'Using a novel method for retrogradely labeling specific neuronal populations, we tested different styryl dyes in attempt to find dyes whose staining would be specific, rapid, and lead to large activity dependent signals. The dyes were injected into the ventral roots of the isolated chick spinal cord from embryos at ... More
Cholesterol-enriched lipid domains can be visualized by di-4-ANEPPDHQ with linear and nonlinear optics.
AuthorsJin L, Millard AC, Wuskell JP, Clark HA, Loew LM
JournalBiophys J
PubMed ID15879475
We present a membrane-staining dye, di-4-ANEPPDHQ, which differentiates liquid-ordered phases from liquid-disordered phases coexisting in model membranes under both linear and nonlinear microscopies. The dye's fluorescence emission spectrum is blue-shifted 60 nm in liquid-ordered phases compared with liquid-disordered phases, and shows strong second harmonic generation in the liquid-disordered phase compared ... More
Voltage-sensitive dye recording using retrogradely transported dye in the chicken spinal cord: staining and signal characteristics.
AuthorsWenner P, Tsau Y, Cohen LB, O'Donovan MJ, Dan Y
JournalJ Neurosci Methods
PubMed ID9007750
We describe a novel method for retrogradely labeling specific neuronal populations using voltage-sensitive dyes. Styryl dyes were injected into the ventral roots of the isolated embryonic chick spinal cord. After waiting several hours, the dye labeled motoneurons and autonomic preganglionic neurons. Neuronal cell bodies, dendrites and axons were labeled; we ... More
Chemotopic, combinatorial, and noncombinatorial odorant representations in the olfactory bulb revealed using a voltage-sensitive axon tracer.
AuthorsFriedrich RW, Korsching SI
JournalJ Neurosci
PubMed ID9822753
Odor information is first represented in the brain by patterns of input activity across the glomeruli of the olfactory bulb (OB). To examine how odorants are represented at this stage of olfactory processing, we labeled anterogradely the axons of olfactory receptor neurons with the voltage-sensitive dye Di8-ANEPPQ in zebrafish. The ... More
An immediate endothelial cell signaling response to lung ischemia.
AuthorsSong C, Al-Mehdi AB, Fisher AB
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID11557603
Abrupt cessation of lung perfusion induces a rapid endothelial response that is not associated with anoxia but reflects loss of normal shear stress. This response includes membrane depolarization, H(2)O(2) generation, and increased intracellular Ca(2+). We evaluated these parameters immediately upon nonhypoxic ischemia using fluorescence videomicroscopy to image in situ endothelial ... More