Cellular drug resistance, which involves several mechanisms such as P-glycoprotein (P-gp) overexpression, kinetic and metabolic quiescence, or the increase in the intracellular levels of glutathione, limits the effectiveness of cancer treatment. It has been reported that functional assessment of the cationic dye rhodamin 123 (Rho123) efflux reveals accurately the drug-resistant ... More
Flow cytometric measurement of the production of reactive oxygen intermediate in activated rat mast cells.
AuthorsTsinkalovsky OR, Laerum OD
JournalAPMIS
PubMed ID7520710
The production of reactive oxygen intermediates (ROI) in compound 48/80- or calcium ionophore A 23187- activated pleural or peritoneal mast cells was monitored using flow cytometry and the fluorescence indicator dihydrorhodamine 123 (a derivate of rhodamine 123). Mast cell degranulation and ROI production were estimated by flow cytometric fluorescence and ... More
A microtiter plate assay for screening antioxidant activity in extracts of marine organisms.
AuthorsDunlap W, Llewellyn L, Doyle J, Yamamoto Y
JournalMar Biotechnol (NY)
PubMed ID14502401
A novel microtiter plate assay was developed to determine the total peroxyl radical-trapping activity of antioxidants extracted from marine organisms by measuring the inhibition rate of dye-substrate oxidation. We compared use of dihydrorhodamine-123, dihydrofluorescein, and dichlorodihydrofluorescein as reduced substrates for oxidation by peroxyl radicals generated from 2,2'-azobis(2-amidinopropane) dihydrochloride. The oxidation ... More
Direct real-time evaluation of nitration with green fluorescent protein in solution and within human cells reveals the impact of nitrogen dioxide vs. peroxynitrite mechanisms.
AuthorsEspey MG, Xavier S, Thomas DD, Miranda KM, Wink DA
JournalProc Natl Acad Sci U S A
PubMed ID11904413
'3-Nitrotyrosyl adducts in proteins have been detected in a wide range of diseases. The mechanisms by which reactive nitrogen oxide species may impede protein function through nitration were examined by using a unique model system, which exploits a critical tyrosyl residue in the fluorophoric pocket of recombinant green fluorescent protein ... More
Rapid immune activation by CpG motifs in bacterial DNA. Systemic induction of IL-6 transcription through an antioxidant-sensitive pathway.
AuthorsYi AK, Klinman DM, Martin TL, Matson S, Krieg AM
JournalJ Immunol
PubMed ID8955187
'Unmethylated CpG dinucleotides (CpG motif) in bacterial DNA or synthetic oligodeoxynucleotides (CpG DNA) rapidly activate murine B cells to secrete IL-6 and IgM, as well as to proliferate. Within 30 min after CpG DNA stimulation in vivo, IL-6 mRNA levels were increased in liver, spleen, and thymus cells. Serum IL-6 ... More
Interaction between rose bengal and different protein components.
AuthorsTseng SC, Zhang SH
JournalCornea
PubMed ID7545566
'Bindings of rose bengal to several proteins were determined by Sephadex G-75 chromatography. Their respective blocking effect against dye uptake was demonstrated in an assay using a rabbit corneal epithelial cell layer. The total binding capacity of nonmucin proteins was measured using fluorometry and Scatchard analysis. The results showed that ... More
Human umbilical cord blood-derived eosinophils cultured in the presence of IL-3 and IL-5 respond to fMLP with [Ca2+]i variation and O2- production.
AuthorsZardini DM, Heuschling P, Gallois A, Bueb JL, Tschirhart EJ
JournalJ Immunol Methods
PubMed ID9236909
'In the presence of interleukin-3 and interleukin-5, eosinophil precursors from human umbilical cord blood mononuclear cells were regularly differentiated into mature eosinophil-like cells expressing normal morphology and cyanide-resistant peroxidase. O2- production and [Ca2+]i rise were measured in these in vitro differentiated eosinophils after fMLP stimulation; with dihydrorhodamine-123 and fura-2, respectively. ... More
Ultraviolet action spectra for peroxide generation in human and pig epidermal keratinocytes loaded with dihydrorhodamine 123.
AuthorsTakano JI, Koizumi H, Ohkawara A, Kamo N, Ueda T
JournalArch Dermatol Res
PubMed ID7598537
'We developed a new and simple method for measuring peroxides in a single living cell, and the generation of peroxides upon ultraviolet (UV) irradiation was measured in human and pig epidermal keratinocytes. The method was based on the fact that the non-fluorescent dye, dihydrorhodamine 123, reacts in the presence of ... More
Single-cell measurement of superoxide anion and hydrogen peroxide production by human neutrophils with digital imaging fluorescence microscopy.
AuthorsSzucs S, Vámosi G, Póka R, Sárváry A, Bárdos H, Balázs M, Kappelmayer J, Tóth L, Szöllosi J, Adány R
JournalCytometry
PubMed ID9725555
'Besides flow cytometry, fluorescence microscopy combined with computerized image analysis offers an alternative tool for assessing phagocyte oxidant generation at the single-cell level. This technique provides an opportunity for the direct visualization of cells and simultaneous measurement of cellular fluorescence intensity. Thus, we developed a simple method for the quantitative ... More
Activation of the respiratory burst in human eosinophils by chemotaxins requires intracellular calcium fluxes.
AuthorsElsner J, Dichmann S, Kapp A
JournalJ Invest Dermatol
PubMed ID7636306
'Eosinophils represent major effector cells in the allergic inflammatory response. Following activation, these cells are capable of mediating tissue damage, particularly by the release of reactive oxygen species. In this study, the role of extracellular and intracellular calcium in the induction of the respiratory burst of human eosinophils was investigated ... More
The mitochondrial network of human neutrophils: role in chemotaxis, phagocytosis, respiratory burst activation, and commitment to apoptosis.
AuthorsFossati G, Moulding DA, Spiller DG, Moots RJ, White MR, Edwards SW
JournalJ Immunol
PubMed ID12574365
'It is commonly assumed that human neutrophils possess few, if any, functional mitochondria and that they do not depend on these organelles for cell function. We have used the fluorescent mitochondrial indicators, JC-1, MitoTracker Red, and dihydrorhodamine 123 to show that live neutrophils possess a complex mitochondrial network that extends ... More
Increased intracellular generation of reactive oxygen species in mononuclear leukocytes from patients with diabetes mellitus type 2.
AuthorsOrie NN, Zidek W, Tepel M
JournalExp Clin Endocrinol Diabetes
PubMed ID10926312
'Since increased cellular production of reactive oxygen species is a source of oxidative stress and thus may contribute to the development of diabetic complications, the baseline and stimulated concentrations of intracellular reactive oxygen species were measured in 16 patients with diabetes mellitus type 2 and 19 healthy control subjects. Reactive ... More
Cellular features of an apoptotic form of programmed cell death during the development of the ascidian, Boltenia villosa.
AuthorsBates WR
JournalZoolog Sci
PubMed ID15170059
'The phylogenetic position of ascidians near the base of the chordate tree makes them ideal organisms for evolutionary developmental studies of programmed cell death (PCD). In the present study, the following key features of an apoptotic form of PCD are described in Boltenia villosa: fragmentation of DNA, increases in plasma ... More
Oxidative stress during platelet-activating factor-induced microvascular dysfunction.
AuthorsKurose I, Argenbright LW, Wolf R, Granger DN
JournalMicrocirculation
PubMed ID9086452
'OBJECTIVE: To assess the potential contribution of hydrogen peroxide (H2O2) to the leukocyte-endothelial cell adhesion and increased microvascular permeability (to fluorescein isothiocyanate [FITC]-albumin) observed in rat mesenteric venules exposed to platelet-activating factor (PAF). METHODS: The production of oxidants derived from H2O2 in mesenteric tissue was monitored using the H2O2-sensitive fluorochrome, ... More
Unique oxidative mechanisms for the reactive nitrogen oxide species, nitroxyl anion.
AuthorsMiranda KM, Espey MG, Yamada K, Krishna M, Ludwick N, Kim S, Jourd'heuil D, Grisham MB, Feelisch M, Fukuto JM, Wink DA
JournalJ Biol Chem
PubMed ID11042174
'The nitroxyl anion (NO-) is a highly reactive molecule that may be involved in pathophysiological actions associated with increased formation of reactive nitrogen oxide species. Angeli''s salt (Na2N2O3; AS) is a NO- donor that has been shown to exert marked cytotoxicity. However, its decomposition intermediates have not been well characterized. ... More
The protective role of endogenous melatonin in carrageenan-induced pleurisy in the rat.
AuthorsCuzzocrea S, Tan DX, Costantino G, Mazzon E, Caputi AP, Reiter RJ
JournalFASEB J
PubMed ID10544176
'Peroxynitrite, a potent cytotoxic oxidant formed by the reaction of nitric oxide (NO) with the superoxide anion, was recently proposed to play a major pathogenic role in the inflammatory process. Here we have investigated the effects of endogenous melatonin, a known scavenger of peroxynitrite, in rats subjected to carrageenan-induced pleurisy. ... More
Divergence of mechanisms regulating respiratory burst in blood and sputum eosinophils and neutrophils from atopic subjects.
AuthorsLacy P, Abdel-Latif D, Steward M, Musat-Marcu S, Man SF, Moqbel R
JournalJ Immunol
PubMed ID12594296
'Eosinophil respiratory burst is an important event in asthma and related inflammatory disorders. However, little is known concerning activation of the respiratory burst NADPH oxidase in human eosinophils. Conversely, neutrophils are known to assemble NADPH oxidase in intracellular and plasma membranes. We hypothesized that eosinophils and neutrophils translocate NADPH oxidase ... More
Mild intraoperative hypothermia reduces production of reactive oxygen intermediates by polymorphonuclear leukocytes.
AuthorsWenisch C, Narzt E, Sessler DI, Parschalk B, Lenhardt R, Kurz A, Graninger W
JournalAnesth Analg
PubMed ID8615502
'Mild hypothermia directly impairs numerous immune functions in vitro. However, the in vivo effects of mild hypothermia on neutrophil phagocytosis and oxidative killing remain unknown. We tested the hypothesis that mild intraoperative hypothermia decreases neutrophil phagocytic capacity and generation of reactive oxygen intermediates (a measure of oxidative killing). Additionally, we ... More
A Fenton reaction at the endoplasmic reticulum is involved in the redox control of hypoxia-inducible gene expression.
AuthorsLiu Q, Berchner-Pfannschmidt U, Möller U, Brecht M, Wotzlaw C, Acker H, Jungermann K, Kietzmann T
JournalProc Natl Acad Sci U S A
PubMed ID15010533
'It has been proposed that hydroxyl radicals (.OH) generated in a perinuclear iron-dependent Fenton reaction are involved in O(2)-dependent gene expression. Thus, it was the aim of this study to localize the cellular compartment in which the Fenton reaction takes place and to determine whether scavenging of.OH can modulate hypoxia-inducible ... More
Effects of nicaraven on nitric oxide-related pathways and in shock and inflammation.
AuthorsZingarelli B, Scott GS, Hake P, Salzman AL, Szabo C
JournalShock
PubMed ID10670842
'Expression of the inducible isoform of nitric oxide (NO) synthase, and the formation of peroxynitrite from NO and superoxide are responsible for some of the pathophysiological alterations seen during reperfusion injury and in various inflammatory conditions. Some of the effects of peroxynitrite are related to DNA single-strand breakage, and activation ... More
The population of bipolar cells in the rabbit retina.
AuthorsMacNeil MA, Heussy JK, Dacheux RF, Raviola E, Masland RH
JournalJ Comp Neurol
PubMed ID15024753
'The population of bipolar cells in the rabbit retina was studied using Golgi impregnation and photocatalyzed filling of single cells with dihydrorhodamine, a quantitative sampling technique. The Golgi method revealed the morphology and stratification of cells in detail. The photofilling method allowed us to estimate the frequency of the cell ... More
Effects of atrial natriuretic peptide on phagocytosis and respiratory burst in murine macrophages.
AuthorsVollmar AM, Förster R, Schulz R
JournalEur J Pharmacol
PubMed ID9042602
'Atrial natriuretic peptide (ANP) is known to affect cardiovascular physiology displaying both hormone- and neurotransmitter characteristics. However, there is increasing evidence that ANP possesses additional biological activities referring to the immune system. To further strengthen this hypothesis the effect of ANP on two major functions of macrophages, i.e., phagocytosis and ... More
UHMWPE oxidation increases granulocytes activation: a role in tissue response after prosthesis implant.
AuthorsRenò F, Lombardi F, Cannas M
JournalBiomaterials
PubMed ID12742728
'Ultra-high molecular weight polyethylene (UHMWPE), a biopolymer widely used in orthopaedic implants, is oxidized during gamma-ray sterilization; such surface oxidation is considered as major responsible for inflammation and prosthesis failure. As granulocytes are involved in first contact inflammation, we have measured their oxidative burst by flow cytometry using dihydrorhodamine 123 ... More
Peroxynitrite-mediated oxidation of dihydrorhodamine 123.
'Nitric oxide reacts with superoxide to form peroxynitrite, which may be an important mediator of free radical-induced cellular injury. Oxidation of dihydrorhodamine to fluorescent rhodamine is a marker of cellular oxidant production. We investigated the mechanisms of peroxynitrite-mediated formation of rhodamine from dihydrorhodamine. Peroxynitrite at low levels (0-1000 nM) induced ... More
Flow-cytometric analysis of oxidative and proteolytical activities in tissue-associated phagocytes from normal and hypertrophic muscles.
AuthorsLöhrke B, Wegner J, Viergutz T, Dietl G, Ender K
JournalAnal Cell Pathol
PubMed ID8616104
'The study was conducted by the known tendencies of increased stress-susceptibility and metabolic disorders in individuals with hypertrophied muscles due to innate factors or intensive exercise which can induce the overtraining syndrome. Using an animal model, muscle-associated cells from normal (N) and hypertrophic (H) skeletal muscle (m.semitendinosus) were examined in ... More
Active oxidants mediate IFN-alpha-induced microvascular alterations in rat mesentery.
AuthorsHiguchi H, Kurose I, Fukumura D, Yan HJ, Saito H, Miura S, Hokari R, Watanabe N, Zeki S, Yoshida M, Kitajima M, Granger DN, Ishii H
JournalJ Immunol
PubMed ID9144506
'The present study was designed to investigate the influences of IFN-alpha on the microcirculatory hemodynamics. The mesenteric microcirculation of male Wistar rats was observed through an intravital fluorescence microscopic system. The leukocyte behavior, RBC velocity, and albumin leakage were monitored simultaneously before and after a continuous infusion of IFN-alpha. In ... More
Severe phenotype of chronic granulomatous disease presenting in a female with a de novo mutation in gp91-phox and a non familial, extremely skewed X chromosome inactivation.
AuthorsAnderson-Cohen M, Holland SM, Kuhns DB, Fleisher TA, Ding L, Brenner S, Malech HL, Roesler J
JournalClin Immunol
PubMed ID14697745
'Chronic granulomatous disease (CGD) is an inherited immunodeficiency resulting from defects in the multienzyme complex NADPH-oxidase (phagozyte oxidase, phox), which normally produces microbicidal reactive oxygen metabolites (ROM). The reason for our patient''s CGD was unusual, as revealed by the following in vitro findings in neutrophils and EBV-transformed B-cells: lack of ... More
The use of N-t-butyl hydroxylamine for radioprotection in cultured cells and mice.
AuthorsLee JH, Kim IS, Park JW,
JournalCarcinogenesis
PubMed ID15016661
'Exposure of cells to ionizing radiation leads to formation of reactive oxygen species (ROS) that are associated with radiation-induced cytotoxicity. Therefore, compounds that scavenge ROS may confer radioprotective effects. Recently, it has been shown that the decomposition product of the spin-trapping agent alpha-phenyl-N-t-butylnitrone (PBN), N-t-butyl hydroxylamine (NtBHA), mimics PBN and ... More
Local hypothermia during early reperfusion protects skeletal muscle from ischemia-reperfusion injury.
AuthorsMowlavi A, Neumeister MW, Wilhelmi BJ, Song YH, Suchy H, Russell RC
JournalPlast Reconstr Surg
PubMed ID12496585
'Amputated tissue maintained in a hypothermic environment can endure prolonged ischemia and improve replantation success. The authors hypothesized that local tissue hypothermia during the early reperfusion period may provide a protective effect against ischemia-reperfusion injury similar to that seen when hypothermia is provided during the ischemic period. A rat gracilis ... More
Participation of reactive oxygen species in the lysophosphatidic acid-stimulated mitogen-activated protein kinase kinase activation pathway.
AuthorsChen Q, Olashaw N, Wu J
JournalJ Biol Chem
PubMed ID7499358
'Recent evidence suggests that reactive oxygen species (ROS) may function as second messengers in intracellular signal transduction pathways. We explored the possibility that ROS were involved in lysophosphatidic acid (LPA)-induced mitogen-activated protein (MAP) kinase signaling pathway in HeLa cells. Antioxidant N-acetylcysteine inhibited the LPA-stimulated MAP kinase kinase activity. Direct exposure ... More
Beneficial effects of 3-aminobenzamide, an inhibitor of poly (ADP-ribose) synthetase in a rat model of splanchnic artery occlusion and reperfusion.
AuthorsCuzzocrea S, Zingarelli B, Costantino G, Szabó A, Salzman AL, Caputi AP, Szabó C
JournalBr J Pharmacol
PubMed ID9249240
'1. Peroxynitrite, a potent cytotoxic oxidant formed by the reaction of nitric oxide with superoxide anion, and hydroxyl radical, formed in the iron-catalysed Fenton reaction, are important mediators of reperfusion injury. In in vitro studies, DNA single strand breakage, triggered by peroxynitrite or by hydroxyl radical, activates the nuclear enzyme ... More
Quercetin modifies reactive oxygen levels but exerts only partial protection against oxidative stress within HL-60 cells.
AuthorsBestwick CS, Milne L
JournalBiochim Biophys Acta
PubMed ID11514098
'Quercetin may contribute to the protection afforded by fruit- and vegetable-rich diets against diseases for which excess production of reactive oxygen species (ROS) has been implicated as a causal or contributory factor. We examine the effect of short term (90 min) quercetin (1-100 microM) exposure on the progress of menadione ... More
[Morphine inhibits complement receptor expression, phagocytosis and oxidative burst by a nitric oxide dependent mechanism]
AuthorsMenzebach A, Hirsch J, Nöst R, Mogk M, Hempelmann G, Welters ID
'OBJECTIVE: Monocytes play a crucial role in the immune response by recognition, ingestion, and intracellular killing of microorganisms. We investigated whether morphine and fentanyl influence CD 11b and CD35 surface receptor expression, phagocytic activity and superoxide anion generation of monocytes in a whole blood flow cytometric assay. METHODS: Whole blood ... More
Pivotal role of mitochondrial calcium uptake in neural cell apoptosis and necrosis.
AuthorsKruman II, Mattson MP
JournalJ Neurochem
PubMed ID9930724
'Perturbed cellular calcium homeostasis has been implicated in both apoptosis and necrosis, but the role of altered mitochondrial calcium handling in the cell death process is unclear. The temporal ordering of changes in cytoplasmic ([Ca2+]C) and intramitochondrial ([Ca2+]M) calcium levels in relation to mitochondrial reactive oxygen species (ROS) accumulation and ... More
Beneficial effects of melatonin in a rat model of splanchnic artery occlusion and reperfusion.
AuthorsCuzzocrea S, Costantino G, Mazzon E, Micali A, De Sarro A, Caputi AP
JournalJ Pineal Res
PubMed ID10626602
'The aim of the present study was to investigate the protective effect of the pineal secretary product melatonin in a model of splanchnic artery occlusion shock (SAO). SAO shock was induced in rats by clamping both the superior mesenteric artery and the celiac trunk for 45 min, followed thereafter by ... More
Reversibility of leukocyte dysfunction in rats with obstructive jaundice.
AuthorsLi W, Sung JJ, Chung SC
JournalJ Surg Res
PubMed ID15013371
'BACKGROUND: The role of leukocytes in obstructive jaundice is obscure and the effect of relieving cholestasis on leukocyte function is unclear. We postulated that cholestasis affects systemic polymorphonuclear leukocyte function by deranging phagocytosis and hydrogen peroxide release and the leukocyte dysfunction is reversible by internal and external biliary drainage. MATERIALS ... More
Labile plasma iron in iron overload: redox activity and susceptibility to chelation.
'Plasma non-transferrin-bound-iron (NTBI) is believed to be responsible for catalyzing the formation of reactive radicals in the circulation of iron overloaded subjects, resulting in accumulation of oxidation products. We assessed the redox active component of NTBI in the plasma of healthy and beta-thalassemic patients. The labile plasma iron (LPI) was ... More
Modulation of intracellular formation of reactive oxygen intermediates in peritoneal macrophages and microglia/brain macrophages by propentofylline.
'Ischemia-induced nerve cell death can partly be prevented by propentofylline, a pharmacon structurally related to xanthine derivates that interacts with the neuromodulatory function of endogenous adenosine. To evaluate a possible mechanism of neuroprotection by propentofylline, we studied its effect on the cellular production of reactive oxygen intermediates in microglial cells, ... More
Attenuation of acute lung injury and oxygen radical production by the 21-aminosteroid, U-78518F.
AuthorsTanigaki T, Suzuki Y, Heimer D, Sussman HH, Ross WG, Raffin TA
JournalJ Appl Physiol
PubMed ID8335543
'Oxygen radicals play an important role in the mechanism of acute lung injury. The 21-aminosteroid lazaroid, U-78518F, is a potent antioxidant. We examined the effect of intravenous U-78518F on acute lung injury in septic guinea pigs over 8 h. The experimental groups (n = 6) were 1) saline control, 2) ... More
Production of reactive oxygen species by hemocytes from the marine mussel, Mytilus edulis: lysosomal localization and effect of xenobiotics.
AuthorsWinston GW, Moore MN, Kirchin MA, Soverchia C
JournalComp Biochem Physiol C Pharmacol Toxicol Endocrinol
PubMed ID8646615
'Hemolymph of M. Edulis is rich in phagocytic hemocytes. Hemocytes contain numerous lysosomes which, in turn, contain various hydrolytic enzymes. Phagocytic activity of M. edulis hemocytes is thought to be associated with NAD(P)H-oxidase activity of the plasma membrane. The laser dye, dihydrorhodamine 123 (DHR), was used for cytochemical and biochemical ... More
Threshold and graded response behavior in human neutrophils: effect of varying G-protein or ligand concentrations.
AuthorsRiccobene TA, Waller A, Hoffman JF, Linderman JJ, Omann GM
JournalBiochemistry
PubMed ID9708989
'Observing the qualitative characteristics of response behavior as key variables in the signal transduction cascade are changed can provide insight into the fundamental roles of these interactions in producing cellular responses. Using flow cytometric assays and pertussis toxin (PT) treatment of human neutrophils, we have shown that actin polymerization stimulated ... More
In vivo visualization of reactive oxidants and leukocyte-endothelial adherence following hemorrhagic shock.
'The generation of oxygen radicals during leukocyte-endothelial cell interaction is considered to represent one of the fundamental steps of microvascular injury following ischemia and reperfusion. Indirect evidence also suggests that this relationship may be important following hemorrhagic shock. The purpose of this study was to characterize the temporal changes of ... More
Neuronal formation of free radicals plays a minor role in hypoxic cell death in human NT2-N neurons.
AuthorsAlmaas R, Saugstad OD, Pleasure D, Rootwelt T
JournalPediatr Res
PubMed ID11809906
'Free radicals are suggested to play an important role in hypoxic-ischemic neuronal death. However, the importance in human disease is not known. Furthermore, whether posthypoxic free radical formation mainly occurs in endothelium and neutrophils, or whether neuronal production is important, is not finally determined. To study this we differentiated human ... More
DNA damage induced by tumour necrosis factor-alpha in L929 cells is mediated by mitochondrial oxygen radical formation.
AuthorsShoji Y, Uedono Y, Ishikura H, Takeyama N, Tanaka T
JournalImmunology
PubMed ID7790027
'Treatment of L929 cells with tumour necrosis factor-alpha (TNF-alpha) plus actinomycin D induced DNA damage (indicated by the appearance of a sub-G1 peak due to extracellular leakage of low molecular weight DNA following DNA fragmentation) before significant cell lysis occurred. The DNA damage occurred in parallel with a decrease of ... More
Anti-neutrophil cytoplasmic antibodies engage and activate human neutrophils via Fc gamma RIIa.
AuthorsPorges AJ, Redecha PB, Kimberly WT, Csernok E, Gross WL, Kimberly RP
JournalJ Immunol
PubMed ID8027554
'The presence of anti-neutrophil cytoplasmic Abs (ANCA) in many patients with systemic vasculitis suggests that ANCA may play a role in disease pathogenesis. Neutrophils from patients with Wegener''s granulomatosis often express ANCA target Ags (myeloperoxidase (MPO) and proteinase 3 (PR3)) on their surface, making these intracellular primary granule enzymes accessible ... More
Survival of FimH-expressing enterobacteria in macrophages relies on glycolipid traffic.
AuthorsBaorto DM, Gao Z, Malaviya R, Dustin ML, van der Merwe A, Lublin DM, Abraham SN
JournalNature
PubMed ID9335508
'Strains of Escherichia coli persist within the human gut as normal commensals, but are frequent pathogens and can cause recurrent infection. Here we show that, in contrast to E. coli subjected to opsonic interactions stimulated by the host''s immune response, E. coli that bind to the macrophage surface exclusively through ... More
Prospective randomized comparison of cefodizime versus cefuroxime for perioperative prophylaxis in patients undergoing coronary artery bypass grafting.
AuthorsWenisch C, Bartunek A, Zedtwitz-Liebenstein K, Hiesmayr M, Parschalk B, Pernerstorfer T, Graninger W
JournalAntimicrob Agents Chemother
PubMed ID9210690
'The effects of cefodizime and cefuroxime on neutrophil phagocytosis and reactive oxygen production in 54 patients undergoing elective coronary artery bypass grafting were studied. Both drugs were administered twice at a dosage of 40 mg/kg of body weight (pre- and intraoperative). Phagocytic capacity was assessed by measuring the uptake of ... More
Flow cytometric quantitation of human opsonin-dependent phagocytosis and oxidative burst responses to meningococcal antigens.
AuthorsLehmann AK, Halstensen A, Bassøe CF
JournalCytometry
PubMed ID9845434
'A one-step flow cytometric (FCM) assay has been developed to quantify both opsonin- and antigen-dependent phagocytosis and intraphagocyte oxidative burst responses. Meningococcal outer membrane structures (OMV) were adsorbed to fluorescent polystyrene beads, opsonized with serum, and exposed to leukocytes. FCM parameters of phagocytosis were evaluated in combinations with oxidative burst ... More
Effects of cocaine and its oxidative metabolites on mitochondrial respiration and generation of reactive oxygen species.
AuthorsBoess F, Ndikum-Moffor FM, Boelsterli UA, Roberts SM
JournalBiochem Pharmacol
PubMed ID10927019
'Cocaine is capable of producing severe hepatocellular necrosis in laboratory animals and humans. The mechanism of cocaine hepatotoxicity is not well understood, but appears to result from the actions of one or more N-oxidative metabolites of cocaine. Mitochondria have been proposed as critical cellular targets for cocaine toxicity, and previous ... More
Dihydrorhodamine 123: a fluorescent probe for superoxide generation?
AuthorsHenderson LM, Chappell JB
JournalEur J Biochem
PubMed ID8223655
'Imaging techniques, such as confocal microscopy and fluorescent activated cells scan are facilitating the study of responses at the single-cell level. Superoxide is reported to oxidise the non-fluorescent dihydrorhodamine 123 (DHR) to rhodamine 123. The generation of rhodamine 123 by human neutrophils, stimulated by the phorbol ester phorbol 12-myristate 13-acetate ... More
Mechanism of clofibrate hepatotoxicity: mitochondrial damage and oxidative stress in hepatocytes.
AuthorsQu B, Li QT, Wong KP, Tan TM, Halliwell B
JournalFree Radic Biol Med
PubMed ID11522451
'Peroxisome proliferators have been found to induce hepatocarcinogenesis in rodents, and may cause mitochondrial damage. Consistent with this, clofibrate increased hepatic mitochondrial oxidative DNA and protein damage in mice. The present investigation aimed to study the mechanism by which this might occur by examining the effect of clofibrate on freshly ... More
Expression of Id1 results in apoptosis of cardiac myocytes through a redox-dependent mechanism.
'We have constructed a recombinant adenovirus (Ad.Id1) that allows for efficient expression of the helix-loop-helix protein Id1. After infection with Ad.Id1, neonatal cardiac myocytes display a significant reduction in viability, which was proportional to the level of Id1 expression. A similar effect was observed in adult myocytes. Morphological and biochemical ... More
Lymphocyte migration through monolayers of endothelial cell lines involves VCAM-1 signaling via endothelial cell NADPH oxidase.
AuthorsMatheny HE, Deem TL, Cook-Mills JM
JournalJ Immunol
PubMed ID10843714
'Lymphocytes migrate from the blood across endothelial cells to reach foreign substances sequestered in peripheral lymphoid organs and inflammatory sites. To study intracellular signaling in endothelial cells during lymphocyte migration, we used murine endothelial cell lines that promote lymphocyte migration and constitutively express VCAM-1. The maximum rate of resting splenic ... More
Peroxynitrite mediates IL-8 gene expression and production in lipopolysaccharide-stimulated human whole blood.
AuthorsFilep JG, Beauchamp M, Baron C, Paquette Y
JournalJ Immunol
PubMed ID9820546
'Recent evidence indicates that free oxygen radicals, in particular hydroxyl radicals, may act as intracellular second messengers for the induction of IL-8, a potent chemoattractant and activator of neutrophil granulocytes. Here we report that peroxynitrite (ONOO-), formed by a reaction of nitric oxide (NO) with superoxide, mediates IL-8 gene expression ... More
New silicon compounds as resistance modifiers against multidrug-resistant cancer cells.
AuthorsMolnar J, Mucsi I, Nacsa J, Hevér A, Gyémánt N, Ugocsai K, Hegyes P, Kiessig S, Gaal D, Lage H, Varga A
JournalAnticancer Res
PubMed ID15161039
'The efficiency of chemotherapy is often decreased by the development of resistance of cancer cells to cytostatic drugs. This phenomenon is in most cases caused by the activity of the various ABC transporters, multidrug-resistance (MDR) gene-encoded p-glycoproteins, that pump anticancer drugs out of the cells. The inhibition of the activities ... More
Overexpression of PHGPx inhibits hydroperoxide-induced oxidation, NFkappaB activation and apoptosis and affects oxLDL-mediated proliferation of rabbit aortic smooth muscle cells.
'Rabbit abdominal aortic smooth muscle cells (SMC) were stably transfected with the cDNA of porcine phospholipid hydroperoxide glutathione peroxidase (PHGPx) by means of a retroviral gene transfer technique, to create a model for studying cellular processes relevant to atherogenesis. The transfected cells (SMC/PHGPx) had approximately 4-fold higher PHGPx activity when ... More
Stimulation of the pentose phosphate pathway and glutathione levels by dehydroascorbate, the oxidized form of vitamin C.
AuthorsPuskas F, Gergely P, Banki K, Perl A
JournalFASEB J
PubMed ID10877828
'Ascorbic acid, or vitamin C, generally functions as an antioxidant by directly reacting with reactive oxygen intermediates and has a vital role in defenses against oxidative stress. However, ascorbic acid also has pro-oxidant properties and may cause apoptosis of lymphoid and myeloid cells. The present study shows that dehydroascorbate, the ... More
Further characterization of the neutrophil oxidative burst by flow cytometry.
AuthorsSmith JA, Weidemann MJ
JournalJ Immunol Methods
PubMed ID8315293
'The ability to generate reactive oxygen species is essential for neutrophils to kill infectious micro-organisms. We have investigated the ''oxidative burst'' in human neutrophils stimulated in vitro with either opsonized zymosan (OZ) or phorbol myristate acetate (PMA) at the single cell level by flow cytometry using dichlorofluorescein diacetate (DCFH-DA) and ... More
Mechanism of oxidative stress from low levels of carbon monoxide.
AuthorsThom SR, Ischiropoulos H
JournalRes Rep Health Eff Inst
PubMed ID9476263
'The purpose of this study was to determine whether platelets and vascular endothelial cells would liberate nitric oxide free radical (NO)* and NO-derived oxidant species after exposure to carbon monoxide (CO) at concentrations up to 100 parts per million (ppm). We hypothesized that exposure to environmentally relevant concentrations of CO ... More
Burn-induced lung damage in rat is mediated by a nitric oxide/cGMP system.
AuthorsChen LW, Hwang YC, Chen CJ, Wang JS, Chen JS, Hsu CM
JournalShock
PubMed ID14501952
'This study was conducted to demonstrate the burn-induced lung neutrophil deposition and damage in rats is affected by the nitric oxide (NO)-dependent downstream cGMP signaling. In experiment 1, 1H-[1,2,4] oxadiazolo [4,3-alpha] quinoxalin-1-one (ODQ) was given (20 mg/kg i.p.) to specific pathogen-free Sprague-Dawley rats immediately postburn to suppress the guanylate cyclase ... More
Molecular ordering in HIV-induced apoptosis. Oxidative stress, activation of caspases, and cell survival are regulated by transaldolase.
AuthorsBanki K, Hutter E, Gonchoroff NJ, Perl A
JournalJ Biol Chem
PubMed ID9565623
'Dysregulated apoptosis may underlie the etiology of T cell depletion by human immunodeficiency virus type 1 (HIV-1). We show that HIV-induced apoptosis is preceded by an exponential increase in reactive oxygen intermediates (ROIs) produced in mitochondria. This leads to caspase-3 activation, phosphatidylserine (PS) externalization, and GSH depletion. Since mitochondrial ROI ... More
Perfluorocarbon emulsion improves cerebral oxygenation and mitochondrial function after fluid percussion brain injury in rats.
AuthorsDaugherty WP, Levasseur JE, Sun D, Spiess BD, Bullock MR
JournalNeurosurgery
PubMed ID15113478
'OBJECTIVE: Cerebral ischemia is a common secondary sequela of traumatic brain injury (TBI). Experimental models of stroke have demonstrated reductions in ischemia after perfluorocarbon (PFC) administration; however, there are no published reports of PFC efficacy after TBI. The current study analyzed the effect of the PFC emulsion Oxygent (AF0144; Alliance ... More
Lipoic acid protects efficiently only against a specific form of peroxynitrite-induced damage.
AuthorsRezk BM, Haenen GR, van der Vijgh WJ, Bast A
JournalJ Biol Chem
PubMed ID14701855
'The ability of the sulfur-containing compounds glutathione (GSH), glutathione disulphide (GSSG), S-methylglutathione (GSMe), lipoic acid (LA), and dihydrolipoic acid (DHLA) to protect against hypochlorous acid (HOCl)-mediated damage and peroxynitrite (ONOOH)-induced damage has been compared. Protective activity was assessed in competition assays by monitoring several detectors, i.e. dihydrorhodamine-123 (DHR-123) oxidation, alpha(1)-antiproteinase ... More
PCB 104-induced proinflammatory reactions in human vascular endothelial cells: relationship to cancer metastasis and atherogenesis.
AuthorsChoi W, Eum SY, Lee YW, Hennig B, Robertson LW, Toborek M
JournalToxicol Sci
PubMed ID12805654
'Polychlorinated biphenyls (PCBs) are widespread environmental contaminants that are known to induce carcinogenic and possibly atherogenic events. Recent evidence suggests that selected PCBs may be potent developmental agents of vascular inflammatory responses by inducing cellular oxidative stress and activating redox-responsive transcription factors. Therefore, the aim of this paper is to ... More
Protective effect of melatonin on cellular energy depletion mediated by peroxynitrite and poly (ADP-ribose) synthetase activation in a non-septic shock model induced by zymosan in the rat.
AuthorsCuzzocrea S, Costantino G, Caputi AP
JournalJ Pineal Res
PubMed ID9755028
'DNA single-strand breakage and activation of the nuclear enzyme poly (ADP-ribose) synthetase (PARS) triggers an energy-consuming, inefficient repair cycle, which contributes to peroxynitrite-induced cellular injury. Recently it was proposed that zymosan, a non-bacterial agent, causes cellular injury by inducing the production of peroxynitrite and consequent PARS activation. Here we investigated ... More
Resistance of macrophages to Mycobacterium avium is induced by alpha2-adrenergic stimulation.
AuthorsWeatherby KE, Zwilling BS, Lafuse WP
JournalInfect Immun
PubMed ID12496145
'The ability of macrophages to control the growth of microorganisms is increased by macrophage activation. Previously, it was shown that epinephrine activated mouse macrophages to resist the growth of Mycobacterium avium via alpha(2)-adrenergic stimulation. In the present study, we show that the alpha(2)-adrenergic agonist (alpha(2)-agonist) clonidine induced resistance to M. ... More
Lupus erythematosus tumidus and chronic discoid lupus erythematosus in carriers of X-linked chronic granulomatous disease.
AuthorsRupec RA, Petropoulou T, Belohradsky BH, Walchner M, Liese JG, Plewing G, Messer G
JournalEur J Dermatol
PubMed ID10725815
'Two Caucasian carriers for chronic granulomatous disease (CGD) developed cutaneous lupus erythematosus (LE) with clinically and morphologically characteristic appearance for chronic discoid lupus erythematosus (DLE) and lupus erythematosus tumidus (LET). Direct immunofluorescent examinations and ANA titers were positive in both young women. No systemic involvement due to the ACR criteria ... More
Chemotactic 5-oxo-eicosatetraenoic acids induce oxygen radical production, Ca2+-mobilization, and actin reorganization in human eosinophils via a pertussis toxin-sensitive G-protein.
AuthorsCzech W, Barbisch M, Tenscher K, Schöpf E, Schröder JM, Norgauer J
JournalJ Invest Dermatol
PubMed ID8980298
'The arachidonic acid metabolites 5-oxo-[6E,8Z,11Z,14Z]-eicosatetraen oic acid (5oETE) and 5-oxo-15-hydroxy-[6E,8Z,11Z,13E]-eicosatetrae noi c acid (5oHETE) are potent eosinophil chemotaxins. Here, the activation profile of 5-oxo-eicosanoids in eosinophils was further characterized and compared to other eosinophil activators such as complement fragment C5a (C5a), platelet-activating factor (PAF), interleukin-5 (IL-5), and phorbol ester (PMA). ... More
Influence of 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase inhibitors on endothelial nitric oxide synthase and the formation of oxidants in the vasculature.
AuthorsParker RA, Huang Q, Tesfamariam B
JournalAtherosclerosis
PubMed ID12860247
'HMGCoA reductase inhibitors (statins) can have effects outside the target tissue, liver, including serious side-effects such as rhabdomyolysis as well as beneficial pleiotrophic effects. One such effect is upregulation of endothelial nitric oxide synthase (e-NOS) which generally leads to vasorelaxation. However, changing the balance between localized NO and O(2-) fluxes ... More
Relative effects of glucose and glutamine on reactive oxygen intermediate production by neutrophils.
AuthorsFurukawa S, Saito H, Matsuda T, Inoue T, Fukatsu K, Han I, Ikeda S, Hidemura A, Muto T
JournalShock
PubMed ID10774615
'The energy source for neutrophils (PMNs) has long been believed to be glucose. However, it has been shown recently that PMNs use glutamine as well as glucose. Nevertheless, the comparative effects of glucose and glutamine on PMN function remain to be clarified. This study investigated the relative effects of glucose ... More
Cerebral microvascular responses to hypercholesterolemia: roles of NADPH oxidase and P-selectin.
AuthorsIshikawa M, Stokes KY, Zhang JH, Nanda A, Granger DN
JournalCirc Res
PubMed ID14670846
'Although hypercholesterolemia is widely accepted as a major risk factor for coronary artery and peripheral vascular diseases, its role in the pathogenesis of stroke is controversial. The objectives of this study were to determine how hypercholesterolemia affects the cerebral microcirculation under resting conditions and after ischemia-reperfusion (I/R). Platelet- and leukocyte-endothelial ... More
Mitochondrial complex I, aconitase, and succinate dehydrogenase during hypoxia-reoxygenation: modulation of enzyme activities by MnSOD.
AuthorsPowell CS, Jackson RM
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID12665464
'Both NADH dehydrogenase (complex I) and aconitase are inactivated partially in vitro by superoxide (O2-.) and other oxidants that cause loss of iron from enzyme cubane (4Fe-4S) centers. We tested whether hypoxia-reoxygenation (H-R) by itself would decrease lung epithelial cell NADH dehydrogenase, aconitase, and succinate dehydrogenase (SDH) activities and whether ... More
A noninvasive fluorimetric procedure for measurement of membrane potential. Quantification of the NADPH oxidase-induced depolarization in activated neutrophils.
AuthorsJankowski A, Grinstein S
JournalJ Biol Chem
PubMed ID10473559
'The electrogenic activity of the NADPH oxidase is associated with depolarization of the plasma membrane in activated neutrophils. The magnitude and consequences of this depolarization, however, remain unknown. Neutrophils are not amenable to electrophysiological determinations of membrane potential by current clamp. Instead, the occurrence of depolarization has been inferred from ... More
Ras proteins induce senescence by altering the intracellular levels of reactive oxygen species.
AuthorsLee AC, Fenster BE, Ito H, Takeda K, Bae NS, Hirai T, Yu ZX, Ferrans VJ, Howard BH, Finkel T
JournalJ Biol Chem
PubMed ID10075689
'Human diploid fibroblasts eventually lose the capacity to replicate in culture and enter a viable but nonproliferative state of senescence. Recently, it has been demonstrated that retroviral-mediated gene transfer into primary fibroblasts of an activated ras gene (V12ras) rapidly accelerates development of the senescent phenotype. Using this in vitro system, ... More
Role of interleukin 12 in hypercholesterolemia-induced inflammation.
'We have previously shown that T lymphocytes and interferon-gamma are involved in hypercholesterolemia-induced leukocyte adhesion to vascular endothelium. This study assessed the contribution of interleukin 12 (IL-12) to these hypercholesterolemia-induced inflammatory responses. Intravital videomicroscopy was used to quantify leukocyte adhesion and emigration and oxidant stress (dihydrorhodamine oxidation) in unstimulated cremasteric ... More
H2O2 is an important mediator of UVB-induced EGF-receptor phosphorylation in cultured keratinocytes.
AuthorsPeus D, Vasa RA, Meves A, Pott M, Beyerle A, Squillace K, Pittelkow MR
JournalJ Invest Dermatol
PubMed ID9620307
'Exposure of human keratinocytes to physiologic doses of ultraviolet B (UVB) radiation induces phosphorylation of the epidermal growth factor receptor (EGFR). We demonstrate that H2O2 generated by UVB mediates EGFR phosphorylation. Using dihydrorhodamine 123 as a specific fluorescent dye probe, we show that UVB irradiation (50-800 J per m2) of ... More
A sensitive flow cytometric method for measuring the oxidative burst.
AuthorsModel MA, KuKuruga MA, Todd RF
JournalJ Immunol Methods
PubMed ID9107299
'We report a novel method of flow cytometric detection of the oxidative burst in human neutrophils. The cells were covalently labeled on the plasma membrane by incubation with 4-carboxydihydrotetramethylrosamine succinimidyl ester on ice. Activation of neutrophils resulted in an increase in red fluorescence at 575 nm using 514 nm excitation. ... More
The use of flow cytometry to measure neutrophil function.
Authorsvan Eeden SF, Klut ME, Walker BA, Hogg JC
JournalJ Immunol Methods
PubMed ID10618507
'Neutrophils are important professional phagocytic cells that provide the host with a first line of defense against acute bacterial and fungal diseases and recurrent, severe or unusual infections are associated with inherited defects of neutrophil function. Furthermore, abundant evidence links inappropriate neutrophil-mediated tissue damage to the pathogenesis of conditions such ... More
Flow cytometric evaluation of leukocyte function in rat whole blood.
AuthorsLi W, Chung SC
JournalIn Vitro Cell Dev Biol Anim
PubMed ID14733609
'The aim of this study was to establish a standard flow cytometric method to measure the phagocytic function of and intracellular hydrogen peroxide (H2O2) production by rat leukocytes. Thirty-six adult, male Sprague-Dawley rats were included in this study. Whole-blood specimens from the inferior vena cava were collected in a heparinized ... More
Structure/Function analysis of p55 tumor necrosis factor receptor and fas-associated death domain. Effect on necrosis in L929sA cells.
AuthorsBoone E, Vanden Berghe T, Van Loo G, De Wilde G, De Wael N, Vercammen D, Fiers W, Haegeman G, Vandenabeele P
JournalJ Biol Chem
PubMed ID10988295
'Tumor necrosis factor (TNF) induces a typical apoptotic cell death program in various cell lines by interacting with the p55 tumor necrosis factor receptor (TNF-R55). In contrast, triggering of the fibrosarcoma cell line L929sA gives rise to characteristic cellular changes resulting in necrosis. The intracellular domain of TNF-R55 can be ... More
Effects of stress-related signal molecules on cells associated with muscle tissue.
AuthorsLoehrke B, Renne U, Viergutz T, Ender K, Krüger B
JournalAnal Quant Cytol Histol
PubMed ID8908310
'OBJECTIVE: To elucidate the physiologic background that makes muscle hypertrophy, especially that due to strenuous exercise, often parallel to stress sensitivity and signs of acute phase immune response. STUDY DESIGN: We used an animal model: lines of mice with hypertrophied (H) and normally developed (N) hind leg muscles, six in ... More
Peroxynitrite-mediated DNA strand breakage activates poly (ADP-ribose) synthetase and causes cellular energy depletion in carrageenan-induced pleurisy.
AuthorsCuzzocrea S, Caputi AP, Zingarelli B
JournalImmunology
PubMed ID9536124
'The aim of the present study was to investigate the role of poly (ADP-ribose) synthetase in acute local inflammation (carrageenan-induced pleurisy), where oxyradicals, nitric oxide and peroxynitrite are known to play a crucial role in the inflammatory process. DNA single-strand breakage and activation of the nuclear enzyme poly (ADP-ribose) synthetase ... More
Protective effect of N-acetylcysteine on cellular energy depletion in a non-septic shock model induced by zymosan in the rat.
AuthorsCuzzocrea S, Costantino G, Caputi AP
JournalShock
PubMed ID10030802
'Recently, it was proposed that zymosan, a nonbacterial agent, causes cellular injury by inducing the production of peroxynitrite and consequent poly-(ADP-ribose) synthetase (PARS activation). Here we investigated whether in vivo N-acetylcysteine treatment inhibits cellular injury in macrophages collected from rats subjected to zymosan-induced shock. Macrophages harvested from the peritoneal cavity ... More
Oxidative burst measurement in patients treated with cytostatics: influence of G-CSF and role as a prognostic factor.
AuthorsVolk J, Kleine HD, Buthmann U, Freund M
JournalAnn Hematol
PubMed ID10834506
'The ability to generate reactive oxygen species, the so-called oxidative burst, is essential for neutrophils to kill infectious micro-organisms. Flow cytometry was used to study oxidative burst prior to, during, and after cytostatic therapy. Seven patients were treated according to the DexaBEAM regimen with 12 cycles monitored. Four patients were ... More
Inactivation of mitochondrial electron transport by photosensitization of a pheophorbide a derivative.
AuthorsKim CS, Lee CH, Lee PH, Han S
JournalMol Cells
PubMed ID15179053
'We examined the damage to mitochondrial electron transport caused by photosensitization of a pheophorbide a derivative, DH-I-180-3, shown recently to induce necrosis of lung carcinoma cells with low dark toxicity. Confocal microscopy showed that DH-I-180-3 co-localized with dihydrorhodamine-123 suggesting that it mainly accumulates in mitochondria. The photosensitizer alone in the ... More
Age-related changes of the multidrug resistance P-glycoprotein function in normal human peripheral blood T lymphocytes.
AuthorsMachado CG, Calado RT, Garcia AB, Falcão RP
JournalBraz J Med Biol Res
PubMed ID14666249
'The multidrug resistance P-glycoprotein is a transmembrane efflux pump expressed by lymphocytes and is involved in their cytolytic activity. In the present study, we investigated the age-related changes of P-glycoprotein function in normal peripheral blood lymphocytes. Blood samples from 90 normal volunteers (age range, 0 to 86 years) were analyzed. ... More
Fluorescence probes used for detection of reactive oxygen species.
AuthorsGomes A, Fernandes E, Lima JL,
JournalJ Biochem Biophys Methods
PubMed ID16297980
'Endogenously produced pro-oxidant reactive species are essential to life, being involved in several biological functions. However, when overproduced (e.g. due to exogenous stimulation), or when the levels of antioxidants become severely depleted, these reactive species become highly harmful, causing oxidative stress through the oxidation of biomolecules, leading to cellular damage ... More
Signal transduction in monocytes and granulocytes measured by multiparameter flow cytometry.
AuthorsLund-Johansen F, Olweus J
JournalCytometry
PubMed ID1451600
'The novel calcium indicator fura red and the oxidative burst indicator dihydrorhodamine (both excited at 488 nm) were used in combination with multiparameter flow cytometry to allow simultaneous kinetic measurements of calcium fluxes and oxidative bursts in monocytes and granulocytes. Using this method it was possible to obtain direct evidence ... More
Outer membrane vesicles from Neisseria meningitidis.
AuthorsMirlashari MR, Høiby EA, Holst J, Lyberg T
JournalAPMIS
PubMed ID12076272
'Flow cytometry was used to study the expression of leukocyte adhesion molecules CD11a, CD11b, CD11c, CD14, and CD62L (L-selectin) and production of reactive oxygen species (ROS) in an ex vivo human whole-blood system stimulated with lipopolysaccharide-containing outer membrane vesicles (LPS-OMV) from N. meningitidis. Results demonstrated a dose-dependent increase in surface ... More
Mitochondrial production of reactive oxygen species in cortical neurons following exposure to N-methyl-D-aspartate.
'Increasing evidence suggests that glutamate neurotoxicity is partly mediated by reactive oxygen species, formed as a consequence of several processes, including arachidonic acid metabolism and nitric oxide production. Here we used an oxidation-sensitive indicator, dihydrorhodamine 123, in combination with confocal microscopy, to examine the hypothesis that electron transport by neuronal ... More
Influence of polysaccharides from Viscum album L. on human lymphocytes, monocytes and granulocytes in vitro.
AuthorsStein GM, Edlund U, Pfüller U, Büssing A, Schietzel M
JournalAnticancer Res
PubMed ID10628330
'BACKGROUND: An acidic arabinogalactan from European mistletoe (Viscum album L, VAL; 1.34 x 10(6) Dalton) was studied in detail because its immunological properties are poorly characterised. MATERIALS AND METHODS: Flow cytometric studies focussed on PS-activated proliferation of human lymphocytes measured via incorporation of bromo-deoxyuridine (BrdU), granulocyte phagocytosis via ingestion of ... More
Time-course of oxygen free radical production in acinar cells during acute pancreatitis induced by pancreatic duct obstruction.
AuthorsUruñuela A, Sevillano S, de la Mano AM, Manso MA, Orfao A, de Dios I
JournalBiochim Biophys Acta
PubMed ID12385780
'The time-course of oxygen free radicals (OFR) generation within acinar cells was studied at different stages of acute pancreatitis (AP) induced in rats by duct obstruction (PDO) for 48 h by flow cytometry, using dihydrorhodamine-123 (DHR) as fluorescent dye. Parallel measurements of the most common markers of oxidative stress such ... More
Quantitative multiwell myeloid differentiation assay using dichlorodihydrofluorescein diacetate (H2DCF-DA) or dihydrorhodamine 123 (H2R123).
AuthorsTrayner ID, Rayner AP, Freeman GE, Farzaneh F
JournalJ Immunol Methods
PubMed ID7594627
'It is well established that the fluorescent probes dichlorodihydrofluorescein diacetate (H2DCF-DA) and dihydrorhodamine 123 (H2R123) can be used to detect the respiratory burst response of mature myeloid cells. We describe a simple, fast and quantitative assay for myeloid differentiation based on the oxidation of these probes, which can be performed ... More
Free radical production and changes in superoxide dismutases associated with hypoxia/reoxygenation-induced apoptosis of embryonic rat forebrain neurons in culture.
AuthorsLièvre V, Becuwe P, Bianchi A, Koziel V, Franck P, Schroeder H, Nabet P, Dauça M, Daval JL
JournalFree Radic Biol Med
PubMed ID11118819
'Following hypoxia/reoxygenation (6h/96h), cultured neurons from the embryonic rat forebrain undergo delayed apoptosis. To evaluate the participation of oxidative stress and defense mechanisms, temporal evolution of intraneuronal free radical generation was monitored by flow cytometry using dihydrorhodamine 123, in parallel with the study of transcriptional, translational, and activity changes of ... More
Effect of melatonin on cellular energy depletion mediated by peroxynitrite and poly (ADP-ribose) synthetase activation in an acute model of inflammation.
AuthorsDugo L, Serraino I, Fulia F, De Sarro A, Caputi AP, Cuzzocrea S
JournalJ Pineal Res
PubMed ID11485008
'DNA single-strand breakage and activation of the nuclear enzyme poly (ADP-ribose) synthetase (PARS) triggers an energy-consuming, inefficient repair cycle, which contributes to peroxynitrite-induced cellular injury. Recently, we proposed that during an acute model (pleurisy), cellular injury is mediated by peroxynitrite formation and consequent PARS activation. Here, we investigated whether in ... More
Role of peroxynitrite and poly (ADP-ribosyl) synthetase activation in cardiovascular derangement induced by zymosan in the rat.
AuthorsCuzzocrea S, Zingarelli B, Caputi AP
JournalLife Sci
PubMed ID9747893
'Peritoneal administration of zymosan in the rat induced a severe inflammatory process characterised by an increase in the plasma levels of nitrite and nitrate, stable metabolites of nitric oxide (NO) and in the levels of peroxynitrite, as measured by the oxidation of the fluorescent dye dihydrorhodamine 123, at 18 hours ... More
P-glycoprotein and multidrug resistance-associated protein mediate specific patterns of multidrug resistance in malignant glioma cell lines, but not in primary glioma cells.
AuthorsBähr O, Rieger J, Duffner F, Meyermann R, Weller M, Wick W
JournalBrain Pathol
PubMed ID14655754
'Understanding and overcoming multidrug resistance (MDR) may be a promising strategy to develop more effective pharmacotherapies for malignant gliomas. In the present study, human malignant glioma cell lines (n=12) exhibited heterogeneous mRNA and protein expression and functional activity of the mdr gene-encoded P-glycoprotein (PGP) and MDR-associated protein (MRP). Correlation between ... More
Patatin, the tuber storage protein of potato (Solanum tuberosum L.), exhibits antioxidant activity in vitro.
AuthorsLiu YW, Han CH, Lee MH, Hsu FL, Hou WC
JournalJ Agric Food Chem
PubMed ID12848515
'The potato (Solanum tuberosum L.) tuber storage protein, patatin, was purified to homogeneity with a molecular mass of 45 kDa. The purified patatin showed antioxidant or antiradical activity by a series of in vitro tests, including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical (half-inhibition concentration, IC(50), was 0.582 mg/mL) scavenging activity assays, anti-human low-density ... More
Water-soluble organotellurium compounds: catalytic protection against peroxynitrite and release of zinc from metallothionein.
AuthorsJacob C, Arteel GE, Kanda T, Engman L, Sies H
JournalChem Res Toxicol
PubMed ID10649960
'The antioxidant properties of a number of water-soluble diorganyl tellurides have been investigated. These organotellurium compounds efficiently protect against peroxynitrite-mediated oxidation of dihydrorhodamine 123, hydroxylation of benzoate, and nitration of 4-hydroxyphenyl acetate. The peroxidation of the zinc storage protein, metallothionein, by tert-butyl hydroperoxide is also catalyzed by the water-soluble organotellurium ... More