'Using a novel method for retrogradely labeling specific neuronal populations, we tested different styryl dyes in attempt to find dyes whose staining would be specific, rapid, and lead to large activity dependent signals. The dyes were injected into the ventral roots of the isolated chick spinal cord from embryos at ... More
Intrasarcomere [Ca2+] gradients and their spatio-temporal relation to Ca2+ sparks in rat cardiomyocytes.
AuthorsTanaka H, Sekine T, Kawanishi T, Nakamura R, Shigenobu K
JournalJ Physiol
PubMed ID9490830
'1. Line-scan analyses of spontaneous Ca2+ sparks, non-propagating local rises in Ca2+ concentration, and the early phase of Ca2+ transients in cardiomyocytes were performed with a rapid-scanning laser confocal microscope (Nikon RCM8000) and fluo-3. 2. On electrical stimulation, points at which rise in Ca2+ began earliest were observed at regular ... More
Optical recording from cerebellar Purkinje cells using intracellularly injected voltage-sensitive dyes.
AuthorsKogan A, Ross WN, Zecevic D, Lasser-Ross N
JournalBrain Res
PubMed ID8624715
'We evaluated several techniques for their ability to record membrane potential changes with voltage-sensitive dyes introduced into CNS neurons in the brain slice preparation. Using a probe designed for intracellular application, JPW1114, we found that iontophoresis or pressure pulses could not push the lipophilic dye through electrodes whose resistance was ... More
Visual stimuli induce waves of electrical activity in turtle cortex.
AuthorsPrechtl JC, Cohen LB, Pesaran B, Mitra PP, Kleinfeld D
JournalProc Natl Acad Sci U S A
PubMed ID9207142
'The computations involved in the processing of a visual scene invariably involve the interactions among neurons throughout all of visual cortex. One hypothesis is that the timing of neuronal activity, as well as the amplitude of activity, provides a means to encode features of objects. The experimental data from studies ... More
Comparison of fluorescent voltage-sensitive dyes for multisite optical recording in hamster cerebral cortex by measurement of bicuculline-induced epileptiform events.
AuthorsdeBeer Zeiger J
JournalNeuroimage
PubMed ID9345545
'Two fluorescent voltage-sensitive dyes, RH795 and DI-2-ANEPPQ, were compared for in vivo multisite optical recording from the gustatory insular cortex of the golden Syrian hamster, the first reported use of DI-2-ANEPPQ in a mammalian brain preparation. The exposed cortex of the anesthetized hamster was stained with a 500 microM solution ... More
Synthesis, spectra, delivery and potentiometric responses of new styryl dyes with extended spectral ranges.
'Styryl dyes have been among the most widely used probes for mapping membrane potential changes in excitable cells. However, their utility has been somewhat limited because their excitation wavelengths have been restricted to the 450-550 nm range. Longer wavelength probes can minimize interference from endogenous chromophores and, because of decreased ... More
Voltage-sensitive dye imaging: Technique review and models.
AuthorsChemla S, Chavane F,
JournalJ Physiol Paris
PubMed ID19909809
In this review, we present the voltage-sensitive dye imaging (VSDI) method. The possibility offered for in vivo (and in vitro) brain imaging is unprecedented in terms of spatial and temporal resolution. However, the unresolved multi-component origin of the optical signal encourages us to perform a detailed analysis of the method ... More
Imaging membrane potential with voltage-sensitive dyes.
AuthorsZochowski M, Wachowiak M, Falk CX, Cohen LB, Lam YW, Antic S, Zecevic D
JournalBiol Bull
PubMed ID10707808
Membrane potential can be measured optically using a variety of molecular probes. These measurements can be useful in studying function at the level of an individual cell, for determining how groups of neurons generate a behavior, and for studying the correlated behavior of populations of neurons. Examples of the three ... More
Cholesterol-enriched lipid domains can be visualized by di-4-ANEPPDHQ with linear and nonlinear optics.
AuthorsJin L, Millard AC, Wuskell JP, Clark HA, Loew LM
JournalBiophys J
PubMed ID15879475
We present a membrane-staining dye, di-4-ANEPPDHQ, which differentiates liquid-ordered phases from liquid-disordered phases coexisting in model membranes under both linear and nonlinear microscopies. The dye's fluorescence emission spectrum is blue-shifted 60 nm in liquid-ordered phases compared with liquid-disordered phases, and shows strong second harmonic generation in the liquid-disordered phase compared ... More
Imaging inhibitory synaptic potentials using voltage sensitive dyes.
AuthorsCanepari M, Willadt S, Zecevic D, Vogt KE,
JournalBiophys J
PubMed ID20441768
Studies of the spatio-temporal distribution of inhibitory postsynaptic potentials (IPSPs) in a neuron have been limited by the spatial information that can be obtained by electrode recordings. We describe a method that overcomes these limitations by imaging IPSPs with voltage-sensitive dyes. CA1 hippocampal pyramidal neurons from brain slices were loaded ... More
Wide-field and two-photon imaging of brain activity with voltage- and calcium-sensitive dyes.
AuthorsHomma R, Baker BJ, Jin L, Garaschuk O, Konnerth A, Cohen LB, Bleau CX, Canepari M, Djurisic M, Zecevic D,
JournalMethods Mol Biol
PubMed ID18839087
This chapter presents three examples of imaging brain activity with voltage- or calcium-sensitive dyes. Because experimental measurements are limited by low sensitivity, the chapter then discusses the methodological aspects that are critical for optimal signal-to-noise ratio. Two of the examples use wide-field (1-photon) imaging and the third uses two-photon scanning ... More
Multiple spike-initiation zones in single neurons revealed by voltage-sensitive dyes.
AuthorsZecevic D
JournalNature
PubMed ID8692270
The primary function of the nerve cell is to process electrical signals. Over the past fifteen years there has been renewed interest in the detailed spatial analysis of signalling in individual neurons owing to experimental evidence that the regional electrical properties of neurons are complex. Thus the behaviour of many ... More
Functional profile of the giant metacerebral neuron of Helix aspersa: temporal and spatial dynamics of electrical activity in situ.
AuthorsAntic S, Wuskell JP, Loew L, Zecevic D
JournalJ Physiol
PubMed ID10944170
1. Understanding the biophysical properties of single neurons and how they process information is fundamental to understanding how the brain works. However, action potential initiation and the preceding integration of the synaptic signals in neuronal processes of individual cells are complex and difficult to understand in the absence of detailed, ... More
Initiation of sodium spikelets in basal dendrites of neocortical pyramidal neurons.
Cortical information processing relies critically on the processing of electrical signals in pyramidal neurons. Electrical transients mainly arise when excitatory synaptic inputs impinge upon distal dendritic regions. To study the dendritic aspect of synaptic integration one must record electrical signals in distal dendrites. Since thin dendritic branches, such as oblique ... More
Indicators and optical configuration for simultaneous high-resolution recording of membrane potential and intracellular calcium using laser scanning microscopy.
AuthorsBullen A, Saggau P
JournalPflugers Arch
PubMed ID9716714
The instrumental design and experimental conditions for high-speed, simultaneous optical recording of membrane potential and intracellular Ca2+ with subcellular resolution are presented. This method employs an extended version of a high-speed, random-access, laser-scanning fluorescence microscope designed to record fast physiological signals from small neuronal structures with high spatiotemporal resolution (Bullen, ... More
Optical signals from neurons with internally applied voltage-sensitive dyes.
AuthorsAntic S, Zecevic D
JournalJ Neurosci
PubMed ID7869106
We carried out experiments to monitor optically the generation and spread of action potentials and subthreshold potentials in the processes of individual neurons in ganglia of the snail, Helix aspersa. The neurons were selectively stained by intracellular pressure injection of voltage-sensitive dyes. Optical signals were detected by a system for ... More
The spatio-temporal characteristics of action potential initiation in layer 5 pyramidal neurons: a voltage imaging study.
Authors
JournalJ Physiol
PubMed ID21669974
Wide-field and two-photon imaging of brain activity with voltage- and calcium-sensitive dyes.
Authors
JournalPhilos Trans R Soc Lond B Biol Sci
PubMed ID19651647
Saltatory Conduction along Myelinated Axons Involves a Periaxonal Nanocircuit.
Authors
JournalCell
PubMed ID31883793
Loss of Saltation and Presynaptic Action Potential Failure in Demyelinated Axons.
Authors
JournalFront Cell Neurosci
PubMed ID28289377
Imaging membrane potential changes from dendritic spines using computer-generated holography.
Authors
JournalNeurophotonics
PubMed ID28523281
Functional structure of the mitral cell dendritic tuft in the rat olfactory bulb.
Authors
JournalJ Neurosci
PubMed ID18400905
Electrophysiological events recorded at presynaptic terminals of the crayfish neuromuscular junction with a voltage indicator.
Authors
JournalJ Physiol
PubMed ID18755747
Electrical behaviour of dendritic spines as revealed by voltage imaging.
Authors
JournalNat Commun
PubMed ID26436431
Dendritic signals from rat hippocampal CA1 pyramidal neurons during coincident pre- and post-synaptic activity: a combined voltage- and calcium-imaging study.
Authors
JournalJ Physiol
PubMed ID17272348
Cortical dendritic spine heads are not electrically isolated by the spine neck from membrane potential signals in parent dendrites.