Simultaneous measurement of RBC velocity, flux, hematocrit and shear rate in vascular networks.
AuthorsKamoun WS, Chae SS, Lacorre DA, Tyrrell JA, Mitre M, Gillissen MA, Fukumura D, Jain RK, Munn LL,
JournalNat Methods
PubMed ID20581828
Not all tumor vessels are equal. Tumor-associated vasculature includes immature vessels, regressing vessels, transport vessels undergoing arteriogenesis and peritumor vessels influenced by tumor growth factors. Current techniques for analyzing tumor blood flow do not discriminate between vessel subtypes and only measure average changes from a population of dissimilar vessels. We ... More
Dynamics of a chemoattractant receptor in living neutrophils during chemotaxis.
AuthorsServant G, Weiner OD, Neptune ER, Sedat JW, Bourne HR
JournalMol Biol Cell
PubMed ID10198064
'Persistent directional movement of neutrophils in shallow chemotactic gradients raises the possibility that cells can increase their sensitivity to the chemotactic signal at the front, relative to the back. Redistribution of chemoattractant receptors to the anterior pole of a polarized neutrophil could impose asymmetric sensitivity by increasing the relative strength ... More
Regulation of C-cadherin function during activin induced morphogenesis of Xenopus animal caps.
AuthorsBrieher WM, Gumbiner BM
JournalJ Cell Biol
PubMed ID8034750
'Treatment of Xenopus animal pole tissue with activin results in the induction of mesodermal cell types and a dramatic elongation of the tissue. The morphogenetic movements involved in the elongation appear similar to those in normal gastrulation, which is driven by cell rearrangement and cell intercalations. We have used this ... More
Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy.
AuthorsEvans CL, Potma EO, Puoris'haag M, Côté D, Lin CP, Xie XS
JournalProc Natl Acad Sci U S A
PubMed ID16263923
'Imaging living organisms with molecular selectivity typically requires the introduction of specific labels. Many applications in biology and medicine, however, would significantly benefit from a noninvasive imaging technique that circumvents such exogenous probes. In vivo microscopy based on vibrational spectroscopic contrast offers a unique approach for visualizing tissue architecture with ... More
In vivo migration of dendritic cells differentiated in vitro: a chimpanzee model.
AuthorsBarratt-Boyes SM, Watkins SC, Finn OJ
JournalJ Immunol
PubMed ID9144465
'Dendritic cells with potent Ag-presenting function can be propagated from peripheral blood using recombinant cytokines, and these cells have potential usefulness as immunotherapeutic agents in the treatment of cancer and other disease states. However, it is not known if these in vitro differentiated dendritic cells have the capacity to migrate ... More
'Array-based sensors provide an architecture for multianalyte sensing. In this paper, we report a new approach for array fabrication. Sensors are made by immobilizing different reactive chemistries on the surfaces of microspheres. Sensor arrays are prepared by randomly distributing a mixture of microsphere sensors on an optical substrate containing thousands ... More
Cell adhesion profiling using extracellular matrix protein microarrays.
AuthorsKuschel C, Steuer H, Maurer AN, Kanzok B, Stoop R, Angres B
JournalBiotechniques
PubMed ID16629399
'We have developed a microarray-based system for cell adhesion profiling of large panels of cell-adhesive proteins to increase the throughput of in vitro cell adhesion assays, which are currently primarily performed in multiwell plates. Miniaturizing cell adhesion assays to an array format required the development of protocols for the reproducible ... More
Rapid labeling of neuronal populations by ballistic delivery of fluorescent dyes.
AuthorsGrutzendler J, Tsai J, Gan WB
JournalMethods
PubMed ID12695105
'Particle-mediated ballistic delivery of fluorescent dyes has been recently used to label neuronal populations in a rapid and efficient fashion. Here we describe detailed protocols for this technique as well as recent improvements in its implementation. This technique allows rapid labeling of entire neurons in a Golgi-like manner after membranes ... More
In vivo calcium elevations in thymocytes with T cell receptors that are specific for self ligands.
AuthorsNakayama T, Ueda Y, Yamada H, Shores EW, Singer A, June CH
JournalScience
PubMed ID1621102
'Selection of the T cell receptor (TCR) repertoire in the thymus probably involves TCR-mediated signals transduced in developing thymocytes after interaction with thymic stromal cells bearing self ligands. TCR-transduced signals should have identifiable consequences that would distinguish thymocytes whose TCRs have been engaged by self ligands from those whose TCRs ... More
Microenvironmental contaminations induced by fluorescent lipophilic dyes used for noninvasive in vitro and in vivo cell tracking.
AuthorsLassailly F, Griessinger E, Bonnet D,
JournalBlood
PubMed ID20215639
'Determining how normal and leukemic stem cells behave in vivo, in a dynamic and noninvasive way, remains a major challenge. Most optical tracking technologies rely on the use of fluorescent or bioluminescent reporter genes, which need to be stably expressed in the cells of interest. Because gene transfer in primary ... More
Human erythrocytes adhering to schistosomula of Schistosoma mansoni lyse and fail to transfer membrane components to the parasite.
AuthorsCaulfield JP, Cianci CM
JournalJ Cell Biol
PubMed ID4008525
'We studied the adherence of human erythrocytes to larvae of the intravascular parasite Schistosoma mansoni by transmission microscopy, freeze fracture, and fluorescence techniques. In addition, we used the adherent cells to investigate the problem of host antigen acquisition. Schistosomula were cultured for from 24 to 48 h after transformation in ... More
Physical interaction between dendritic cells and tumor cells results in an immunogen that induces protective and therapeutic tumor rejection.
AuthorsCelluzzi CM, Falo LD
JournalJ Immunol
PubMed ID9531260
'Dendritic cells (DCs) are potent professional APCs capable of presenting Ag in the context of costimulatory signals necessary for T cell activation. Although tumor cells express target Ags, they are generally incapable of stimulating an immune response. We show that the short term physical interaction of DCs and tumor cells, ... More
Fluorescent probe partitioning in GUVs of binary phospholipid mixtures: implications for interpreting phase behavior.
AuthorsJuhasz J, Davis JH, Sharom FJ
JournalBiochim Biophys Acta
PubMed ID21945563
'The phase behavior of membrane lipids is known to influence the organization and function of many integral proteins. Giant unilamellar vesicles (GUVs) provide a very useful model system in which to examine the details of lipid phase separation using fluorescence imaging. The visualization of domains in GUVs of binary and ... More
Carbocyanine dyes with long alkyl side-chains: broad spectrum inhibitors of mitochondrial electron transport chain activity.
AuthorsAnderson WM, Trgovcich-Zacok D
JournalBiochem Pharmacol
PubMed ID7763312
'Certain indocarbocyanine, thiacarbocyanine, and oxacarbocyanine dyes possessing short alkyl side-chains (one to five carbons) are potent inhibitors of mammalian mitochondrial NADH-ubiquinone reductase (EC 1.6.99.3) activity (Anderson et al., Biochem Pharmacol 41: 677-684, 1991; Anderson et al., Biochem Pharmacol 45: 691-696, 1993; Anderson et al., Biochem Pharmacol 45: 2115-2122, 1993), and ... More
Removal of chylomicron remnants in transgenic mice overexpressing normal and membrane-anchored hepatic lipase.
AuthorsLee SJ, Kadambi S, Yu KC, David C, Azhar S, Cooper AD, Choi SY
JournalJ Lipid Res
PubMed ID15520453
'The LDL receptor and the LDL receptor-related protein (LRP) mediate the removal of chylomicron remnants. The LRP pathway involves sequestration of particles in the space of Disse. It has been proposed that either alone or in combination with other factors, such as apolipoprotein E and proteoglycans, hepatic lipase (HL) may ... More
Ceramide triggers budding of exosome vesicles into multivesicular endosomes.
'Intraluminal vesicles of multivesicular endosomes are either sorted for cargo degradation into lysosomes or secreted as exosomes into the extracellular milieu. The mechanisms underlying the sorting of membrane into the different populations of intraluminal vesicles are unknown. Here, we find that cargo is segregated into distinct subdomains on the endosomal ... More
Multicolor "DiOlistic" labeling of the nervous system using lipophilic dye combinations.
AuthorsGan WB, Grutzendler J, Wong WT, Wong RO, Lichtman JW
JournalNeuron
PubMed ID10985343
'We describe a technique for rapid labeling of a large number of cells in the nervous system with many different colors. By delivering lipophilic dye-coated particles to neuronal preparations with a "gene gun," individual neurons and glia whose membranes are contacted by the particles are quickly labeled. Using particles that ... More
Growth cone and dendrite dynamics in zebrafish embryos: early events in synaptogenesis imaged in vivo.
AuthorsJontes JD, Buchanan J, Smith SJ
JournalNat Neurosci
PubMed ID10700254
'We used time-lapse fluorescence microscopy to observe the growth of Mauthner cell axons and their postsynaptic targets, the primary motor neurons, in spinal cords of developing zebrafish embryos. Upon reaching successive motor neurons, the Mauthner growth cone paused briefly before continuing along its path. Varicosities formed at regular intervals and ... More
The first cleavage of the mouse zygote predicts the blastocyst axis.
AuthorsPlusa B, Hadjantonakis AK, Gray D, Piotrowska-Nitsche K, Jedrusik A, Papaioannou VE, Glover DM, Zernicka-Goetz M
JournalNature
PubMed ID15772664
'One of the unanswered questions in mammalian development is how the embryonic-abembryonic axis of the blastocyst is first established. It is possible that the first cleavage division contributes to this process, because in most mouse embryos the progeny of one two-cell blastomere primarily populate the embryonic part of the blastocyst ... More
Timing and topography of nucleus magnocellularis innervation by the cochlear ganglion.
AuthorsMolea D, Rubel EW
JournalJ Comp Neurol
PubMed ID14566951
'This series of experiments examined the arrival and organization of cochlear nerve axons in the primary auditory brainstem nucleus, nucleus magnocellularis (NM), of the chick. DiI and DiD were injected into the cochlear nerve, cochlear ganglion, and basilar papilla (i.e., avian cochlea) in fixed tissue and labeled axons were studied ... More
Embryonic origins of auditory brain-stem nuclei in the chick hindbrain.
AuthorsCramer KS, Fraser SE, Rubel EW
JournalDev Biol
PubMed ID10926755
'The auditory nuclei of the chick brain stem have distinct morphologies and highly specific synaptic connectivity. Nucleus magnocellularis (NM) and nucleus angularis receive tonotopically ordered cochlear input. NM in turn projects tonotopically to nucleus laminaris (NL), maintaining binaural specificity with projections to either dorsal or ventral NL dendrites. NM and ... More
Ligands for clathrin-mediated endocytosis are differentially sorted into distinct populations of early endosomes.
AuthorsLakadamyali M, Rust MJ, Zhuang X
JournalCell
PubMed ID16530046
'Cells rely on the correct sorting of endocytic ligands and receptors for proper function. Early endosomes have been considered as the initial sorting station where cargos for degradation separate from those for recycling. Using live-cell imaging to monitor individual endosomes and ligand particles in real time, we have discovered a ... More
Influence of hydrophobic mismatch and amino acid composition on the lateral diffusion of transmembrane peptides.
'We investigated the effect of amino acid composition and hydrophobic length of alpha-helical transmembrane peptides and the role of electrostatic interactions on the lateral diffusion of the peptides in lipid membranes. Model peptides of varying length and composition, and either tryptophans or lysines as flanking residues, were synthesized. The peptides ... More
Fibre optic sensor for the detection of potassium using fluorescence energy transfer.
AuthorsRoe JN, Szoka FC, Verkman AS
JournalAnalyst
PubMed ID2363517
'A fluorescence fibre optic sensor has been developed for measurement of the potassium concentration in aqueous solution based on the change in optical absorbance of the hydrophobic indicator 7-decyl-2-methyl-4-(3'',5''-dichlorophen-4''-one)indonaphth-1-o l (MEDPIN). The sensor was constructed by dipping the distal end of a single optical fibre in a poly(vinyl chloride) (PVC) ... More
Maturation and trafficking of monocyte-derived dendritic cells in monkeys: implications for dendritic cell-based vaccines.
AuthorsBarratt-Boyes SM, Zimmer MI, Harshyne LA, Meyer EM, Watkins SC, Capuano S, Murphey-Corb M, Falo LD, Donnenberg AD
JournalJ Immunol
PubMed ID10679086
'Human dendritic cells (DC) have polarized responses to chemokines as a function of maturation state, but the effect of maturation on DC trafficking in vivo is not known. We have addressed this question in a highly relevant rhesus macaque model. We demonstrate that immature and CD40 ligand-matured monocyte-derived DC have ... More
Iontophoretic dye labeling of embryonic cells.
AuthorsFraser SE
JournalMethods Cell Biol
PubMed ID8722475
Tech.Sight. Optical sectioning--slices of life.
AuthorsPaddock S
JournalScience
PubMed ID11847348
Temperature transitions of protein properties in human red blood cells.
AuthorsArtmann GM, Kelemen C, Porst D, Büldt G, Chien S
JournalBiophys J
PubMed ID9826638
Human red blood cells (RBC) undergo a sudden change from blocking to passing through 1.3 +/- 0.2-micrometer micropipettes at a transition temperature (Tc) of 36.4 degrees C. For resealed RBC ghosts this transition occurs at 28.3 degrees C (Tg). These findings are attributed to an elastomeric transition of hemoglobin from ... More
Adaptability, sensitivity, resolution and non-invasiveness are the attributes that have contributed to the longstanding use of light as an investigational tool and form the basis of optical imaging (OI). OI, which encompasses numerous techniques and methods, is rapid (<5 min), inexpensive, noninvasive, nontoxic (no radiation) and has molecular (single-cell) sensitivity, ... More
Cyanine-loaded lipid nanoparticles for improved in vivo fluorescence imaging.
AuthorsTexier I, Goutayer M, Da Silva A, Guyon L, Djaker N, Josserand V, Neumann E, Bibette J, Vinet F,
JournalJ Biomed Opt
PubMed ID19895107
Fluorescence is a very promising radioactive-free technique for functional imaging in small animals and, in the future, in humans. However, most commercial near-infrared dyes display poor optical properties, such as low fluorescence quantum yields and short fluorescence lifetimes. In this paper, we explore whether the encapsulation of infrared cyanine dyes ... More
Effect of line tension on the lateral organization of lipid membranes.
AuthorsGarcía-Sáez AJ, Chiantia S, Schwille P,
JournalJ Biol Chem
PubMed ID17848582
The principles of organization and functioning of cellular membranes are currently not well understood. The raft hypothesis suggests the existence of domains or rafts in cell membranes, which behave as protein and lipid platforms. They have a functional role in important cellular processes, like protein sorting or cell signaling, among ... More
Diolistics: incorporating fluorescent dyes into biological samples using a gene gun.
AuthorsO'Brien JA, Lummis SC,
JournalTrends Biotechnol
PubMed ID17945370
The hand-held gene gun provides a rapid and efficient method of incorporating fluorescent dyes into cells, a technique that is becoming known as diolistics. Transporting fluorescent dyes into cells has, in the past, used predominantly injection or chemical methods. The use of the gene gun, combined with the new generation ... More
How curved membranes recruit amphipathic helices and protein anchoring motifs.
Lipids and several specialized proteins are thought to be able to sense the curvature of membranes (MC). Here we used quantitative fluorescence microscopy to measure curvature-selective binding of amphipathic motifs on single liposomes 50-700 nm in diameter. Our results revealed that sensing is predominantly mediated by a higher density of ... More
Suitability of various membrane lipophilic probes for the detection of trogocytosis by flow cytometry.
AuthorsDaubeuf S, Bordier C, Hudrisier D, Joly E,
JournalCytometry A
PubMed ID19051238
Trogocytosis is a recently discovered phenomenon whereby lymphocytes capture fragments of the plasma membrane from antigen presenting cells (APCs). Using APCs labeled with widely used fluorescent lipophilic probes, we previously described a trogocytosis analysis protocol (TRAP) useful to understand the mechanisms and biological consequences of this process and to identify ... More
Rab conversion as a mechanism of progression from early to late endosomes.
AuthorsRink J, Ghigo E, Kalaidzidis Y, Zerial M
JournalCell
PubMed ID16143105
The mechanisms of endosome biogenesis and maintenance are largely unknown. The small GTPases Rab 5 and Rab 7 are key determinants of early and late endosomes, organizing effector proteins into specific membrane subdomains. Whether such Rab machineries are indefinitely maintained on membranes or can disassemble in the course of cargo ... More
Use of a night vision intensifier for direct visualization by eye of far-red and near-infrared fluorescence through an optical microscope.
AuthorsSiddiqi MA, Kilduff GM, Gearhart JD
JournalJ Microsc
PubMed ID14629562
We describe the design, construction and testing of a prototype device that allows the direct visualization by eye of far-red and near-infrared (NIR) fluorescence through an optical microscope. The device incorporates a gallium arsenide (GaAs) image intensifier, typically utilized in low-light or 'night vision' applications. The intensifier converts far-red and ... More
Agonist-induced PIP(2) hydrolysis inhibits cortical actin dynamics: regulation at a global but not at a micrometer scale.
Authorsvan Rheenen J, Jalink K
JournalMol Biol Cell
PubMed ID12221130
Phosphatidylinositol 4, 5-bisphosphate (PIP(2)) at the inner leaflet of the plasma membrane has been proposed to locally regulate the actin cytoskeleton. Indeed, recent studies that use GFP-tagged pleckstrin homology domains (GFP-PH) as fluorescent PIP(2) sensors suggest that this lipid is enriched in membrane microdomains. Here we report that this concept ... More
PI(4,5)P2-dependent microdomain assemblies capture microtubules to promote and control leading edge motility.
AuthorsGolub T, Caroni P
JournalJ Cell Biol
PubMed ID15809307
The lipid second messenger PI(4,5)P(2) modulates actin dynamics, and its local accumulation at plasmalemmal microdomains (rafts) might mediate regulation of protrusive motility. However, how PI(4,5)P(2)-rich rafts regulate surface motility is not well understood. Here, we show that upon signals promoting cell surface motility, PI(4,5)P(2) directs the assembly of dynamic raft-rich ... More
A potassium channel-MiRP complex controls neurosensory function in Caenorhabditis elegans.
AuthorsBianchi L, Kwok SM, Driscoll M, Sesti F
JournalJ Biol Chem
PubMed ID12533541
MinK-related peptides (MiRPs) are single transmembrane proteins that associate with mammalian voltage-gated K(+) subunits. Here we report the cloning and functional characterization of a MiRP beta-subunit, MPS-1, and of a voltage-gated pore-forming potassium subunit, KVS-1, from the nematode Caenorhabditis elegans. mps-1 is expressed in chemosensory and mechanosensory neurons and co-localizes ... More
Multiple intermediates in SNARE-induced membrane fusion.
AuthorsYoon TY, Okumus B, Zhang F, Shin YK, Ha T
JournalProc Natl Acad Sci U S A
PubMed ID17167056
Membrane fusion in eukaryotic cells is thought to be mediated by a highly conserved family of proteins called SNAREs (soluble N-ethyl maleimide sensitive-factor attachment protein receptors). The vesicle-associated v-SNARE engages with its partner t-SNAREs on the target membrane to form a coiled coil that bridges two membranes and facilitates fusion. ... More
Furrow-associated microtubule arrays are required for the cohesion of zebrafish blastomeres following cytokinesis.
AuthorsJesuthasan S
JournalJ Cell Sci
PubMed ID9819360
During the first few cleavages of the zebrafish embryo, daughter blastomeres are loosely associated immediately after furrow ingression, but then gradually cohere. Cohesion appears to be cadherin-dependent, as cadherin and beta-catenin are found at the membrane between cohering blastomeres, and blastomeres fail to cohere in calcium-free medium. Cadherin and beta-catenin ... More
Heterogeneity of freshly isolated human tonsil dendritic cells demonstrated by intracellular markers, phagocytosis, and membrane dye transfer.
AuthorsStent G, Reece JC, Baylis DC, Ivinson K, Paukovics G, Thomson M, Cameron PU
JournalCytometry
PubMed ID12116363
BACKGROUND: Heterogeneity within human dendritic cells (DCs) has been described but its functional relationships to cells of macrophage lineage and its role in human immunodeficiency virus (HIV) infection in vivo remain unclear. METHODS: Tonsil macrophages and DCs were isolated from low-density cells by negative selection and DCs were sorted into ... More
DiOLISTIC labeling of neurons from rodent and non-human primate brain slices.
AuthorsSeabold GK, Daunais JB, Rau A, Grant KA, Alvarez VA,
JournalJ Vis Exp
PubMed ID20644510
DiOLISTIC staining uses the gene gun to introduce fluorescent dyes, such as DiI, into neurons of brain slices (Gan et al., 2009; O'Brien and Lummis, 2007; Gan et al., 2000). Here we provide a detailed description of each step required together with exemplary images of good and bad outcomes that ... More
Liposomal clodronate as a novel agent for treating autoimmune hemolytic anemia in a mouse model.
AuthorsJordan MB, van Rooijen N, Izui S, Kappler J, Marrack P
JournalBlood
PubMed ID12393630
Autoimmune hemolytic anemia (AIHA) is a disease in which autoantibodies against red blood cells (RBCs) lead to their premature destruction. Most clinically significant autoantibodies are of the immunoglobulin G (IgG) type, which leads primarily to the uptake and destruction of RBCs by splenic and hepatic macrophages. Therapies such as corticosteroids ... More
Photodamage to intact erythrocyte membranes at high laser intensities: methods of assay and suppression.
AuthorsBloom JA, Webb WW
JournalJ Histochem Cytochem
PubMed ID6725935
A simple hemolytic assay of the photodamage suffered by individual intact erythrocytes upon localized exposure to high laser intensities such as those encountered in fluorescence photobleaching recovery (FPR) experiments has been characterized. At incident beam powers over 100,000 W/cm2 at 514 or 568 nm, hemoglobin absorption induces thermal-shock lysis. Below ... More
Lipid diffusibility in the intact erythrocyte membrane.
AuthorsBloom JA, Webb WW
JournalBiophys J
PubMed ID6603237
The lateral diffusion of fluorescent lipid analogues in the plasma membrane of intact erythrocytes from man, mouse, rabbit, and frog has been measured by fluorescence photobleaching recovery (FPR). Intact cells from dystrophic, normoblastic, hemolytic, and spherocytotic mouse mutants; from hypercholesterolemic rabbits and humans; and from prenatal, neonatal, and juvenile mice ... More
In vivo imaging of specialized bone marrow endothelial microdomains for tumour engraftment.
AuthorsSipkins DA, Wei X, Wu JW, Runnels JM, Côté D, Means TK, Luster AD, Scadden DT, Lin CP
JournalNature
PubMed ID15959517
The organization of cellular niches is known to have a key role in regulating normal stem cell differentiation and regeneration, but relatively little is known about the architecture of microenvironments that support malignant metastasis. Using dynamic in vivo confocal imaging, here we show that murine bone marrow contains unique anatomic ... More
Dynamic behavior of rod photoreceptor disks.
AuthorsChen C, Jiang Y, Koutalos Y
JournalBiophys J
PubMed ID12202366
Eukaryotic cells use membrane organelles, like the endoplasmic reticulum or the Golgi, to carry out different functions. Vertebrate rod photoreceptors use hundreds of membrane sacs (the disks) for the detection of light. We have used fluorescent tracers and single cell imaging to study the properties of rod photoreceptor disks. Labeling ... More
Microtubule-dependent movement of late endocytic vesicles in vitro: requirements for Dynein and Kinesin.
AuthorsBananis E, Nath S, Gordon K, Satir P, Stockert RJ, Murray JW, Wolkoff AW
JournalMol Biol Cell
PubMed ID15181154
Our previous studies demonstrated that fluorescent early endocytic vesicles prepared from rat liver after injection of Texas red asialoorosomucoid contain asialoglycoprotein and its receptor and move and undergo fission along microtubules using kinesin I and KIFC2, with Rab4 regulating KIFC2 activity (J. Cell Sci. 116, 2749, 2003). In the current ... More
Novel E-cadherin-mediated adhesion in peripheral nerve: Schwann cell architecture is stabilized by autotypic adherens junctions.
AuthorsFannon AM, Sherman DL, Ilyina-Gragerova G, Brophy PJ, Friedrich VL, Colman DR
JournalJ Cell Biol
PubMed ID7698985
Previous studies (Blank, W. F., M. B. Bunge, and R. P. Bunge. 1974. Brain Res. 67:503-518) showed that Schwann cell paranodal membranes were disrupted in calcium free medium suggesting that cadherin mediated mechanisms may operate to maintain the integrity of the paranodal membrane complex. Using antibodies against the fifth extracellular ... More
Regulation of neural markers nestin and GFAP expression by cultivated bone marrow stromal cells.
AuthorsWislet-Gendebien S, Leprince P, Moonen G, Rogister B
JournalJ Cell Sci
PubMed ID12840074
Bone marrow stromal cells can differentiate into many types of mesenchymal cells, i.e. osteocyte, chondrocyte and adipocyte, but can also differentiate into non-mesenchymal cells, i.e. neural cells under appropriate in vivo experimental conditions (Kopen et al., 1999; Brazelton et al., 2000; Mezey et al., 2000). This neural phenotypic plasticity allows ... More
Neural projections in planarian brain revealed by fluorescent dye tracing.
AuthorsOkamoto K, Takeuchi K, Agata K
JournalZoolog Sci
PubMed ID15930826
The planarian brain has an inverted-U shaped structure with functional regionalization. To investigate how each region in the brain connects to each other, we traced neural projections by microinjection of fluorescence dye tracers. We found that external light and olfactory/taste signals received in the head region are conveyed in the ... More
Micropatterned substrates: approach to probing intercellular communication pathways.
Intercellular signaling is critical for the normal development and physiology of the central nervous system (CNS). To study such signaling, it is vital to control where and when the cells make contact with one another. It is also important to determine whether the process used for cell localization has an ... More
Blastomeres arising from the first cleavage division have distinguishable fates in normal mouse development.
AuthorsPiotrowska K, Wianny F, Pedersen RA, Zernicka-Goetz M
JournalDevelopment
PubMed ID11585800
Two independent studies have recently suggested similar models in which the embryonic and abembryonic parts of the mouse blastocyst become separated already by the first cleavage division. However, no lineage tracing studies carried out so far on early embryos provide the support for such a hypothesis. Thus, to re-examine the ... More
G-CSF induces E-selectin ligand expression on human myeloid cells.
AuthorsDagia NM, Gadhoum SZ, Knoblauch CA, Spencer JA, Zamiri P, Lin CP, Sackstein R
JournalNat Med
PubMed ID16980970
Clinical use of G-CSF can result in vascular and inflammatory complications. To investigate the molecular basis of these effects, we analyzed the adherence of G-CSF-mobilized human peripheral blood leukocytes (ML) to inflamed (TNF-alpha-stimulated) vascular endothelium. Studies using parallel plate assays under physiologic flow conditions and intravital microscopy in a mouse ... More
Topological precision in the thalamic projection to neonatal mouse barrel cortex.
AuthorsAgmon A, Yang LT, Jones EG, O'Dowd DK
JournalJ Neurosci
PubMed ID7823163
Somatosensory thalamus and cortex in rodents contain topological representations of the facial whisker pad. The thalamic representation of a single whisker ("barreloid") is presumed to project exclusively to the cortical representation ("barrel") of the same whisker; however, it was not known when this correspondence is established during early development, nor ... More
Fluorescent labeling of blood cells for evaluation of retinal and choroidal circulation.
AuthorsKhoobehi B, Peyman GA
JournalOphthalmic Surg Lasers
PubMed ID10037209
BACKGROUND AND OBJECTIVE: To develop a method of staining and tracking cells in vivo with a scanning laser ophthalmoscope (SLO) to evaluate the retinal and choroidal circulations. MATERIALS AND METHODS: Twelve pigmented male rats were used. Staining of leukocytes. Two commercially available nucleic acid stains SYTO 16 and SYTO 59 ... More
Locally Trapping the C-C Chemokine Receptor Type 7 by Gene Delivery Nanoparticle Inhibits Lymphatic Metastasis Prior to Tumor Resection.
Authors
JournalSmall
PubMed ID30690891
Overexpression of Down syndrome cell adhesion molecule impairs precise synaptic targeting.
Authors
JournalNat Neurosci
PubMed ID23666178
NFL-lipid nanocapsules for brain neural stem cell targeting in vitro and in vivo.
AuthorsCarradori D, Saulnier P, Préat V, des Rieux A, Eyer J
JournalJ Control Release
PubMed ID27503706
'The replacement of injured neurons by the selective stimulation of neural stem cells in situ represents a potential therapeutic strategy for the treatment of neurodegenerative diseases. The peptide NFL-TBS.40-63 showed specific interactions towards neural stem cells of the subventricular zone. The aim of our work was to produce a NFL-based ... More
Reversible Shielding and Immobilization of Liposomes and Viral Vectors by Tailored Antibody-Ligand Interactions.
Authors
JournalSmall
PubMed ID34859962
Phenotypic Landscape of Schizophrenia-Associated Genes Defines Candidates and Their Shared Functions.
Authors
JournalCell
PubMed ID30929901
Micromotor-enabled active drug delivery for in vivo treatment of stomach infection.
Authors
JournalNat Commun
PubMed ID28814725
ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells.
Authors
JournalNat Commun
PubMed ID32848136
Artificial Mini Dendritic Cells Boost T Cell-Based Immunotherapy for Ovarian Cancer.
Authors
JournalAdv Sci (Weinh)
PubMed ID32274314
Imaging vesicle formation dynamics supports the flexible model of clathrin-mediated endocytosis.
Authors
JournalNat Commun
PubMed ID35365614
Exosomes Derived From Natural Killer Cells Exert Therapeutic Effect in Melanoma.
Authors
JournalTheranostics
PubMed ID28819459
DNA mechanotechnology reveals that integrin receptors apply pN forces in podosomes on fluid substrates.
Authors
JournalNat Commun
PubMed ID31628308
Quantification of the asymmetric migration of the lipophilic dyes, DiO and DiD, in homotypic co-cultures of chondrosarcoma SW-1353 cells.
AuthorsLehmann TP, Juzwa W, Filipiak K, Sujka-Kordowska P, Zabel M, Glowacki J, Glowacki M, Jagodzinski PP
JournalMol Med Rep
PubMed ID27748852
DiO and DiD are lipophilic cell labelling dyes used in the staining of cells in vivo and in vitro. The aim of the present study was to quantify the asymmetrical distribution of dyes in co-cultured cells and to measure the intercellular transfer of DiO and DiD. DiO and DiD were applied separately ... More
Heterologous and cross-species tropism of cancer-derived extracellular vesicles.
AuthorsGarofalo M, Villa A, Crescenti D, Marzagalli M, Kuryk L, Limonta P, Mazzaferro V, Ciana P
JournalTheranostics
PubMed ID31534511
Extracellular vesicles (EVs) are naturally occurring cargo delivery vesicles that have recently received considerable attention for their roles in intercellular communication in many physiological and pathological processes, including tumourigenesis. EVs generated by different tissues demonstrated specific homing: in particular, cancer-derived EVs showed a selective tropism for the tumor tissue from ... More
Podoplanin expression is a prognostic biomarker but may be dispensable for the malignancy of glioblastoma.
AuthorsEisemann T, Costa B, Harter PN, Wick W, Mittelbronn M, Angel P, Peterziel H
JournalNeuro Oncol
PubMed ID30418623
Treatment options of glioblastoma, the most aggressive primary brain tumor with frequent relapses and high mortality, are still very limited, urgently calling for novel therapeutic targets. Expression of the glycoprotein podoplanin correlates with poor prognosis in various cancer entities, including glioblastoma. Furthermore, podoplanin has been associated with tumor cell migration ... More