Up-regulation of calcitonin gene-related peptide and receptor tyrosine kinase TrkB in rat bladder afferent neurons following TNBS colitis.
AuthorsQiao LY, Grider JR
JournalExp Neurol
PubMed ID17303123
'Colonic inflammation has profound effects on the urinary bladder physiology and produces hypersensitivity of bladder afferent neurons and neurogenic bladder overactivity. Calcitonin gene-related peptide (CGRP) expressed in dorsal root ganglia (DRG) plays an important role in mediating sensory perception following visceral inflammation. In the present study, we determined that the ... More
Nonvagal origin of galanin-containing nerve terminals innervating striated muscle fibers of the rat esophagus.
AuthorsWörl J, Fischer J, Neuhuber WL
JournalCell Tissue Res
PubMed ID9582402
'We investigated the origin of galanin-positive nerve fibers on motor endplates in rat esophagus using anterograde 1,1''-dioleyl-3,3,3'', 3''-tetramethylindocarbocyanine methane sulfonate (DiI) tracing from the nucleus ambiguus combined with galanin immunocytochemistry and calcitonin gene-related peptide immunocytochemistry. To demonstrate spatial relationships of galanin-positive nerve fibers to vagal and enteric nerve fibers on ... More
Carbocyanine dyes with long alkyl side-chains: broad spectrum inhibitors of mitochondrial electron transport chain activity.
AuthorsAnderson WM, Trgovcich-Zacok D
JournalBiochem Pharmacol
PubMed ID7763312
'Certain indocarbocyanine, thiacarbocyanine, and oxacarbocyanine dyes possessing short alkyl side-chains (one to five carbons) are potent inhibitors of mammalian mitochondrial NADH-ubiquinone reductase (EC 1.6.99.3) activity (Anderson et al., Biochem Pharmacol 41: 677-684, 1991; Anderson et al., Biochem Pharmacol 45: 691-696, 1993; Anderson et al., Biochem Pharmacol 45: 2115-2122, 1993), and ... More
Differential control over postganglionic neurons in rat cardiac ganglia by NA and DmnX neurons: anatomical evidence.
AuthorsCheng Z, Zhang H, Guo SZ, Wurster R, Gozal D
JournalAm J Physiol Regul Integr Comp Physiol
PubMed ID14644755
In previous single-labeling experiments, we showed that neurons in the nucleus ambiguous (NA) and the dorsal moto nucleus of the vagus (DmnX) project to intrinsic cardiac ganglia. Neurons in these two motor nuclei differ significantly in the size of their projection fields, axon caliber, and endings in cardiac ganglia. These ... More
Anterograde tracing method using DiI to label vagal innervation of the embryonic and early postnatal mouse gastrointestinal tract.
AuthorsMurphy MC, Fox EA
JournalJ Neurosci Methods
PubMed ID17418900
The mouse is an extremely valuable model for studying vagal development in relation to strain differences, genetic variation, gene manipulations or pharmacological manipulations. Therefore, a method using 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) was developed for labeling vagal innervation of the gastrointestinal (GI) tract in embryonic and postnatal mice. DiI labeling was adapted ... More
Vagal afferent innervation of the atria of the rat heart reconstructed with confocal microscopy.
AuthorsCheng Z, Powley TL, Schwaber JS, Doyle FJ
JournalJ Comp Neurol
PubMed ID9087415
We have used confocal microscopy to analyze the vagal afferent innervation of the rat heart. Afferents were labeled by injecting 1,1'-dioleyl-3,3,3',3'-tetramethylindocarbocyanine methanesulfonate (DiI) into the nodose ganglia of animals with prior supranodose de-efferentations, autonomic ganglia were stained with Fluoro-gold, and tissues were examined in whole mounts. Distinctively different fiber specializations ... More