Nitric oxide protects cultured rat hepatocytes from tumor necrosis factor-alpha-induced apoptosis by inducing heat shock protein 70 expression.
AuthorsKim YM,de Vera ME,Watkins SC,Billiar TR
JournalThe Journal of biological chemistry
PubMed ID8995451
Differing effects of copper,zinc superoxide dismutase overexpression on neurotoxicity elicited by nitric oxide, reactive oxygen species, and excitotoxins.
AuthorsYing W,Anderson CM,Chen Y,Stein BA,Fahlman CS,Copin JC,Chan PH,Swanson RA
JournalJournal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
PubMed ID10698074
Authors
Journal
PubMed ID11282963
Imaging of hydroperoxides in a rat glomerulus stimulated by puromycin aminonucleoside.
Cellular alterations produced by the experimental increase in intracellular calcium and the nature of protective effects from pretreatment with nimodipine.
The immortalized septal cell line, SN56 B5 G4, generated by the fusion of mouse septal area cells and neuroblastoma cells, was used to determine if nimodipine, an antagonist of voltage sensitive calcium 'L' channels, might act in a neuroprotective fashion when intracellular calcium levels were raised by incubation in ouabain ... More
Alcohol-induced thymocyte apoptosis is accompanied by impaired mitochondrial function.
AuthorsWang JF,Spitzer JJ
JournalAlcohol (Fayetteville, N.Y.)
PubMed ID9014030
This study examines the effects of chronic alcohol consumption on thymic apoptosis with or without pretreatment with E. coli lipopolysaccharide (LPS). Apoptotic cell death of thymocytes was monitored by DNA fragments in gel electrophoresis and the appearance of apoptotic cells by flow cytometry. Changes in mitochondrial membrane potential (MMP), as ... More
Oxygen-induced embryopathy and the significance of glutathione-dependent antioxidant system in the rat embryo during early organogenesis.
We investigated the effect of glutathione (GSH)-dependent antioxidant system against hydrogen peroxide (H2O2) formation in oxygen-induced embryopathy. Exposure of rat embryos to a high concentration of oxygen (20%) during early neurulation (day 9 to 10) significantly increased the incidence of neural tube defects compared with control embryos (10% vs 0%, ... More
Complex N-glycan and metabolic control in tumor cells.
AuthorsMendelsohn R, Cheung P, Berger L, Partridge E, Lau K, Datti A, Pawling J, Dennis JW,
JournalCancer Res
PubMed ID17942907
Golgi beta1,6N-acetylglucosaminyltransferase V (Mgat5) produces beta1,6GlcNAc-branched complex N-glycans on cell surface glycoproteins that bind to galectins and promote surface residency of glycoproteins, including cytokine receptors. Carcinoma cells from polyomavirus middle T (PyMT) transgenic mice on a Mgat5-/- background have reduced surface levels of epidermal growth factor (EGF) and transforming growth ... More
Mammalian peroxiredoxin isoforms can reduce hydrogen peroxide generated in response to growth factors and tumor necrosis factor-alpha.
AuthorsKang SW, Chae HZ, Seo MS, Kim K, Baines IC, Rhee SG
JournalJ Biol Chem
PubMed ID9497357
Mammalian tissues express three immunologically distinct peroxiredoxin (Prx) proteins (Prx I, II, and III), which are the products of distinct genes. With the use of recombinant proteins Prx I, II, and III, all have now been shown to possess peroxidase activity and to rely on Trx as a source of ... More
Regulation of the human ether-a-gogo related gene (HERG) K+ channels by reactive oxygen species.
AuthorsTaglialatela M, Castaldo P, Iossa S, Pannaccione A, Fresi A, Ficker E, Annunziato L
JournalProc Natl Acad Sci U S A
PubMed ID9326673
Human ether-a-gogo related gene (HERG) K+ channels are key elements in the control of cell excitability in both the cardiovascular and the central nervous systems. For this reason, the possible modulation by reactive oxygen species (ROS) of HERG and other cloned K+ channels expressed in Xenopus oocytes has been explored ... More
Reactive oxygen species and calcium homeostasis in cultured human intestinal smooth muscle cells.
AuthorsBielefeldt K, Whiteis CA, Sharma RV, Abboud FM, Conklin JL
JournalAm J Physiol
PubMed ID9227480
Reactive oxygen species (ROS) significantly alter cell function. We examined the effects of hydrogen peroxide (H2O2) and xanthine/xanthine oxidase (X/XO) on isolated intestinal muscle cells. We assessed cell viability with the exclusion dye trypan blue and assayed the effects of H2O2 and X/XO on the intracellular redox state with the ... More
Standardization of a flow cytometric assay for phagocyte respiratory burst activity.
AuthorsVuorte J, Jansson SE, Repo H
JournalScand J Immunol
PubMed ID8602468
The authors optimized the flow cytometric dichlorofluorescin (DCFH)-oxidation assay for buffy coat neutrophil and monocyte respiratory burst activity. Sample handlings were minimized, monocytes identified with a CD14 antibody, and viability evaluated with propidium iodide. Sodium citrate was a better anticoagulant than heparin, with a more intense Yersinia enterocolitica (YER)-induced dichlorofluorescein ... More
AuthorsSchwarz MA, Lazo JS, Yalowich JC, Reynolds I, Kagan VE, Tyurin V, Kim YM, Watkins SC, Pitt BR
JournalJ Biol Chem
PubMed ID8195159
Metallothioneins (MT) are ubiquitous low molecular weight metal-binding proteins that may act as antioxidants. We examined the sensitivity of NIH 3T3 cells transfected with a plasmid containing mouse metallothionein-I gene (NIH3T3/MT) to the membrane permeant oxidant, tert-butyl hydroperoxide (tBH). NIH3T3/MT cells had a 4-fold increase in intracellular metallothionein as compared ... More
Molecular mechanisms of nickel carcinogenesis.
AuthorsCosta M, Salnikow K, Cosentino S, Klein CB, Huang X, Zhuang Z
JournalEnviron Health Perspect
PubMed ID7843086
Carcinogenic, water-insoluble Ni compounds are phagocytized by cells; and the particles undergo dissolution inside the cell, releasing Ni ions that interact with chromatin. Ni produces highly selective damage to heterochromatin. The longest contiguous region of heterochromatin in the Chinese hamster genome is found on the q arm of the X ... More
Molecular mechanisms of nickel carcinogenesis.
AuthorsCosta M, Zhuang Z, Huang X, Cosentino S, Klein CB, Salnikow K
JournalSci Total Environ
PubMed ID8029695
Nickel treatment of intact cultured cells oxidized dichlorofluorescin to a fluorescent product indicating that nickel elevated the level of oxidants in cells. Nickel also caused an increase in crosslinking of amino acids to DNA and these complexes did not appear to involve the direct participation of Ni2+. Histidine, cysteine and ... More
Production of hydrogen peroxide by murine epidermal keratinocytes following treatment with the tumor promoter 12-O-tetradecanoylphorbol-13-acetate.
The ability of murine epidermal cells to produce intracellular hydrogen peroxide was analyzed by flow cytometry and the measurement of 2',7'- dichlorofluorescin (DCFH) oxidation. Epidermal cells isolated from acetone-treated CD-1 mice for 24 h were relatively homogeneous in cell size and density and oxidized low levels of DCFH. However, following ... More
A microtiter plate assay for screening antioxidant activity in extracts of marine organisms.
AuthorsDunlap W, Llewellyn L, Doyle J, Yamamoto Y
JournalMar Biotechnol (NY)
PubMed ID14502401
A novel microtiter plate assay was developed to determine the total peroxyl radical-trapping activity of antioxidants extracted from marine organisms by measuring the inhibition rate of dye-substrate oxidation. We compared use of dihydrorhodamine-123, dihydrofluorescein, and dichlorodihydrofluorescein as reduced substrates for oxidation by peroxyl radicals generated from 2,2'-azobis(2-amidinopropane) dihydrochloride. The oxidation ... More
Up-regulation of aldose reductase by the substrate, methylglyoxal.
AuthorsYabe-Nishimura C, Nishinaka T, Iwata K, Seo HG
JournalChem Biol Interact
PubMed ID12604218
Methylglyoxal (MG), a reactive dicarbonyl produced during glucose metabolism, is known as a preferred substrate of aldose reductase (AR; AKR1B1) that concomitantly catalyzes the reduction of glucose in the polyol pathway. MG modifies cellular proteins to form cross-links of amino groups, generating so-called advanced glycation end products. Increased rates of ... More
Effects of heat shock on in vitro development and intracellular oxidative state of bovine preimplantation embryos.
AuthorsSakatani M, Kobayashi S, Takahashi M
JournalMol Reprod Dev
PubMed ID14648877
We investigated the effects of heat shock on developmental competence of bovine embryos and intracellular oxidative state. After in vitro fertilization, embryos were exposed to heat shock at 41 degrees C for 6 hr on days 0, 2, 4, and 6, respectively. On day 2, cleavage rate was not significantly ... More
Synaptic mitochondria are more susceptible to Ca2+overload than nonsynaptic mitochondria.
AuthorsBrown MR, Sullivan PG, Geddes JW
JournalJ Biol Chem
PubMed ID16517608
'Mitochondria in nerve terminals are subjected to extensive Ca2+ fluxes and high energy demands, but the extent to which the synaptic mitochondria buffer Ca2+ is unclear. In this study, we identified a difference in the Ca2+ clearance ability of nonsynaptic versus synaptic mitochondrial populations enriched from rat cerebral cortex. Mitochondria ... More
Titrating the effects of mitochondrial complex I impairment in the cell physiology.
AuthorsBarrientos A, Moraes CT
JournalJ Biol Chem
PubMed ID10347173
'The mitochondrial oxidative phosphorylation system consists of five multimeric enzymes (complexes I-V). NADH dehydrogenase or complex I (CI) is affected in most of the mitochondrial diseases and in some neurodegenerative disorders. We have studied the physiological consequences of a partial CI inhibition at the cellular level. We used a genetic ... More
Cytokine-induced activation of nuclear factor-kappa B is inhibited by hydrogen peroxide through oxidative inactivation of IkappaB kinase.
AuthorsKorn SH, Wouters EF, Vos N, Janssen-Heininger YM
JournalJ Biol Chem
PubMed ID11479295
'Rapid activation of the IkappaB kinase (IKK) complex is considered an obligatory step in the activation of nuclear factor-kappaB (NF-kappaB) in response to diverse stimuli. Since oxidants have been implicated in the regulation of NF-kappaB, the focus of the present study was the activation of IKK by tumor necrosis factor ... More
Inhibition of wild-type p66ShcA in mesangial cells prevents glycooxidant-dependent FOXO3a regulation and promotes the survival phenotype.
AuthorsChintapalli J, Yang S, Opawumi D, Goyal SR, Shamsuddin N, Malhotra A, Reiss K, Meggs LG,
JournalAm J Physiol Renal Physiol
PubMed ID17077388
'Hyperglycemia triggers an exponential increase in reactive oxygen species (ROS) at the cellular level. Here, we demonstrate induction of the oxidant-resistant phenotype in mesangial cells by silencing the wild-type (WT) p66ShcA gene. Two approaches were employed to inhibit WTp66ShcA in SV40 murine mesangial cells and normal human mesangial cells: transient ... More
Reactive oxygen species as essential mediators of cell adhesion: the oxidative inhibition of a FAK tyrosine phosphatase is required for cell adhesion.
AuthorsChiarugi P, Pani G, Giannoni E, Taddei L, Colavitti R, Raugei G, Symons M, Borrello S, Galeotti T, Ramponi G
JournalJ Cell Biol
PubMed ID12796479
'Signal transduction by reactive oxygen species (ROS; "redox signaling") has recently come into focus in cellular biology studies. The signaling properties of ROS are largely due to the reversible oxidation of redox-sensitive target proteins, and especially of protein tyrosine phosphatases, whose activity is dependent on the redox state of a ... More
CFTR regulates phagosome acidification in macrophages and alters bactericidal activity.
AuthorsDi A, Brown ME, Deriy LV, Li C, Szeto FL, Chen Y, Huang P, Tong J, Naren AP, Bindokas V, Palfrey HC, Nelson DJ
JournalNat Cell Biol
PubMed ID16921366
'Acidification of phagosomes has been proposed to have a key role in the microbicidal function of phagocytes. Here, we show that in alveolar macrophages the cystic fibrosis transmembrane conductance regulator Cl- channel (CFTR) participates in phagosomal pH control and has bacterial killing capacity. Alveolar macrophages from Cftr-/- mice retained the ... More
Altered production of nitric oxide and reactive oxygen species in rat nodose ganglion neurons during acute hypoxia.
AuthorsYamamoto Y, Henrich M, Snipes RL, Kummer W
JournalBrain Res
PubMed ID12535770
'Nitric oxide (NO) production in the sensory neurons of the rad nodose ganglion was studied by examining the distribuiotn of NO synthase (NOS) by use of NADPH diaphorase (NADPHD) histochemistry and immunohistochemistry ofr the presence of isoformes of NOS: neuronal (nNOS), endothelial (eNOS) and the inducible isoform (iNOS). Distribution and ... More
Induction of vascular endothelial growth factor expression and hypoxia-inducible factor 1alpha protein by the oxidative stressor arsenite.
AuthorsDuyndam MC, Hulscher TM, Fontijn D, Pinedo HM, Boven E
JournalJ Biol Chem
PubMed ID11641398
'Recent evidence suggests that vascular endothelial growth factor (VEGF) expression is up-regulated by oxidative stressors through activation of hypoxia-inducible Factor 1 (HIF-1). To investigate whether this is a general phenomenon, we studied the effects of the sulfhydryl reagent arsenite on VEGF expression in human ovarian cancer cells. Arsenite potently induces ... More
Cyclopentenone prostaglandins as potential inducers of intracellular oxidative stress.
AuthorsKondo M, Oya-Ito T, Kumagai T, Osawa T, Uchida K
JournalJ Biol Chem
PubMed ID11278531
'In the present study, we find that cyclopentenone prostaglandins (PGs) of the J(2) series, naturally occurring derivatives of PGD(2), are potential inducers of intracellular oxidative stress that mediates cell degeneration. Based on an extensive screening of diverse chemical agents on induction of intracellular production of reactive oxygen species (ROS), we ... More
Quantification of Bax and Bcl2 in polymorphonuclear leukocytes from haemodialysis patients: relation to hydrogen peroxide.
'BACKGROUND: Bax and Bcl2 are two apoptosis-related molecules that play an important role in determining cell fate following oxidative injury. In the present study, we explored the relation of hydrogen peroxide (H2O2) generation by polymorphonuclear cells (PMNs) to the cytosolic expression of Bax and Bcl2 proteins and apoptosis in haemodialysis ... More
Trafficking of ganglioside GD3 to mitochondria by tumor necrosis factor-alpha.
AuthorsGarcía-Ruiz C, Colell A, Morales A, Calvo M, Enrich C, Fernández-Checa JC
JournalJ Biol Chem
PubMed ID12118012
'The interaction of mitochondria with proapoptotic proteins activates apoptosis pathways. Previous findings have identified ganglioside GD3 (GD3) as an emerging apoptotic lipid intermediate that targets mitochondria in response to death signals. Using immunoelectron and laser scanning confocal microscopy, we characterize the trafficking of GD3 to mitochondria in response to tumor ... More
Two-photon fluorescence absorption and emission spectra of dyes relevant for cell imaging.
AuthorsBestvater F, Spiess E, Stobrawa G, Hacker M, Feurer T, Porwol T, Berchner-Pfannschmidt U, Wotzlaw C, Acker H
JournalJ Microsc
PubMed ID12423261
'Two-photon absorption and emission spectra for fluorophores relevant in cell imaging were measured using a 45 fs Ti:sapphire laser, a continuously tuneable optical parametric amplifier for the excitation range 580-1150 nm and an optical multichannel analyser. The measurements included DNA stains, fluorescent dyes coupled to antibodies as well as organelle ... More
Secreted beta-amyloid precursor protein counteracts the proapoptotic action of mutant presenilin-1 by activation of NF-kappaB and stabilization of calcium homeostasis.
AuthorsGuo Q, Robinson N, Mattson MP
JournalJ Biol Chem
PubMed ID9575187
'Mutations in the presenilin-1 (PS-1) gene account for approximately 50% of the cases of autosomal dominant, early onset, inherited forms of Alzheimer's disease (AD). PS-1 is an integral membrane protein expressed in neurons and is localized primarily in the endoplasmic reticulum (ER). PS-1 mutations may promote neuronal degeneration by altering ... More
Chronic hypoxia protects against gamma-irradiation-induced apoptosis by inducing bcl-2 up-regulation and inhibiting mitochondrial translocation and conformational change of bax protein.
AuthorsCuisnier O, Serduc R, Lavieille JP, Longuet M, Reyt E, Riva C
JournalInt J Oncol
PubMed ID12963983
'Malignant tumours contain zones of chronic or acute hypoxia, which influence their prognosis and progression. The goal of our study was to understand the role of hypoxia in radio-resistance in a squamous cell carcinoma cell line of the head and neck (KB-3-1 cells). Cell growth was evaluated by Trypan blue ... More
p53-induced up-regulation of MnSOD and GPx but not catalase increases oxidative stress and apoptosis.
AuthorsHussain SP, Amstad P, He P, Robles A, Lupold S, Kaneko I, Ichimiya M, Sengupta S, Mechanic L, Okamura S, Hofseth LJ, Moake M, Nagashima M, Forrester KS, Harris CC
JournalCancer Res
PubMed ID15059885
'p53-mediated apoptosis may involve the induction of redox-controlling genes, resulting in the production of reactive oxygen species. Microarray expression analysis of doxorubicin exposed, related human lymphoblasts, p53 wild-type (WT) Tk6, and p53 mutant WTK1 identified the p53-dependent up-regulation of manganese superoxide dismutase (MnSOD) and glutathione peroxidase 1 (GPx). Consensus p53 ... More
Early induction and late abrogation of respiratory burst in A. phagocytophilum-infected neutrophils.
AuthorsChoi KS, Dumler JS
JournalAnn N Y Acad Sci
PubMed ID12860678
'Human granulocytic ehrlichiosis is an emerging tick-borne disease caused by the obligate intracellular pathogen, Anaplasma phagocytophilum. This organism is unique because it survives and propagates in neutrophil vacuoles. During phagocytosis professional phagocytes increase oxygen consumption (respiratory burst) through the activity of NADPH-oxidase, which generates superoxide anion (O2(-)) and hydrogen peroxide ... More
Functional defects in phagocytic cells following thermal injury. Application of flow cytometric analysis.
AuthorsDuque RE, Phan SH, Hudson JL, Till GO, Ward PA
JournalAm J Pathol
PubMed ID2981471
'Defective phagocytic cell function may partially account for the morbidity and mortality associated with thermal injury. In experimental thermal injury in the rat, small circulating blood volumes increase the difficulty in obtaining significant data. Furthermore, purification and or elicitation procedures have the potential for altering the cell surface characteristics and/or ... More
Effect of chlorophyllin against oxidative stress in splenic lymphocytes in vitro and in vivo.
AuthorsKumar SS, Shankar B, Sainis KB
JournalBiochim Biophys Acta
PubMed ID15110092
'Chlorophyllin (CHL) has been examined as an antioxidant/radioprotector in splenic lymphocytes from BALB/c mice. CHL inhibited lipid peroxidation induced by 2,2''-azobis(2-propionimidinedihydrochloride) (AAPH) in lymphocytes in vitro. It also partially prevented radiation-induced suppression of mitogenic stimulation of lymphocytes in vitro. Generation of intracellular reactive oxygen species (ROS) by radiation or AAPH ... More
Free radical generation and oxidative stress with ageing and exercise: differential effects in the myocardium and liver.
AuthorsBejma J, Ramires P, Ji LL
JournalActa Physiol Scand
PubMed ID10951126
'Reactive oxygen species and other oxidants are implicated in the mechanisms of biological ageing and exercise-induced tissue damage. The present study examined the effects of ageing and an acute bout of exercise on intracellular oxidant generation, lipid peroxidation, protein oxidation and glutathione (GSH) status in the heart and liver of ... More
CD18/ICAM-1-dependent oxidative NF-kappaB activation leading to nitric oxide production in rat Kupffer cells cocultured with syngeneic hepatoma cells.
AuthorsKurose I, Saito H, Miura S, Ebinuma H, Higuchi H, Watanabe N, Zeki S, Nakamura T, Takaishi M, Ishii H
JournalJ Clin Invest
PubMed ID9062344
'Previous studies have indicated that nitric oxide (NO) released from Kupffer cells modulates biological viability of cocultured hepatoma cells. This study was designed to evaluate the mechanisms by which Kupffer cells synthesize and release NO in reponse to cocultured hepatoma cells. Kupffer cells isolated from male Wistar rats were cocultured ... More
Effects of glutathione and pH on the oxidation of biomarkers of cellular oxidative stress.
AuthorsZhu H, He M, Bannenberg GL, Moldéus P, Shertzer HG
JournalArch Toxicol
PubMed ID8870955
'Cellular oxidative stress is associated with such pathological conditions as arteriosclerosis, inflammatory diseases and cancer. The oxidation of the biomarkers. 2'',7''-dichlorofluorescin (DCFH), 2-deoxyribose, and lipid peroxidation are often used to assess the status of oxidative stress in cells and tissues. Since high levels of reduced glutathione (GSH) and acidic conditions ... More
Leptin stimulates the oxidative burst in control monocytes but attenuates the oxidative burst in monocytes from HIV-infected patients.
AuthorsSánchez-Pozo C, Rodriguez-Baño J, Domínguez-Castellano A, Muniain MA, Goberna R, Sánchez-Margalet V
JournalClin Exp Immunol
PubMed ID14632752
'Leptin, the 16 kDa product of the ob gene, is a an adipocyte-secreted hormone that centrally regulates weight. However, the physiological role of leptin is not limited to the regulation of food intake and energy expenditure, and leptin has a variety of effects in peripheral tissues, such as a regulatory ... More
Sodium nitroprusside prevents chemical hypoxia-induced cell death through iron ions stimulating the activity of the Na+-Ca2+ exchanger in C6 glioma cells.
AuthorsAmoroso S, Tortiglione A, Secondo A, Catalano A, Montagnani S, Di Renzo G, Annunziato L
JournalJ Neurochem
PubMed ID10737607
'In C6 glioma cells exposed to chemical hypoxia, an increase of extracellular lactate dehydrogenase (LDH) activity, cell death, and intracellular Ca2+ concentration ([Ca2+]i) occurred. Sodium nitroprusside (SNP), a nitric oxide donor and an iron-containing molecule, reduced chemical hypoxia-induced LDH release and cell death. These effects were counteracted by bepridil and ... More
Nitric oxide generation and poly(ADP ribose) polymerase activation precede beta-cell death in rats with a single high-dose injection of streptozotocin.
AuthorsWada R, Yagihashi S
JournalVirchows Arch
PubMed ID14762714
'Streptozotocin (STZ) is widely used for the induction of diabetes in animals by causing destruction of pancreatic beta cells. This experiment was designed to elucidate the sequential process of beta-cell destruction in rats with a single high-dose injection of STZ. At 0, 2, 5, 8 and 24 h after injection, ... More
Effects of oxidants and glutamate receptor activation on mitochondrial membrane potential in rat forebrain neurons.
AuthorsScanlon JM, Reynolds IJ
JournalJ Neurochem
PubMed ID9832137
'Both glutamate and reactive oxygen species have been implicated in excitotoxic neuronal injury, and mitochondria may play a key role in the mediation of this process. In this study, we examined whether glutamate-receptor stimulation and oxidative stress interact to affect the mitochondrial membrane potential (delta psi). We measured delta psi ... More
Reactive oxygen species (ROS)-induced ROS release: a new phenomenon accompanying induction of the mitochondrial permeability transition in cardiac myocytes.
'We sought to understand the relationship between reactive oxygen species (ROS) and the mitochondrial permeability transition (MPT) in cardiac myocytes based on the observation of increased ROS production at sites of spontaneously deenergized mitochondria. We devised a new model enabling incremental ROS accumulation in individual mitochondria in isolated cardiac myocytes ... More
Oxidative stress and mitochondrial glutathione in human lymphocytes exposed to clinically relevant anesthetic drug concentrations.
AuthorsDelogu G, Antonucci A, Moretti S, Marandola M, Tellan G, Signore M, Famularo G
JournalJ Clin Anesth
PubMed ID15217658
'STUDY OBJECTIVE: To evaluate the potential of compounds commonly used in anesthesia practice to affect the intracellular oxidant-antioxidant homeostasis of peripheral blood lymphocytes at clinically relevant concentrations; and to study the changes in reactive oxygen species production and measure the mitochondrial glutathione content. DESIGN: Prospective, in vitro study. SETTING: Experimental ... More
Indirect detection of photosensitizer ex vivo.
AuthorsBourré L, Thibaut S, Briffaud A, Rousset N, Eléouet S, Lajat Y, Patrice T
JournalJ Photochem Photobiol B
PubMed ID12007464
'Photodynamic therapy induces the production of reactive oxygen species (ROS) within tissues exposed to laser light after administration of a sensitizer. In the context of continuing clinical and commercial development of chemicals with sensitizing properties, a minimally invasive assay is needed to determine the tissue kinetics of fluorescent or non-fluorescent ... More
Subtoxic concentration of manganese synergistically potentiates 1-methyl-4-phenylpyridinium-induced neurotoxicity in PC12 cells.
AuthorsWang RG, Zhu XZ
JournalBrain Res
PubMed ID12535785
'Endogenous or exogenous substances that are toxic to dopaminergic cells have been proposed as possible cause of idiopathic Parkinson''s disease (PD). 1-Methyl-4-phenylpyridinium (MPP(+)) and manganese are dopaminergic neurotoxins causing a parkinsonism-like syndrome. Here, we studied the possible synergistic reaction between these two neurotoxins using rat PC12 pheochromocytoma cells. MPP(+) induced ... More
Altered oxidative product formation in neutrophils of patients recovering from therapy for acute leukemia.
AuthorsPowell BL, Olbrantz P, Bicket D, Bass DA
JournalBlood
PubMed ID3708156
'During chemotherapy for acute leukemias, severe neutropenia allows acquisition of life-threatening infections that are difficult to clear with antibiotics alone. With return of myelopoiesis, even severe infections often improve dramatically. We have sequentially examined oxidative metabolic responses of polymorphonuclear leukocytes (PMNL) from 30 patients with acute leukemias before induction chemotherapy ... More
Role of oxidative stress generated from the mitochondrial electron transport chain and mitochondrial glutathione status in loss of mitochondrial function and activation of transcription factor nuclear factor-kappa B: studies with isolated mitochondria and rat hepatocytes.
AuthorsGarcía-Ruiz C, Colell A, Morales A, Kaplowitz N, Fernández-Checa JC
JournalMol Pharmacol
PubMed ID7476912
'Mitochondria are an important source of reactive oxygen intermediates because they are the major consumers of molecular oxygen in cells. Respiration is associated with toxicity, which is related to the activation of oxygen to reactive intermediates. The purpose of the present study was to examine the role of reduced glutathione ... More
Action of DCFH and BODIPY as a probe for radical oxidation in hydrophilic and lipophilic domain.
AuthorsYoshida Y, Shimakawa S, Itoh N, Niki E
JournalFree Radic Res
PubMed ID14567446
'Fluorogenic probes such as 2'',7''-dichlorofluorescin (DCFH) have been extensively used to detect oxidative events and to measure antioxidant capacity. At the same time, however, the inherent drawbacks of these probes such as non-specificity towards oxidizing species have been pointed out. The present study was carried out to analyze the action ... More
The effects of aliphatic (n-nonane), naphtenic (1,2, 4-trimethylcyclohexane), and aromatic (1,2,4-trimethylbenzene) hydrocarbons on respiratory burst in human neutrophil granulocytes.
AuthorsMyhre O, Vestad TA, Sagstuen E, Aarnes H, Fonnum F
JournalToxicol Appl Pharmacol
PubMed ID10986013
'This study investigates the effects of aliphatic (n-heptane, n-nonane), naphtenic (methylcyclohexane, 1,2,4-trimethylcyclohexane (TMCH)), and aromatic (methylbenzene, 1,2,4-trimethylbenzene (TMB)) hydrocarbons on respiratory burst in human granulocytes. The free radical formation was measured as 2,7-dichlorofluorescein diacetate-amplified (DCF) fluorescence, by electron paramagnetic resonance (EPR) spectroscopy and by hydroxylation of 4-hydroxybenzoate. The chemotactic peptide ... More
Kupffer cell-mediated oxidative stress on colon cancer cell line visualized by digital imaging fluorescence microscopy.
AuthorsKurose I, Saito H, Suematsu M, Fukumura D, Miura S, Morizane T, Tsuchiya M
JournalCancer Lett
PubMed ID1913616
'Temporal and spatial changes of lipid peroxides in a cultured colon cancer cell line, Colo-205 cells, were investigated after culturing with Kupffer cells by using 2'',7''-dichlorofluorescein diacetate and a digital imaging processor equipped with an inverted microscope. By this method, we successfully visualized the alteration of lipid peroxides in the ... More
Redox signaling in the growth and development of colonial hydroids.
AuthorsBlackstone NW
JournalJ Exp Biol
PubMed ID12517982
'Redox signaling provides a quick and efficient mechanism for clonal or colonial organisms to adapt their growth and development to aspects of the environment, e.g. the food supply. A 'signature' of mitochondrial redox signaling, particularly as mediated by reactive oxygen species (ROS), can be elucidated by experimental manipulation of the ... More
Reactive oxygen species are crucial for hydroxychavicol toxicity toward KB epithelial cells.
AuthorsJeng JH, Wang YJ, Chang WH, Wu HL, Li CH, Uang BJ, Kang JJ, Lee JJ, Hahn LJ, Lin BR, Chang MC
JournalCell Mol Life Sci
PubMed ID14704856
'Betel quid (BQ) chewing shows a strong correlation to the incidence of oral submucous fibrosis (OSF), leukoplakia and oral cancer. BQ contains mainly areca nut, lime, Piper betle leaf (PBL) and the inflorescence of P. betle (IPB). Hydroxychavicol (4-allyl-catechol, HC), as a major phenolic compound in PBL and IPB, is ... More
Evidence for free radical formation during the oxidation of 2'-7'-dichlorofluorescin to the fluorescent dye 2'-7'-dichlorofluorescein by horseradish peroxidase: possible implications for oxidative stress measurements.
AuthorsRota C, Chignell CF, Mason RP
JournalFree Radic Biol Med
PubMed ID10515592
'The oxidation of 2''-7''-dichlorofluorescin (DCFH) to the fluorescent 2''-7''-dichlorofluorescein (DCF) by horseradish peroxidase (HRP) was investigated by fluorescence, absorption, and electron spin resonance spectroscopy (ESR). As has been previously reported, HRP/H2O2 oxidized DCFH to the highly fluorescent DCF. However, DCF fluorescence was still observed when H2O2 was omitted, although its ... More
Attenuation of oxidant stress during reoxygenation by AMP 579 in cardiomyocytes.
AuthorsXu Z, Cohen MV, Downey JM, Vanden Hoek TL, Yao Z
JournalAm J Physiol Heart Circ Physiol
PubMed ID11709426
'AMP 579, an adenosine A(1)/A(2) receptor agonist, has a strong anti-infarct effect when administered just before reperfusion. Because oxidative stress has been proposed to contribute to myocardial reperfusion injury, we tested whether AMP 579 can reduce the production of reactive oxidant species (ROS) during reoxygenation in cultured chick embryonic cardiomyocytes. ... More
Contribution of oxygen radicals to altered NO-dependent pulmonary vasodilation in acute and chronic hypoxia.
AuthorsJernigan NL, Resta TC, Walker BR
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID14672919
'Chronic hypoxia (CH) increases pulmonary arterial endothelial nitric oxide (NO) synthase (NOS) expression and augments endothelium-derived nitric oxide (EDNO)-dependent vasodilation, whereas vasodilatory responses to exogenous NO are attenuated in CH rat lungs. We hypothesized that reactive oxygen species (ROS) inhibit NO-dependent pulmonary vasodilation following CH. To test this hypothesis, we ... More
An automated micro-fluorometric assay for monitoring oxidative burst activity of phagocytes.
AuthorsWan CP, Myung E, Lau BH
JournalJ Immunol Methods
PubMed ID8445246
'A micro-fluorometric assay using 2'',7''-dichlorofluorescein diacetate (DCFH-DA) to monitor oxidative burst (OB) in phagocytes has been developed. This assay is based on the oxidation of nonfluorescent DCFH-DA to highly fluorescent 2'',7''-dichlorofluorescein (DCF) both intracellularly and extracellularly. A murine macrophage cell line, J774, and a human monocytic cell line, Mono Mac ... More
A redox signaling mechanism for density-dependent inhibition of cell growth.
'Reactive oxygen species (ROS) have recently drawn significant attention as putative mitogenic mediators downstream of activated growth factor receptors and oncogenic Ras; however, the possibility that a redox-related mechanism also operates in the negative control of cell proliferation by inhibitory signals has not been investigated thus far. Here we show ... More
Flow cytometric analysis of cell suspensions exposed to shock waves in the presence of the radical sensitive dye hydroethidine.
AuthorsEndl E, Steinbach P, Hofstädter F
JournalUltrasound Med Biol
PubMed ID7571150
'The occurrence of intracellularly and extracellularly generated free radicals during shock wave exposure on an experimental Siemens lithotripter was tested with the radical sensitive dyes hydroethidine and dichlorofluorescin (DCFH). DCFH, a nonfluorescent compound, is oxidised to dichlorfluorescein (DCF) by hydrogen peroxide in the presence of peroxidase. DCF green fluorescence intensity ... More
Increased intracellular reactive oxygen species in patients with end-stage renal failure: effect of hemodialysis.
AuthorsTepel M, Echelmeyer M, Orie NN, Zidek W
JournalKidney Int
PubMed ID10916112
'BACKGROUND: Reactive oxygen species (ROS) have been implicated in various forms of cellular injury. ROS may cause cell damage and are involved in the pathophysiology of several diseases, including atherosclerosis and chronic inflammation. METHODS: Disturbances of intracellular ROS levels were investigated in 28 patients with end-stage renal failure. The intracellular ... More
Genotoxic and antiapoptotic effect of nicotine on human gingival fibroblasts.
AuthorsArgentin G, Cicchetti R
JournalToxicol Sci
PubMed ID14718647
'Growing evidence suggests that nicotine, the addictive component of cigarettes, can have a direct role in tumor development by enhancing cell proliferation and impairing apoptotic process in certain types of human cancer cell lines. Since the correlation between apoptosis and DNA damage is already well documented, we investigated the response ... More
Expression of functional IL 2 receptors by lipopolysaccharide and interferon-gamma stimulated human monocytes.
AuthorsHolter W, Goldman CK, Casabo L, Nelson DL, Greene WC, Waldmann TA
JournalJ Immunol
PubMed ID3106493
'Human peripheral blood monocytes were stimulated with lipopolysaccharide (LPS) and interferon-gamma (IFN-gamma) alone or in combination. Stimulated but not resting monocytes displayed the Tac peptide of the interleukin 2 (IL 2) receptor within 24 hr as measured by immunofluorescence staining and [3H] Tac binding. The total number of anti-Tac binding ... More
The BCR/ABL tyrosine kinase induces production of reactive oxygen species in hematopoietic cells.
AuthorsSattler M, Verma S, Shrikhande G, Byrne CH, Pride YB, Winkler T, Greenfield EA, Salgia R, Griffin JD
JournalJ Biol Chem
PubMed ID10833515
'The BCR/ABL oncogene causes chronic myelogenous leukemia, a myeloproliferative disorder characterized by clonal expansion of hematopoietic progenitor cells and myeloid cells. It is shown here that transformation of the hematopoietic cell lines Ba/F3, 32Dcl3, and MO7e with BCR/ABL results in an increase in reactive oxygen species (ROS) compared with quiescent, ... More
Cell death caused by selenium deficiency and protective effect of antioxidants.
AuthorsSaito Y, Yoshida Y, Akazawa T, Takahashi K, Niki E
JournalJ Biol Chem
PubMed ID12888577
'Selenium is an essential trace element and it is well known that selenium is necessary for cell culture. However, the mechanism underlying the role of selenium in cellular proliferation and survival is still unknown. The present study using Jurkat cells showed that selenium deficiency in a serum-free medium decreased the ... More
C-phycocyanin protects cerebellar granule cells from low potassium/serum deprivation-induced apoptosis.
AuthorsRimbau V, Camins A, Pubill D, Sureda FX, Romay C, González R, Jiménez A, Escubedo E, Camarasa J, Pallàs M
JournalNaunyn Schmiedebergs Arch Pharmacol
PubMed ID11534860
'We tested the potential cytoprotective role of C-phycocyanin in rat cerebellar granule cell cultures. Cell death was induced by potassium and serum (K/S) withdrawal. Cell viability was studied using the neutral red assay and laser scanning cytometry with propidium iodide as fluorochrome. C-phycocyanin (1-3 mg/ml) showed a neuroprotective effect against ... More
The mechanism of cytoprotective action of lazaroids I: Inhibition of reactive oxygen species formation and lethal cell injury during periods of energy depletion.
AuthorsTaylor BM, Fleming WE, Benjamin CW, Wu Y, Mathews WR, Sun FF
JournalJ Pharmacol Exp Ther
PubMed ID8786555
'Tirilazad mesylate and related compounds, known as lazaroids, are recognized as inhibitors of membrane lipid peroxidation that also act as free radical scavengers. These compounds have demonstrated protective activity in animal models of traumatic head injury and cerebral ischemia. In this study we used an in vitro cell model to ... More
Heat-induced cellular damage and tolerance in combination with adriamycin for the PC-3 prostate cancer cell line: relationships with cytotoxicity, reactive oxygen species and heat shock protein 70 expression.
AuthorsMoriyama-Gonda N, Igawa M, Shiina H, Urakami S, Wada Y, Terashima M
JournalEur Urol
PubMed ID10895018
'OBJECTIVE: To analyze the relationships among the antitumor effect of chemothermotherapy, generation of reactive oxygen species (ROS), and expression of heat shock protein 70 (HSP-70) in the PC-3 prostate cancer cell line. MATERIALS AND METHODS: Changes in cell proliferation, cell cycle fractions, intracellular ROS accumulation and HSP-70 expression were examined ... More
Flow cytometric quantitation of oxidative product formation by polymorphonuclear leukocytes during phagocytosis.
AuthorsSzejda P, Parce JW, Seeds MS, Bass DA
JournalJ Immunol
PubMed ID6491287
'Stimulation of the oxidative metabolic burst of human polymorphonuclear leukocytes (PMNL) may occur by an all-or-none trigger mechanism or by a graded response to increasing stimulation of an individual cell. If the proposed all-or-none mechanism occurred during phagocytosis, a PMNL would expend all of its metabolic potential at once, yet ... More
Doxorubicin paradoxically protects cardiomyocytes against iron-mediated toxicity: role of reactive oxygen species and ferritin.
AuthorsCorna G, Santambrogio P, Minotti G, Cairo G
JournalJ Biol Chem
PubMed ID14739295
'The cardiotoxicity induced by the anticancer anthracycline doxorubicin (DOX) is attributed to reactions between iron and reactive oxygen species (ROS) that lead to oxidative damage. We found that DOX forms ROS in H9c2 cardiomyocytes, as shown by dichlorodihydrofluorescein oxidation and the expression of stress-responsive genes such as catalase or aldose ... More
Hazards of antioxidant combinations containing superoxide dismutase.
AuthorsPaller MS, Eaton JW
JournalFree Radic Biol Med
PubMed ID7797096
'Oxygen free radical scavengers protect against ischemia/reperfusion injury of the kidney in vivo and against hypoxia/reoxygenation (H/R) injury of renal cells in several in vitro systems. In an attempt to maximize renal protection we tested several antioxidants in combination; the individual components had previously reduced reoxygenation injury of hypoxic renal ... More
Anticonvulsant valproic acid inhibits cardiomyocyte differentiation of embryonic stem cells by increasing intracellular levels of reactive oxygen species.
AuthorsNa L, Wartenberg M, Nau H, Hescheler J, Sauer H
JournalBirth Defects Res A Clin Mol Teratol
PubMed ID12797459
'BACKGROUND: The anticonvulsant valproic acid (VPA) exerts teratogenic properties and has been demonstrated to cause neural tube defects and malformations of the heart. The effect of VPA on the differentiation of cardiomyocytes from pluripotent murine embryonic stem cells (ES cells) was investigated. METHODS: Embryoid bodies derived from ES cells were ... More
Reactive oxygen species in chick hair cells after gentamicin exposure in vitro.
AuthorsHirose K, Hockenbery DM, Rubel EW
JournalHear Res
PubMed ID9119753
'Reactive oxygen species have been invoked as a causative agent of cell death in many different developmental and pathological states. The presence of free radicals and their importance of hair cell death due to aminoglycosides is suggested by a number of studies that have demonstrated a protective effect of antioxidants. ... More
Epidermal growth factor (EGF)-induced generation of hydrogen peroxide. Role in EGF receptor-mediated tyrosine phosphorylation.
AuthorsBae YS, Kang SW, Seo MS, Baines IC, Tekle E, Chock PB, Rhee SG
JournalJ Biol Chem
PubMed ID8995250
'Recent evidence indicates that reactive oxygen species (ROS) may function as intracellular messengers in receptor signaling pathways. The possible role of ROS in epidermal growth factor (EGF) signaling was therefore investigated. Stimulation of A431 human epidermoid carcinoma cells with EGF resulted in a transient increase in the intracellular concentration of ... More
Effect of Polyoxidonium on the Phagocytic Activity of Human Peripheral Blood Leukocytes.
'The effect of polyoxidonium on the functional activity of human peripheral blood phagocytes was studied in vitro. Polyoxidonium is an N-oxidized polyethylene-piperazine derivative, a water-soluble high-molecular synthetic immunomodulator. It was established that a one-hour incubation of leukocytes with polyoxidonium increases the ability of leukocytes to kill the ingested Staphylococcus aureus ... More
The use of fluorescent probes to assess oxidative processes in isolated-perfused rat heart tissue.
AuthorsKehrer JP, Paraidathathu T
JournalFree Radic Res Commun
PubMed ID1505782
'The formation of reactive oxygen species (ROS) in intact heart tissue has been assessed by direct ESR measurements, and indirectly by the formation of characteristic tissue products and the protective effects of various antioxidants. The development of lipid soluble esters of compounds which can be trapped intracellularly after hydrolysis, and ... More
Time interval gating for analysis of cell function using flow cytometry.
'We propose a method which significantly shortens the time required for both the collection and analysis of data derived from multiple sample, flow cytometric kinetic assays. We have defined the term Time Interval Gating (TIG) to describe this method. TIG effectively allows one flow cytometer to concurrently monitor several samples ... More
Spectrophotometric assay for total peroxyl radical-trapping antioxidant potential in human serum.
AuthorsValkonen M, Kuusi T
JournalJ Lipid Res
PubMed ID9144097
'Antioxidants prevent modification of low density lipoprotein (LDL) by free radicals and possibly also atheroma formation. The capacity of human serum to resist attacks by free radicals is measured by the total peroxyl radical-trapping potential (TRAP). Its measurement has thus far required equipment not available in many clinical laboratories such ... More
Transferrin receptor-dependent iron uptake is responsible for doxorubicin-mediated apoptosis in endothelial cells: role of oxidant-induced iron signaling in apoptosis.
AuthorsKotamraju S, Chitambar CR, Kalivendi SV, Joseph J, Kalyanaraman B
JournalJ Biol Chem
PubMed ID11856741
'In the past, investigators have successfully used iron chelators to mitigate the cardiotoxicity of doxorubicin (DOX), a widely used anticancer drug that induces reactive oxygen species (ROS), oxidative damage, and apoptosis. Although intracellular iron plays a critical role in initiating DOX-induced apoptosis, the molecular mechanism(s) that link iron, ROS, and ... More
Flow cytometric measurement of mitochondrial mass and function: a novel method for assessing chemoresistance.
AuthorsMancini M, Sedghinasab M, Knowlton K, Tam A, Hockenbery D, Anderson BO
JournalAnn Surg Oncol
PubMed ID9607633
'BACKGROUND: Chemotherapeutic agents induce apoptosis in cancer cells. Drugs failing to induce apoptosis are likely to have decreased clinical efficacy. We hypothesize that (1) chemotherapeutic agents induce mitochondrial changes and apoptosis through mechanisms associated with reactive oxidant species production; (2) the anti-apoptotic protein Bcl-2 prevents drug-induced mitochondrial changes, reactive oxygen ... More
Flow cytometric detection of hydrogen peroxide production induced by doxorubicin in cancer cells.
AuthorsUbezio P, Civoli F
JournalFree Radic Biol Med
PubMed ID8005536
'2'',7''-Dichlorofluorescin diacetate (DCFH-DA) has been previously used to study the oxidative burst of neutrophils induced by different stimuli. The method is based on the fact that DCFH-DA diffuses through the cell membrane and it is hydrolyzed by intracellular esterases to DCFH, which remains trapped within the cells. DCFH, a nonfluorescent ... More
Oxidation pathways for the intracellular probe 2',7'-dichlorofluorescein.
'The oxidation of 2'',7''-dichlorofluorescin (DCFH) to a fluorescent product is currently used to evaluate oxidant stress in cells. However, there is considerable uncertainty as to the enzymatic and nonenzymatic pathways that may result in DCFH oxidation. Iron/hydrogen peroxide-induced DCFH oxidation was inhibited by catalase or by the hydroxyl radical scavenger ... More
Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean?
AuthorsHalliwell B, Whiteman M
JournalBr J Pharmacol
PubMed ID15155533
'Free radicals and other reactive species (RS) are thought to play an important role in many human diseases. Establishing their precise role requires the ability to measure them and the oxidative damage that they cause. This article first reviews what is meant by the terms free radical, RS, antioxidant, oxidative ... More
Glutamate induces the production of reactive oxygen species in cultured forebrain neurons following NMDA receptor activation.
AuthorsReynolds IJ, Hastings TG
JournalJ Neurosci
PubMed ID7751912
'Using the fluorescent dye 2'',7''-dichlorodihydrofluorescein (DCF-H2) we investigated the role of glutamate in the production of reactive oxygen species (ROS) in cultured neurons from fetal rat forebrain. The addition of an excitotoxic concentration of glutamate (100 microM) produced a generalized decrease in cellular DCF fluorescence accompanied by local areas of ... More
Association of gastric epithelial apoptosis with the ability of Helicobacter pylori to induce a neutrophil oxidative burst.
AuthorsMizuki I, Shimoyama T, Fukuda S, Liu Q, Nakaji S, Munakata A
JournalJ Med Microbiol
PubMed ID10847205
'Both polymorphonuclear cell infiltration and increased epithelial apoptosis are seen in gastric mucosa in the presence of Helicobacter pylori infection. This study examined the association between bacterial ability to stimulate an oxidative burst in neutrophils and epithelial apoptosis. Biopsy specimens were obtained from 15 patients to detect apoptotic cells by ... More
Intracellular photobleaching of 5,10,15,20-tetrakis(m-hydroxyphenyl) chlorin (Foscan) exhibits a complex dependence on oxygen level and fluence rate.
AuthorsKunz L, MacRobert AJ
JournalPhotochem Photobiol
PubMed ID11837325
'The understanding of photosensitizer photobleaching is important not only for mechanistic studies, but also for the development of monitoring techniques for clinical dosimetry in photodynamic therapy. In this study, we investigated the intracellular photobleaching of 5,10,15,20-tetrakis(m-hydroxyphenyl)chlorin (mTHPC, Foscan) in the murine macrophage cell line J774A.1, using quantitative fluorescence imaging microscopy, ... More
Adenosine A1 receptor activation reduces reactive oxygen species and attenuates stunning in ventricular myocytes.
AuthorsNarayan P, Mentzer RM, Lasley RD
JournalJ Mol Cell Cardiol
PubMed ID11133228
'Reactive oxygen species (ROS) formation following brief periods of ischemia or hypoxia is thought to be the underlying cause of myocardial stunning. Adenosine A1 receptor activation prior to ischemia/hypoxia attenuates stunning, although the mechanism for this effect remains unknown. Isolated rat ventricular myocytes loaded with the ROS-sensitive indicator dichlorofluorescin were ... More
Cocaine-mediated enhancement of Tat toxicity in rat hippocampal cell cultures: the role of oxidative stress and D1 dopamine receptor.
AuthorsAksenov MY, Aksenova MV, Nath A, Ray PD, Mactutus CF, Booze RM
JournalNeurotoxicology
PubMed ID16386305
'It is becoming widely accepted that psychoactive drugs can significantly alter the progression of neuropathological changes in the HIV-infected brain. The use of cocaine can aggravate the neurotoxic effects of HIV-1 proteins such as HIV-1 transactivating protein Tat and virus'' envelope protein gp120. HIV-1 Tat is believed to play an ... More
The use of N-t-butyl hydroxylamine for radioprotection in cultured cells and mice.
AuthorsLee JH, Kim IS, Park JW,
JournalCarcinogenesis
PubMed ID15016661
'Exposure of cells to ionizing radiation leads to formation of reactive oxygen species (ROS) that are associated with radiation-induced cytotoxicity. Therefore, compounds that scavenge ROS may confer radioprotective effects. Recently, it has been shown that the decomposition product of the spin-trapping agent alpha-phenyl-N-t-butylnitrone (PBN), N-t-butyl hydroxylamine (NtBHA), mimics PBN and ... More
Detection of reactive oxygen species (ROS) and apoptosis in human fragmented embryos.
'In human in-vitro fertilization (IVF)-embryo transfer, the in-vitro culture environment differs from in-vivo conditions in that the oxygen concentration is higher, and in such conditions the mouse embryos show a higher concentration of reactive oxygen species (ROS) in simple culture media. ROS are believed to cause damage to cell membranes ... More
Aromatic monoamine-induced immediate oxidative burst leading to an increase in cytosolic Ca2+ concentration in tobacco suspension culture.
AuthorsKawano T, Pinontoan R, Uozumi N, Miyake C, Asada K, Kolattukudy PE, Muto S
JournalPlant Cell Physiol
PubMed ID11092910
'Aromatic monoamines may contribute to both chemical and physical protection of plants. Addition of phenylethylamine (PEA) and benzylamine to tobacco suspension culture (cell line BY-2) induced a very rapid and transient generation of two active oxygen species (AOS), H2O2 and superoxide anion, both detected with chemiluminescence. Electron spin resonance spectroscopy ... More
Characterization of cyclopropane fatty-acid synthase from Sterculia foetida.
'Cyclopropane synthase from Sterculia foetida developing seeds catalyzes the addition of a methylene group from S-adenosylmethionine to the cis double bond of oleic acid (Bao, X., Katz, S., Pollard, M., and Ohlrogge, J. (2002) Proc. Natl. Acad. Sci. U. S. A. 99, 7172-7177). To understand this enzyme better, differential expression ... More
Redox regulation of glutathione S-transferase induction by benzyl isothiocyanate: correlation of enzyme induction with the formation of reactive oxygen intermediates.
AuthorsNakamura Y, Ohigashi H, Masuda S, Murakami A, Morimitsu Y, Kawamoto Y, Osawa T, Imagawa M, Uchida K
JournalCancer Res
PubMed ID10667562
'Here we report the molecular mechanism underlying the induction of glutathione S-transferase (GST) in rat liver epithelial RL34 cells treated with a cancer chemopreventive isothiocyanate compound, benzylisothiocyanate (BITC). BITC was found to significantly induce GST activity in RL34 cells. Northern and Western blot analyses demonstrated that BITC specifically enhanced the ... More
Regulation of human neutrophil type 3 complement receptor (iC3b receptor) expression during phagocytosis of Staphylococcus aureus and Escherichia coli.
AuthorsGordon DL, Rice JL, McDonald PJ
JournalImmunology
PubMed ID2527804
'Human neutrophils (PMN) express a receptor for iC3b, a cleavage product of C3b. CR3 is an important receptor for phagocytosis of opsonized bacteria and its expression is enhanced by cell activation. We examined PMN CR3 expression during phagocytosis using flow cytometry and a CR3-specific monoclonal antibody. After 30 min phagocytosis ... More
Relationship between the intracellular reactive oxygen species and the induction of oxidative DNA damage in human neutrophil-like cells.
AuthorsTakeuchi T, Nakajima M, Morimoto K
JournalCarcinogenesis
PubMed ID8761407
'To clarify the mechanisms of intracellular induction of oxidative DNA damage, we have investigated the concentrations of intracellular reactive oxygen species and the amounts of 8-hydroxydeoxyguanosine (8OHdG), a mutagenic oxidative DNA damage, in human neutrophil-like cells, dimethylsulfoxide-differentiated HL60 (DMSO-HL60). We determined intracellular concentrations of hydrogen peroxide and superoxide by flow ... More
N-t-butyl hydroxylamine, a hydrolysis product of alpha-phenyl-N-t-butyl nitrone, is more potent in delaying senescence in human lung fibroblasts.
AuthorsAtamna H, Paler-Martínez A, Ames BN
JournalJ Biol Chem
PubMed ID10702229
'Alpha-phenyl-N-t-butyl nitrone (PBN), a spin trap, scavenges hydroxyl radicals, protects tissues from oxidative injury, and delays senescence of both normal human lung fibroblasts (IMR90) and senescence-accelerated mice. N-t-butyl hydroxylamine and benzaldehyde are the breakdown products of PBN. N-t-Butyl hydroxylamine delays senescence of IMR90 cells at concentrations as low as 10 ... More
Preconditioning in cardiomyocytes protects by attenuating oxidant stress at reperfusion.
'Cardiomyocyte death after ischemia/reperfusion correlates with oxidant stress, and antioxidants confer protection in that model. Preconditioning (PC) with hypoxia or adenosine also confers protection, leading us to hypothesize that PC protects by attenuating oxidant generation during subsequent ischemia/reperfusion. Chick cardiomyocytes were preconditioned with 10 minutes of hypoxia or adenosine (100 ... More
Arsenite induces apoptosis in Chinese hamster ovary cells by generation of reactive oxygen species.
AuthorsWang TS, Kuo CF, Jan KY, Huang H
JournalJ Cell Physiol
PubMed ID8908193
'Arsenic, a human carcinogen, possesses a serious environmental threat but the mechanism of its toxicity remains unclear. Knowledge of how arsenic induces cell death and how cells escape the death path may help to understand arsenic carcinogenesis. We have investigated the nature of sodium arsenite-induced cell death in Chinese hamster ... More
Copper-induced formation of reactive oxygen species causes cell death and disruption of calcium homeostasis in trout hepatocytes.
AuthorsManzl C, Enrich J, Ebner H, Dallinger R, Krumschnabel G
JournalToxicology
PubMed ID15036756
'We have previously shown that copper is acutely toxic for trout hepatocytes, inducing enhanced influx of Ca(2+) and a loss of cell viability. The aim of the present study was to elucidate the pathways of Ca(2+) entry into the cells, the hypothetical role of reactive oxygen species (ROS) in copper ... More