AuthorsHara T, Hirasawa A, Sun Q, Koshimizu TA, Itsubo C, Sadakane K, Awaji T, Tsujimoto G,
JournalMol Pharmacol
PubMed ID18927207
'GPR40 is a G protein-coupled receptor (GPCR) whose endogenous ligands have recently been identified as medium- and long-chain free fatty acids (FFAs), and it is thought to play an important role in insulin release. Despite recent research efforts, much still remains unclear in our understanding of its pharmacology, mainly because ... More
Determination of the depth of BODIPY probes in model membranes by parallax analysis of fluorescence quenching.
AuthorsKaiser RD, London E
JournalBiochim Biophys Acta
PubMed ID9767081
'The location of a series of lipophilic and lipid-attached BODIPY (4, 4-difluoro-4-bora-3a,4a-diaza-s-indacene) membrane probes was analyzed by the quenching of BODIPY fluorescence by a series of nitroxide-labeled lipids in which the depth of the nitroxide group is varied. When attached to the polar headgroup of PE the BODIPY remained near ... More
Unilamellar vesicle formation and encapsulation by microfluidic jetting.
AuthorsStachowiak JC, Richmond DL, Li TH, Liu AP, Parekh SH, Fletcher DA,
JournalProc Natl Acad Sci U S A
PubMed ID18353990
'Compartmentalization of biomolecules within lipid membranes is a fundamental requirement of living systems and an essential feature of many pharmaceutical therapies. However, applications of membrane-enclosed solutions of proteins, DNA, and other biologically active compounds have been limited by the difficulty of forming unilamellar vesicles with controlled contents in a repeatable ... More