Toxicity of organic fluorophores used in molecular imaging: literature review.
AuthorsAlford R, Simpson HM, Duberman J, Hill GC, Ogawa M, Regino C, Kobayashi H, Choyke PL,
JournalMol Imaging
PubMed ID20003892
'Fluorophores are potentially useful for in vivo cancer diagnosis. Using relatively inexpensive and portable equipment, optical imaging with fluorophores permits real-time detection of cancer. However, fluorophores can be toxic and must be investigated before they can be administered safely to patients. A review of published literature on the toxicity of ... More
Novel fluorogenic substrates for acid phosphatase.
AuthorsGee KR
JournalBioorg Med Chem Lett
PubMed ID10360743
Fluorinated versions of fluorescein diphosphate (FDP) can provide significantly enhanced fluorescence upon hydrolysis by acid phosphatase, as compared with FDP, when measured at the reaction pH. ... More
Fluorescent indicators for intracellular pH.
AuthorsHan J, Burgess K,
JournalChem Rev
PubMed ID19831417
This review is about intracellular pH sensors, includingsmall fluorescent organic molecules, nanoparticles, andfluorescent proteins, e.g., GFP. It focuses on their preparations, photophysical properties, and advantages/disadvantagesfor intracellular pH measurements. The discussion is limitedto fluorescent indicators that have been applied to measureintracellular pH values since 1980. ... More
Exposure of cells to hydrogen peroxide can increase the intracellular accumulation of drugs.
AuthorsFunk RS, Krise JP,
JournalMol Pharm
PubMed ID17274672
One of the fastest growing areas of scientific research involves aspects of oxidative stress, either causes of or results from. Despite the enormous quantity of literature on the topic, surprisingly, the effects of oxidative stress on the pharmacokinetics of drugs have not been previously investigated. This is an extremely important ... More
Two phases of zymogen granule lifetime in mouse pancreas: ghost granules linger after exocytosis of contents.
AuthorsThorn P, Parker I
JournalJ Physiol
PubMed ID15637100
Different cell types show widely divergent mechanisms and kinetics of exocytosis. We investigated these processes in pancreatic acinar cells by using video-rate 2-photon microscopy to image entry of extracellular dye into individual zymogen granules undergoing exocytosis. Fluorescence signals display two distinct phases; an initial peak that then decays over several ... More
2',7'-Difluorofluorescein excited-state proton reactions: correlation between time-resolved emission and steady-state fluorescence intensity.
AuthorsOrte A, Bermejo R, Talavera EM, Crovetto L, Alvarez-Pez JM
JournalJ Phys Chem A
PubMed ID16833599
The presence of excited-state buffer-mediated proton exchange reactions influences the steady-state fluorescence signals from dyes in solution. Since biomolecules in general have some chemical groups that can act as proton acceptors/donors and are usually dissolved in buffer solutions which can also behave as appropriate proton acceptors/donors, the excited-state proton exchange ... More
Three-state 2',7'-difluorofluorescein excited-state proton transfer reactions in moderately acidic and very acidic media.
AuthorsOrte A, Talavera EM, Maçanita AL, Orte JC, Alvarez-Pez JM
JournalJ Phys Chem A
PubMed ID16834272
2',7'-Difluorofluorescein (Oregon Green 488, OG488) is a novel fluorescein dye derivative which presents important advantages for improving the fluorimetric applications in the biomedical and biochemical sciences. In aqueous solution it displays four prototropic forms, namely cation (C), neutral (N), monoanion (M), and dianion (D). In previous works, we found (J. ... More
Absorption and emission study of 2',7'-difluorofluorescein and its excited-state buffer-mediated proton exchange reactions.
AuthorsOrte A, Crovetto L, Talavera EM, Boens N, Alvarez-Pez JM
JournalJ Phys Chem A
PubMed ID16838941
2',7'-Difluorofluorescein (Oregon Green 488) is a new fluorescein-based dye, which has found many applications, above all in biochemistry and neurosciences, and its use has become very popular in the last years. In recent years, we have been investigating the excited-state proton exchange reactions of fluorescein and the effect of suitable ... More
Effect of capillary efflux transport inhibition on the determination of probe recovery during in vivo microdialysis in the brain.
AuthorsSun H, Bungay PM, Elmquist WF
JournalJ Pharmacol Exp Ther
PubMed ID11356921
Intracerebral microdialysis probe recovery (extraction fraction) may be influenced by several mass transport processes in the brain, including efflux and uptake exchange between brain and blood. Therefore, changes in probe recovery under various experimental conditions can be useful to characterize fundamental drug transport processes. Accordingly, the effect of inhibiting transport ... More