Synthesis of fluorescent substrates and their application to study of sphingolipid metabolism in vitro and in intact cells.
AuthorsDagan A, Agmon V, Gatt S, Dinur T
JournalMethods Enzymol
PubMed ID11070879
Glycosphingolipid synthesis requires FAPP2 transfer of glucosylceramide.
AuthorsD'Angelo G, Polishchuk E, Di Tullio G, Santoro M, Di Campli A, Godi A, West G, Bielawski J, Chuang CC, van der Spoel AC, Platt FM, Hannun YA, Polishchuk R, Mattjus P, De Matteis MA,
JournalNature
PubMed ID17687330
The molecular machinery responsible for the generation of transport carriers moving from the Golgi complex to the plasma membrane relies on a tight interplay between proteins and lipids. Among the lipid-binding proteins of this machinery, we previously identified the four-phosphate adaptor protein FAPP2, the pleckstrin homology domain of which binds ... More