Dynamic behavior of cells within neurospheres in expanding populations of neural precursors.
AuthorsWang TY, Sen A, Behie LA, Kallos MS,
JournalBrain Res
PubMed ID16859652
'Large-scale expansion of neural stem and progenitor cells will be essential for clinically treating the large number of patients suffering from neurodegenerative disorders such as Parkinson''s disease. Other applications of neural stem cell technology include further research in areas such as neural development or drug testing. Neural stem cells can ... More
Interaction of Actinobacillus actinomycetemcomitans outer membrane vesicles with HL60 cells does not require leukotoxin.
AuthorsDemuth DR, James D, Kowashi Y, Kato S
JournalCell Microbiol
PubMed ID12580947
'Outer membrane derived vesicles (MVs) secreted by Actinobacillus actinomycetemcomitans JP2 contain a membranolytic leukotoxin and are toxic to human HL60 cells. To determine how MVs interact with human target cells, HL60 cells were incubated with vesicles, reacted with anti-vesicle antibodies and a FITC-labelled reporter, and visualized by confocal scanning laser ... More
Use of vascular explants for ex vivo neovascularization of biomaterials.
AuthorsIurlaro M, Sanders JE, Zhu WH, Scatena M, Mitchell SB, Nicosia RF
JournalMicrovasc Res
PubMed ID12453434
'Biomaterial polymers have been proposed as scaffolds for cell assembly in vascular bioengineering. We describe here a new method for the neovascularization of polyurethane meshes from explants of rat aorta. Aortic rings embedded in collagen-permeated polyurethane meshes and cultured in medium supplemented with fetal bovine serum and vascular endothelial growth ... More
Radiation increases invasion of gene-modified mesenchymal stem cells into tumors.
AuthorsZielske SP, Livant DL, Lawrence TS,
JournalInt J Radiat Oncol Biol Phys
PubMed ID18849123
Mesenchymal stem cells (MSCs) are multipotent cells in the bone marrow that have been found to migrate to tumors, suggesting a potential use for cancer gene therapy. MSCs migrate to sites of tissue damage, including normal tissues damaged by radiation. In this study, we investigated the effect of tumor radiotherapy ... More
Suitability of various membrane lipophilic probes for the detection of trogocytosis by flow cytometry.
AuthorsDaubeuf S, Bordier C, Hudrisier D, Joly E,
JournalCytometry A
PubMed ID19051238
Trogocytosis is a recently discovered phenomenon whereby lymphocytes capture fragments of the plasma membrane from antigen presenting cells (APCs). Using APCs labeled with widely used fluorescent lipophilic probes, we previously described a trogocytosis analysis protocol (TRAP) useful to understand the mechanisms and biological consequences of this process and to identify ... More
Agonist-induced PIP(2) hydrolysis inhibits cortical actin dynamics: regulation at a global but not at a micrometer scale.
Authorsvan Rheenen J, Jalink K
JournalMol Biol Cell
PubMed ID12221130
Phosphatidylinositol 4, 5-bisphosphate (PIP(2)) at the inner leaflet of the plasma membrane has been proposed to locally regulate the actin cytoskeleton. Indeed, recent studies that use GFP-tagged pleckstrin homology domains (GFP-PH) as fluorescent PIP(2) sensors suggest that this lipid is enriched in membrane microdomains. Here we report that this concept ... More
Fusion pore expansion is a slow, discontinuous, and Ca2+-dependent process regulating secretion from alveolar type II cells.
AuthorsHaller T, Dietl P, Pfaller K, Frick M, Mair N, Paulmichl M, Hess MW, Furst J, Maly K
JournalJ Cell Biol
PubMed ID11604423
In alveolar type II cells, the release of surfactant is considerably delayed after the formation of exocytotic fusion pores, suggesting that content dispersal may be limited by fusion pore diameter and subject to regulation at a postfusion level. To address this issue, we used confocal FRAP and N-(3-triethylammoniumpropyl)-4-(4-[dibutylamino]styryl) pyridinium dibromide ... More
Auditory Selectivity for Spectral Contrast in Cortical Neurons and Behavior.
Authors
JournalJ Neurosci
PubMed ID31826944
Virucidal nano-perforator of viral membrane trapping viral RNAs in the endosome.
Authors
JournalNat Commun
PubMed ID30643128
Recreating the perivascular niche ex vivo using a microfluidic approach.
Authors
JournalBiotechnol Bioeng
PubMed ID20672286
A Potent Germline-like Human Monoclonal Antibody Targets a pH-Sensitive Epitope on H7N9 Influenza Hemagglutinin.