Manipulating FRET with polymeric vesicles: development of a "mix-and-detect" type fluorescence sensor for bacterial toxin.
AuthorsMa G, Cheng Q
JournalLangmuir
PubMed ID16863214
'A simple "mix-and-detect" type of fluorescence sensor for cholera toxin (CT) is reported. The sensor consists of a BODIPY lipid dye and polydiacetylene (PDA) vesicles and utilizes the lipid insertion and FRET mechanism to offer a direct and fluorescence "turn-on" detection of the analyte. BODIPY conjugated GM1, dissolved in a ... More
Photolysis of caged phosphatidic acid induces flagellar excision in Chlamydomonas.
AuthorsGoedhart J, Gadella TW
JournalBiochemistry
PubMed ID15065870
'Phosphatidic (PtdOH) acid formation is recognized as an important step in numerous signaling pathways in both plants and mammals. To study the role of this lipid in signaling pathways, it is of major interest to be able to increase the amount of this lipid directly. Therefore, "caged" PtdOH was synthesized, ... More
The emergence of competition between model protocells.
AuthorsChen IA, Roberts RW, Szostak JW
JournalScience
PubMed ID15353806
The transition from independent molecular entities to cellular structures with integrated behaviors was a crucial aspect of the origin of life. We show that simple physical principles can mediate a coordinated interaction between genome and compartment boundary, independent of any genomic functions beyond self-replication. RNA, encapsulated in fatty acid vesicles, ... More
Altered lipid droplet dynamics in hepatocytes lacking triacylglycerol hydrolase expression.
AuthorsWang H, Wei E, Quiroga AD, Sun X, Touret N, Lehner R,
JournalMol Biol Cell
PubMed ID20410140
Lipid droplets (LDs) form from the endoplasmic reticulum (ER) and grow in size by obtaining triacylglycerols (TG). Triacylglycerol hydrolase (TGH), a lipase residing in the ER, is involved in the mobilization of TG stored in LDs for the secretion of very-low-density lipoproteins. In this study, we investigated TGH-mediated changes in ... More
Fluorogenic substrates for high-throughput measurements of endothelial lipase activity.
AuthorsMitnaul LJ, Tian J, Burton C, Lam MH, Zhu Y, Olson SH, Schneeweis JE, Zuck P, Pandit S, Anderson M, Maletic MM, Waddell ST, Wright SD, Sparrow CP, Lund EG
JournalJ Lipid Res
PubMed ID17090660
Endothelial lipase (EL) has been shown to be a critical determinant for high density lipoprotein cholesterol levels in vivo; therefore, assays that measure EL activity have become important for the discovery of small molecule inhibitors that specifically target EL. Here, we describe fluorescent Bodipy-labeled substrates that can be used in ... More
In vivo fluorescence correlation microscopy (FCM) reveals accumulation and immobilization of Nod factors in root hair cell walls.
Fluorescence correlation microscopy (FCM) is a new single-molecule detection technique based on the confocal principle to quantify molecular diffusion and concentration of fluorescent molecules (particles) with sub-micron resolution. In this study, FCM is applied to examine the diffusional behaviour of fluorescent Nod factor analogues on living Vicia sativa root hairs. ... More
Nod factors integrate spontaneously in biomembranes and transfer rapidly between membranes and to root hairs, but transbilayer flip-flop does not occur.
AuthorsGoedhart J, Röhrig H, Hink MA, van Hoek A, Visser AJ, Bisseling T, Gadella TW
JournalBiochemistry
PubMed ID10451386
Three novel nodulation (Nod) factors were synthesized from chitotetraose and three structurally different fluorescent BODIPY-tagged fatty acids. With fluorescence spectroscopic and microscopic techniques, the following aspects were studied: whether these amphiphilic molecules insert in membranes, whether they transfer between different membranes, and whether they are able to transfer from a ... More
Microsomal triglyceride transfer protein is required for yolk lipid utilization and absorption of dietary lipids in zebrafish larvae.
AuthorsSchlegel A, Stainier DY
JournalBiochemistry
PubMed ID17176039
Although the absorption, transport, and catabolism of dietary lipids have been studied extensively in great detail in mammals and other vertebrates, a tractable genetic system for identifying novel genes involved in these physiologic processes is not available. To establish such a model, we monitored neutral lipid by staining fixed zebrafish ... More
Lipid domains of mycobacteria studied with fluorescent molecular probes.
AuthorsChristensen H, Garton NJ, Horobin RW, Minnikin DE, Barer MR
JournalMol Microbiol
PubMed ID10200973
The complex mycobacterial cell envelope is recognized as a critical factor in our failure to control tuberculosis, leprosy and other non-tuberculous pathogens. Although its composition has been extensively determined, many details regarding the organization of the envelope remain uncertain. This is particularly so for the non-covalently bound lipids, whose natural ... More
Adipocytes disrupt the translational programme of acute lymphoblastic leukaemia to favour tumour survival and persistence.
Authors
JournalNat Commun
PubMed ID34535653
An ESCRT-dependent step in fatty acid transfer from lipid droplets to mitochondria through VPS13D-TSG101 interactions.
Authors
JournalNat Commun
PubMed ID33623047
The role of NSP6 in the biogenesis of the SARS-CoV-2 replication organelle.
Authors
JournalNature
PubMed ID35551511
The autophagy protein ATG9A enables lipid mobilization from lipid droplets.
Authors
JournalNat Commun
PubMed ID34799570
RIPK3 Orchestrates Fatty Acid Metabolism in Tumor-Associated Macrophages and Hepatocarcinogenesis.
Authors
JournalCancer Immunol Res
PubMed ID32122992
STIM1 is a metabolic checkpoint regulating the invasion and metastasis of hepatocellular carcinoma.
Authors
JournalTheranostics
PubMed ID32483465
Sterols are required for the coordinated assembly of lipid droplets in developing seeds.
Authors
JournalNat Commun
PubMed ID34552075
Colloidal crystals with diamond symmetry at optical lengthscales.
Authors
JournalNat Commun
PubMed ID28194025
MYCN-driven fatty acid uptake is a metabolic vulnerability in neuroblastoma.
Authors
JournalNat Commun
PubMed ID35764645
Cell Clustering Promotes a Metabolic Switch that Supports Metastatic Colonization.