Role of the CDC25 homology domain of phospholipase Cepsilon in amplification of Rap1-dependent signaling.
AuthorsJin TG, Satoh T, Liao Y, Song C, Gao X, Kariya K, Hu CD, Kataoka T
JournalJ Biol Chem
PubMed ID11395506
Phospholipase Cepsilon (PLCepsilon) is a novel class of phosphoinositide-specific PLC characterized by possession of CDC25 homology and Ras/Rap1-associating domains. We and others have shown that human PLCepsilon is translocated from the cytoplasm to the plasma membrane and activated by direct association with Ras at its Ras/Rap1-associating domain. In addition, translocation ... More
The caveolae-mediated sv40 entry pathway bypasses the golgi complex en route to the endoplasmic reticulum.
AuthorsNorkin LC, Kuksin D
JournalVirol J
PubMed ID15840166
'BACKGROUND: Simian virus 40 (SV40) enters cells via an atypical caveolae-mediated endocytic pathway, which delivers the virus to a new intermediary compartment, the caveosome. The virus then is believed to go directly from the caveosome to the endoplasmic reticulum. Cholera toxin likewise enters via caveolae and traffics to caveosomes. But, ... More
Two-photon fluorescence absorption and emission spectra of dyes relevant for cell imaging.
AuthorsBestvater F, Spiess E, Stobrawa G, Hacker M, Feurer T, Porwol T, Berchner-Pfannschmidt U, Wotzlaw C, Acker H
JournalJ Microsc
PubMed ID12423261
'Two-photon absorption and emission spectra for fluorophores relevant in cell imaging were measured using a 45 fs Ti:sapphire laser, a continuously tuneable optical parametric amplifier for the excitation range 580-1150 nm and an optical multichannel analyser. The measurements included DNA stains, fluorescent dyes coupled to antibodies as well as organelle ... More
Cholesterol distribution in the Golgi complex of DITNC1 astrocytes is differentially altered by fresh and aged amyloid beta-peptide-(1-42).
AuthorsIgbavboa U, Pidcock JM, Johnson LN, Malo TM, Studniski AE, Yu S, Sun GY, Wood WG
JournalJ Biol Chem
PubMed ID12584199
'The Golgi complex plays an important role in cholesterol trafficking in cells, and amyloid beta-peptides (Abetas) alter cholesterol trafficking. The hypothesis was tested that fresh and aged Abeta-(1-42) would differentially modify Golgi cholesterol content in DINTC1 astrocytes and that the effects of Abeta-(1-42) would be associated with the region of ... More
Association between the 15-kDa selenoprotein and UDP-glucose:glycoprotein glucosyltransferase in the endoplasmic reticulum of mammalian cells.
AuthorsKorotkov KV, Kumaraswamy E, Zhou Y, Hatfield DL, Gladyshev VN
JournalJ Biol Chem
PubMed ID11278576
'Mammalian selenocysteine-containing proteins characterized with respect to function are involved in redox processes and exhibit distinct expression patterns and cellular locations. A recently identified 15-kDa selenoprotein (Sep15) has no homology to previously characterized proteins, and its function is not known. Here we report the intracellular localization and identification of a ... More
A stepwise dissection of the intracellular fate of cationic cell-penetrating peptides.
AuthorsFischer R, Köhler K, Fotin-Mleczek M, Brock R
JournalJ Biol Chem
PubMed ID14707144
'The role of endosomal acidification and retrograde transport for the uptake of the highly basic cell-penetrating peptides penetratin, Tat, and oligoarginine was investigated. The effect of a panel of drugs that interfere with discrete steps of endocytosis or Golgi-mediated transport on uptake and cellular distribution of fluorescein-labeled peptide analogues was ... More
GM130 and GRASP65-dependent lateral cisternal fusion allows uniform Golgi-enzyme distribution.
AuthorsPuthenveedu MA, Bachert C, Puri S, Lanni F, Linstedt AD
JournalNat Cell Biol
PubMed ID16489344
'The mammalian Golgi apparatus exists as stacks of cisternae that are laterally linked to form a continuous membrane ribbon, but neither the molecular requirements for, nor the purpose of, Golgi ribbon formation are known. Here, we demonstrate that ribbon formation is mediated by specific membrane-fusion events that occur during Golgi ... More
Acylation-dependent protein export in Leishmania.
AuthorsDenny PW, Gokool S, Russell DG, Field MC, Smith DF
JournalJ Biol Chem
PubMed ID10753904
'The surface of the protozoan parasite Leishmania is unusual in that it consists predominantly of glycosylphosphatidylinositol-anchored glycoconjugates and proteins. Additionally, a family of hydrophilic acylated surface proteins (HASPs) has been localized to the extracellular face of the plasma membrane in infective parasite stages. These surface polypeptides lack a recognizable endoplasmic ... More
Dynamics of the apical plasma membrane recycling system during cell division.
AuthorsHobdy-Henderson KC, Hales CM, Lapierre LA, Cheney RE, Goldenring JR
JournalTraffic
PubMed ID12956871
'The members of the family of Rab11 small GTPases are critical regulators of the plasma membrane vesicle recycling system. While previous studies have determined that the Golgi apparatus disperses during mitosis and reorganizes after cytokinesis, the fate of the recycling system during the cell cycle is more obscure. We have ... More
Competitive interactions of cancer cells and normal cells via secretory microRNAs.
AuthorsKosaka N, Iguchi H, Yoshioka Y, Hagiwara K, Takeshita F, Ochiya T,
JournalJ Biol Chem
PubMed ID22123823
'Normal epithelial cells regulate the secretion of autocrine and paracrine factors that prevent aberrant growth of neighboring cells, leading to healthy development and normal metabolism. One reason for tumor initiation is considered to be a failure of this homeostatic cell competitive system. Here we identify tumor-suppressive microRNAs (miRNAs) secreted by ... More
Non-covalent delivery of proteins into mammalian cells.
AuthorsLoudet A, Han J, Barhoumi R, Pellois JP, Burghardt RC, Burgess K,
JournalOrg Biomol Chem
PubMed ID19039359
Substances that mediate the import of proteins into cells,
The von Hippel-Lindau tumor suppressor targets to mitochondria.
AuthorsShiao YH, Resau JH, Nagashima K, Anderson LM, Ramakrishna G
JournalCancer Res
PubMed ID10850420
Subcellular localization of von Hippel-Lindau (VHL) tumor suppressor may clarify its role in tumorigenesis. In rat kidney, we observed a granular cytoplasmic immunostaining of VHL, as seen in human tissues. The green fluorescent protein (GFP)-tagged VHL also appeared as cytoplasmic granules in vitro and was colocalized with a mitochondrion-selective dye. ... More
Controlling the intracellular localization of fluorescent polyamide analogues in cultured cells.
AuthorsCrowley KS, Phillion DP, Woodard SS, Schweitzer BA, Singh M, Shabany H, Burnette B, Hippenmeyer P, Heitmeier M, Bashkin JK
JournalBioorg Med Chem Lett
PubMed ID12699756
The intracellular distribution of fluorescent-labeled polyamides was examined in live cells. We showed that BODIPY-labeled polyamides accumulate in acidic vesicles, mainly lysosomes, in the cytoplasm of HCT116 colon cancer cells and human rheumatoid synovial fibroblasts (RSF). Verapamil blocked vesicular accumulation and led to nuclear accumulation of the BODIPY-labeled polyamide in ... More
Rhes, a striatal-selective protein implicated in Huntington disease, binds beclin-1 and activates autophagy.
AuthorsMealer RG, Murray AJ, Shahani N, Subramaniam S, Snyder SH,
Journal
PubMed ID24324270
The protein mutated in Huntington disease (HD), mutant huntingtin (mHtt), is expressed throughout the brain and body. However, the pathology of HD is characterized by early and dramatic destruction selectively of the striatum. We previously reported that the striatal-specific protein Rhes binds mHtt and enhances its cytotoxicity. Moreover, Rhes-deleted mice ... More
Emilin3 is required for notochord sheath integrity and interacts with Scube2 to regulate notochord-derived Hedgehog signals.
AuthorsCorallo D, Schiavinato A, Trapani V, Moro E, Argenton F, Bonaldo P,
Journal
PubMed ID24131633
The notochord is a transient and essential structure that provides both mechanical and signaling cues to the developing vertebrate embryo. In teleosts, the notochord is composed of a core of large vacuolated cells and an outer layer of cells that secrete the notochord sheath. In this work, we have identified ... More
Leishmania requires Rab7-mediated degradation of endocytosed hemoglobin for their growth.
AuthorsPatel N, Singh SB, Basu SK, Mukhopadhyay A,
JournalProc Natl Acad Sci U S A
PubMed ID18319337
Leishmania is unable to synthesize heme and must acquire it from exogenous source, the mechanism of which is not known. We have shown that Leishmania endocytoses hemoglobin (Hb) and subsequently degrade it probably to generate heme. To understand how internalized Hb is degraded, we have cloned and expressed Rab7 homolog ... More
Copper-free click chemistry for dynamic in vivo imaging.
AuthorsBaskin JM, Prescher JA, Laughlin ST, Agard NJ, Chang PV, Miller IA, Lo A, Codelli JA, Bertozzi CR,
JournalProc Natl Acad Sci U S A
PubMed ID17942682
Dynamic imaging of proteins in live cells is routinely performed by using genetically encoded reporters, an approach that cannot be extended to other classes of biomolecules such as glycans and lipids. Here, we report a Cu-free variant of click chemistry that can label these biomolecules rapidly and selectively in living ... More
Glycolipid trafficking in Drosophila undergoes pathway switching in response to aberrant cholesterol levels.
AuthorsHortsch R, Lee E, Erathodiyil N, Hebbar S, Steinert S, Lee JY, Chua DS, Kraut R,
JournalMol Biol Cell
PubMed ID20053687
In lipid storage diseases, the intracellular trafficking of sphingolipids is altered by conditions of aberrant cholesterol accumulation. Drosophila has been used recently to model lipid storage diseases, but the effects of sterol accumulation on sphingolipid trafficking are not known in the fly, and the trafficking of sphingolipids in general has ... More
Actin assembly at membranes controlled by ARF6.
AuthorsSchafer DA, D'Souza-Schorey C, Cooper JA
JournalTraffic
PubMed ID11273133
The small GTPase, ADP-ribosylation factor-6 (ARF6), has been implicated in regulating membrane traffic and remodeling cortical F-actin. Using real-time video analysis of actin assembly in living cells, we investigated the function and mechanism of ARF6 in control of actin assembly. Expression of an activated form of ARF6 that mimicks the ... More
Role of dynactin in endocytic traffic: effects of dynamitin overexpression and colocalization with CLIP-170.
AuthorsValetti C, Wetzel DM, Schrader M, Hasbani MJ, Gill SR, Kreis TE, Schroer TA
JournalMol Biol Cell
PubMed ID10588646
The flow of material from peripheral, early endosomes to late endosomes requires microtubules and is thought to be facilitated by the minus end-directed motor cytoplasmic dynein and its activator dynactin. The microtubule-binding protein CLIP-170 may also play a role by providing an early link to endosomes. Here, we show that ... More
Insect cells encode a class II alpha-mannosidase with unique properties.
AuthorsKawar Z, Karaveg K, Moremen KW, Jarvis DL
JournalJ Biol Chem
PubMed ID11279010
Previously, we cloned and characterized an insect (Sf9) cell cDNA encoding a class II alpha-mannosidase with amino acid sequence and biochemical similarities to mammalian Golgi alpha-mannosidase II. Since then, it has been demonstrated that other mammalian class II alpha-mannosidases can participate in N-glycan processing. Thus, the present study was performed ... More
A proline-rich region and nearby cysteine residues target XLalphas to the Golgi complex region.
AuthorsUgur O, Jones TL
JournalMol Biol Cell
PubMed ID10749939
XLalphas is a splice variant of the heterotrimeric G protein, Galpha(s), found on Golgi membranes in cells with regulated and constitutive secretion. We examined the role of the alternatively spliced amino terminus of XLalphas for Golgi targeting with the use of subcellular fractionation and fluorescence microscopy. XLalphas incorporated [(3)H]palmitate, and ... More
A fluorescent probe of polyamine transport accumulates into intracellular acidic vesicles via a two-step mechanism.
AuthorsSoulet D, Gagnon B, Rivest S, Audette M, Poulin R
JournalJ Biol Chem
PubMed ID15208319
Mammalian polyamine carriers have not yet been molecularly identified. The fluoroprobe Spd-C2-BODIPY faithfully reports polyamine transport and accumulates almost exclusively in polyamine-sequestering vesicles (PSVs). Polyamines might thus be imported first by a plasma membrane carrier and then sequestered into pre-existing PSVs (model A), or be directly captured by polyamine receptors ... More
Infection of human endothelial cells with Bartonella bacilliformis is dependent on Rho and results in activation of Rho.
AuthorsVerma A, Davis GE, Ihler GM
JournalInfect Immun
PubMed ID10992508
Bartonella bacilliformis was continuously internalized into human endothelial cells beginning shortly after addition of the bacteria and continuing for at least 24 h after infection in vitro, with a major increase in uptake occurring between 16 and 24 h. Preincubation of endothelial cells with C3 exoenzyme, which inactivated intracellular Rho-GTPase, ... More
The missing link in coronavirus assembly. Retention of the avian coronavirus infectious bronchitis virus envelope protein in the pre-Golgi compartments and physical interaction between the envelope and membrane proteins.
AuthorsLim KP, Liu DX
JournalJ Biol Chem
PubMed ID11278557
One missing link in the coronavirus assembly is the physical interaction between two crucial structural proteins, the membrane (M) and envelope (E) proteins. In this study, we demonstrate that the coronavirus infectious bronchitis virus E can physically interact, via a putative peripheral domain, with M. Deletion of this domain resulted ... More
Changes in protein kinase C epsilon phosphorylation status and intracellular localization as 3T3 and 3T6 fibroblasts grow to confluency and quiescence: a role for phosphorylation at ser-729?
AuthorsEngland K, Rumsby MG
JournalBiochem J
PubMed ID11062054
Protein kinase C (PKC) epsilon in 3T3 and 3T6 fibroblasts and in C6 glioma cells migrated on SDS/PAGE predominantly as a doublet with molecular masses of 87 and 95 kDa (PKC epsilon(87) and PKC epsilon(95) respectively). PKC epsilon(95) predominates when cells reach confluency but PKC epsilon(87) was the main form ... More
Ethanol causes translocation of cAMP-dependent protein kinase catalytic subunit to the nucleus.
AuthorsDohrman DP, Diamond I, Gordon AS
JournalProc Natl Acad Sci U S A
PubMed ID8816779
Short- and long-term ethanol exposures have been shown to alter cellular levels of cAMP, but little is known about the effects of ethanol on cAMP-dependent protein kinase (PKA). When cAMP levels increase, the catalytic subunit of PKA (C alpha) is released from the regulatory subunit, phosphorylates nearby proteins, and then ... More
Stage-specific expression and targeting of cyst wall protein-green fluorescent protein chimeras in Giardia.
AuthorsHehl AB, Marti M, Köhler P
JournalMol Biol Cell
PubMed ID10793152
In preparation for being shed into the environment as infectious cysts, trophozoites of Giardia spp. synthesize and deposit large amounts of extracellular matrix into a resistant extracellular cyst wall. Functional aspects of this developmentally regulated process were investigated by expressing a series of chimeric cyst wall protein 1 (CWP1)-green fluorescent ... More
Enhancement of migration by protein kinase Calpha and inhibition of proliferation and cell cycle progression by protein kinase Cdelta in capillary endothelial cells.
AuthorsHarrington EO, Löffler J, Nelson PR, Kent KC, Simons M, Ware JA
JournalJ Biol Chem
PubMed ID9054439
Activation of protein kinase C (PKC) induces angiogenesis, migration, and proliferation of endothelial cells (EC), but can also prevent growth factor-induced EC proliferation. To determine whether these disparate effects are mediated by substrates of individual PKC isoenzymes, PKCalpha and PKCdelta were overexpressed in rat microvascular EC. Basal and stimulated migration ... More
Increased membrane affinity of the C1 domain of protein kinase Cdelta compensates for the lack of involvement of its C2 domain in membrane recruitment.
AuthorsGiorgione JR, Lin JH, McCammon JA, Newton AC
JournalJ Biol Chem
PubMed ID16293612
Protein kinase C (PKC) family members are allosterically activated following membrane recruitment by specific membrane-targeting modules. Conventional PKC isozymes are recruited to membranes by two such modules: a C1 domain, which binds diacylglycerol (DAG), and a C2 domain, which is a Ca2+-triggered phospholipid-binding module. In contrast, novel PKC isozymes respond ... More
The signal sequence of exported protein-1 directs the green fluorescent protein to the parasitophorous vacuole of transfected malaria parasites.
AuthorsAdisa A, Rug M, Klonis N, Foley M, Cowman AF, Tilley L
JournalJ Biol Chem
PubMed ID12456681
The malaria parasite, Plasmodium falciparum, spends part of its life cycle inside the erythrocytes of its human host. In the mature stages of intraerythrocytic growth, the parasite undertakes extensive remodeling of its adopted cellular home by exporting proteins beyond the confines of its own plasma membrane. To examine the signals ... More
Differential localization of Rho GTPases in live cells: regulation by hypervariable regions and RhoGDI binding.
Determinants of membrane targeting of Rho proteins were investigated in live cells with green fluorescent fusion proteins expressed with or without Rho-guanine nucleotide dissociation inhibitor (GDI)alpha. The hypervariable region determined to which membrane compartment each protein was targeted. Targeting was regulated by binding to RhoGDI alpha in the case of ... More
Lipopolysaccharide internalization activates endotoxin-dependent signal transduction in cardiomyocytes.
AuthorsCowan DB, Noria S, Stamm C, Garcia LM, Poutias DN, del Nido PJ, McGowan FX
JournalCirc Res
PubMed ID11249872
We tested the hypothesis that bacterial lipopolysaccharide (LPS) must be internalized to facilitate endotoxin-dependent signal activation in cardiac myocytes. Fluorescently labeled LPS was used to treat primary cardiomyocyte cultures, perfused heart preparations, and the RAW264.7 macrophage cell line. Using confocal microscopy and spectrofluorometry, we found that LPS was rapidly internalized ... More
Functional roles for fatty acylated amino-terminal domains in subcellular localization.
AuthorsMcCabe JB, Berthiaume LG
JournalMol Biol Cell
PubMed ID10564270
Several membrane-associating signals, including covalently linked fatty acids, are found in various combinations at the N termini of signaling proteins. The function of these combinations was investigated by appending fatty acylated N-terminal sequences to green fluorescent protein (GFP). Myristoylated plus mono/dipalmitoylated GFP chimeras and a GFP chimera containing a myristoylated ... More
Intracellular delivery of phosphoinositides and inositol phosphates using polyamine carriers.
Phosphoinositide signaling regulates events in endocytosis and exocytosis, vesicular trafficking of proteins, transduction of extracellular signals, remodeling of the actin cytoskeleton, regulation of calcium flux, and apoptosis. Obtaining mechanistic insights in living cells is impeded by the membrane impermeability of these anionic lipids. We describe a carrier system for intracellular ... More
Expression of wild type and mutant ELOVL4 in cell culture: subcellular localization and cell viability.
AuthorsKaran G, Yang Z, Zhang K
JournalMol Vis
PubMed ID15073583
PURPOSE: ELOVL4 is a member of the fatty acid elongase (ELO) family of genes. Mutations of this gene are responsible for autosomal dominant Stargardt-like macular degeneration. However, the specific role of ELOVL4 in photoreceptor cells and the mechanism by which mutations in ELOVL4 causes macular degeneration are not known. In ... More
Secretory group IIA phospholipase A(2) generates anti-apoptotic survival signals in kidney fibroblasts.
AuthorsZhang Y, Lemasters J, Herman B
JournalJ Biol Chem
PubMed ID10488115
Mammalian group IIA phospholipase A(2) (PLA(2)) is believed to play important roles in inflammation, cell injury, and tumor resistance. However, the cellular site of action has not been clearly defined as it has long been recognized that group IIA PLA(2) is both a secretory and mitochondrial protein. The purpose of ... More
Phosphatidylinositol 4,5-bisphosphate induces actin-based movement of raft-enriched vesicles through WASP-Arp2/3.
AuthorsRozelle AL, Machesky LM, Yamamoto M, Driessens MH, Insall RH, Roth MG, Luby-Phelps K, Marriott G, Hall A, Yin HL
JournalCurr Biol
PubMed ID10744973
BACKGROUND: Phosphatidylinositol 4,5-bisphosphate (PIP(2)) has been implicated in the regulation of the actin cytoskeleton and vesicle trafficking. It stimulates de novo actin polymerization by activating the pathway involving the Wiskott-Aldrich syndrome protein (WASP) and the actin-related protein complex Arp2/3. Other studies show that actin polymerizes from cholesterol-sphingolipid-rich membrane microdomains called ... More
Cell-cycle and developmental regulation of TbRAB31 localisation, a GTP-locked Rab protein from Trypanosoma brucei.
AuthorsField H, Sherwin T, Smith AC, Gull K, Field MC
JournalMol Biochem Parasitol
PubMed ID10743608
Rab proteins are small GTPases that control the direction and timing of vesicle fusion during intracellular trafficking between membraneous compartments. Genome sequencing and EST analysis of Trypanosoma brucei indicates that the trypanosome Rab (TbRAB) gene family, and hence complexity of intracellular transport pathways, is intermediate between Saccharomyces cerevisiae and mammals. ... More
Expression of a functional Drosophila melanogaster CMP-sialic acid synthetase. Differential localization of the Drosophila and human enzymes.
AuthorsViswanathan K, Tomiya N, Park J, Singh S, Lee YC, Palter K, Betenbaugh MJ
JournalJ Biol Chem
PubMed ID16537535
CMP-N-acetylneuraminic acid is a critical metabolite in the generation of glycoconjugates that play a role in development and other physiological processes. Whereas pathways for its generation are firmly established in vertebrates, the presence and function of the relevant synthetic enzyme in insects and other protostomes is unknown. In this study, ... More
Trafficking of spontaneously endocytosed MHC proteins.
AuthorsChiu I, Davis DM, Strominger JL
JournalProc Natl Acad Sci U S A
PubMed ID10570178
Class I MHC protein primarily presents endogenous antigen but also may present exogenous antigen. Here, we investigated the intracellular pathway of spontaneously internalized class I MHC protein by confocal microscopy. beta(2)-microglobulin (beta(2)m), labeled with a single fluorophore, was exchanged at the surface of B cell transfectants to specifically mark cell ... More
Differential localization of protein kinase C delta by phorbol esters and related compounds using a fusion protein with green fluorescent protein.
Enzyme localization often plays a controlling role in determining its activity and specificity. Protein kinase C (PKC) has long been known to translocate in response to physiological stimuli as well as to exogenous ligands such as the phorbol esters. We report here that different phorbol derivatives and related ligands, selected ... More
Tracing transgene expression in living zebrafish embryos.
AuthorsKöster RW, Fraser SE
JournalDev Biol
PubMed ID11336499
Ectopic expression by injection of plasmid DNA is rarely used in zebrafish embryos due to a low frequency of cells expressing a transgene of interest at detectable levels. Furthermore, the mosaic nature of ectopic expression by plasmid injection requires the direct detection of transgene-expressing cells. We have used the transcriptional ... More
ClC-3 chloride channels facilitate endosomal acidification and chloride accumulation.
AuthorsHara-Chikuma M, Yang B, Sonawane ND, Sasaki S, Uchida S, Verkman AS
JournalJ Biol Chem
PubMed ID15504734
We investigated the involvement of ClC-3 chloride channels in endosomal acidification by measurement of endosomal pH and chloride concentration [Cl-] in control versus ClC-3-deficient hepatocytes and in control versus ClC-3-transfected Chinese hamster ovary cells. Endosomes were labeled with pH or [Cl-]-sensing fluorescent transferrin (Tf), which targets to early/recycling endosomes, or ... More
Phorbol esters and related analogs regulate the subcellular localization of beta 2-chimaerin, a non-protein kinase C phorbol ester receptor.
AuthorsCaloca MJ, Wang H, Delemos A, Wang S, Kazanietz MG
JournalJ Biol Chem
PubMed ID11278894
The novel phorbol ester receptor beta2-chimaerin is a Rac-GAP protein possessing a single copy of the C1 domain, a 50-amino acid motif initially identified in protein kinase C (PKC) isozymes that is involved in phorbol ester and diacylglycerol binding. We have previously shown that, like PKCs, beta2-chimaerin binds phorbol esters ... More
Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway.
Authors
JournalNat Commun
PubMed ID31980602
Enhanced Loading of Functional miRNA Cargo via pH Gradient Modification of Extracellular Vesicles.
Authors
JournalMol Ther
PubMed ID31911034
Tr-milRNA1 Contributes to Lignocellulase Secretion under Heat Stress by Regulating the Lectin-Type Cargo Receptor Gene Trvip36 in Trichoderma guizhouence NJAU 4742.
Authors
JournalJ Fungi (Basel)
PubMed ID34946980
The Phenotypic Effects of Exosomes Secreted from Distinct Cellular Sources: a Comparative Study Based on miRNA Composition.
Authors
JournalAAPS J
PubMed ID29713834
Visualizing Alzheimer's Disease Mouse Brain with Multispectral Optoacoustic Tomography using a Fluorescent probe, CDnir7.
Authors
JournalSci Rep
PubMed ID31427599
Unusual Organization of I-BAR Proteins on Tubular and Vesicular Membranes.
Expansion Microscopy Reveals Plasmodium falciparum Blood-Stage Parasites Undergo Anaphase with A Chromatin Bridge in the Absence of Mini-Chromosome Maintenance Complex Binding Protein.
Authors
JournalMicroorganisms
PubMed ID34835432
Friction Mediates Scission of Tubular Membranes Scaffolded by BAR Proteins.
Authors
JournalCell
PubMed ID28648660
Arrest of nuclear division in Plasmodium through blockage of erythrocyte surface exposed ribosomal protein P2.