T-cell priming by dendritic cells in lymph nodes occurs in three distinct phases.
AuthorsMempel TR, Henrickson SE, Von Andrian UH
JournalNature
PubMed ID14712275
'Primary T-cell responses in lymph nodes (LNs) require contact-dependent information exchange between T cells and dendritic cells (DCs). Because lymphocytes continually enter and leave normal LNs, the resident lymphocyte pool is composed of non-synchronized cells with different dwell times that display heterogeneous behaviour in mouse LNs in vitro. Here we ... More
Controlled mixing in microfluidic systems using bacterial chemotaxis.
AuthorsKim MJ, Breuer KS
JournalAnal Chem
PubMed ID17263321
We demonstrate the use of Escherichia coli and their chemotactic characteristics to enhance mixing in a microchannel in a controlled and bi-directional manner. The presence of a chemoattractant in one arm of a three-junction microchannel results in an asymmetric increase in the effective diffusion coefficient of extremely high molecular weight ... More
Structure and composition of aggregates in two large European rivers, based on confocal laser scanning microscopy and image and statistical analyses.
AuthorsLuef B, Neu TR, Zweimüller I, Peduzzi P,
JournalAppl Environ Microbiol
PubMed ID19633114
Floating riverine aggregates are composed of a complex mixture of inorganic and organic components from their respective aquatic habitats. Their architecture and integrity are supplemented by the presence of extracellular polymeric substances of microbial origin. They are also a habitat for virus-like particles, bacteria, archaea, fungi, algae, and protozoa. In ... More
Selective release of molecules from Weibel-Palade bodies during a lingering kiss.
AuthorsBabich V, Meli A, Knipe L, Dempster JE, Skehel P, Hannah MJ, Carter T,
JournalBlood
PubMed ID18252862
Exocytosis of specialized endothelial cell secretory organelles, Weibel-Palade bodies (WPBs), is thought to play an important role in regulating hemostasis and intravascular inflammation. The major WPB core proteins are Von Willebrand factor (VWF) and its propolypeptide (Proregion), constituting more than 95% of the content. Although the composition of the WPBs ... More
FACS-based isolation of slowly growing cells: double encapsulation of yeast in gel microdrops.
AuthorsGift EA, Park HJ, Paradis GA, Demain AL, Weaver JC
JournalNat Biotechnol
PubMed ID9631016
Isolating hyperproducing cells is important in biotechnology, but these cells usually grow slowly and can be overgrown by poorly producing cells. We describe a new method of isolating slowly growing cells from among rapidly growing cells, which has the potential for automation and high throughput (e.g., 100,000 cells/h). A model ... More
Angiogenesis monitored by perfusion with a space-filling microbead suspension.
AuthorsSpringer ML, Ip TK, Blau HM
JournalMol Ther
PubMed ID10933915
Numerous laboratories are focusing efforts on delivering gene products to induce or prevent the development of new blood vessels in adults, with the hope of rescuing ischemic tissues, circumventing cardiac bypass surgery, or inhibiting tumor growth. Current approaches to the assessment of vascular continuity involve the introduction of either dyes ... More
Activities of the Wnt-1 class of secreted signaling factors are antagonized by the Wnt-5A class and by a dominant negative cadherin in early Xenopus development.
When overexpressed in Xenopus embryos, Xwnt-1, -3A, -8 and -8b define a functional class of Wnts (the Wnt-1 class) that promotes duplication of the embryonic axis, whereas Xwnt-5A, -4, and -11 define a distinct class (the Wnt-5A class) that alters morphogenetic movements (Du, S., S. Purcell, J. Christian, L. McGrew, ... More
Phase separation directs ubiquitination of gene-body nucleosomes.
Fabrication and operation of GRIN probes for in vivo fluorescence cellular imaging of internal organs in small animals.
Authors
JournalNat Protoc
PubMed ID22767088
Endothelial Notch activity promotes angiogenesis and osteogenesis in bone.
Authors
JournalNature
PubMed ID24647000
Acute cellular and vascular responses to photodynamic therapy using EGFR-targeted nanobody-photosensitizer conjugates studied with intravital optical imaging and magnetic resonance imaging.
Authors
JournalTheranostics
PubMed ID32089747
Imaging the construction of capillary networks in the neonatal mouse brain.
Authors
JournalProc Natl Acad Sci U S A
PubMed ID34172585
Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network.
Authors
JournalCell Stem Cell
PubMed ID31303547
Discontinuation of anti-VEGF cancer therapy promotes metastasis through a liver revascularization mechanism.
Authors
JournalNat Commun
PubMed ID27580750
Cancer Lipid Metabolism Confers Antiangiogenic Drug Resistance.
Authors
JournalCell Metab
PubMed ID29861385
Therapeutic paradigm of dual targeting VEGF and PDGF for effectively treating FGF-2 off-target tumors.