Functional tests for the characterization of surfactant protein B (SP-B) and a fluorescent SP-B analog.
AuthorsDiemel RV, Bader D, Walch M, Hotter B, van Golde LM, Amann A, Haagsman HP, Putz G
JournalArch Biochem Biophys
PubMed ID11368015
Surfactant protein B (SP-B) enhances lipid insertion into the alveolar air/liquid interface upon inhalation. The aim of this study was (i) to apply a palette of tests for a detailed biochemical and biophysical characterization of SP-B and (ii) to use these tests to compare native SP-B with a fluorescent (Bodipy) ... More