Growth-associated protein-43 is required for commissural axon guidance in the developing vertebrate nervous system.
AuthorsShen Y, Mani S, Donovan SL, Schwob JE, Meiri KF
JournalJ Neurosci
PubMed ID11756507
'Growth-associated protein-43 (GAP-43) is a major growth cone protein whose phosphorylation by PKC in response to extracellular guidance cues can regulate F-actin behavior. Here we show that 100% of homozygote GAP-43 (-/-) mice failed to form the anterior commissure (AC), hippocampal commissure (HC), and corpus callosum (CC) in vivo. Instead, ... More
Live cell imaging by multifocal multiphoton microscopy.
AuthorsStraub M, Lodemann P, Holroyd P, Jahn R, Hell SW
JournalEur J Cell Biol
PubMed ID11089921
Multifocal multiphoton microscopy (MMM) permits parallel multiphoton excitation by scanning an array of high numerical aperture foci across a plane in the sample. MMM is particularly suitable for live cell investigations since it combines advantages of standard multiphoton microscopy such as optical sectioning and suppression of out-of-focus phototoxicity with high ... More
The invagination of excess surface area by shrinking neurons.
AuthorsMorris CE, Wang JA, Markin VS
JournalBiophys J
PubMed ID12829478
Over most of their surface, neurons are surrounded by a narrow extracellular gap across which they make adhesive cell-cell contacts. Thus constrained, how do they regulate their geometry when osmotically perturbed? Specifically, are there any interesting consequences of local osmosis in such conditions? Using confocal imaging of shrinking neurons in ... More