Regulation of latent sensory hair cell precursors by glia in the zebrafish lateral line.
AuthorsGrant KA, Raible DW, Piotrowski T,
JournalNeuron
PubMed ID15629703
'The lateral line is a placodally derived mechanosensory organ in anamniotes that detects the movement of water. In zebrafish embryos, a migrating primordium deposits seven to nine clusters of sensory hair cells, or neuromasts, at intervals along the trunk. Postembryonically, neuromasts continue to be added. We show that some secondary ... More
Mechanism of uptake of the fluorescent dye 2-(4-dimethylaminostyryl)-1-ethylpyridinium cation (DMP+) by phospholipid vesicles.
AuthorsSedgwick EG, Bragg PD
JournalBiochim Biophys Acta
PubMed ID8443217
'The fluorescent dye 2-(4-dimethylaminostyryl)-1-ethylpyridinium cation (DMP+) is taken up by liposomes of egg phosphatidylcholine in response to the imposition of a transmembrane potential. Entry of DMP+ into the bilayer driven by the transmembrane potential is accompanied by a change in the fluorescence emission maximum of the dye. This change reflects ... More
Regenerated hair cells can originate from supporting cell progeny: evidence from phototoxicity and laser ablation experiments in the lateral line system.
AuthorsBalak KJ, Corwin JT, Jones JE
JournalJ Neurosci
PubMed ID2388077
'The mechanisms that lead to the production of sensory hair cells during regeneration have been investigated by using 2 different procedures to ablate preexisting hair cells in individual neuromast sensory epithelia of the lateral line in the tails of salamanders, then monitoring the responses of surviving cells. In one series ... More
Fluorescent labelling of Na+, K(+)-ATPase in intact cells by use of a fluorescent derivative of ouabain: salinity and teleost chloride cells.
AuthorsMcCormick SD
JournalCell Tissue Res
PubMed ID2164884
'Anthroylouabain, a fluorescent derivative of ouabain, was used to localize Na+, K(+)-ATPase in transport epithelia of two species of teleosts. Exposure of the opercular membrane of seawater-adapted tilapia (Oreochromis mossambicus) and the jaw skin of the long-jawed mudsucker (Gillichthys mirabilis) to a 2 microM anthroylouabain solution resulted in the appearance ... More
Selective retention of the fluorescent dye DASPEI in a larval gastropod mollusc after paraformaldehyde fixation.
AuthorsLeise EM
JournalMicrosc Res Tech
PubMed ID8800755
'In the vertebrates, the vital mitochondrial dye DASPEI (2-(4-dimethylaminostyryl)-N-ethylpyridinium iodide) has been used for the rapid visualization of several distinct classes of epidermal cells in vivo and in vitro: epidermal electroreceptors, mechanoreceptors, and chloride cells in teleosts, and mechanoreceptors in amphibians. I used DASPEI in an attempt to locate a ... More
Mitochondrial uncoupling protein may participate in futile cycling of pyruvate and other monocarboxylates.
AuthorsJezek P, Borecký J
JournalAm J Physiol
PubMed ID9688604
'The physiological role of monocarboxylate transport in brown adipose tissue mitochondria has been reevaluated. We studied pyruvate, alpha-ketoisovalerate, alpha-ketoisocaproate, and phenylpyruvate uniport via the uncoupling protein (UCP1) as a GDP-sensitive swelling in K+ salts induced by valinomycin or by monensin and carbonyl cyanide-p-(trifluoromethoxy)phenylhydrazone in Na+ salts. We have demonstrated that ... More
In vivo sequential changes in chloride cell morphology in the yolk-sac membrane of mozambique tilapia (Oreochromis mossambicus) embryos and larvae during seawater adaptation
AuthorsHiroi J, Kaneko T, Tanaka M
JournalJ Exp Biol
PubMed ID10574727
'Changes in chloride cell morphology were examined in the yolk-sac membrane of Mozambique tilapia (Oreochromis mossambicus) embryos and larvae transferred from fresh water to sea water. By labelling chloride cells with DASPEI, a fluorescent probe specific for mitochondria, we observed in vivo sequential changes in individual chloride cells by confocal ... More
The apical sensory organ of a gastropod veliger is a receptor for settlement cues.
AuthorsHadfield MG, Meleshkevitch EA, Boudko DY
JournalBiol Bull
PubMed ID10707814
'On the basis of anatomy and larval behavior, the apical sensory organ (ASO) of gastropod veliger larvae has been implicated as the site of perception of cues for settlement and metamorphosis. Until now, there have been no experimental data to support this hypothesis. In this study, cells in the ASO ... More
The concentration dependence of the depolarization of yeast by monovalent cations.
AuthorsBorst-Pauwels GW, van de Mortel JB, Theuvenet AP
JournalBiochim Biophys Acta
PubMed ID1504065
'Monovalent cations decrease the initial rate of uptake of the membrane potential probe 2-(dimethylaminostyryl)-1-ethyl-pyridinium (DMP) into metabolizing cells, showing that the cells are depolarized. A steep decrease in this rate was found even at low cation concentrations, reaching 62%, 42%, 58%, 40% and 40% at high concentrations of K+, Rb+, ... More
Interaction of a phosphatidylcholine derivative of 1,6-diphenyl-1,3,5-hexatriene (DPH-PC) with lymphocytes.
AuthorsFerretti G, Tangorra A, Curatola G
JournalBiochem Int
PubMed ID1558541
'In this study we have demonstrated a transfer of a phosphatidylcholine derivative of 1,6-diphenyl-1,3,5-hexatriene (DPH-PC) from self-quenched lipid vesicles to intact lymphocytes. Membrane labeling was followed measuring the time dependent reexpression of fluorescence. The results of fluorescence quenching by 2,4,6-trinitrobenzene sulfonate and the decrease of fluorescence polarization values during incubation ... More
2-(Dimethylaminostyryl)-1-ethylpyridinium iodide: a fluorescent probe of energetic conditions in brown-adipose-tissue mitochondria.
AuthorsRafael J
JournalHoppe Seylers Z Physiol Chem
PubMed ID7380388
'2-(Dimethylaminostyryl)-1-ethylpyridinium iodide is shown to be a fluorescent monitor for the energetic state of isolated brown-adipose-tissue mitochondria. A reproducible fluorescence parameter of mitochondrial coupling conditions is established by relating the fluorescence intensity as measured under specific experimental conditions to the maximum fluorescence response displayed by the mitochondria under state 4-conditions ... More
Applications of dye-sensitized photoreactions in neurobiology.
AuthorsSpikes JD
JournalPhotochem Photobiol
PubMed ID1775530
Fluorescent probes that stain living nerve terminals.
AuthorsMagrassi L, Purves D, Lichtman JW
JournalJ Neurosci
PubMed ID3572476
We have evaluated the efficacy of 18 cationic mitochondrial dyes that, as a class, show some ability to stain living nerve terminals. Several of these agents provide excellent staining of neuromuscular junctions in a wide range of species. More detailed studies of the most effective of these dyes--4-(4-diethylaminostyryl)-N-methylpyridinium iodide (4-Di-2-ASP)--indicate ... More
Mechanism of energization of uptake of the fluorescent dye 2-(4-dimethylaminostyryl)-1-ethylpyridinium cation [DMP+] into an acrA strain of Escherichia coli.
AuthorsSedgwick EG, Bragg PD
JournalBiochim Biophys Acta
PubMed ID1739728
The mechanism of uptake of the fluorescent dye 2-(4-dimethylaminostyryl)-1-ethylpyridinium cation (DMP+) into cells and vesicles of the acrA strain AS-1 of Escherichia coli was examined. Uptake was energized by substrate oxidation and discharged by uncouplers. Uptake was enhanced by the presence of tetraphenylphosphonium cation, tetraphenylboron anion and tributyltin chloride, which ... More
Effective sensory modality activating an escape triggering neuron switches during early development in zebrafish.
AuthorsKohashi T, Nakata N, Oda Y,
JournalJ Neurosci
PubMed ID22539843
Developing nervous systems grow to integrate sensory signals from different modalities and to respond through various behaviors. Here, we examined the development of escape behavior in zebrafish [45-170 h postfertilization (hpf)] to study how developing sensory inputs are integrated into sensorimotor circuits. Mature fish exhibit fast escape upon both auditory/vestibular ... More
Vital imaging of glomeruli in the mouse olfactory bulb.
AuthorsLaMantia AS, Pomeroy SL, Purves D
JournalJ Neurosci
PubMed ID1545246
We have monitored the pattern of identified glomeruli in the olfactory bulbs of newborn, juvenile, and adult mice over intervals of several hours to several weeks. Our purpose was to assess the development and stability of these complex units in the mammalian brain. Glomeruli can be observed by vital fluorescent ... More
Rapid morphological oscillation of mitochondrion-rich cell in estuarine mudskipper following salinity changes.
AuthorsSakamoto T, Yokota S, Ando M
JournalJ Exp Zool
PubMed ID10766976
Morphological changes in the chloride cells or mitochondrion-rich (MR) cells in the skin under the pectoral fin of the estuarine mudskipper (Periophthalmus modestus) were examined in relation to intertidal salinity oscillation in river mouth. MR cells were distinguished between those in contact with the water (cells labeled with both mitochondrial ... More
Transient hyperpolarization of yeast by glucose and ethanol.
At pH 7, addition of glucose under anaerobic conditions to a suspension of the yeast Saccharomyces cerevisiae causes both a transient hyperpolarization and a transient net efflux of K+ from the cells. Hyperpolarization shows a peak at about 3 min and a net K+ efflux at 4-5 min. An additional ... More
A putative K(+)-selective channel in the plasma membrane of yeast that is blocked by micromolar concentrations of external divalent cations and is insensitive to tetraethylammonium.
Authorsvan de Mortel JB, Theuvenet AP, Borst-Pauwels GW
JournalBiochim Biophys Acta
PubMed ID2198940
At pH 7, addition of glucose to an anaerobic suspension of non-metabolizing yeast cells causes a transient net efflux of K+ from the cells and a concomitant transient hyperpolarization of the plasma membrane (Van de Mortel, J.B.J., et al. (1988) Biochem. Biophys. Acta 936, 421-428). Both phenomena are effectively suppressed ... More
Interplay between the efflux pump and the outer membrane permeability barrier in fluorescent dye accumulation in Pseudomonas aeruginosa.
AuthorsGerm M, Yoshihara E, Yoneyama H, Nakae T
JournalBiochem Biophys Res Commun
PubMed ID10425205
Pseudomonas aeruginosa encodes three types of xenobiotic efflux pumps, MexAB-OprM, MexCD-OprJ, and MexEF-OprN, which are regulated by the nalB, nfxB, and nfxC genes, respectively, and their high expression renders the cells resistant to multiple species of antibiotics. We evaluated the role of the outer membrane permeability barrier and the efflux ... More
The use and effects of vital fluorescent dyes: observation of motor nerve terminals and satellite cells in living frog muscles.
AuthorsHerrera AA, Banner LR
JournalJ Neurocytol
PubMed ID2351996
Several different fluorescent mitochondrial dyes were tested as vital stains for motor nerve terminals and other cells in frog skeletal muscles. It was found that 3,3' diethyloxadicarbocyanine iodide and 4-(4-diethylaminostyryl)-N-methylpyridinium iodide were most useful. Both dyes labelled motor nerve terminals with high reliability. Electrophysiological and morphological control experiments showed that ... More
Alkaline pH, membrane potential, and magnesium cations are negative modulators of purine nucleotide inhibition of H+ and Cl- transport through the uncoupling protein of brown adipose tissue mitochondria.
AuthorsJezek P, Houstek J, Drahota Z
JournalJ Bioenerg Biomembr
PubMed ID2463983
Modulators of purine nucleotide (PN) inhibition of H+ and Cl- transport mediated by the uncoupling protein (UP) of brown adipose tissue (BAT) mitochondria were studied: Alkalinization strongly diminishes GDP inhibition of H+ transport (delta log IC50 = -delta pHout), while more intensive inhibition of Cl- transport is only slightly altered. ... More
Endothelin 1-mediated regulation of pharyngeal bone development in zebrafish.
AuthorsKimmel CB, Ullmann B, Walker M, Miller CT, Crump JG
JournalDevelopment
PubMed ID12588850
Endothelin 1 (Edn1), a secreted peptide expressed ventrally in the primordia of the zebrafish pharyngeal arches, is required for correct patterning of pharyngeal cartilage development. We have studied mutants and morpholino-injected larvae to examine the role of the Edn1 signal in patterning anterior pharyngeal arch bone development during the first ... More
Regeneration of sensory cells after laser ablation in the lateral line system: hair cell lineage and macrophage behavior revealed by time-lapse video microscopy.
AuthorsJones JE, Corwin JT
JournalJ Neurosci
PubMed ID8551349
The regeneration of sensory hair cells in lateral line neuromasts of axolotls was investigated via nearly continuous time-lapse microscopic observation after all preexisting hair cells were killed by a laser microbeam. The laser treatments left neuromasts with one resident cell type, which was supporting cells. Over the course of 1 ... More
Cellular and subcellular structure of anterior sensory pathways in Phestilla sibogae (Gastropoda, Nudibranchia).
AuthorsBoudko DY, Switzer-Dunlap M, Hadfield MG
JournalJ Comp Neurol
PubMed ID10075442
Two sensory-cell types, subepithelial sensory cells (SSCs) and intraepithelial sensory cells (ISCs), were identified in the anterior sensory organs (ASO: pairs of rhinophores and oral tentacles, and the anterior field formed by the oral plate and cephalic shield) of the nudibranch Phestilla sibogae after filling through anterior nerves with the ... More
Mutations affecting development of the zebrafish inner ear and lateral line.
AuthorsWhitfield TT, Granato M, van Eeden FJ, Schach U, Brand M, Furutani-Seiki M, Haffter P, Hammerschmidt M, Heisenberg CP, Jiang YJ, Kane DA, Kelsh RN, Mullins MC, Odenthal J, Nüsslein-Volhard C
JournalDevelopment
PubMed ID9007244
Mutations giving rise to anatomical defects in the inner ear have been isolated in a large scale screen for mutations causing visible abnormalities in the zebrafish embryo (Haffter, P., Granato, M., Brand, M. et al. (1996) Development 123, 1-36). 58 mutants have been classified as having a primary ear phenotype; ... More
Differential permeability for lipophilic compounds in uncoupler-resistant cells of Escherichia coli.
AuthorsSedgwick EG, Bragg PD
JournalBiochim Biophys Acta
PubMed ID1739727
The acrA strain AS-1 of Escherichia coli is more sensitive than its parent W3110 to growth inhibition by Methylene blue, sodium dodecyl sulfate and novobiocin. UR-3 is an uncoupler-resistant strain isolated from AS-1 which is resistant to growth inhibition by carbonylcyanide m-chlorophenylhydrazone (CCCP), 3,3',4',5-tetrachlorsalicylanilide (TCS) and tributyltin chloride, while remaining ... More
The role of efflux systems and the cell envelope in fluorescence changes of the lipophilic cation 2-(4-dimethylaminostyryl)-1-ethylpyridinium in Escherichia coli.
AuthorsSedgwick EG, Bragg PD
JournalBiochim Biophys Acta
PubMed ID8593278
The interaction of the fluorescent dye 2-(4-dimethylaminostyryl)-1-ethlypyridinium cation (DMP+) with cells of Escherichia coli AN120 (uncA) and AS-1 (acrA) was studied to elucidate the role of the envelope and of efflux systems in the uptake of lipophilic cations. DMP+ bound to the two strains in a different manner. With AS-1 ... More
Mitochondrial function is involved in LDL oxidation mediated by human cultured endothelial cells.
AuthorsMabile L, Meilhac O, Escargueil-Blanc I, Troly M, Pieraggi MT, Salvayre R, Nègre-Salvayre A
JournalArterioscler Thromb Vasc Biol
PubMed ID9301638
Human endothelial cells (ECs) grown under standard conditions are able to generate a basal level of oxygen free radicals and induce progressive oxidation of LDLs. Inhibition of cell-mediated LDL oxidation by superoxide dismutase, EDTA, or desferrioxamine implicates a role for superoxide anion and/or transition metals in this process. The potential ... More
Hemodynamics and mitochondrial energy metabolism in right heart hypertrophy after acute hypoxic stress.
AuthorsThürich T, Bereiter-Hahn J, Schneider M, Zimmer G
JournalArzneimittelforschung
PubMed ID10219464
Excessive right heart hypertrophy was investigated under additional acute hypoxic stress to find out a possible contribution of mitochondrial dysfunction to sudden heart failure. Severe right heart hypertrophy in rats was induced by exposure to hypobaric pressure (46,663 Pa) for 4 weeks. Heart rate, isovolumic pressure and coronary flow were ... More
Use of fluorescence probes to monitor function of the subunit proteins of the MexA-MexB-oprM drug extrusion machinery in Pseudomonas aeruginosa.
AuthorsOcaktan A, Yoneyama H, Nakae T
JournalJ Biol Chem
PubMed ID9268332
The MexA-MexB-OprM efflux pump of Pseudomonas aeruginosa consists of two inner membrane proteins, MexA and MexB, and one outer membrane protein, OprM. We investigated the role of the components of this drug extrusion system by evaluating the repercussions of deleting these subunit components on the accumulation of several fluorescent probes. ... More