Interaction between Ca2+ and dipalmitoylphosphatidylcholine membranes. II. Fluorescence anisotropy study.
AuthorsKataoka R, Aruga S, Mitaku S, Kinosita K, Ikegami A
JournalBiophys Chem
PubMed ID3838686
The effect of Ca2+ on the molecular mobility in dipalmitoylphosphatidylcholine membranes was studied by steady-state and time-resolved measurements of fluorescence anisotropy. The fluorescence anisotropy decay of 1,6-diphenyl-1,3,5-hexatriene in the hydrocarbon region indicated that the free volume of molecular rotation became more restricted when the Ca2+ concentration was increased. The decrease ... More
Lipid dynamics and lipid-protein interactions in rat enterocyte basolateral and microvillus membranes.
AuthorsBrasitus TA, Schachter D
JournalBiochemistry
PubMed ID6249340
Importance of phosphatidylethanolamine for association of protein kinase C and other cytoplasmic proteins with membranes.
AuthorsBazzi MD, Youakim MA, Nelsestuen GL
JournalBiochemistry
PubMed ID1734960
'Biological membranes exhibit an asymmetric distribution of phospholipids. Phosphatidylserine (PS) is an acidic phospholipid that is found almost entirely on the interior of the cell where it is important for interaction with many cellular components. A less well understood phenomenon is the asymmetry of the neutral phospholipids, where phosphatidylcholine (PC) ... More
Mechanism of activation of protein kinase C: roles of diolein and phosphatidylserine.
AuthorsMosior M, Epand RM
JournalBiochemistry
PubMed ID8418861
'We studied the roles of lipid concentration, phosphatidylserine (PS), and diolein (DG) contents, as well as Ca2+ concentration, on the partitioning of protein kinase C (PKC) between aqueous and membrane environments as well as the relationship of this partitioning to the activation of the enzyme. Physiological concentrations of 1 mol ... More
Effect of n-alkanols on lipid bilayer hydration.
AuthorsHo C, Stubbs CD
JournalBiochemistry
PubMed ID9271493
'The effect of a homologous series of aliphatic n-alkanols on the presence of water within the head group and acyl chain region of lipid bilayers was investigated using time-resolved fluorescence spectroscopy according to a previously published approach [Ho, C., Slater, S. J., & Stubbs, C. D. (1995) Biochemistry 34, 6188-6195]. ... More
The adipocyte fatty acid-binding protein binds to membranes by electrostatic interactions.
AuthorsSmith ER, Storch J
JournalJ Biol Chem
PubMed ID10585398
'The adipocyte fatty acid-binding protein (AFABP) is believed to transfer unesterified fatty acids (FA) to phospholipid membranes via a collisional mechanism that involves ionic interactions between lysine residues on the protein surface and phospholipid headgroups. This hypothesis is derived largely from kinetic analysis of FA transfer from AFABP to membranes. ... More
Monitoring of phospholipid vesicle fusion by fluorescence energy transfer between membrane-bound dye labels.
AuthorsVanderwerf P, Ullman EF
JournalBiochim Biophys Acta
PubMed ID6766742
'A sensitive method which utilizes fluorescence energy transfer to assay Ca2+ -or Mg2+ -mediated fusion of phospholipid vesicles is reported. More than 85% quenching results when phosphatidylserine vesicles labelled with dansyl phosphatidylethanolamine (donor) are fused with vesicles labelled with rhodamine phosphatidylethanolamine (acceptor) in the presence of 5 mM CaCl2 or ... More
Fluorescence studies on the lipoprotein complex of the fatty acid synthetase from the insect Ceratitis capitata.
AuthorsGavilanes JG, Lizarbe MA, Municio AM, Oñaderra M
JournalBiochemistry
PubMed ID6895316
'The fatty acid synthetase complex from the insect Ceratitis capitata forms a stable lipoprotein complex. The intrinsic fluorescence of the complex was studied by observing the emission spectra with different excitation wavelengths, both in the native complex and after temperature with sodium cholate and sodium dodecyl sulfate. The excitation spectrum ... More
Inhibition of lipoprotein lipase activity by sphingomyelin: role of membrane surface structure.
AuthorsSaito H, Arimoto I, Tanaka M, Sasaki T, Tanimoto T, Okada S, Handa T
JournalBiochim Biophys Acta
PubMed ID10903482
'We have recently shown that sphingomyelin (SM) strongly inhibits lipoprotein lipase (LPL)-mediated lipolysis in monolayers and emulsion particles. To further evaluate how SM modulates LPL activity on the emulsion surface, the relationship between membrane surface structure and LPL activity was investigated. We measured fluorescence anisotropy of 1-palmitoyl-2-[3-(diphenylhexatrienyl)propionyl]-sn-3-phosphati dylcho line, probing ... More
Groups with polar characteristics can locate at both shallow and deep locations in membranes: the behavior of dansyl and related probes.
AuthorsAsuncion-Punzalan E, Kachel K, London E
JournalBiochemistry
PubMed ID9521780
'To understand the relationship between the chemical structure of polar molecules and their membrane location, the behavior of dansyl (dimethylaminonaphthalenesulfonyl) and related polar fluorescent probes was examined. The depth of these probes in lipid bilayers was determined by parallax analysis of fluorescence quenching [Chattopadhyay and London (1987) Biochemistry 26, 39-45; ... More
Functional interaction between Cerebratulus lacteus cytolysin A-III and phospholipase A2. Implications for the mechanism of cytolysis.
AuthorsLiu JW, Blumenthal KM
JournalJ Biol Chem
PubMed ID3360796
'A study on the interaction between bee venom phospholipase A2 and Cerebratulus lacteus cytolysin A-III, a major hemolysin secreted by this organism has been carried out. The hemolytic activity of A-III in phosphate-buffered saline is increased 5-fold in the presence of phospholipase A2 from bee venom. Dansylphosphatidylethanolamine (DPE) labeled, phosphatidylcholine-containing ... More
Solvent relaxation in lipid bilayers with dansyl probes.
AuthorsStubbs CD, Meech SR, Lee AG, Phillips D
JournalBiochim Biophys Acta
PubMed ID3995032
'The solvent relaxation properties of the dansyl group attached to two lipids (dansylphosphatidylethanolamine and dansylphosphatidylserine), a fatty acid (dansylundecanoic acid), and two drugs (dansylbenzocaine and dansylpropranolol) were compared in a variety of different lipid systems. Several methods for characterising solvent relaxation were compared in detail for dansylpropranolol in bilayer vesicles ... More
Protein kinase C interaction with calcium: a phospholipid-dependent process.
AuthorsBazzi MD, Nelsestuen GL
JournalBiochemistry
PubMed ID2271522
'The calcium-binding properties of calcium- and phospholipid-dependent protein kinase C (PKC) were investigated by equilibrium dialysis in the presence and the absence of phospholipids. Calcium binding to PKC displayed striking and unexpected behavior; the free proteins bound virtually no calcium at intracellular calcium concentrations and bound limited calcium (about 1 ... More
Modulation of phospholipase A2: identification of an inactive membrane-bound state.
AuthorsBurack WR, Gadd ME, Biltonen RL
JournalBiochemistry
PubMed ID7578091
'Phospholipase A2-catalyzed hydrolysis of vesicular phospholipid has been used to model the modulation of an enzyme''s function by membrane properties. Phospholipase A2''s (PLA2) kinetics toward large unilamellar vesicles (LUV) composed of dipalmitoylphosphatidylcholine (DPPC) are anomalous; these is a slow initial phase of catalysis (a lag) which ends abruptly with a ... More
Interactions of soluble penicillin-binding protein 2a of methicillin-resistant Staphylococcus aureus with moenomycin.
AuthorsGraves-Woodward K, Pratt RF
JournalBiochemistry
PubMed ID10441150
'Kinetics studies in homogeneous aqueous solution showed that solubilized penicillin-binding protein 2a (sPBP2a) of methicillin-resistant Staphylococcus aureus (a bacterial DD-peptidase) was inhibited by the amphiphilic glycolipid antibiotic moenomycin. Inhibition at the peptidase site was determined by competition experiments between moenomycin and the chromophoric beta-lactam nitrocefin. Under conditions of high salt ... More
Fluorescent probes of biological membranes.
AuthorsWaggoner AS, Stryer L
JournalProc Natl Acad Sci U S A
PubMed ID5289011
'FLUORESCENT AND PHOSPHORESCENT PROBES THAT HAVE READILY INTERPRETABLE EMISSION PROPERTIES CAN BE SPECIFICALLY INSERTED INTO BIOLOGICAL MACROMOLECULES TO REVEAL FACETS OF THEIR STRUCTURE AND DYNAMICS: (1) Proximity. Singlet-singlet and triplet-singlet energy transfer can serve as spectroscopic rulers in the 10-65 A range, whereas triplet-triplet transfer can be used to show ... More
Mapping the phospholipid-binding surface and translocation determinants of the C2 domain from cytosolic phospholipase A2.
AuthorsPerisic O, Paterson HF, Mosedale G, Lara-González S, Williams RL
JournalJ Biol Chem
PubMed ID10329700
'Cytosolic phospholipase A2 (cPLA2) plays a key role in the generation of arachidonic acid, a precursor of potent inflammatory mediators. Intact cPLA2 is known to translocate in a calcium-dependent manner from the cytosol to the nuclear envelope and endoplasmic reticulum. We show here that the C2 domain of cPLA2 alone ... More
Membrane binding properties of the factor IX gamma-carboxyglutamic acid-rich domain prepared by chemical synthesis.
AuthorsJacobs M, Freedman SJ, Furie BC, Furie B
JournalJ Biol Chem
PubMed ID7929250
'The fully gamma-carboxylated peptides based upon the complete and truncated Gla/aromatic amino acid stack domains of human Factor IX were prepared by solid phase peptide synthesis using Fmoc (N-(9-fluorenyl)methoxycarbonyl) chemistry. A 47-residue peptide Factor IX-(1-47) and a 42-residue peptide Factor IX-(1-42), both containing 12 residues of L-gamma-carboxyglutamic acid, were purified ... More
Polar angle as a determinant of amphipathic alpha-helix-lipid interactions: a model peptide study.
AuthorsUematsu N, Matsuzaki K
JournalBiophys J
PubMed ID11023911
'Various physicochemical properties play important roles in the membrane activities of amphipathic antimicrobial peptides. To examine the effects of the polar angle, two model peptides, thetap100 and thetap180, with polar angles of 100 degrees and 180 degrees, respectively, were designed, and their interactions with membranes were investigated in detail. These ... More
Effects of temperature and glycerides on the enhancement of Agkistrodon piscivorus piscivorus phospholipase A2 activity by lysolecithin and palmitic acid.
'The effect of temperature and various glycerides to modulate the ability of lysolecithin and fatty acid to promote high phospholipase A2 activity was studied using dipalmitoylphosphatidylcholine large unilamellar vesicles as substrate. The length of the lag phase prior to the accumulation of sufficient hydrolysis products (lysolecithin and fatty acid) to ... More
Kinetics of pore formation by an antimicrobial peptide, magainin 2, in phospholipid bilayers.
AuthorsMatsuzaki K, Murase O, Miyajima K
JournalBiochemistry
PubMed ID7548003
'The kinetics of the pore formation by magainin 2, an antimicrobial peptide from Xenopus laevis, in lipid vesicles was investigated. The pore formation was estimated by the efflux of a fluorescent dye, calcein, from large unilamellar vesicles composed of egg yolk phosphatidylglycerol. The time courses of the dye release were ... More
A simplified approach to resonance energy transfer in membranes, lipoproteins and spatially restricted systems.
'A general model is developed to simulate dipole-dipole resonance energy transfer in spatially restricted systems. At low concentrations of acceptor molecule, the overall quantum yield of a donor population can be defined quantitatively in terms of transfer to multiple defined acceptor regions. Energy transfer at higher acceptor concentrations can be ... More
Relationships between bilayer structure and phospholipase A2 activity: interactions among temperature, diacylglycerol, lysolecithin, palmitic acid, and dipalmitoylphosphatidylcholine.
AuthorsBell JD, Burnside M, Owen JA, Royall ML, Baker ML
JournalBiochemistry
PubMed ID8664287
'Bilayers composed of phosphatidylcholine initially resist catalysis by phospholipase A2. However, after a latency period, they become susceptible when sufficient reaction products (lysolecithin and fatty acid) accumulate in the membrane. Temperatures near the main bilayer phase transition and saturated long-chain diacylglycerol in the bilayer modulate the effectiveness of the reaction ... More
Characterization of the interaction of phospholipase A(2) with phosphatidylcholine-phosphatidylglycerol mixed lipids.
AuthorsGadd ME, Biltonen RL
JournalBiochemistry
PubMed ID10933778
'The first requirement in the hydrolysis of phospholipid bilayers by phospholipase A(2) is the interaction of the enzyme with the bilayer surface. The catalytic ability of phospholipase A(2) has been shown to be extremely sensitive to the topology of the bilayer to which it binds and hydrolyzes. Phospholipid bilayer properties ... More
Definition of the specific roles of lysolecithin and palmitic acid in altering the susceptibility of dipalmitoylphosphatidylcholine bilayers to phospholipase A2.
AuthorsHenshaw JB, Olsen CA, Farnbach AR, Nielson KH, Bell JD
JournalBiochemistry
PubMed ID9692961
'Bilayers composed of phosphatidylcholine initially resist catalysis by phospholipase A2. However, after a latency period, they become susceptible when sufficient reaction products (lysolecithin and fatty acid) accumulate in the membrane. Temperature near the main bilayer phase transition and calcium concentration modulate the effectiveness of the reaction products. The purpose of ... More
Dynamic behavior of fluorescent probes in lipid bilayer model membranes.
AuthorsBadley RA, Martin WG, Schneider H
JournalBiochemistry
PubMed ID4683001
Lectin binding to complex carbohydrate at the interface: a study by resonance energy transfer.
AuthorsChakrabarti A, Podder SK
JournalIndian J Biochem Biophys
PubMed ID1512011
'The binding affinity of the oligosaccharide moiety of a neutral glycosphingolipid, asialoGM1, towards Ricinus communis agglutinin (RCAI) was determined for the first time by fluorescence resonance energy transfer (RET). The asialoGM1 was incorporated into a phospholipid (DMPC) vesicle doped with dansylated DPPE and then titrated with an increasing amount of ... More
Binding of endostatin to phosphatidylserine-containing membranes and formation of amyloid-like fibers.
AuthorsZhao H, Jutila A, Nurminen T, Wickström SA, Keski-Oja J, Kinnunen PK
JournalBiochemistry
PubMed ID15723529
'Endostatin, the 20-kDa C-terminal NC1 domain of collagen XVIII, is an endogenous inhibitor of tumor angiogenesis and tumor growth. A major problem in reconciling the many reported in vitro effects of endostatin is the lack of a high-affinity receptor, and a search for the latter continues. In accordance with the ... More
Electrostatic interactions at charged lipid membranes. Measurement of surface pH with fluorescent lipoid pH indicators.
AuthorsVaz WL, Nisksch A, Jähnig F
JournalEur J Biochem
PubMed ID23947
'The 5-dimethylaminonapthalene-1-sulfonyl (dansyl) chromophore attached to the polar head groups of lipids has been used as a fluorescent lipoid pH indicator to evaluate the interfacial pH in lipid-water lamellar systems prepared from negatively charged lipids. The pH in the vicinity of the charged lipid bilayers is different from the pH ... More
Ca2+-signaling cycle of a membrane-docking C2 domain.
AuthorsNalefski EA, Slazas MM, Falke JJ
JournalBiochemistry
PubMed ID9340010
'The C2 domain is a Ca2+-dependent, membrane-targeting motif originally discovered in protein kinase C and recently identified in numerous eukaryotic signal-transducing proteins, including cytosolic phospholipase A2 (cPLA2) of the vertebrate inflammation pathway. Intracellular Ca2+ signals recruit the C2 domain of cPLA2 to cellular membranes where the enzymatic domain hydrolyzes specific ... More
The C2 domains of classical PKCs are specific PtdIns(4,5)P2-sensing domains with different affinities for membrane binding.
AuthorsGuerrero-Valero M, Marín-Vicente C, Gómez-Fernández JC, Corbalán-García S,
JournalJ Mol Biol
PubMed ID17586528
'C2 domains are conserved protein modules in many eukaryotic signaling proteins, including the protein kinase (PKCs). The C2 domains of classical PKCs bind to membranes in a Ca(2+)-dependent manner and thereby act as cellular Ca(2+) effectors. Recent findings suggest that the C2 domain of PKCalpha interacts specifically with phosphatidylinositols 4,5-bisphosphate ... More
Effects of platelet activating factor on calcium-lipid interactions and lateral phase separations in phospholipid vesicles.
AuthorsBratton DL, Harris RA, Clay KL, Henson PM
JournalBiochim Biophys Acta
PubMed ID3401478
'Recent studies localizing the inflammatory mediator, platelet activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), to the membranes of stimulated neutrophils, raise the possibility that PAF may, in addition to its activities as a mediator, alter the physical properties of membranes. This, and the increasing evidence that calcium-lipid interactions may have central importance in ... More
Mode of action of an antiviral peptide from HIV-1. Inhibition at a post-lipid mixing stage.
AuthorsKliger Y, Gallo SA, Peisajovich SG, Munoz-Barroso I, Avkin S, Blumenthal R, Shai Y
JournalJ Biol Chem
PubMed ID11027678
'DP178, a synthetic peptide corresponding to a segment of the transmembrane envelope glycoprotein (gp41) of human immunodeficiency virus, type 1 (HIV-1), is a potent inhibitor of viral infection and virus-mediated cell-cell fusion. Nevertheless, DP178 does not contain gp41 coiled-coil cavity binding residues postulated to be essential for inhibiting HIV-1 entry. ... More
Membrane permeabilization mechanisms of a cyclic antimicrobial peptide, tachyplesin I, and its linear analog.
AuthorsMatsuzaki K, Yoneyama S, Fujii N, Miyajima K, Yamada K, Kirino Y, Anzai K
JournalBiochemistry
PubMed ID9245412
'Tachyplesin I (T-SS), an antimicrobial peptide from Tachypleus tridentatus, has a cyclic antiparallel beta-sheet structure maintained by two disulfide bridges. The peptide effectively permeabilizes both bacterial and artificial lipid membranes. T-Acm, a linear analog peptide with the four SH groups protected by acetamidomethyl groups, exhibits a much weaker membrane-permeabilizing activity ... More
Kinetics of bilirubin transfer between serum albumin and membrane vesicles. Insight into the mechanism of organic anion delivery to the hepatocyte plasma membrane.
AuthorsZucker SD, Goessling W, Gollan JL
JournalJ Biol Chem
PubMed ID7836362
'Unconjugated bilirubin is transported in the plasma bound primarily to serum albumin, from which it is taken up and metabolized by the liver. To better characterize the mechanism of bilirubin delivery to the hepatocyte, stopped-flow techniques were utilized to study the kinetics of bilirubin transfer between serum albumin and both ... More
Kinetics of phosphatidylinositol-specific phospholipase C with vesicles of a thiophosphate analogue of phosphatidylinositol.
AuthorsHendrickson HS, Banovetz C, Kirsch MJ, Hendrickson EK
JournalChem Phys Lipids
PubMed ID9022217
'1,2-Dimyristoyloxypropane-3-thiophospho(1D-1-myo-inositol) (D-thio-DMPI) was synthesized as a substrate for the continuous spectrophotometric assay of phosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus cereus. Release of thio-diglyceride is followed by a coupled reaction with 4,4''-dithiopyridine to produce a chromophore, 4-thiopyridine, measured by its absorption at 324 nm. Sonicated vesicles of D-thio-DMPI gave sigmoidal Michaelis-Menten ... More
Cloning and recombinant expression of a novel mouse-secreted phospholipase A2.
AuthorsValentin E, Koduri RS, Scimeca JC, Carle G, Gelb MH, Lazdunski M, Lambeau G
JournalJ Biol Chem
PubMed ID10383420
'Secreted phospholipases A2 (sPLA2s) form a class of structurally related enzymes that are involved in a variety of physiological and pathological effects including inflammation and associated diseases, cell proliferation, cell adhesion, and cancer, and are now known to bind to specific membrane receptors. Here, we report the cloning and expression ... More
Effects of cholesterol in chylomicron remnant models of lipid emulsions on apoE-mediated uptake and cytotoxicity of macrophages.
AuthorsSakurai A, Morita SY, Wakita K, Deharu Y, Nakano M, Handa T
JournalJ Lipid Res
PubMed ID15930510
'Chylomicron remnants have been suggested to be involved in the development of atherosclerosis. To investigate the mechanisms of chylomicron remnant-induced atherosclerosis, we prepared cholesterol (Chol)-containing emulsion particles as models for chylomicron remnants. Chol markedly increased the apolipoprotein E (apoE) binding maximum of emulsions without changing the binding affinity and thereby ... More
Membrane lipid composition and vesicle size modulate bilirubin intermembrane transfer. Evidence for membrane-directed trafficking of bilirubin in the hepatocyte.
'To characterize the mechanisms underlying intracellular bilirubin transport, stopped-flow fluorometry was utilized to study the effects of membrane vesicle size and lipid composition on the kinetics of unconjugated bilirubin movement between model and native hepatocyte membranes. Bilirubin transfer rates declined asymptotically with increasing donor vesicle diameter, due primarily to a ... More
The omega-loop region of the human prothrombin gamma-carboxyglutamic acid domain penetrates anionic phospholipid membranes.
AuthorsFalls LA, Furie BC, Jacobs M, Furie B, Rigby AC
JournalJ Biol Chem
PubMed ID11312259
'The hydrophobic omega-loop within the prothrombin gamma-carboxyglutamic acid-rich (Gla) domain is important in membrane binding. The role of this region in membrane binding was investigated using a synthetic peptide, PT-(1-46)F4W, which includes the N-terminal 46 residues of human prothrombin with Phe-4 replaced by Trp providing a fluorescent probe. PT-(1-46)F4W and ... More
On the mechanism of the lipoprotein lipase-induced fluorescence changes in dansyl phosphatidylethanolamine-labeled very low density lipoproteins.
AuthorsJohnson JD, Taskinen MR, Matsuoka N, Jackson RL
JournalJ Biol Chem
PubMed ID7364750
Use of energy transfer to assay the association of proteins with lipid membranes.
AuthorsVaz WL, Kaufmann K, Nicksch A
JournalAnal Biochem
PubMed ID603032
Application of Forster resonance energy transfer to interactions between cell or lipid vesicle surfaces.
AuthorsGibson GA, Loew LM
JournalBiochem Biophys Res Commun
PubMed ID454441
Correlations between fluorescence, x-ray diffraction, and physiological properties in cytoplasmic membrane vesicles isolated from Escherichia coli.
AuthorsShechter E, Gulik-Krzywicki T, Kaback HR
JournalBiochim Biophys Acta
PubMed ID4340260
Effects of ions on vesicles and phospholipid dispersions studied by polarization of fluorescence.
AuthorsLussan C, Faucon JF
JournalBiochim Biophys Acta
PubMed ID4209039
Aliphatic chain transitions of phospholipid vesicles and phospholipid dispersions determined by polarization of fluorescence.
AuthorsFaucon JF, Lussan C
JournalBiochim Biophys Acta
PubMed ID4740471
Dynamics of lipid fluorophors in bovine lens fiber membranes.
AuthorsLi L, Spector A, Cogan U, Schachter D
JournalExp Eye Res
PubMed ID6895732
Phospholipid vesicle fusion monitored by fluorescence energy transfer.
AuthorsGibson GA, Loew LM
JournalBiochem Biophys Res Commun
PubMed ID454440
Structure, function and regulation of Na-K-ATPase.
AuthorsSchwartz A, Adams RJ, Ball WJ, Collins JH, Gupte SS, Lane LK, Reeves AS, Wallick ET
JournalInt J Biochem
PubMed ID6249660
Lipid mixing assays to determine fusion in liposome systems.
AuthorsHoekstra D, Düzgünes N
JournalMethods Enzymol
PubMed ID8350751
Surface density determination in membranes by fluorescence energy transfer.
AuthorsFung BK, Stryer L
JournalBiochemistry
PubMed ID728398
Fluorescence temperature jump relaxations of dansylphosphatidylethanolamine in aqueous dispersions of dipalmitoylphosphatidylcholine during the gel to liquid-crystal transition.
AuthorsTeissie J
JournalBiochim Biophys Acta
PubMed ID582803
The change of fluorescence of the probe dansylphosphatidylethanolamine embedded in multilamellar liposomes of dipalmitoylphosphatidylcholine was used to investigate the dynamics of the thermotropic gel to liquid-crystalline phase transition by use of the temperature-jump technique. The results are discussed and compared to published observations on the same system in which the ... More
Synaptotagmin: a calcium sensor on the synaptic vesicle surface.
AuthorsBrose N, Petrenko AG, Südhof TC, Jahn R
JournalScience
PubMed ID1589771
Neurons release neurotransmitters by calcium-dependent exocytosis of synaptic vesicles. However, the molecular steps transducing the calcium signal into membrane fusion are still an enigma. It is reported here that synaptotagmin, a highly conserved synaptic vesicle protein, binds calcium at physiological concentrations in a complex with negatively charged phospholipids. This binding ... More
A fluorescence polarization study of calcium and phase behaviour in synaptosomal lipids.
AuthorsAshley RH, Brammer MJ
JournalBiochim Biophys Acta
PubMed ID6696888
Steady-state fluorescence polarization of the fluorescent probe 1,6-diphenyl-1,3,5-hexatriene reported temperature-dependent lipid order in L-alpha-dimyristoylphosphatidylcholine, egg phosphatidylcholine and synaptosomal membranes. No change in lipid order was detected after depolarization of synaptosomes by veratridine (150 microM) even in the presence of 2 mM CaCl2. However, Ca2+ reduced the mobility of a second ... More
Single-molecule fluorescence studies of a PH domain: new insights into the membrane docking reaction.
AuthorsKnight JD, Falke JJ,
JournalBiophys J
PubMed ID19167305
Proteins containing membrane targeting domains play essential roles in many cellular signaling pathways. However, important features of the membrane-bound state are invisible to bulk methods, thereby hindering mechanistic analysis of membrane targeting reactions. Here we use total internal reflection fluorescence microscopy (TIRFM), combined with single particle tracking, to probe the ... More
Translocation of a channel-forming antimicrobial peptide, magainin 2, across lipid bilayers by forming a pore.
AuthorsMatsuzaki K, Murase O, Fujii N, Miyajima K
JournalBiochemistry
PubMed ID7538786
A channel-forming antimicrobial peptide, magainin 2, has been shown to translocate across phospholipid bilayers by forming a pore comprising multimeric peptides. The translocation was demonstrated by four sets of experiments by use of resonance energy transfer from tryptophan introduced into the peptide to a dansyl chromophore incorporated into the lipid ... More
Interaction of protein kinase C with phosphatidylserine. 2. Specificity and regulation.
AuthorsOrr JW, Newton AC
JournalBiochemistry
PubMed ID1581317
The roles of specific and nonspecific interactions in the regulation of protein kinase C by lipid have been examined. Binding and activity measurements reveal two mechanisms by which protein kinase C interacts with membranes: (1) a specific binding to the activating lipid phosphatidylserine and (2) a nonspecific binding to nonactivating, ... More
Translocation of the pAntp peptide and its amphipathic analogue AP-2AL.
AuthorsDrin G, Déméné H, Temsamani J, Brasseur R
JournalBiochemistry
PubMed ID11327845
The pAntp peptide, corresponding to the third helix of the homeodomain of the Antennapedia protein, enters by a receptor-independent process into eukaryotic cells. The interaction between the pAntp peptide and the phospholipid matrix of the plasma membrane seems to be the first step involved in the translocation mechanism. However, the ... More
Time-resolved emission spectroscopy of the dansyl fluorescence probe.
AuthorsGhiggino KP, Lee AG, Meech SR, O'Connor DV, Phillips D
JournalBiochemistry
PubMed ID6895314
Using a cavity-dumped laser system as the light source, we have recorded time-resolved fluorescence spectra for the dansyl group in a variety of systems. The fluorescence spectrum of dansylphosphatidylethanolamine incorporated into a variety of lipid bilayer systems at ambient temperatures shows very marked time dependence, with red shifts of up ... More
Measurement of ethanolamine- and serine-containing phospholipids by high-performance liquid chromatography with fluorescence detection of their Dns derivatives.
AuthorsChen SS, Kou AY, Chen HH
JournalJ Chromatogr
PubMed ID7240358
We describe a liquid chromatographic procedure for the analysis of amino group-containing phosphoglycerides in tissue. The total lipid extract is derivatized with Dns-chloride at 50 degrees C for 3 h. Dns derivatives of phosphatidylethanolamine, lysophosphatidylethanolamine, phosphatidylserine and lysophosphatidylserine are separated by a silica gel column with gradient elution. The eluate ... More
Fluorescence quenching measurements of the membrane bound lipid haptens with different length spacers.
AuthorsKimura K, Arata Y, Yasuda T, Kinosita K, Nakanishi M
JournalBiochim Biophys Acta
PubMed ID1550857
Dansyl lipid haptens with three different length (short, intermediate and long) spacers have been incorporated into DMPC or DPPC liposomes. Anti-DNS-IgG bound most efficiently to these liposomes containing lipid haptens with an intermediate length spacer, although the binding efficiency became more increased when liposomes were made of a mixture of ... More
Transbilayer transport of ions and lipids coupled with mastoparan X translocation.
AuthorsMatsuzaki K, Yoneyama S, Murase O, Miyajima K
JournalBiochemistry
PubMed ID8679603
The transbilayer movement of ions and lipids induced by mastoparan X, a peptidic toxin from Vespa xanthoptera, was investigated by use of lipid vesicles as a model membrane system. Negatively charged phosphatidylglycerol remarkably enhanced the peptide-lipid interactions. Mastoparan X induced the ion flow by forming a short-lived, multimeric pore in ... More
Alpha-crystallin/lens lipid interactions using resonance energy transfer.
AuthorsTang D, Borchman D, Yappert MC
JournalOphthalmic Res
PubMed ID10474075
Resonance energy transfer was used to study the interaction of alpha-crystallin with lens cortex lipid vesicles. The binding of alpha-crystallin to cortex lipid vesicles and the preincubation temperature dependence of the binding were confirmed. In this study, the tryptophan of alpha-crystallin was used as the energy donor, and the fluorescence ... More
Dansyl phosphatidylethanolamine-labeled very low density lipoproteins. A fluorescent probe for monitoring lipolysis.
AuthorsJohnson JD, Taskinen MR, Matsuoka N, Jackson RL
JournalJ Biol Chem
PubMed ID6892706
The fluorescent phospholipid dansyl phosphatidylethanolamine (DPE) (dansyl, 5-dimethylaminonaphthalene-1-sulfonyl) was incorporated into very low density lipoproteins (VLDL) to form DPE-VLDL. The addition of milk lipoprotein lipase to DPE-VLDL in the presence of albumin resulted in a greater than 3-fold fluorescence increase and a 20 nm blue shift in the wavelength of ... More
Dynamics of binary mixtures of plasmenylcholine/arachidonic acid and phosphatidylcholine/arachidonic acid--a study using fluorescence and NMR spectroscopy.
AuthorsChen X, Han X, Gross RW
JournalBiochim Biophys Acta
PubMed ID8323943
Arachidonic acid is selectively released during signal transduction in many cell types. To examine the effects of physiologically relevant amounts of arachidonic acid on membrane bilayers, alterations in membrane dynamics induced by arachidonic acid were investigated utilizing fluorescence and nuclear magnetic resonance spectroscopy. We demonstrate that decreases in the fluorescence ... More
Membrane-embedded synaptotagmin penetrates cis or trans target membranes and clusters via a novel mechanism.
AuthorsBai J, Earles CA, Lewis JL, Chapman ER
JournalJ Biol Chem
PubMed ID10840045
The synaptic vesicle protein synaptotagmin I has been proposed to serve as a Ca(2+) sensor for rapid exocytosis. Synaptotagmin spans the vesicle membrane once and possesses a cytoplasmic domain largely comprised of two C2 domains designated C2A and C2B. We have determined how deep the Ca(2+)-binding loops of Ca(2+).C2A penetrate ... More
Interaction of cationic antimicrobial peptides with model membranes.
AuthorsZhang L, Rozek A, Hancock RE
JournalJ Biol Chem
PubMed ID11473117
A series of natural and synthetic cationic antimicrobial peptides from various structural classes, including alpha-helical, beta-sheet, extended, and cyclic, were examined for their ability to interact with model membranes, assessing penetration of phospholipid monolayers and induction of lipid flip-flop, membrane leakiness, and peptide translocation across the bilayer of large unilamellar ... More
Lipid mixing during freeze-thawing of liposomal membranes as monitored by fluorescence energy transfer.
AuthorsMacDonald RI, MacDonald RC
JournalBiochim Biophys Acta
PubMed ID6688739
A new pair of fluorescence-energy-transferring probes, dansylphosphatidylethanolamine and dioctadecylindocarbocyanine, were incorporated separately into phospholipid vesicles to monitor intervesicle lipid mixing under various conditions. The transfer efficiencies of mixtures of sonicated vesicles labeled with 2 wt% donor dansylphosphatidylethanolamine (DnsPE) or with 1 wt% acceptor dioctadecylindocarbocyanine (DiI-C18) were negligible, but increased to ... More
Pentraxin family of proteins interact specifically with phosphorylcholine and/or phosphorylethanolamine.
AuthorsSchwalbe RA, Dahlbäck B, Coe JE, Nelsestuen GL
JournalBiochemistry
PubMed ID1375509
Pentraxins are a family of serum proteins characterized by five identical subunits that are noncovalently linked. The two major types of pentraxins are C-reactive protein (CRP) and serum amyloid P component (SAP). CRP proteins are identified by their calcium-dependent interaction with phosphorylcholine. This study showed that SAP also bound to ... More
Rapid transbilayer movement of the fluorescent sterol dehydroergosterol in lipid membranes.
AuthorsJohn K, Kubelt J, Müller P, Wüstner D, Herrmann A
JournalBiophys J
PubMed ID12202377
This study establishes a new assay for measuring the transbilayer movement of dehydroergosterol (DHE) in lipid membranes. The assay is based on the rapid extraction of DHE by methyl-beta-cyclodextrin (M-CD) from liposomes. The concentration of DHE in the liposomal membrane was measured by using fluorescence resonance energy transfer (FRET) from ... More
Hydration and molecular motions in synthetic phytanyl-chained glycolipid vesicle membranes.
AuthorsBaba T, Minamikawa H, Hato M, Handa T
JournalBiophys J
PubMed ID11721000
Proton permeation rates across membranes of a synthetic branch-chained glycolipid, 1,3-di-O-phytanyl-2-O-(beta-D-maltotriosyl)glycerol (Mal3(Phyt)2) as well as a branch-chained phospholipid, diphytanoylphosphatidylcholine (DPhPC) were lower than those of straight-chained lipids such as egg yolk phosphatidylcholine (EPC) by a factor of approximately 4 at pH 7.0 and 25 degrees C. To examine whether degrees ... More
Properties of palmitoyl phosphatidylcholine, sphingomyelin, and dihydrosphingomyelin bilayer membranes as reported by different fluorescent reporter molecules.
AuthorsNyholm T, Nylund M, Söderholm A, Slotte JP
JournalBiophys J
PubMed ID12547780
The properties of vesicle membranes prepared from 16:0-SM, 16:0-DHSM, or DPPC were characterized using steady-state and time-resolved fluorescence spectroscopy and different fluorescent reporter molecules. The acyl-chain region was probed using free and phospholipid-bound 1,6-diphenyl-1,3,5-hexatriene. 16:0-DHSM was found to be the more ordered than both DPPC and 16:0-SM 5 degrees C ... More
Fluorescence-based assays of lipases, phospholipases, and other lipolytic enzymes.
AuthorsHendrickson HS
JournalAnal Biochem
PubMed ID8059934
In choosing an assay, one needs to consider the following questions: What level of sensitivity is required? Must the assay be continuous? Is the substrate readily available; can it be purchased or must it be custom synthesized? How specific is the substrate? How convenient is the method? How compatible is ... More
Uncovering a calcium-regulated membrane-binding mechanism for soybean lipoxygenase-1.
AuthorsTatulian SA, Steczko J, Minor W
JournalBiochemistry
PubMed ID9799511
Lipoxygenases catalyze the biosynthesis of leukotrienes, lipoxins, and other lipid-derived mediators that are involved in a wide variety of pathophysiological processes, including inflammation, allergy, and tumorigenesis. Mammalian lipoxygenases are activated by a calcium-mediated translocation to intracellular membranes upon cell stimulation, and cooperate with cytosolic phospholipase A2 at the membrane surface ... More
Unconjugated bilirubin exhibits spontaneous diffusion through model lipid bilayers and native hepatocyte membranes.
AuthorsZucker SD, Goessling W, Hoppin AG
JournalJ Biol Chem
PubMed ID10196162
The liver is responsible for the clearance and metabolism of unconjugated bilirubin, the hydrophobic end-product of heme catabolism. Although several putative bilirubin transporters have been described, it has been alternatively proposed that bilirubin enters the hepatocyte by passive diffusion through the plasma membrane. In order to elucidate the mechanism of ... More
Differing reactivities of human and guinea-pig complement on haptenized liposomes via the alternative pathway.
AuthorsOkada N, Yasuda T, Tsumita T, Okada H
JournalMol Immunol
PubMed ID6194429
Several haptens coupled to dipalmitoylphosphatidylethanolamine (DPPE) were inserted into the liposome membrane with a base composition of an equimolecular mixture of dimyristoylphosphatidylcholine (DMPC) and cholesterol (Chol). Haptens used were trinitrophenyl (TNP)-DPPE, TNP-aminocaproyl (TNP-Cap)-DPPE, dinitrophenyl (DNP)-DPPE, DNP-aminocaproyl (DNP-Cap)-DPPE, fluoresceinthiocarbamyl (Fl)-DPPE, azobenzenarsonate-tyrosyl (ABA-Tyr)-DPPE, dansyl (DNS)-DPPE, dabsyl (DABS)-DPPE, dithiopyridyl (DTP)-DPPE and maleimidobenzoyl (MB)-DPPE. ... More
Role of phosphatidylethanolamine in assembly and function of the factor IXa-factor VIIIa complex on membrane surfaces.
AuthorsFalls LA, Furie B, Furie BC
JournalBiochemistry
PubMed ID11052674
Phospholipid membranes play a significant role during the proteolytic activation of blood coagulation proteins. This investigation identifies a role for phosphatidylethanolamine (PE) during the activation of factor X by the tenase complex, an enzymatic complex composed of the serine protease, factor IXa, a protein cofactor, factor VIIIa, a phospholipid membrane, ... More
Mechanism of synergism between antimicrobial peptides magainin 2 and PGLa.
AuthorsMatsuzaki K, Mitani Y, Akada KY, Murase O, Yoneyama S, Zasloff M, Miyajima K
JournalBiochemistry
PubMed ID9790678
The antimicrobial peptides magainin 2 and PGLa, discovered in the skin of the African clawed frog, Xenopus laevis, exhibit marked synergism [Westerhoff, H. V., Zasloff, M., Rosner, J. L., Hendler, R. W., de Waal, A., Vaz Gomes, A., Jongsma, A. P. M., Riethorst, A., and Juretic, D., Eur. J. Biochem. ... More
Modulation of magainin 2-lipid bilayer interactions by peptide charge.
AuthorsMatsuzaki K, Nakamura A, Murase O, Sugishita K, Fujii N, Miyajima K
JournalBiochemistry
PubMed ID9047309
Magainin 2, an antimicrobial peptide from Xenopus skin, assumes an amphiphilic helix when bound to acidic phospholipids, forming a pore composed of a dynamic, peptide-lipid supramolecular complex [Matsuzaki et al. (1996) Biochemistry 35, 11361-11368]. Upon the disintegration of the pore, a fraction of the peptide molecules stochastically translocates across the ... More
Apolipoproteins C-I, C-II, and C-III: kinetics of association with model membranes and intermembrane transfer.
The apoproteins (apo) C-I, C-II, and C-III are low molecular weight amphiphilic proteins that are associated with the lipid surface of the plasma chylomicron, very low density lipoprotein (VLDL), and high-density lipoprotein (HDL) subfractions. Purified apoC-I spontaneously reassociates with VLDL, HDL, and single-bilayer vesicles (SBV) of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine. ApoC-I also transfers ... More
Interfacial activation of triglyceride lipase from Thermomyces (Humicola) lanuginosa: kinetic parameters and a basis for control of the lid.
A strategy is developed to analyze steady-state kinetics for the hydrolysis of a soluble substrate partitioned into the interface by an enzyme at the interface. The feasibility of this approach to obtain interfacial primary kinetic and equilibrium parameters is demonstrated for a triglyceride lipase. Analysis for phospholipase A2 catalyzed hydrolysis ... More
Lipid headgroup spacing and peptide penetration, but not peptide oligomerization, modulate peptide-induced fusion.
AuthorsPécheur EI, Sainte-Marie J, Bienvenüe A, Hoekstra D
JournalBiochemistry
PubMed ID9890918
In this study, the mechanism by which an amphipathic negatively charged peptide consisting of 11 amino acids (WAE) induces fusion of liposomal phosphatidylcholine membranes is investigated. WAE-induced fusion, which only occurs when the peptide is covalently attached to the bilayer, shows a highly remarkable dependence on naturally occurring phosphatidylcholine species. ... More
Interactions of the novel antimicrobial peptide buforin 2 with lipid bilayers: proline as a translocation promoting factor.
AuthorsKobayashi S, Takeshima K, Park CB, Kim SC, Matsuzaki K
JournalBiochemistry
PubMed ID10913273
Buforin 2 is an antimicrobial peptide discovered in the stomach tissue of the Asian toad Bufo bufo gargarizans. The 21-residue peptide with +6 net charge shows antimicrobial activity an order of magnitude higher than that of magainin 2, a membrane-permeabilizing antimicrobial peptide from Xenopus laevis [Park, C. B., Kim, M. ... More
Location of the membrane-docking face on the Ca2+-activated C2 domain of cytosolic phospholipase A2.
AuthorsNalefski EA, Falke JJ
JournalBiochemistry
PubMed ID9922129
Docking of C2 domains to target membranes is initiated by the binding of multiple Ca2+ ions to a conserved array of residues imbedded within three otherwise variable Ca2+-binding loops. We have located the membrane-docking surface on the Ca2+-activated C2 domain of cPLA2 by engineering a single cysteine substitution at 16 ... More
Synergistic activation of protein kinase Calpha, -betaI, and -gamma isoforms induced by diacylglycerol and phorbol ester: roles of membrane association and activating conformational changes.
AuthorsSlater SJ, Milano SK, Stagliano BA, Gergich KJ, Ho C, Mazurek A, Taddeo FJ, Kelly MB, Yeager MD, Stubbs CD
JournalBiochemistry
PubMed ID10090770
Protein kinase Calpha (PKCalpha) has been shown to contain two discrete activator sites with differing binding affinities for phorbol esters and diacylglycerols. The interaction of diacylglycerol with a low-affinity phorbol ester binding site leads to enhanced high-affinity phorbol ester binding and to a potentiated level of activity [Slater, S. J., ... More
Calcium is not required for 5-lipoxygenase activity at high phosphatidyl choline vesicle concentrations.
AuthorsSkorey KI, Gresser MJ
JournalBiochemistry
PubMed ID9609696
5-Lipoxygenase (5-LO) catalyzes the formation of 5-hydroperoxy-eicosatetraenoic acid (5-HPETE) and leukotriene A4 (LTA4) from arachidonic acid. Following a rise in intracellular calcium, 5-LO translocates to a membrane where it reacts with arachidonic acid via an 18 kD protein (FLAP). In vitro studies using a vesicle system of phosphatidylcholine (PC) and ... More
Membrane binding kinetics of factor VIII indicate a complex binding process.
AuthorsBardelle C, Furie B, Furie BC, Gilbert GE
JournalJ Biol Chem
PubMed ID8473326
Factor VIII functions as a component of the tenase enzyme complex upon phospholipid membranes. Factor VIII binds to phosphatidylserine-containing membranes and apparently provides high affinity binding sites for factor IXa upon these membranes. We have characterized the binding kinetics of human factor VIII with phosphatidylserine-containing membranes and directly compared the ... More
C2 domains from different Ca2+ signaling pathways display functional and mechanistic diversity.
The ubiquitous C2 domain is a conserved Ca2+ triggered membrane-docking module that targets numerous signaling proteins to membrane surfaces where they regulate diverse processes critical for cell signaling. In this study, we quantitatively compared the equilibrium and kinetic parameters of C2 domains isolated from three functionally distinct signaling proteins: cytosolic ... More
Ionic properties of membrane association by vitamin K-dependent proteins: the case for univalency.
AuthorsMcDonald JF, Evans TC, Emeagwali DB, Hariharan M, Allewell NM, Pusey ML, Shah AM, Nelsestuen GL
JournalBiochemistry
PubMed ID9398287
Ionic properties of membrane interaction by prothrombin, protein Z, and other vitamin K-dependent proteins were studied to determine the relevance of a monovalent membrane contact mechanism between one phospholipid headgroup and a calcium-lined pore in the protein [McDonald, J. F., Shah, A. M., Schwalbe, R. A., Kisiel, W., Dahlback, B., ... More
Inhibition of phospholipase C-delta 1 catalytic activity by sphingomyelin.
We measured the ability of sphingomyelin (SPM) to inhibit phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] hydrolysis catalyzed by human phospholipase C-delta 1 (PLC-delta 1) in model membranes and detergent phospholipid mixed micelles. SPM strongly inhibited PLC-delta 1 catalytic activity measured in large unilamellar vesicles (LUVs) composed of egg phosphatidylcholine (PC), PI(4,5)P2, and SPM ... More
Ca(2+)-dependent binding of endonexin (annexin IV) to membranes: analysis of the effects of membrane lipid composition and development of a predictive model for the binding interaction.
AuthorsJunker M, Creutz CE
JournalBiochemistry
PubMed ID8043580
Endonexin (annexin IV) is a member of the annexin family of homologous proteins that bind membranes and aggregate vesicles in a calcium-dependent fashion. This study examines the lipid modulation and mechanism of the binding of endonexin to membranes using a fluorescence energy transfer assay to measure bovine endonexin binding to ... More
Membrane binding kinetics of protein kinase C betaII mediated by the C2 domain.
AuthorsNalefski EA, Newton AC
JournalBiochemistry
PubMed ID11683630
Conventional isoforms of protein kinase C (PKC) are activated when their two membrane-targeting modules, the C1 and C2 domains, bind the second messengers diacylglycerol (DG) and Ca2+, respectively. This study investigates the mechanism of Ca2+-induced binding of PKC betaII to anionic membranes mediated by the C2 domain. Stopped-flow fluorescence spectroscopy ... More
Low- and high-affinity phorbol ester and diglyceride interactions with protein kinase C: 1-O-alkyl-2-acyl-sn-glycerol enhances phorbol ester- and diacylglycerol-induced activity but alone does not induce activity.
AuthorsSlater SJ, Seiz JL, Stagliano BA, Cook AC, Milano SK, Ho C, Stubbs CD
JournalBiochemistry
PubMed ID11352745
Phorbol ester-induced conventional protein kinase C (PKCalpha, -betaIota/IotaIota, and -gamma) isozyme activities are potentiated by 1,2-diacyl-sn-glycerol. This has been attributed to a "cooperative" interaction of the two activators with two discrete sites termed the low- and high-affinity phorbol ester binding sites, respectively [Slater, S. J., Milano, S. K., Stagliano, B. ... More
Importance of cis-proline 22 in the membrane-binding conformation of bovine prothrombin.
AuthorsEvans TC, Nelsestuen GL
JournalBiochemistry
PubMed ID8679575
Upon addition of calcium to the metal-free protein, bovine prothrombin displays a conformational change with behavior of a classic trans- to cis-proline isomerization. The change is accompanied by a decrease of the intrinsic protein fluorescence and is essential to creating the membrane-binding conformation of prothrombin. This study showed that an ... More
Calcium-triggered selective intermembrane exchange of phospholipids by the lung surfactant protein SP-A.
AuthorsCajal Y, Dodia C, Fisher AB, Jain MK
JournalBiochemistry
PubMed ID9724531
It is shown that human lung surfactant protein (SP-A) mediates selective exchange of phospholipid probes with unlabeled phospholipid in excess vesicles in the presence of calcium and NaCl. The exchange occurs without leakage of vesicle contents, or transbilayer movement (flip-flop) of the phospholipid probes, or fusion of vesicles. Individual steps ... More
Fluorescent probes of membrane surface properties.
AuthorsEpand RF, Kraayenhof R, Sterk GJ, Wong Fong Sang HW, Epand RM
JournalBiochim Biophys Acta
PubMed ID8914583
We have studied the properties of two new fluorescent probes, 7-dimethylaminocoumarin derivatives, 4-[N, N-dimethyl-N-(n-tetradecyl)ammoniummethyl]-7-(N,N-dimethylamino)co umarin chloride (TAMAC) and 4-(n-dodecylthiomethyl)-7-(N,N-dimethylamino)coumarin (DTMAC) in model membrane systems. Both probes are sensitive to solvent polarity. The TAMAC probe has a quaternary ammonium function to position it at a fixed location with respect to the ... More
Binding of steroidogenic acute regulatory protein to synthetic membranes suggests an active molten globule.
AuthorsChristensen K, Bose HS, Harris FM, Miller WL, Bell JD
JournalJ Biol Chem
PubMed ID11279152
Steroidogenic acute regulatory protein (StAR) mediates cholesterol transport from the outer to the inner mitochondrial membrane during steroid biosynthesis. The mechanism of StAR's action is not established. To address mechanistic issues, we assessed the binding of StAR to artificial membranes by fluorescence resonance energy transfer using endogenous StAR tryptophan residues ... More
Hydration and order in lipid bilayers.
AuthorsHo C, Slater SJ, Stubbs CD
JournalBiochemistry
PubMed ID7742324
The relationship between membrane lipid bilayer hydration and acyl chain order was investigated using time-resolved fluorescence spectroscopy. The degree of hydration in the head group region was assessed from fluorescence lifetime data along with fluorescence intensity measurements in D2O, relative to H2O buffer, using N-(5-dimethylaminonaphthalene-1-sulfonyl)dipalmitoylphosphatidylethan ola mine (dansyl-PE). The degree ... More
Binding of recombinant rat liver fatty acid-binding protein to small anionic phospholipid vesicles results in ligand release: a model for interfacial binding and fatty acid targeting.
AuthorsDavies JK, Thumser AE, Wilton DC
JournalBiochemistry
PubMed ID10606528
A number of intracellular proteins bind to negatively charged phospholipid membranes, and this interfacial binding results in a conformational change that modulates the activity of the protein. Using a fluorescent fatty acid analogue, 11-[5-(dimethylamino)naphthalenesulfonyl]undecanoic acid (DAUDA), it is possible to demonstrate the release of this ligand from recombinant rat liver ... More
Calcium and membrane-binding properties of monomeric and multimeric annexin II.
AuthorsEvans TC, Nelsestuen GL
JournalBiochemistry
PubMed ID7947730
The calcium-dependent interaction of several annexins with membranes was studied. A novel technique was developed that allowed estimation of calcium binding to aggregating systems. This consisted of immobilized phospholipids on phenyl-Sepharose. Proteins associated with this affinity gel in the presence of radioactive calcium and were eluted with the same buffer ... More