Ischemia-reperfusion injury induces cell death, but the responsible mechanisms are not understood. This study examined mitochondrial depolarization and cell death during ischemia and reperfusion. Contracting cardiomyocytes were subjected to 60-min ischemia followed by 3-h reperfusion. Mitochondrial membrane potential (DeltaPsi(m)) was assessed with tetramethylrhodamine methyl ester. During ischemia, DeltaPsi(m) decreased to ... More
Temperature dependence of cell-cell fusion induced by the envelope glycoprotein of human immunodeficiency virus type 1.
AuthorsFrey S, Marsh M, Günther S, Pelchen-Matthews A, Stephens P, Ortlepp S, Stegmann T
JournalJ Virol
PubMed ID7853478
'We investigated cell-cell fusion induced by the envelope glycoprotein of human immunodeficiency virus type 1 strain IIIB expressed on the surface of CHO cells. These cells formed syncytia when incubated together with CD4-positive human lymphoblastoid SupT1 cells or HeLa-CD4 cells but not when incubated with CD4-negative cell lines. A new ... More
A real-time fluorogenic phospholipase A(2) assay for biochemical and cellular activity measurements.
'A fluorogenic analog of the PLA(2) substrate PC, named Dabcyl-BODIPY-PC, or simply DBPC, was synthesized with a fluorescence quencher (Dabcyl, 4-[(4-[N,N-dimethylamino]phenyl)azo]benzoic acid) in the sn-1 acyl chain and a BODIPY fluor in the sn-2 acyl chain. DBPC was recognized by sPLA(2) from each of the four sources examined (bee venom, ... More
Fluorogenic substrates for high-throughput measurements of endothelial lipase activity.
AuthorsMitnaul LJ, Tian J, Burton C, Lam MH, Zhu Y, Olson SH, Schneeweis JE, Zuck P, Pandit S, Anderson M, Maletic MM, Waddell ST, Wright SD, Sparrow CP, Lund EG
JournalJ Lipid Res
PubMed ID17090660
Endothelial lipase (EL) has been shown to be a critical determinant for high density lipoprotein cholesterol levels in vivo; therefore, assays that measure EL activity have become important for the discovery of small molecule inhibitors that specifically target EL. Here, we describe fluorescent Bodipy-labeled substrates that can be used in ... More