An antisense oligonucleotide to 1-cys peroxiredoxin causes lipid peroxidation and apoptosis in lung epithelial cells.
AuthorsPak JH, Manevich Y, Kim HS, Feinstein SI, Fisher AB
JournalJ Biol Chem
PubMed ID12372839
'1-cys peroxiredoxin (1-cysPrx), a member of the peroxiredoxin superfamily, reduces phospholipid hydroperoxides as well as organic peroxides and H(2)O(2). To determine the physiological function(s) of 1-cysPrx, we have used an antisense strategy to suppress endogenous 1-cysPrx in L2 cells, a rat lung epithelial cell line. A 25-base antisense morpholino oligonucleotide ... More
Estimation of lipid peroxidation of live cells using a fluorescent probe, diphenyl-1-pyrenylphosphine.
AuthorsTakahashi M, Shibata M, Niki E
JournalFree Radic Biol Med
PubMed ID11440828
'Diphenyl-1-pyrenylphosphine (DPPP), which reacts with lipid hydroperoxides stoichiometrically to yield fluorescent product DPPP oxide, was used as a fluorescent probe for lipid peroxidation in live cells. DPPP was successfully incorporated into U937 cells. Incorporation of DPPP into the cell membrane was confirmed by fluorescence microscopy. Reaction of DPPP with hydroperoxides ... More
Fluorescence probes used for detection of reactive oxygen species.
AuthorsGomes A, Fernandes E, Lima JL,
JournalJ Biochem Biophys Methods
PubMed ID16297980
'Endogenously produced pro-oxidant reactive species are essential to life, being involved in several biological functions. However, when overproduced (e.g. due to exogenous stimulation), or when the levels of antioxidants become severely depleted, these reactive species become highly harmful, causing oxidative stress through the oxidation of biomolecules, leading to cellular damage ... More
Simultaneous determination of hydroperoxides of phosphatidylcholine, cholesterol esters and triacylglycerols by column-switching high-performance liquid chromatography with a post-column detection system.
AuthorsAkasaka K, Ohrui H, Meguro H
JournalJ Chromatogr
PubMed ID8150864
'A method for the simultaneous determination of hydroperoxides of phosphatidylcholines (PC), triacylglycerols (TG) and cholesterol esters (CE) has been developed. A sample was separated into a combined TG and CE hydroperoxides fraction and a PC hydroperoxides fraction on a short silica column. The fractions were introduced into an ODS column ... More
A novel fluorescent probe diphenyl-1-pyrenylphosphine to follow lipid peroxidation in cell membranes.
AuthorsOkimotoa Y, Watanabea A, Nikia E, Yamashitab T, Noguchia N
JournalFEBS Lett
PubMed ID10838073
'Diphenyl-1-pyrenylphosphine (DPPP) was tested whether it could be used as a fluorescent probe to monitor lipid peroxidation in cell membranes. DPPP reacted with organic hydroperoxides and hydrogen peroxide stoichiometrically to give DPPP oxide (DPPP = O). DPPP incorporated into phosphatidylcholine liposomal membranes and polymorphonuclear leukocytes (PMNs) reacted with methyl linoleate ... More
High-performance liquid chromatography and post-column derivatization with diphenyl-1-pyrenylphosphine for fluorimetric determination of triacylglycerol hydroperoxides.
AuthorsAkasaka K, Ijichi S, Watanabe K, Ohrui H, Meguro H
JournalJ Chromatogr
PubMed ID1400836
'Triacylglycerol monohydroperoxides (TG-mHPO) were selectively detected at the picomole levels after post-column reaction with diphenyl-1-pyrenylphosphine (DPPP). TG-mHPO were separated on two types of reserved-phase columns, an ODS column and a phenylated silica gel column, which were useful for determining TG-mHPO at their molecular species levels and their class levels, respectively. ... More
Determination of neutral lipid hydroperoxides by size exclusion HPLC with fluorometric detection. Application to fish oil enriched mayonnaises during storage.
AuthorsHartvigsen K, Hansen LF, Lund P, Bukhave K, Hølmer G
JournalJ Agric Food Chem
PubMed ID11141256
'A fast (12 min) high-performance size exclusion chromatography (HPSEC) method for the separation of neutral lipid class hydroperoxides in the oil phases from fish oil enriched mayonnaises was developed. Detection and quantification were performed using the postcolumn fluorometric (FL) diphenyl-1-pyrenylphosphine oxidation principle. The reproducibilities judged by intra- and inter-assay variations ... More
Development of phosphine reagents for the high-performance liquid chromatographic-fluorometric determination of lipid hydroperoxides.
AuthorsAkasaka K, Ohrui H
JournalJ Chromatogr A
PubMed ID10905700
'Phosphine reagents were designed and synthesized as a new type of fluorescent reagents for the determination of lipid hydroperoxides in foodstuff and biological materials. All phosphine reagents prepared had no fluorescence but their oxides, which were produced by the reaction of the phosphines with hydroperoxides, had strong fluorescence. Among the ... More
Determination of triacylglycerol and cholesterol ester hydroperoxides in human plasma by high-performance liquid chromatography with fluorometric postcolumn detection.
AuthorsAkasaka K, Ohrui H, Meguro H, Tamura M
JournalJ Chromatogr
PubMed ID8408385
'Cholesterol ester (ChE) and triacylglycerol (TG) hydroperoxides in human plasma were determined by high-performance liquid chromatography with postcolumn detection with diphenyl-1-pyrenylphosphine. Human plasma was extracted once with n-hexane. 2,6-Di-tert.-butyl-4-methylphenol and N-stearylcinnamide were added to human plasma before extraction as an antioxidation agent and an internal standard, respectively. The detection limits ... More
Determination of hydroperoxides with fluorometric reagent diphenyl-1-pyrenylphosphine.
AuthorsMeguro H, Akasaka K, Ohrui H
JournalMethods Enzymol
PubMed ID2233291
Thin-layer chromatography blotting for the fluorescence detection of phospholipid hydroperoxides and cholesteryl ester hydroperoxides.
AuthorsTerao J, Miyoshi M, Miyamoto S
JournalJ Chromatogr B Biomed Sci Appl
PubMed ID11767313
A blotting technique was developed to specifically detect lipid hydroperoxides in thin-layer chromatography. Phosphatidylcholine hydroperoxides and cholesteryl linoleate hydroperoxides ranging from 0.1 to 0.5 nmol, which were prepared by reaction with soybean lipoxygenase, were visualized as fluorescent spots on the blotted membrane by immersing the plate into a blotting solvent ... More
Detection of cholesteryl ester hydroperoxide isomers using gas chromatography-mass spectrometry combined with thin-layer chromatography blotting.
AuthorsKawai Y, Miyoshi M, Moon JH, Terao J,
JournalAnal Biochem
PubMed ID17097596
Oxidative modification of low-density lipoprotein (LDL) has been implicated in the pathogenesis of atherosclerosis. During the oxidation of LDL, cholesteryl esters, the major lipid components in LDL, are oxidized to cholesteryl ester hydroperoxides (CEOOH). The isomers of CEOOH may reflect the reactive species that initiate the peroxidation reaction. In the ... More
Nrf2 signaling, a mechanism for cellular stress resistance in long-lived mice.
AuthorsLeiser SF, Miller RA,
JournalMol Cell Biol
PubMed ID19933842
Transcriptional regulation of the antioxidant response element (ARE) by Nrf2 is important for the cellular adaptive response to toxic insults. New data show that primary skin-derived fibroblasts from the long-lived Snell dwarf mutant mouse, previously shown to be resistant to many toxic stresses, have elevated levels of Nrf2 and of ... More
Determination of hydroperoxides and structures by high-performance liquid chromatography with post-column detection with diphenyl-1-pyrenylphosphine.
AuthorsOhshima T, Hopia A, German JB, Frankel EN
JournalLipids
PubMed ID8898309
A high-performance liquid chromatographic method, using post-column detection with diphenyl-1-pyrenylphosphine (DPPP), was developed for the quantitative and qualitative determination of isomeric lipid hydroperoxides (OOH). The OOH eluted from a normal-phase column were passed through a photodiode array detector and then mixed with DPPP solution in a reaction coil heated at ... More
Automatic determination of hydroperoxides of phosphatidylcholine and phosphatidylethanolamine in human plasma.
AuthorsAkasaka K, Ohata A, Ohrui H, Meguro H
JournalJ Chromatogr B Biomed Appl
PubMed ID7795799
An automatic method for the determination of hydroperoxides of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) is reported. Sample plasma was deproteinized with a fourfold volume of methanol. After centrifugation, the supernatant was injected directly into an HPLC system without further treatment. The hydroperoxides of PC and PE were concentrated and washed ... More
Oxidation of lipids in low density lipoprotein particles.
AuthorsNoguchi N, Numano R, Kaneda H, Niki E
JournalFree Radic Res
PubMed ID9733021
This study was undertaken to understand further the mechanisms and dynamics of the oxidation of lipids in low density lipoprotein (LDL) particles, aiming specifically at elucidating the material balance between oxygen uptake and products found and also the relative susceptibilities to oxidation of cholesteryl ester in the core and phosphatidylcholine ... More
Increased level of oxidative stress in genomically unstable cell clones.
AuthorsDahle J, Kvam E
JournalJ Photochem Photobiol B
PubMed ID15043843
Recently, we reported that ultraviolet radiation induces delayed mutations in mammalian cells. At the same level of cell death the oxidative component of sunlight (ultraviolet A radiation) was as potent in inducing this kind of genomic instability as ultraviolet B radiation. Ultraviolet B radiation predominantly harms cells by direct damage ... More
Fluorescence imaging of lipid peroxidation in isolated rat lungs during nonhypoxic lung ischemia.
AuthorsMatot I, Manevich Y, Al-Mehdi AB, Song C, Fisher AB
JournalFree Radic Biol Med
PubMed ID12633755
We have shown previously that ischemia results in reactive oxygen species production by lung endothelium that occurs within 3-5 s after flow cessation and is followed by lipid peroxidation at 15-30 min as determined by assay of thiobarbituric acid-reactive substances, conjugated dienes, and protein carbonyls in lung homogenate. The present ... More
Regulation of ionizing radiation-induced apoptosis by mitochondrial NADP+-dependent isocitrate dehydrogenase.
AuthorsLee JH, Kim SY, Kil IS, Park JW
JournalJ Biol Chem
PubMed ID17350954
Ionizing radiation induces the production of reactive oxygen species, which play an important causative role in apoptotic cell death. By supplying NADPH for antioxidant systems, we recently demonstrated that the control of mitochondrial redox balance and the cellular defense against oxidative damage are some of the primary functions of mitochondrial ... More
1-Cys peroxiredoxin overexpression protects cells against phospholipid peroxidation-mediated membrane damage.
1-Cys peroxiredoxin (1-cysPrx) is a novel antioxidant enzyme able to reduce phospholipid hydroperoxides in vitro by using glutathione as a reductant. This enzyme is widely expressed and is enriched in lungs. A fusion protein of green fluorescent protein with 1-cysPrx was stably expressed in a lung-derived cell line (NCI-H441) lacking ... More