Triacylglycerol is synthesized in a specific subclass of caveolae in primary adipocytes.
AuthorsOst A, Ortegren U, Gustavsson J, Nystrom FH, Strålfors P
JournalJ Biol Chem
PubMed ID15537657
'A principal metabolic function of adipocytes is to synthesize triacylglycerol (TG) from exogenous fatty acids. The level of fatty acids has to be tightly controlled in the adipocyte, as they can act as detergents that rapidly dissolve the plasma membrane, causing cell lysis if allowed to accumulate. Fatty acids therefore ... More
AuthorsHara T, Hirasawa A, Sun Q, Koshimizu TA, Itsubo C, Sadakane K, Awaji T, Tsujimoto G,
JournalMol Pharmacol
PubMed ID18927207
'GPR40 is a G protein-coupled receptor (GPCR) whose endogenous ligands have recently been identified as medium- and long-chain free fatty acids (FFAs), and it is thought to play an important role in insulin release. Despite recent research efforts, much still remains unclear in our understanding of its pharmacology, mainly because ... More
A novel fluorescent fatty acid, 5-methyl-BDY-3-dodecanoic acid, is a potential probe in lipid transport studies by incorporating selectively to lipid classes of BHK cells.
AuthorsKasurinen J
JournalBiochem Biophys Res Commun
PubMed ID1417832
'The 5-methyl-BDY-3-dodecanoic acid (B12FA) labelling of BHK cell lipids was analyzed by thin layer and reverse phase column chromatography. Incorporation to phospholipids was selective: over 90% of B12FA label was enriched in phosphatidylcholine. The major molecular species of PC was that containing palmitate as the unlabelled fatty acid. Small amounts ... More
Determination of the depth of BODIPY probes in model membranes by parallax analysis of fluorescence quenching.
AuthorsKaiser RD, London E
JournalBiochim Biophys Acta
PubMed ID9767081
'The location of a series of lipophilic and lipid-attached BODIPY (4, 4-difluoro-4-bora-3a,4a-diaza-s-indacene) membrane probes was analyzed by the quenching of BODIPY fluorescence by a series of nitroxide-labeled lipids in which the depth of the nitroxide group is varied. When attached to the polar headgroup of PE the BODIPY remained near ... More
Mode of action of an antiviral peptide from HIV-1. Inhibition at a post-lipid mixing stage.
AuthorsKliger Y, Gallo SA, Peisajovich SG, Munoz-Barroso I, Avkin S, Blumenthal R, Shai Y
JournalJ Biol Chem
PubMed ID11027678
'DP178, a synthetic peptide corresponding to a segment of the transmembrane envelope glycoprotein (gp41) of human immunodeficiency virus, type 1 (HIV-1), is a potent inhibitor of viral infection and virus-mediated cell-cell fusion. Nevertheless, DP178 does not contain gp41 coiled-coil cavity binding residues postulated to be essential for inhibiting HIV-1 entry. ... More
Sequence and functional similarities between pro-apoptotic Bid and plant lipid transfer proteins.
AuthorsDegli Esposti M
JournalBiochim Biophys Acta
PubMed ID11997142
'Pro-apoptotic proteins of the Bcl-2 family are known to act on mitochondria and facilitate the release of cytochrome c, but the biochemical mechanism of this action is unknown. Association with mitochondrial membranes is likely to be important in determining the capacity of releasing cytochrome c. The present work provides new ... More
The emergence of competition between model protocells.
AuthorsChen IA, Roberts RW, Szostak JW
JournalScience
PubMed ID15353806
The transition from independent molecular entities to cellular structures with integrated behaviors was a crucial aspect of the origin of life. We show that simple physical principles can mediate a coordinated interaction between genome and compartment boundary, independent of any genomic functions beyond self-replication. RNA, encapsulated in fatty acid vesicles, ... More
Disruption of the Saccharomyces cerevisiae homologue to the murine fatty acid transport protein impairs uptake and growth on long-chain fatty acids.
AuthorsFaergeman NJ, DiRusso CC, Elberger A, Knudsen J, Black PN
JournalJ Biol Chem
PubMed ID9079682
The yeast Saccharomyces cerevisiae is able to utilize exogenous fatty acids for a variety of cellular processes including beta-oxidation, phospholipid biosynthesis, and protein modification. The molecular mechanisms that govern the uptake of these compounds in S. cerevisiae have not been described. We report the characterization of FAT1, a gene that ... More
FATP1 channels exogenous FA into 1,2,3-triacyl-sn-glycerol and down-regulates sphingomyelin and cholesterol metabolism in growing 293 cells.
AuthorsHatch GM, Smith AJ, Xu FY, Hall AM, Bernlohr DA
JournalJ Lipid Res
PubMed ID12235169
Biosynthesis of lipids was investigated in growing 293 cells stably expressing fatty acid (FA) transport protein 1 (FATP1), a bifunctional polypeptide with FA transport as well as fatty acyl-CoA synthetase activity. In short-term (30 s) incubations, FA uptake was increased in FATP1 expressing cells (C8 cells) compared with the vector ... More
A live-cell high-throughput screening assay for identification of fatty acid uptake inhibitors.
AuthorsLi H, Black PN, DiRusso CC
JournalAnal Biochem
PubMed ID15582553
We developed a live-cell high-throughput assay system using the baker's yeast Saccharomyces cerevisiae to screen for chemical compounds that will inhibit fatty acid uptake. The target for the inhibitors is a mammalian fatty acid transport protein (mmFATP2), which is involved in the fatty acid transport and activation pathway. The mmFATP2 ... More
Fatty acid-induced cholecystokinin secretion and changes in intracellular Ca2+ in two enteroendocrine cell lines, STC-1 and GLUTag.
AuthorsSidhu SS, Thompson DG, Warhurst G, Case RM, Benson RS
JournalJ Physiol
PubMed ID11018115
1. Fatty acid-induced cholecystokinin (CCK) secretion in humans and from the enteroendocrine cell line STC-1 depends critically on acyl chain length. 2. Therefore we have characterized the relationship between acyl chain length and the potency of the fatty acid to induce CCK secretion and changes in intracellular Ca2+ concentration ([Ca2+]i) ... More
The Acyl-CoA synthetases encoded within FAA1 and FAA4 in Saccharomyces cerevisiae function as components of the fatty acid transport system linking import, activation, and intracellular Utilization.
AuthorsFaergeman NJ, Black PN, Zhao XD, Knudsen J, DiRusso CC
JournalJ Biol Chem
PubMed ID11477098
Exogenous long-chain fatty acids are activated to coenzyme A derivatives prior to metabolic utilization. In the yeast Saccharomyces cerevisiae, the activation of these compounds prior to metabolic utilization proceeds through the fatty acyl-CoA synthetases Faa1p and Faa4p. Faa1p or Faa4p are essential for long-chain fatty acid import, suggesting that one ... More
Lipid and membrane protein transfer from human neutrophils to schistosomes is mediated by ligand binding.
AuthorsRogers RA, Jack RM, Furlong ST
JournalJ Cell Sci
PubMed ID8282756
Attachment of human neutrophils to schistosomula of Schistosoma mansoni involves leukocyte receptors recognizing carbohydrate, complement and/or IgG ligands on the parasite surface. Here, we examined the transfer of a fluorescent fatty acid analog (BOFA) from human neutrophils to schistosomula coated with concanavalin A (Con A), immune serum or nonimmune serum ... More