Functional analysis of FSP27 protein regions for lipid droplet localization, caspase-dependent apoptosis, and dimerization with CIDEA.
AuthorsLiu K, Zhou S, Kim JY, Tillison K, Majors D, Rearick D, Lee JH, Fernandez-Boyanapalli RF, Barricklow K, Houston MS, Smas CM,
JournalAm J Physiol Endocrinol Metab
PubMed ID19843876
'The adipocyte-specific protein FSP27, also known as CIDEC, is one of three cell death-inducing DFF45-like effector (CIDE) proteins. The first known function for CIDEs was promotion of apoptosis upon ectopic expression in mammalian cells. Recent studies in endogenous settings demonstrated key roles for CIDEs in energy metabolism. FSP27 is a ... More
Regulated localization of Rab18 to lipid droplets: effects of lipolytic stimulation and inhibition of lipid droplet catabolism.
AuthorsMartin S, Driessen K, Nixon SJ, Zerial M, Parton RG
JournalJ Biol Chem
PubMed ID16207721
'Rab GTPases are crucial regulators of membrane traffic. Here we have examined a possible association of Rab proteins with lipid droplets (LDs), neutral lipid-containing organelles surrounded by a phospholipid monolayer, also known as lipid bodies, which have been traditionally considered relatively inert storage organelles. Although we found close apposition between ... More
High glucose induces adipogenic differentiation of muscle-derived stem cells.
AuthorsAguiari P, Leo S, Zavan B, Vindigni V, Rimessi A, Bianchi K, Franzin C, Cortivo R, Rossato M, Vettor R, Abatangelo G, Pozzan T, Pinton P, Rizzuto R,
JournalProc Natl Acad Sci U S A
PubMed ID18212116
'Regeneration of mesenchymal tissues depends on a resident stem cell population, that in most cases remains elusive in terms of cellular identity and differentiation signals. We here show that primary cell cultures derived from adipose tissue or skeletal muscle differentiate into adipocytes when cultured in high glucose. High glucose induces ... More
Fixation methods for the study of lipid droplets by immunofluorescence microscopy.
AuthorsDiDonato D, Brasaemle DL
JournalJ Histochem Cytochem
PubMed ID12754288
'The study of proteins associated with lipid droplets in adipocytes and many other cells is a rapidly developing area of inquiry. Although lipid droplets are easily visible by light microscopy, few standardized microscopy methods have been developed. Several methods of chemical fixation have recently been used to preserve cell structure ... More
Spatial integration of TIP47 and adipophilin in macrophage lipid bodies.
AuthorsRobenek H, Lorkowski S, Schnoor M, Troyer D
JournalJ Biol Chem
PubMed ID15545278
'We studied the distribution of the PAT family proteins TIP47 and adipophilin in lipid bodies of THP-1 cell-derived macrophages using freeze-fracture immunolabeling and other techniques. Lipid bodies in macrophages comprise lipid droplets and extensive, previously scantily characterized sheet-like organelles, which we descriptively call "lipid sails." TIP47 and adipophilin are components ... More
Factors underlying the variability of lipid droplet fluorescence in MA-10 Leydig tumor cells.
AuthorsGocze PM, Freeman DA
JournalCytometry
PubMed ID7835165
'Neutral lipids accumulate in cellular lipid droplets. These droplets vary remarkably in number and amount between cells. In the present studies, the variability in lipid content was quantified by comparing the coefficient of variation of fluorescence histograms of nile red lipid-stained cells to the variability of cell size or cell ... More
Effects of HER2 overexpression on cell signaling networks governing proliferation and migration.
AuthorsWolf-Yadlin A, Kumar N, Zhang Y, Hautaniemi S, Zaman M, Kim HD, Grantcharova V, Lauffenburger DA, White FM,
JournalMol Syst Biol
PubMed ID17016520
'Although human epidermal growth factor receptor 2 (HER2) overexpression is implicated in tumor progression for a variety of cancer types, how it dysregulates signaling networks governing cell behavioral functions is poorly understood. To address this problem, we use quantitative mass spectrometry to analyze dynamic effects of HER2 overexpression on phosphotyrosine ... More
Fluorescent high-content imaging allows the discrimination and quantitation of E-LDL-induced lipid droplets and Ox-LDL-generated phospholipidosis in human macrophages.
AuthorsGrandl M, Schmitz G,
JournalCytometry A
PubMed ID20014301
'Macrophage foam cells formed during uptake of atherogenic lipoproteins are a hallmark of atherosclerotic lesion development. In this study, human macrophages were incubated with two prototypic atherogenic LDL modifications enzymatically degraded LDL (E-LDL) and oxidized LDL (Ox-LDL) prepared from the same donor LDL. To detect differences in macrophage lipid storage, ... More
Enhancement of BODIPY505/515 lipid fluorescence method for applications in biofuel-directed microalgae production.
AuthorsBrennan L, Blanco Fernández A, Mostaert AS, Owende P,
JournalJ Microbiol Methods
PubMed ID22521923
'This paper describes a microalgal cell lipid fluorescence enhancement method using BODIPY(505/515), which can be used to screen for lipids in wild-type microalgae and to monitor lipid content within microalgae production processes to determine optimal harvesting time. The study was based on four microalgae species (Dunaliella teteriolecta, Tetraselmis suecica, Nannochloropsis ... More
Increased lipid accumulation in the Chlamydomonas reinhardtii sta7-10 starchless isoamylase mutant and increased carbohydrate synthesis in complemented strains.
AuthorsWork VH, Radakovits R, Jinkerson RE, Meuser JE, Elliott LG, Vinyard DJ, Laurens LM, Dismukes GC, Posewitz MC,
JournalEukaryot Cell
PubMed ID20562225
'The accumulation of bioenergy carriers was assessed in two starchless mutants of Chlamydomonas reinhardtii (the sta6 [ADP-glucose pyrophosphorylase] and sta7-10 [isoamylase] mutants), a control strain (CC124), and two complemented strains of the sta7-10 mutant. The results indicate that the genetic blockage of starch synthesis in the sta6 and sta7-10 mutants ... More
Lateral diffusion of small compounds in human stratum corneum and model lipid bilayer systems.
AuthorsJohnson ME, Berk DA, Blankschtein D, Golan DE, Jain RK, Langer RS
JournalBiophys J
PubMed ID8913603
'An image-based technique of fluorescence recovery after photobleaching (video-FRAP) was used to measure the lateral diffusion coefficients of a series of nine fluorescent probes in two model lipid bilayer systems, dimyristoylphosphatidylcholine (DMPC) and DMPC/cholesterol (40 mol%), as well as in human stratum corneum-extracted lipids. The probes were all lipophilic, varied ... More
Esterified Cholesterol Is Highly Localized to Bruch's Membrane, as Revealed by Lipid Histochemistry in Wholemounts of Human Choroid.
AuthorsRudolf M, Curcio CA,
JournalJ Histochem Cytochem
PubMed ID19365091
'Accumulation of neutral lipids in Bruch''s membrane (BrM) is a major age change in human retina and contributes to the formation of extracellular lesions associated with age-related macular degeneration. We developed a BrM-choroid wholemounting technique suitable for reliable staining and evaluated different fluorescent lipid dyes for topographic semiquantitative analysis of ... More
Improved cytochemical method for detecting Jordans' bodies in neutral lipid storage diseases.
AuthorsTavian D, Colombo R
JournalJ Clin Pathol
PubMed ID17293389
Cytoplasmic lipid droplets are translocated into the lumen of the Chlamydia trachomatis parasitophorous vacuole.
AuthorsCocchiaro JL, Kumar Y, Fischer ER, Hackstadt T, Valdivia RH,
JournalProc Natl Acad Sci U S A
PubMed ID18591669
The acquisition of host-derived lipids is essential for the pathogenesis of the obligate intracellular bacteria Chlamydia trachomatis. Current models of chlamydial lipid acquisition center on the fusion of Golgi-derived exocytic vesicles and endosomal multivesicular bodies with the bacteria-containing parasitophorous vacuole ( ... More
The life of lipid droplets.
AuthorsWalther TC, Farese RV,
JournalBiochim Biophys Acta
PubMed ID19041421
Lipid droplets are the least characterized of cellular organelles. Long considered simple lipid storage depots, these dynamic and remarkable organelles have recently been implicated in many biological processes, and we are only now beginning to gain insights into their fascinating lives in cells. Here we examine what we know of ... More
Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae.
AuthorsPereira H, Barreira L, Mozes A, Florindo C, Polo C, Duarte CV, Custódio L, Varela J,
JournalBiotechnol Biofuels
PubMed ID22192119
We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto solid medium upon FACS can save about 3 weeks during the scale-up process as compared with the ... More
Comparative photostability studies of BODIPY and fluorescein dyes by using fluorescence correlation spectroscopy.
AuthorsHinkeldey B, Schmitt A, Jung G,
JournalChemphyschem
PubMed ID18816535
In single-molecule applications, the photostability of fluorescent molecules is a key parameter. We apply fluorescence correlation spectroscopy to compare the photostability of four fluorescein and four borondipyrromethene (BODIPY) dyes of similar structure but different triplet yields. The latter class of dyes are more stable. In the kinetic analysis the, diffusion ... More
Synthesis, chiroptical properties, and solid-state structure determination of two new chiral dipyrrin difluoroboryl chelates.
AuthorsGossauer A, Nydegger F, Kiss T, Sleziak R, Stoeckli-Evans H
JournalJ Am Chem Soc
PubMed ID14871109
Two new types of optically active BODIPY fluorophores bearing chiral phenyl substituents either at the meso-position or at both external alpha-positions have been synthesized. Their chiroptical properties are strongly dependent both on the position of the chiral group and on the protonation of the chromophore. The solid-state structures of one ... More
Leucine-rich repeat kinase 2 regulates autophagy through a calcium-dependent pathway involving NAADP.
Mutations in the leucine-rich repeat kinase-2 (LRRK2) gene cause late-onset Parkinson's disease, but its physiological function has remained largely unknown. Here we report that LRRK2 activates a calcium-dependent protein kinase kinase-ß (CaMKK-ß)/adenosine monophosphate (AMP)-activated protein kinase (AMPK) pathway which is followed by a persistent increase in autophagosome formation. Simultaneously, LRKR2 ... More
Evolutionarily conserved gene family important for fat storage.
AuthorsKadereit B, Kumar P, Wang WJ, Miranda D, Snapp EL, Severina N, Torregroza I, Evans T, Silver DL,
JournalProc Natl Acad Sci U S A
PubMed ID18160536
The ability to store fat in the form of cytoplasmic triglyceride droplets is conserved from Saccharomyces cerevisiae to humans. Although much is known regarding the composition and catabolism of lipid droplets, the molecular components necessary for the biogenesis of lipid droplets have remained obscure. Here we report the characterization of ... More
In situ analysis of heterogeneity in the lipid content of single green microalgae in alginate hydrogel microcapsules.
AuthorsLee DH, Bae CY, Han JI, Park JK,
Journal
PubMed ID24007509
Microalgae, a group of microorganisms that grow using sunlight as the sole energy source and carbon dioxide as an only carbon source, have been considered as a feedstock of choice for the production of biofuels such as biodiesel. To explore the economic feasibility of such application, however, many technical hurdles ... More
Pharmacological ER stress promotes hepatic lipogenesis and lipid droplet formation.
AuthorsLee JS, Mendez R, Heng HH, Yang ZQ, Zhang K,
JournalAm J Transl Res
PubMed ID22347525
Endoplasmic Reticulum (ER) stress refers to a condition of accumulation of unfolded or misfolded proteins in the ER lumen. A variety of biochemical stimuli or pathophysiologic conditions can directly or indirectly induce ER stress, leading to activation of an ER-originated adaptive signaling response called Unfolded Protein Response (UPR). Recent studies ... More
Altered lipid droplet dynamics in hepatocytes lacking triacylglycerol hydrolase expression.
AuthorsWang H, Wei E, Quiroga AD, Sun X, Touret N, Lehner R,
JournalMol Biol Cell
PubMed ID20410140
Lipid droplets (LDs) form from the endoplasmic reticulum (ER) and grow in size by obtaining triacylglycerols (TG). Triacylglycerol hydrolase (TGH), a lipase residing in the ER, is involved in the mobilization of TG stored in LDs for the secretion of very-low-density lipoproteins. In this study, we investigated TGH-mediated changes in ... More
Capillary electrochromatography and preconcentration of neutral compounds on poly(dimethylsiloxane) microchips.
AuthorsRo KW, Chang WJ, Kim H, Koo YM, Hahn JH
JournalElectrophoresis
PubMed ID14518053
Capillary electrochromatography (CEC) and preconcentration of neutral compounds have been realized on poly(dimethylsiloxane) (PDMS) microchips. The channels are coated with polyelectrolyte multilayers to avoid absorption of hydrophobic analytes into PDMS. The structures of a microchip include an injector and a bead chamber with integrated frits, where the particles of the ... More
ATGL has a key role in lipid droplet/adiposome degradation in mammalian cells.
AuthorsSmirnova E, Goldberg EB, Makarova KS, Lin L, Brown WJ, Jackson CL
JournalEMBO Rep
PubMed ID16239926
Lipid droplets (LDs), also called adiposomes, are found in many eukaryotic cells, and are highly upregulated in lipid-storage cells, such as adipocytes. The mechanism by which adiposomes and their component neutral lipids are degraded is an important health issue with the rapidly spreading epidemic of obesity. Recently, a novel triglyceride ... More
Oncogenic steroid receptor coactivator-3 is a key regulator of the white adipogenic program.
AuthorsLouet JF, Coste A, Amazit L, Tannour-Louet M, Wu RC, Tsai SY, Tsai MJ, Auwerx J, O'Malley BW
JournalProc Natl Acad Sci U S A
PubMed ID17098861
The white adipocyte is at the center of dysfunctional regulatory pathways in various pathophysiological processes, including obesity, diabetes, inflammation, and cancer. Here, we show that the oncogenic steroid receptor coactivator-3 (SRC-3) is a critical regulator of white adipocyte development. Indeed, in SRC-3(-/-) mouse embryonic fibroblasts, adipocyte differentiation was severely impaired, ... More
A duplexed microsphere-based fluorescent immunoassay.
AuthorsSzurdoki F, Michael KL, Walt DR
JournalAnal Biochem
PubMed ID11401295
Microsphere-based immunoassays are described for the simultaneous measurement of the clinically important drugs digoxin and theophylline. Competitive immunoassays were performed using haptenized microspheres and antibodies labeled with horseradish peroxidase. Enzyme-catalyzed reporter deposition (CARD) resulted in immunofluorescence signal amplification. Two encoding dyes were used to differentiate analytical signals from microspheres containing ... More
Rab18 localizes to lipid droplets and induces their close apposition to the endoplasmic reticulum-derived membrane.
AuthorsOzeki S, Cheng J, Tauchi-Sato K, Hatano N, Taniguchi H, Fujimoto T
JournalJ Cell Sci
PubMed ID15914536
Lipid droplets (LDs) are organelles that store neutral lipids, but their regulatory mechanism is not well understood. In the present study, we identified Rab18 as an LD component of HepG2 cells by proteomic analysis, and confirmed its localization by immunohistochemistry and western blotting. Wild-type and dominant-active Rab18 localized to LDs ... More
An integrated solid-phase extraction system for sub-picomolar detection.
AuthorsJemere AB, Oleschuk RD, Ouchen F, Fajuyigbe F, Harrison DJ
JournalElectrophoresis
PubMed ID12412122
A microchip structure etched on a glass substrate for packed column solid-phase extraction (SPE) and capillary electrochromatography (CEC) is described. A 200 microm long, octadecylsilane (ODS) packed column was secured using two different approaches: solvent lock or polymer entrapment. The former method was utilized for SPE while the latter approach ... More
Uptake and cellular localization of exogenous lipids by Giardia lamblia, a primitive eukaryote.
AuthorsStevens TL, Gibson GR, Adam R, Maier J, Allison-Ennis M, Das S
JournalExp Parasitol
PubMed ID9207743
Giardia lamblia trophozoites are unable to carry out de novo lipid synthesis. It is therefore likely that lipids are acquired from the small intestine of the host, in which the trophozoites are exposed to free and conjugated fatty acids, various sterols, phospholipids, bile acids, and bile-lipid mixed micelles. Here we ... More
Perilipin targets a novel pool of lipid droplets for lipolytic attack by hormone-sensitive lipase.
AuthorsMoore HP, Silver RB, Mottillo EP, Bernlohr DA, Granneman JG
JournalJ Biol Chem
PubMed ID16243839
Adipocytes serve as the principal energy reservoir of the body; however, the subcellular organization of the machinery regulating lipid trafficking and metabolism is poorly understood. Mobilization of stored triglyceride is thought be controlled by interactions among intracellular lipases and proteins that coat lipid storage droplets. A major limitation of previous ... More
The central domain is required to target and anchor perilipin A to lipid droplets.
AuthorsGarcia A, Sekowski A, Subramanian V, Brasaemle DL
JournalJ Biol Chem
PubMed ID12407111
The perilipins are the most abundant proteins coating the surfaces of lipid droplets in adipocytes and are found at lower levels surrounding lipid droplets in steroidogenic cells. Perilipins drive triacylglycerol storage in adipocytes by regulating the rate of basal lipolysis and are also required to maximize hormonally stimulated lipolysis. To ... More
Electrokinetic control of fluid flow in native poly(dimethylsiloxane) capillary electrophoresis devices.
AuthorsOcvirk G, Munroe M, Tang T, Oleschuk R, Westra K, Harrison DJ
JournalElectrophoresis
PubMed ID10634476
Capillary zone electrophoresis (CZE) devices fabricated in poly(dimethylsiloxane) (PDMS) require continuous voltage control of all intersecting channels in the fluidic network in order to avoid catastrophic leakage at the intersections. This contrasts with the behavior of similar flow channel designs fabricated in glass substrates. When the injection plugs are shaped ... More
Use of multi-photon laser-scanning microscopy to describe the distribution of xenobiotic chemicals in fish early life stages.
AuthorsHornung MW, Cook PM, Flynn KM, Lothenbach DB, Johnson RD, Nichols JW
JournalAquat Toxicol
PubMed ID15019246
To better understand the mechanisms by which persistent bioaccumulative toxicants (PBTs) produce toxicity during fish early life stages (ELS), dose-response relationships need to be understood in relation to the dynamic distribution of chemicals in sensitive tissues. In this study, a multi-photon laser scanning microscope (MPLSM) was used to determine the ... More
Perilipin A increases triacylglycerol storage by decreasing the rate of triacylglycerol hydrolysis.
The perilipins are the most abundant proteins at the surfaces of lipid droplets in adipocytes and are also found in steroidogenic cells. To investigate perilipin function, perilipin A, the predominant isoform, was ectopically expressed in fibroblastic 3T3-L1 pre-adipocytes that normally lack the perilipins. In control cells, fluorescent staining of neutral ... More
The phosphorylation of serine 492 of perilipin a directs lipid droplet fragmentation and dispersion.
AuthorsMarcinkiewicz A, Gauthier D, Garcia A, Brasaemle DL
JournalJ Biol Chem
PubMed ID16488886
Perilipin A is a key regulator of triacylglycerol storage and hydrolysis in adipocytes; phosphorylation of perilipin A by protein kinase A facilitates maximal lipolysis. Chronic stimulation of lipolysis in 3T3-L1 adipocytes causes large perinuclear lipid droplets to fragment into myriad dispersed perilipin A-covered microlipid droplets. In cultured fibroblasts stably expressing ... More
Age-related ultrastructural changes of the basement membrane in the mouse blood-brain barrier.
AuthorsCeafalan LC, Fertig TE, Gheorghe TC, Hinescu ME, Popescu BO, Pahnke J, Gherghiceanu M
JournalJ Cell Mol Med
PubMed ID30450815
'The blood-brain barrier (BBB) is essential for a functional neurovascular unit. Most studies focused on the cells forming the BBB, but very few studied the basement membrane (BM) of brain capillaries in ageing. We used transmission electron microscopy and electron tomography to investigate the BM of the BBB in ageing ... More
Atheroprotective roles of smooth muscle cell phenotypic modulation and the TCF21 disease gene as revealed by single-cell analysis.
Authors
JournalNat Med
PubMed ID31359001
TGFß2-induced formation of lipid droplets supports acidosis-driven EMT and the metastatic spreading of cancer cells.
AuthorsCorbet C, Bastien E, Santiago de Jesus JP, Dierge E, Martherus R, Vander Linden C, Doix B, Degavre C, Guilbaud C, Petit L, Michiels C, Dessy C, Larondelle Y, Feron O
JournalNat Commun
PubMed ID31974393
'Acidosis, a common characteristic of the tumor microenvironment, is associated with alterations in metabolic preferences of cancer cells and progression of the disease. Here we identify the TGF-ß2 isoform at the interface between these observations. We document that acidic pH promotes autocrine TGF-ß2 signaling, which in turn favors the formation of ... More
Inhibition of TGF-ß induced lipid droplets switches M2 macrophages to M1 phenotype.
AuthorsBose D, Banerjee S, Chatterjee N, Das S, Saha M, Saha KD
JournalToxicol In Vitro
PubMed ID30930231
'Lipid droplets (LD) are newly characterized dynamic cytoplasmic organelle which is the storehouse of different immunosuppressive cytokines and enzymes like cyclooxygenase and lipoxygenase. Tumors are known to modulate the immune system by immune-editing the microenvironment. Immuno-editing comprises of three steps namely cancer immune-surveillance, tumor dormancy and finally escape leading to ... More
Gold Nanoparticle Transforms Activated Cancer-Associated Fibroblasts to Quiescence.
AuthorsHossen MN, Rao G, Dey A, Robertson JD, Bhattacharya R, Mukherjee P
JournalACS Appl Mater Interfaces
PubMed ID31117437
'Activated cancer-associated fibroblasts (CAFs) play a major role in the poor outcome in many diseases including pancreatic cancer. Normally quiescent with high lipid content and low proliferative capacity, CAFs receiving cues from cancer cells in the tumor microenvironment become activated and transformed into a lipid-deprived and highly proliferative myofibroblast type ... More
The Cytokine TNF Promotes Transcription Factor SREBP Activity and Binding to Inflammatory Genes to Activate Macrophages and Limit Tissue Repair.
Authors
JournalImmunity
PubMed ID31303399
TLE3 is a dual-function transcriptional coregulator of adipogenesis.
Authors
JournalCell Metab
PubMed ID21459326
The Inner Nuclear Membrane Is a Metabolically Active Territory that Generates Nuclear Lipid Droplets.
An immune-beige adipocyte communication via nicotinic acetylcholine receptor signaling.
Authors
JournalNat Med
PubMed ID29785025
Treg Cells Promote the SREBP1-Dependent Metabolic Fitness of Tumor-Promoting Macrophages via Repression of CD8+ T Cell-Derived Interferon-γ.
Authors
JournalImmunity
PubMed ID31350177
Succinyl-CoA Ligase Deficiency in Pro-inflammatory and Tissue-Invasive T Cells.
Authors
JournalCell Metab
PubMed ID33264602
Targeting DGAT1 Ameliorates Glioblastoma by Increasing Fat Catabolism and Oxidative Stress.
Authors
JournalCell Metab
PubMed ID32559414
Local Mitochondrial ATP Production Regulates Endothelial Fatty Acid Uptake and Transport.
Authors
JournalCell Metab
PubMed ID32521232
Tumor-Derived Ligands Trigger Tumor Growth and Host Wasting via Differential MEK Activation.
AuthorsSong W, Kir S, Hong S, Hu Y, Wang X, Binari R, Tang HW, Chung V, Banks AS, Spiegelman B, Perrimon N
JournalDev Cell
PubMed ID30639055
Interactions between tumors and host tissues play essential roles in tumor-induced systemic wasting and cancer cachexia, including muscle wasting and lipid loss. However, the pathogenic molecular mechanisms of wasting are still poorly understood. Using a fly model of tumor-induced organ wasting, we observed aberrant MEK activation in both tumors and ... More
Lipid accumulation impairs natural killer cell cytotoxicity and tumor control in the postoperative period.
AuthorsNiavarani SR, Lawson C, Bakos O, Boudaud M, Batenchuk C, Rouleau S, Tai LH
JournalBMC Cancer
PubMed ID31429730
Natural killer (NK) cell dysfunction following cancer surgery has been shown to promote metastases. Recent studies demonstrate an emerging role for lipids in the modulation of NK cell innate responses. However, the mechanisms involved in lipid modulation of NK cell postoperative anti-tumor function are unknown. This current study will determine ... More
Clockophagy is a novel selective autophagy process favoring ferroptosis.
AuthorsYang M, Chen P, Liu J, Zhu S, Kroemer G, Klionsky DJ, Lotze MT, Zeh HJ, Kang R, Tang D
JournalSci Adv
PubMed ID31355331
Ferroptosis is a form of nonapoptotic regulated cell death driven by iron-dependent lipid peroxidation. Autophagy involves a lysosomal degradation pathway that can either promote or impede cell death. A high level of autophagy has been associated with ferroptosis, but the mechanisms underpinning this relationship are largely elusive. We characterize the ... More
Lipid droplet velocity is a microenvironmental sensor of aggressive tumors regulated by V-ATPase and PEDF.
AuthorsNardi F, Fitchev P, Brooks KM, Franco OE, Cheng K, Hayward SW, Welte MA, Crawford SE
JournalLab Invest
PubMed ID31409893
Lipid droplets (LDs) utilize microtubules (MTs) to participate in intracellular trafficking of cargo proteins. Cancer cells accumulate LDs and acidify their tumor microenvironment (TME) by increasing the proton pump V-ATPase. However, it is not known whether these two metabolic changes are mechanistically related or influence LD movement. We postulated that ... More