Traffic pathways of Plasmodium vivax antigens during intraerythrocytic parasite development.
AuthorsBracho C, Dunia I, De LR, Benedetti EL, Perez HA
JournalParasitol Res
PubMed ID11954911
'We investigated the secretory traffic of a Plasmodium vivax antigen (Pv-148) synthesised by the parasite during the blood cycle, exported into the host cell cytosol and then transported to the surface membrane of the infected erythrocyte. Studies of the ultrastructure of erythrocytes infected with P. vivax showed that intracellular schizogony ... More
Observation of polarity induction by cytochemical localization of phenylalkylamine-binding sites in regenerating protoplasts of the moss Physcomitrella patens.
AuthorsBhatla SC, Kiessling J, Reski R
JournalProtoplasma
PubMed ID11926072
'Different external (e.g., light) and internal (e.g., auxin and calcium gradients) factors control differentiation of the moss protonema. The present investigations demonstrate that exogenously applied auxin, the pharmacological blockade of auxin efflux by naphthylphthalamic acid, and treatment with (-)bepridil, a calcium channel antagonist, inhibit protoplast division without affecting protoplast viability ... More
Studies on ion channel antagonist-binding sites in sunflower protoplasts.
AuthorsVallée N, Brière C, Petitprez M, Barthou H, Souvré A, Alibert G
JournalFEBS Lett
PubMed ID9247154
The cytological location of ion channel antagonist-binding sites was studied in sunflower protoplasts using the fluorescent probes DM-Bodipy-PAA and DM-Bodipy-DHP. The binding specificity of the probes was established by competition experiments with Bepridil, phenylalkylamine (Verapamil) and dihydropyridine (Nifedipine) which are known as calcium and potassium channel antagonists. Quantitative image analysis ... More
HIV-1 Tat inhibits human natural killer cell function by blocking L-type calcium channels.
AuthorsZocchi MR, Rubartelli A, Morgavi P, Poggi A
JournalJ Immunol
PubMed ID9743356
Herein we show that functional phenylalkylamine-sensitive L-type calcium channels are expressed by human NK cells and are involved in the killing of tumor targets. Blocking of these channels by phenylalkylamine drugs does not affect effector/target cell binding but inhibits the release of serine esterases responsible for cytotoxicity. Interestingly, treatment of ... More
Localization of L-type Ca2+ channels at perisynaptic glial cells of the frog neuromuscular junction.
AuthorsRobitaille R, Bourque MJ, Vandaele S
JournalJ Neurosci
PubMed ID8613781
The presence of L-type Ca2+ channels at the frog neuromuscular junction (nmj) was studied by monitoring changes in intracellular Ca2+ evoked in presynaptic terminals and perisynaptic Schwann cells (PSCs) and by studying the distribution of Ca2+ channels using a monoclonal antibody directed against the alpha 2/delta subunit of L channels. ... More
A light stabilizer (Tinuvin 770) that elutes from polypropylene plastic tubes is a potent L-type Ca(2+)-channel blocker.
AuthorsGlossmann H, Hering S, Savchenko A, Berger W, Friedrich K, Garcia ML, Goetz MA, Liesch JM, Zink DL, Kaczorowski GJ
JournalProc Natl Acad Sci U S A
PubMed ID8415734
A pharmacologically active agent was easily extracted by aqueous or organic solvents from laboratory plastic tubes (Falcon Blue Max) and has been chemically identified as bis(2,2,6,6-tetramethyl-4-piperidyl) sebacate. This compound (approximately 12 micrograms per tube approximately 25 nmol) blocked 1,4-dihydropyridine-sensitive 45Ca2+ uptake into GH3 cells with an IC50 value of 3.6 ... More
A unique fluorescent phenylalkylamine probe for L-type Ca2+ channels. Coupling of phenylalkylamine receptors to Ca2+ and dihydropyridine binding sites.
AuthorsKnaus HG, Moshammer T, Kang HC, Haugland RP, Glossmann H
JournalJ Biol Chem
PubMed ID1310311
The first fluorescently labeled phenylalkylamine, DMBODIPY-PAA (5-(3-[3-(4,4-difluoro-5,7-dimethyl-3a, 4a-diaza-4-bora-indacen-3-yl)propionamido] phenethyl-N-methylamino)-2-isopropyl-2-(3,4,5-trimethoxyphenyl)-valer onitrile) has been introduced for L-type Ca2+ channel research. DMBODIPY-PAA binds reversibly to L-type Ca2+ channels purified from rabbit skeletal muscle microsomes by wheat germ agglutinin-Sepharose chromatography. In this preparation DMBODIPY-PAA labels 412 pmol of phenylalkylamine receptors/mg of protein with a ... More