Lipid rafts in the maintenance of synapses, dendritic spines, and surface AMPA receptor stability.
AuthorsHering H, Lin CC, Sheng M
JournalJ Neurosci
PubMed ID12716933
'Cholesterol/sphingolipid microdomains (lipid rafts) in the membrane are involved in protein trafficking, formation of signaling complexes, and regulation of actin cytoskeleton. Here, we show that lipid rafts exist abundantly in dendrites of cultured hippocampal neurons, in which they are associated with several postsynaptic proteins including surface AMPA receptors. Depletion of ... More
Fluorescence methods to detect phase boundaries in lipid bilayer mixtures.
'Phase diagrams of lipid mixtures can show several different regions of phase coexistence, which include liquid-disordered, liquid-ordered, and gel phases. Some phase regions are small, and some have sharp boundaries. The identity of the phases, their location in composition space, and the nature of the transitions between the phases are ... More
Carbocyanine dyes with long alkyl side-chains: broad spectrum inhibitors of mitochondrial electron transport chain activity.
AuthorsAnderson WM, Trgovcich-Zacok D
JournalBiochem Pharmacol
PubMed ID7763312
'Certain indocarbocyanine, thiacarbocyanine, and oxacarbocyanine dyes possessing short alkyl side-chains (one to five carbons) are potent inhibitors of mammalian mitochondrial NADH-ubiquinone reductase (EC 1.6.99.3) activity (Anderson et al., Biochem Pharmacol 41: 677-684, 1991; Anderson et al., Biochem Pharmacol 45: 691-696, 1993; Anderson et al., Biochem Pharmacol 45: 2115-2122, 1993), and ... More
Evidence for a role of dendritic filopodia in synaptogenesis and spine formation.
AuthorsZiv NE, Smith SJ
JournalNeuron
PubMed ID8755481
Axo-dendritic synaptogenesis was examined in live hippocampal cell cultures using the fluorescent dyes DiO to label dendrites and FM 4-64 to label functional presynaptic boutons. As the first functional synaptic boutons appeared in these cultures, numerous filopodia (up to 10 micron long) were observed to extend transiently (mean lifetime 9.5 ... More
A map of pheromone receptor activation in the mammalian brain.
AuthorsBelluscio L, Koentges G, Axel R, Dulac C
JournalCell
PubMed ID10219242
In mammals, the detection of pheromones is mediated by the vomeronasal system. We have employed gene targeting to visualize the pattern of projections of axons from vomeronasal sensory neurons in the accessory olfactory bulb. Neurons expressing a specific receptor project to multiple glomeruli that reside within spatially restricted domains. The ... More
Cell surface heparan sulfate proteoglycan syndecan-2 induces the maturation of dendritic spines in rat hippocampal neurons.
AuthorsEthell IM, Yamaguchi Y
JournalJ Cell Biol
PubMed ID9971750
Dendritic spines are small protrusions that receive synapses, and changes in spine morphology are thought to be the structural basis for learning and memory. We demonstrate that the cell surface heparan sulfate proteoglycan syndecan-2 plays a critical role in spine development. Syndecan-2 is concentrated at the synapses, specifically on the ... More
A novel method for simultaneous anterograde and retrograde labeling of spinal cord motor tracts in the same animal.
AuthorsTsai EC, van Bendegem RL, Hwang SW, Tator CH
JournalJ Histochem Cytochem
PubMed ID11511680
Examination of repaired spinal cord tracts has usually required separate groups of animals for anterograde and retrograde tracing owing to the incompatibility of techniques such as tissue fixation. However, anterograde and retrograde labeling of different animals subjected to the same repair may not allow accurate examination of that repair strategy ... More
Transbilayer peptide sorting between raft and nonraft bilayers: comparisons of detergent extraction and confocal microscopy.
AuthorsVidal A, McIntosh TJ
JournalBiophys J
PubMed ID15908585
Membrane microdomains ("rafts") that sequester specific proteins and lipids are often characterized by their resistance to detergent extraction. Because rafts are enriched in sphingomyelin and cholesterol, raft bilayers are thicker and have larger area compressibility moduli than nonraft bilayers. It has been postulated that rafts concentrate proteins with long transmembrane ... More
Exogenous expression of N-cadherin in breast cancer cells induces cell migration, invasion, and metastasis.
AuthorsHazan RB, Phillips GR, Qiao RF, Norton L, Aaronson SA
JournalJ Cell Biol
PubMed ID10684258
E- and N-cadherin are calcium-dependent cell adhesion molecules that mediate cell-cell adhesion and also modulate cell migration and tumor invasiveness. The loss of E-cadherin-mediated adhesion has been shown to play an important role in the transition of epithelial tumors from a benign to an invasive state. However, recent evidence indicates ... More
Endocytic sorting of lipid analogues differing solely in the chemistry of their hydrophobic tails.
AuthorsMukherjee S, Soe TT, Maxfield FR
JournalJ Cell Biol
PubMed ID10087269
To understand the mechanisms for endocytic sorting of lipids, we investigated the trafficking of three lipid-mimetic dialkylindocarbocyanine (DiI) derivatives, DiIC16(3) (1,1'-dihexadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate), DiIC12(3) (1,1'- didodecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate), and FAST DiI (1,1'-dilinoleyl-3,3,3', 3'-tetramethylindocarbocyanine perchlorate), in CHO cells by quantitative fluorescence microscopy. All three DiIs have the same head group, but differ in ... More
Flexible reprogramming of Pristionchus pacificus motivation for attacking Caenorhabditis elegans in predator-prey competition.
Authors
JournalCurr Biol
PubMed ID35259340
Packing Density of the Amyloid Precursor Protein in the Cell Membrane.
Authors
JournalBiophys J
PubMed ID29539399
Isolation of giant plasma membrane vesicles for evaluation of plasma membrane structure and protein partitioning.
Authors
JournalMethods Mol Biol
PubMed ID25331128
Compact ultrafast semiconductor disk laser: targeting GFP based nonlinear applications in living organisms.
Authors
JournalBiomed Opt Express
PubMed ID21483599
Astrocytic Ephrin-B1 Controls Synapse Formation in the Hippocampus During Learning and Memory.
Authors
JournalFront Synaptic Neurosci
PubMed ID32256333
Delineating the Rules for Structural Adaptation of Membrane-Associated Proteins to Evolutionary Changes in Membrane Lipidome.
Authors
JournalCurr Biol
PubMed ID31956022
Strip1 regulates retinal ganglion cell survival by suppressing Jun-mediated apoptosis to promote retinal neural circuit formation.