Staurosporine, a non-specific PKC inhibitor, induces keratinocyte differentiation and raises intracellular calcium, but Ro31-8220, a specific inhibitor, does not.
AuthorsJones KT, Sharpe GR
JournalJ Cell Physiol
PubMed ID7512976
The responsiveness of normal human keratinocytes to different modulators of protein kinase C (PKC) was investigated. The PKC agonist TPA, staurosporine (a non-specific inhibitor), and Ro31-8220 (a specific inhibitor) were studied for effect on cell morphology, growth rate, involucrin expression, and intracellular calcium levels. Surprisingly the response to nanomolar concentrations ... More
Rapid, endoplasmic reticulum-independent diffusion of the mitotic Golgi haze.
AuthorsAxelsson MA, Warren G
JournalMol Biol Cell
PubMed ID14767069
Early in mitosis, the mammalian Golgi apparatus disassembles, and fluorescence microscopy reveals Golgi clusters and an extensive, nonresolvable haze that either represents scattered vesicles or a merged endoplasmic reticulum (ER)-Golgi compartment. To help decide between these alternatives, we have carried out a combined microscopic and pharmacological analysis, by using a ... More
Expression of wild type and mutant ELOVL4 in cell culture: subcellular localization and cell viability.
AuthorsKaran G, Yang Z, Zhang K
JournalMol Vis
PubMed ID15073583
PURPOSE: ELOVL4 is a member of the fatty acid elongase (ELO) family of genes. Mutations of this gene are responsible for autosomal dominant Stargardt-like macular degeneration. However, the specific role of ELOVL4 in photoreceptor cells and the mechanism by which mutations in ELOVL4 causes macular degeneration are not known. In ... More