JournalAdvances in experimental medicine and biology
PubMed ID8109389
The effect of Ca2+ on the time course of force generation in frog skinned muscle fibres has been investigated using laser flash photolysis of the caged-calcium, either nitr-5 or DM-Nitrophen. Gradations in the rate and extent of contraction could be achieved by changing the energy of the laser pulse, which ... More
Correlation between mechanical and enzymatic events in contracting skeletal muscle fiber.
AuthorsShepard A, Borejdo J
JournalBiochemistry
PubMed ID15005615
'The conventional hypothesis of muscle contraction postulates that the interaction between actin and myosin involves tight coupling between the power stroke and hydrolysis of ATP. However, some in vitro experiments suggested that hydrolysis of a single molecule of ATP caused multiple mechanical cycles. To test whether the tight coupling is ... More
Calcium transients in astrocyte endfeet cause cerebrovascular constrictions.
AuthorsMulligan SJ, MacVicar BA
JournalNature
PubMed ID15356633
Cerebral blood flow (CBF) is coupled to neuronal activity and is imaged in vivo to map brain activation. CBF is also modified by afferent projection fibres that release vasoactive neurotransmitters in the perivascular region, principally on the astrocyte endfeet that outline cerebral blood vessels. However, the role of astrocytes in ... More
No evidence for calcium electrogenic exchanger in frog semicircular canal hair cells.
AuthorsMartini M, Rossi ML, Farinelli F, Moriondo A, Mammano F, Rispoli G
JournalEur J Neurosci
PubMed ID12431216
We investigated the possibility that, in hair cells mechanically isolated from frog semicircular canals, Ca2+ extrusion occurs via a Na+ : Ca2+ (cardiac type) or a Na+ : Ca2+,K+ (retinal type) exchanger. Cells concurrently imaged during whole-cell patch-clamp recordings using the Ca2+ sensitive fluorescent dye Oregon Green 488 BAPTA-1 (100 ... More