation factor-binding sites on living Vicia sativa roots using a novel bioactive fluorescent nodulation factor.
AuthorsGadella TW, Vereb G, Hadri AE, Röhrig H, Schmidt J, John M, Schell J, Bisseling T
JournalBiophys J
PubMed ID9129802
A novel bioactive fluorescent nodulation (Nod) factor, NodRlv-IV(BODIPY FL-C16), has been synthesized by attaching a BODIPY FL-C16 acyl chain to the primary amino group of chitotetraose deacetylated at the nonreducing terminus by recombinant NodB. The binding of the fluorescent Nod factor to root systems of Vicia sativa was investigated with ... More
Liver fatty acid-binding protein colocalizes with peroxisome proliferator activated receptor alpha and enhances ligand distribution to nuclei of living cells.
AuthorsHuang H, Starodub O, McIntosh A, Atshaves BP, Woldegiorgis G, Kier AB, Schroeder F
JournalBiochemistry
PubMed ID14992586
'Although it is hypothesized that long-chain fatty acyl CoAs (LCFA-CoAs) and long-chain fatty acids (LCFAs) regulate transcription in the nucleus, little is known regarding factors that determine the distribution of these ligands to nuclei of living cells. Immunofluorescence colocalization showed that liver fatty acid-binding protein (L-FABP; binds LCFA-CoA as well ... More
Detection of lipid peroxidation on erythrocytes using the excimer-forming property of a lipophilic BODIPY fluorescent dye.
AuthorsMakrigiorgos GM
JournalJ Biochem Biophys Methods
PubMed ID9310865
'Lipophilic analogues of dipyrrometheneboron (BODIPY-FL) dyes, used for membrane studies, normally fluoresce in the green wavelength region (approximately 516 nm), but at high local concentration, they shift their emission to the red region (approximately 540-600 nm) via excimer formation. A two-wavelength-based method is described that utilizes the excimer-forming property of ... More
AuthorsAtshaves BP, Storey SM, Huang H, Schroeder F
JournalMol Cell Biochem
PubMed ID15124915
'Although liver fatty acid binding protein (L-FABP) is known to enhance uptake and esterification of straight-chain fatty acids such as palmitic acid and oleic acid, its effects on oxidation and further metabolism of branched-chain fatty acids such as phytanic acid are not completely understood. The present data demonstrate for the ... More
Lateral diffusion of small compounds in human stratum corneum and model lipid bilayer systems.
AuthorsJohnson ME, Berk DA, Blankschtein D, Golan DE, Jain RK, Langer RS
JournalBiophys J
PubMed ID8913603
'An image-based technique of fluorescence recovery after photobleaching (video-FRAP) was used to measure the lateral diffusion coefficients of a series of nine fluorescent probes in two model lipid bilayer systems, dimyristoylphosphatidylcholine (DMPC) and DMPC/cholesterol (40 mol%), as well as in human stratum corneum-extracted lipids. The probes were all lipophilic, varied ... More
Differential partitioning of maternal fatty acid and phospholipid in neonate mosquito larvae.
AuthorsAtella GC, Shahabuddin M
JournalJ Exp Biol
PubMed ID12409488
'In animals, lipids are a source of energy, cell membrane components, signaling pathway modulators and emulsifying agents. In egg-laying animals, maternal yolk lipids, imported into the egg before laying, are metabolized or distributed in the developing embryo to serve these functions. Studies with birds, reptiles and insects have described lipid ... More
I-FABP expression alters the intracellular distribution of the BODIPY C16 fatty acid analog.
AuthorsKarsenty J, Helal O, de la Porte PL, Beauclair-Deprez P, Martin-Elyazidi C, Planells R, Storch J, Gastaldi M,
JournalMol Cell Biochem
PubMed ID19125316
To investigate the structure-function relationships of intestinal fatty acid-binding protein (I-FABP) in cellular fatty acid (FA) trafficking, we compared the distribution of a fluorescent FA analog (BODIPY FL C16) in Cos-1 cells transiently transfected with the wild type protein (wt I-FABP) to that of a variant deleted of the alpha ... More
Glucose Directly Links to Lipid Metabolism through High Affinity Interaction with Peroxisome Proliferator-activated Receptor {alpha}.
AuthorsHostetler HA, Huang H, Kier AB, Schroeder F,
JournalJ Biol Chem
PubMed ID18055466
The pathophysiology of diabetes is characterized not only by elevated glucose but also elevated long chain fatty acid levels. We show for the first time that the peroxisome proliferator-activated receptor-alpha (PPARalpha) binds glucose and glucose metabolites with high affinity, resulting in significantly altered PPARalpha secondary structure. Glucose decreased PPARalpha interaction ... More
Illumination of the malaria parasite Plasmodium falciparum alters intracellular pH. Implications for live cell imaging.
AuthorsWissing F, Sanchez CP, Rohrbach P, Ricken S, Lanzer M,
JournalJ Biol Chem
PubMed ID12140286
Live cell fluorescence microscopy has been widely used to study physiological processes in the human malarial parasite Plasmodium falciparum, including pH homeostasis, Ca(2+) signaling and protein targeting. However, the reproducibility of the data is often poor. Controversial statements exist regarding cytosolic and vacuolar baseline pH, as well as regarding the ... More
In vivo fluorescence correlation microscopy (FCM) reveals accumulation and immobilization of Nod factors in root hair cell walls.
Fluorescence correlation microscopy (FCM) is a new single-molecule detection technique based on the confocal principle to quantify molecular diffusion and concentration of fluorescent molecules (particles) with sub-micron resolution. In this study, FCM is applied to examine the diffusional behaviour of fluorescent Nod factor analogues on living Vicia sativa root hairs. ... More
Nod factors integrate spontaneously in biomembranes and transfer rapidly between membranes and to root hairs, but transbilayer flip-flop does not occur.
AuthorsGoedhart J, Röhrig H, Hink MA, van Hoek A, Visser AJ, Bisseling T, Gadella TW
JournalBiochemistry
PubMed ID10451386
Three novel nodulation (Nod) factors were synthesized from chitotetraose and three structurally different fluorescent BODIPY-tagged fatty acids. With fluorescence spectroscopic and microscopic techniques, the following aspects were studied: whether these amphiphilic molecules insert in membranes, whether they transfer between different membranes, and whether they are able to transfer from a ... More
Uptake and cellular localization of exogenous lipids by Giardia lamblia, a primitive eukaryote.
AuthorsStevens TL, Gibson GR, Adam R, Maier J, Allison-Ennis M, Das S
JournalExp Parasitol
PubMed ID9207743
Giardia lamblia trophozoites are unable to carry out de novo lipid synthesis. It is therefore likely that lipids are acquired from the small intestine of the host, in which the trophozoites are exposed to free and conjugated fatty acids, various sterols, phospholipids, bile acids, and bile-lipid mixed micelles. Here we ... More
Thiazolidinedione-induced activation of the transcription factor peroxisome proliferator-activated receptor gamma in cells adjacent to the murine skeletal muscle: implications for fibroblast functions.
AuthorsLöhrke B, Shahi SK, Krüger B, Schmidt P, Renne U, Dietl G
JournalPflugers Arch
PubMed ID10650980
Nuclear peroxisome proliferator-activated receptor gamma (PPARgamma) is the target of antidiabetogenic thiazolidinediones (TZD). However, recent studies failed to show that TZD has an effect in vitro on insulin-regulated glucose uptake in skeletal muscles, the major site of glucose disposal. The potential effects of TZD on cells adjacent to skeletal muscles ... More
Loss of SNORA73 reprograms cellular metabolism and protects against steatohepatitis.
Authors
JournalNat Commun
PubMed ID34471131
The Bone Marrow Protects and Optimizes Immunological Memory during Dietary Restriction.
AuthorsCollins N, Han SJ, Enamorado M, Link VM, Huang B, Moseman EA, Kishton RJ, Shannon JP, Dixit D, Schwab SR, Hickman HD, Restifo NP, McGavern DB, Schwartzberg PL, Belkaid Y
JournalCell
PubMed ID31442402
'Mammals evolved in the face of fluctuating food availability. How the immune system adapts to transient nutritional stress remains poorly understood. Here, we show that memory T cells collapsed in secondary lymphoid organs in the context of dietary restriction (DR) but dramatically accumulated within the bone marrow (BM), where they adopted ... More
IL-17A promotes fatty acid uptake through the IL-17A/IL-17RA/p-STAT3/FABP4 axis to fuel ovarian cancer growth in an adipocyte-rich microenvironment.
AuthorsYu C, Niu X, Du Y, Chen Y, Liu X, Xu L, Iwakura Y, Ma X, Li Y, Yao Z, Deng W
JournalCancer Immunol Immunother
PubMed ID31802182
'Pro-inflammatory cytokines are crucial mediators of cancer development, representing potential targets for cancer therapy. The molecular mechanism of a vital pro-inflammatory cytokine, IL-17A, in cancer progression and its potential use in therapy through influencing fatty acid (FA) metabolism, especially FA uptake of cancer cells, remains unknown. In the present study, ... More
Obesity Shapes Metabolism in the Tumor Microenvironment to Suppress Anti-Tumor Immunity.
Authors
JournalCell
PubMed ID33301708
Angiopoietin-2-integrin α5β1 signaling enhances vascular fatty acid transport and prevents ectopic lipid-induced insulin resistance.
Authors
JournalNat Commun
PubMed ID32532986
Visceral Adipose Tissue Immune Homeostasis Is Regulated by the Crosstalk between Adipocytes and Dendritic Cell Subsets.
Authors
JournalCell Metab
PubMed ID29514067
Neural metabolic imbalance induced by MOF dysfunction triggers pericyte activation and breakdown of vasculature.
Authors
JournalNat Cell Biol
PubMed ID32541879
Mitochondrial Integrity Regulated by Lipid Metabolism Is a Cell-Intrinsic Checkpoint for Treg Suppressive Function.
Authors
JournalCell Metab
PubMed ID31883840
Cell-programmed nutrient partitioning in the tumour microenvironment.
Authors
JournalNature
PubMed ID33828302
KIM-1 mediates fatty acid uptake by renal tubular cells to promote progressive diabetic kidney disease.
Authors
JournalCell Metab
PubMed ID33951465
A branched-chain amino acid metabolite drives vascular fatty acid transport and causes insulin resistance.
Authors
JournalNat Med
PubMed ID26950361
Distinct Influence of Saturated Fatty Acids on Malignant and Nonmalignant Human Lung Epithelial Cells.
AuthorsReczynska K, Khanal D, Pielichowska K, Pamula E, Chrzanowski W
JournalLipids
PubMed ID31970788
The impact of saturated fatty acids (FA) on viability and properties of malignant and nonmalignant cells has not been studied in detail so far. The present study was aimed at evaluation of the influence of saturated FA (10:0-18:0) on malignant (A459) and nonmalignant (BEAS-2B) human lung epithelial cells. FA strongly ... More
Germinal center B cells selectively oxidize fatty acids for energy while conducting minimal glycolysis.
AuthorsWeisel FJ, Mullett SJ, Elsner RA, Menk AV, Trivedi N, Luo W, Wikenheiser D, Hawse WF, Chikina M, Smita S, Conter LJ, Joachim SM, Wendell SG, Jurczak MJ, Winkler TH, Delgoffe GM, Shlomchik MJ
JournalNat Immunol
PubMed ID32066950
Germinal center B cells (GCBCs) are critical for generating long-lived humoral immunity. How GCBCs meet the energetic challenge of rapid proliferation is poorly understood. Dividing lymphocytes typically rely on aerobic glycolysis over oxidative phosphorylation for energy. Here we report that GCBCs are exceptional among proliferating B and T cells, as ... More
HNF4 Regulates Fatty Acid Oxidation and Is Required for Renewal of Intestinal Stem Cells in Mice.
AuthorsChen L, Vasoya RP, Toke NH, Parthasarathy A, Luo S, Chiles E, Flores J, Gao N, Bonder EM, Su X, Verzi MP
JournalGastroenterology
PubMed ID31759926
Functions of intestinal stem cells (ISCs) are regulated by diet and metabolic pathways. Hepatocyte nuclear factor 4 (HNF4) family are transcription factors that bind fatty acids. We investigated how HNF4 transcription factors regulate metabolism and their functions in ISCs in mice. ... More