引用和文献 (47)

Invitrogen™

BODIPY™ 581/591 C11（脂质过氧化传感器）

脂质过氧化传感器

货号	数量
D3861	1 mg

货号 D3861

价格（CNY)

2,179.00

Ends: 31-Dec-2026

2,984.00

共减 805.00 (27%)

价格（CNY)

2,179.00

Ends: 31-Dec-2026

2,984.00

共减 805.00 (27%)

BODIPY™ 581/591 C11 undecanoic acid (Lipid Peroxidation Sensor) is a sensitive fluorescent probe for lipid peroxidation assays that localizes to membranes in live cells. Oxidation of the polyunsaturated butadienyl portion of this fatty acid analog in live cells results in a shift of the fluorescence emission peak from red (∼590 nm) to green (∼510 nm), allowing ratiometric analysis of lipid peroxidation using fluorescence microscopy, high-content analysis, and flow cytometry. In the reduced state, the excitation and emission maxima of BODIPY 581/591 C11 is 581/591 nm; after oxidation, the probe shifts the excitation and emission to 488/510 nm.

BODIPY™ 581/591 十一烷酸可用于检测细胞和细胞膜中的活性氧 (ROS)。染料的多不饱和丁二烯基部分的氧化导致荧光发射峰从∼590 nm移动到∼510 nm。在还原状态下，BODIPY™ 581/591 十一烷酸的激发和发射最大值为 581/591 nm；氧化后，激发和发射最大值移至 488/510 nm。

该产品我们建议用高质量无水 DMSO 溶解，母液浓度 1 至 10 mM，≤−20°C 避光保存。

仅供科研使用。不可用于诊断程序。

数量1 mg

检测方法Fluorescence

激发/发射581/591 nm

指示器ROS

产品线BODIPY

产品类型Lipid Sensor

Unit SizeEach

常见问题解答 (FAQ)

What solvent should be used to reconstitute/dissolve BODIPY 581/591 C11 (Lipid Peroxidation Sensor) (Cat. No. D3861)?

For BODIPY 581/591 C11 (Cat No. D3861), we recommend that you dissolve this in dimethyl sulfoxide (DMSO). You can prepare a stock solution in any concentration between 1 and 5 mg/mL. Please note that the BODIPY/DMSO stock solution must be stored at -20 degrees C, desiccated and protected from light.

Find additional tips, troubleshooting help, and resources within our Cell Imaging Support Center.

引用和文献 (47)

引用和文献

Abstract

Cell death during ischemia: relationship to mitochondrial depolarization and ROS generation.

Authors:Levraut J, Iwase H, Shao ZH, Vanden Hoek TL, Schumacker PT

Journal:Am J Physiol Heart Circ Physiol

PubMed ID:12388276

Ischemia-reperfusion injury induces cell death, but the responsible mechanisms are not understood. This study examined mitochondrial depolarization and cell death during ischemia and reperfusion. Contracting cardiomyocytes were subjected to 60-min ischemia followed by 3-h reperfusion. Mitochondrial membrane potential (DeltaPsi(m)) was assessed with tetramethylrhodamine methyl ester. During ischemia, DeltaPsi(m) decreased to ... More

Improved efficacy of stem cell labeling for magnetic resonance imaging studies by the use of cationic liposomes.

Authors:van den Bos EJ, Wagner A, Mahrholdt H, Thompson RB, Morimoto Y, Sutton BS, Judd RM, Taylor DA

Journal:Cell Transplant

PubMed ID:14653621

'Labeling stem cells with FDA-approved superparamagnetic iron oxide particles makes it possible to track cells in vivo with magnetic resonance imaging (MRI), but high intracellular levels of iron can cause free radical formation and cytotoxicity. We hypothesized that the use of cationic liposomes would increase labeling efficiency without toxic effects. ... More

Action of DCFH and BODIPY as a probe for radical oxidation in hydrophilic and lipophilic domain.

Authors:Yoshida Y, Shimakawa S, Itoh N, Niki E

Journal:Free Radic Res

PubMed ID:14567446

'Fluorogenic probes such as 2'',7''-dichlorofluorescin (DCFH) have been extensively used to detect oxidative events and to measure antioxidant capacity. At the same time, however, the inherent drawbacks of these probes such as non-specificity towards oxidizing species have been pointed out. The present study was carried out to analyze the action ... More

A method to measure the oxidizability of both the aqueous and lipid compartments of plasma.

Authors:Aldini G, Yeum KJ, Russell RM, Krinsky NI

Journal:Free Radic Biol Med

PubMed ID:11677037

'The lipophilic radical initiator (MeO-AMVN) and the fluorescent probe C11BODIPY581/591 (BODIPY) were used to measure the lipid compartment oxidizability of human plasma. Aqueous plasma oxidizability was initiated by the aqueous peroxyl radical generator, AAPH, and 2'',7''-dichlorodihydrofluorescein (DCFH) was employed as the marker of the oxidative reaction. The distribution in aqueous ... More

Detection of lipid peroxidation in equine spermatozoa based upon the lipophilic fluorescent dye C1l-BODIPY581/591.

Authors:Ball BA, Vo A

Journal:J Androl

PubMed ID:11868820

'The lipophilic fluorescent probe, 4,4-difluoro-5-(4-phenyl-1 ,3-butadienyl)-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid (C11-BODIPY581/591) was used to evaluate changes in lipid peroxidation in equine spermatozoa during both short-term exposure to ferrous sulfate and sodium ascorbate in the presence of cumene hydroperoxide as well as during storage of spermatozoa at 5 degrees C for 48 hours. Peroxidation ... More

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