T-cell priming by dendritic cells in lymph nodes occurs in three distinct phases.
AuthorsMempel TR, Henrickson SE, Von Andrian UH
JournalNature
PubMed ID14712275
'Primary T-cell responses in lymph nodes (LNs) require contact-dependent information exchange between T cells and dendritic cells (DCs). Because lymphocytes continually enter and leave normal LNs, the resident lymphocyte pool is composed of non-synchronized cells with different dwell times that display heterogeneous behaviour in mouse LNs in vitro. Here we ... More
Transport in lymphatic capillaries. II. Microscopic velocity measurement with fluorescence photobleaching.
AuthorsBerk DA, Swartz MA, Leu AJ, Jain RK
JournalAm J Physiol
PubMed ID8769769
'Despite its relevance to the physiology of lymph formation and propulsion, the instantaneous flow velocity in single lymphatic capillaries has not been measured to date. The method of fluorescence recovery after photobleaching (FRAP) was adapted for this purpose and used to characterize flow in the lymphatic capillaries in tail skin ... More
Optimization of a microfluidic mixer for studying protein folding kinetics.
AuthorsHertzog DE, Ivorra B, Mohammadi B, Bakajin O, Santiago JG
JournalAnal Chem
PubMed ID16808436
'We have applied an optimization method in conjunction with numerical simulations to minimize the mixing time of a microfluidic mixer developed for protein folding studies. The optimization method uses a semideterministic algorithm to find the global minimum of the mixing time by varying the mixer geometry and flow conditions. We ... More
Contribution of early cells to the fate map of the zebrafish gastrula.
AuthorsHelde KA, Wilson ET, Cretekos CJ, Grunwald DJ
JournalScience
PubMed ID8036493
Previously, a tissue-specific fate map was compiled for the gastrula stage of the zebrafish embryo, indicating that development subsequent to this stage follows a reproducible pattern. Here it is shown that each early zebrafish blastomere normally contributes to a subset of the gastrula and thus gives rise to a limited ... More
Structure and composition of aggregates in two large European rivers, based on confocal laser scanning microscopy and image and statistical analyses.
AuthorsLuef B, Neu TR, Zweimüller I, Peduzzi P,
JournalAppl Environ Microbiol
PubMed ID19633114
Floating riverine aggregates are composed of a complex mixture of inorganic and organic components from their respective aquatic habitats. Their architecture and integrity are supplemented by the presence of extracellular polymeric substances of microbial origin. They are also a habitat for virus-like particles, bacteria, archaea, fungi, algae, and protozoa. In ... More
Restricted dextran transport in the sheep lung lymph preparation.
AuthorsMcNamee JE
JournalJ Appl Physiol
PubMed ID6175612
The ease with which plasma proteins and other large solutes enter sheep lung lymph had led previous investigators to conclude that such large molecules probably move through a pathway that has transport properties similar to pores 100 nm or larger in radius. To test this hypothesis, the transport of some ... More
Annexin A2 supports pulmonary microvascular integrity by linking vascular endothelial cadherin and protein tyrosine phosphatases.
Authors
JournalJ Exp Med
PubMed ID28694388
Type I Interferons Suppress Anti-parasitic Immunity and Can Be Targeted to Improve Treatment of Visceral Leishmaniasis.
Authors
JournalCell Rep
PubMed ID32101732
Targeting the SHP2 phosphatase promotes vascular damage and inhibition of tumor growth.
Authors
JournalEMBO Mol Med
PubMed ID34102002
Quaking orchestrates a post-transcriptional regulatory network of endothelial cell cycle progression critical to angiogenesis and metastasis.
Authors
JournalOncogene
PubMed ID30918328
IL-22 promotes the formation of a MUC17 glycocalyx barrier in the postnatal small intestine during weaning.
Authors
JournalCell Rep
PubMed ID33596425
IL-27 signalling regulates glycolysis in Th1 cells to limit immunopathology during infection.
Authors
JournalPLoS Pathog
PubMed ID33049000
Bid, Bax, and lipids cooperate to form supramolecular openings in the outer mitochondrial membrane.
Authors
JournalCell
PubMed ID12419244
In vivo cell biological screening identifies an endocytic capture mechanism for T-tubule formation.
Authors
JournalNat Commun
PubMed ID32709891
Cancer Lipid Metabolism Confers Antiangiogenic Drug Resistance.
Authors
JournalCell Metab
PubMed ID29861385
Therapeutic paradigm of dual targeting VEGF and PDGF for effectively treating FGF-2 off-target tumors.