'The intracellular activation of trypsinogen, which is both pH- and calcium-dependent, is an important early step in the development of acute pancreatitis. The cellular compartment in which trypsinogen activation occurs currently is unknown. We therefore investigated the site of intracellular trypsinogen activation by using an established cellular model of acute ... More
Reconstitution of membrane proteins into giant unilamellar vesicles via peptide-induced fusion.
AuthorsKahya N, Pécheur EI, de Boeij WP, Wiersma DA, Hoekstra D
JournalBiophys J
PubMed ID11509360
In this work, we present a protocol to reconstitute membrane proteins into giant unilamellar vesicles (GUV) via peptide-induced fusion. In principle, GUV provide a well-defined lipid matrix, resembling a close-to-native state for biophysical studies, including optical microspectroscopy, of transmembrane proteins at the molecular level. Furthermore, reconstitution in this manner would ... More