'None of the anterograde tracers used to label and investigate vagal preganglionic neurons projecting to the viscera has proved optimal for routine and extensive labeling of autonomic terminal fields. To identify an alternative tracer protocol, the present experiment evaluated whether dextran conjugates, which have produced superior results in the CNS, ... More
Activation of lysosomal function during dendritic cell maturation.
AuthorsTrombetta ES, Ebersold M, Garrett W, Pypaert M, Mellman I
JournalScience
PubMed ID12610307
In response to a variety of stimuli, dendritic cells (DCs) transform from immature cells specialized for antigen capture into mature cells specialized for T cell stimulation. During maturation, the DCs acquire an enhanced capacity to form and accumulate peptide-MHC (major histocompatibility complex) class II complexes. Here we show that a ... More
A novel method for simultaneous anterograde and retrograde labeling of spinal cord motor tracts in the same animal.
AuthorsTsai EC, van Bendegem RL, Hwang SW, Tator CH
JournalJ Histochem Cytochem
PubMed ID11511680
Examination of repaired spinal cord tracts has usually required separate groups of animals for anterograde and retrograde tracing owing to the incompatibility of techniques such as tissue fixation. However, anterograde and retrograde labeling of different animals subjected to the same repair may not allow accurate examination of that repair strategy ... More