Differing effects of copper,zinc superoxide dismutase overexpression on neurotoxicity elicited by nitric oxide, reactive oxygen species, and excitotoxins.
AuthorsYing W,Anderson CM,Chen Y,Stein BA,Fahlman CS,Copin JC,Chan PH,Swanson RA
JournalJournal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
PubMed ID10698074
The effects of nitric oxide on the oxidations of l-dopa and dopamine mediated by tyrosinase and peroxidase.
AuthorsNappi AJ, Vass E
JournalJ Biol Chem
PubMed ID11136730
'The effects of nitric oxide (NO) on both tyrosinase/O(2)- and horseradish peroxidase/H(2)O(2)-mediated oxidations of dopamine and its o-dihydric phenol precursor l-dopa were compared with autoxidative processes and quantitatively assessed by oxidative and reductive electrochemical detection systems. In peroxidase/H(2)O(2)/NO-catalyzed reactions, significantly more substrate was oxidized than in the corresponding control incubations ... More
Unique oxidative mechanisms for the reactive nitrogen oxide species, nitroxyl anion.
AuthorsMiranda KM, Espey MG, Yamada K, Krishna M, Ludwick N, Kim S, Jourd'heuil D, Grisham MB, Feelisch M, Fukuto JM, Wink DA
JournalJ Biol Chem
PubMed ID11042174
'The nitroxyl anion (NO-) is a highly reactive molecule that may be involved in pathophysiological actions associated with increased formation of reactive nitrogen oxide species. Angeli''s salt (Na2N2O3; AS) is a NO- donor that has been shown to exert marked cytotoxicity. However, its decomposition intermediates have not been well characterized. ... More
Nitric oxide reversibly inhibits the migration of cultured vascular smooth muscle cells.
AuthorsSarkar R, Meinberg EG, Stanley JC, Gordon D, Webb RC
JournalCirc Res
PubMed ID8575065
'Augmentation of nitric oxide (NO) production in vivo decreases lesions in a variety of models of arterial injury, and inhibition of NO synthase exacerbates experimental intimal lesions. Both vascular smooth muscle cell (VSMC) proliferation and migration contribute to lesion formation. Although NO inhibits VSMC proliferation, its effects on VSMC migration ... More
15-Lipoxygenase catalytically consumes nitric oxide and impairs activation of guanylate cyclase.
AuthorsO'Donnell VB, Taylor KB, Parthasarathy S, Kühn H, Koesling D, Friebe A, Bloodsworth A, Darley-Usmar VM, Freeman BA
JournalJ Biol Chem
PubMed ID10400618
'Analysis of purified soybean and rabbit reticulocyte 15-lipoxygenase (15-LOX) and PA317 cells transfected with human 15-LOX revealed a rapid rate of linoleate-dependent nitric oxide (.NO) uptake that coincided with reversible inhibition of product ((13S)-hydroperoxyoctadecadienoic acid, or (13S)-HPODE) formation. No reaction of .NO (up to 2 microM) with either native (Ered) ... More
Differential sensitivity of guanylyl cyclase and mitochondrial respiration to nitric oxide measured using clamped concentrations.
AuthorsBellamy TC, Griffiths C, Garthwaite J
JournalJ Biol Chem
PubMed ID12080082
'Nitric oxide (NO) signal transduction may involve at least two targets: the guanylyl cyclase-coupled NO receptor (NO(GC)R), which catalyzes cGMP formation, and cytochrome c oxidase, which is responsible for mitochondrial O(2) consumption and which is inhibited by NO in competition with O(2). Current evidence indicates that the two targets may ... More
Exogenous nitric oxide regulates IFN-gamma plus lipopolysaccharide-induced nitric oxide synthase expression in mouse macrophages.
AuthorsSheffler LA, Wink DA, Melillo G, Cox GW
JournalJ Immunol
PubMed ID7541825
'This study was performed to determine the effects of nitric oxide (NO) on the expression of inducible NO synthase (iNOS) in mouse macrophages. We used the NO donor diethylamine dinitric oxide (DEA/NO) and the mouse macrophage cell line ANA-1 in these experiments. ANA-1 macrophages did not express iNOS mRNA either ... More
A novel reaction mechanism for the formation of S-nitrosothiol in vivo.
AuthorsGow AJ, Buerk DG, Ischiropoulos H
JournalJ Biol Chem
PubMed ID9006926
'The objective of this study was to investigate the mechanism of S-nitrosothiol formation under physiological conditions. A mechanism is proposed by which nitric oxide (.NO) reacts directly with reduced thiol to produce a radical intermediate, R-S-N.-O-H. This intermediate reduces an electron acceptor to produce S-nitrosothiol. Under aerobic conditions O2 acts ... More
Reaction of nitric oxide with the free sulfhydryl group of human serum albumin yields a sulfenic acid and nitrous oxide.
'Nitric oxide (NO) generated by diethylamine nonoate (DEA/NO), an NO donor, readily oxidized the free sulfhydryl group of human serum albumin (HSA) as well as the sulfhydryl groups of reduced glutathione (GSH) and dithiothreitol (DTT) at pH 7.4 and 37 degrees C. Under anaerobic conditions, the major products of the ... More
Superoxide modulates the oxidation and nitrosation of thiols by nitric oxide-derived reactive intermediates. Chemical aspects involved in the balance between oxidative and nitrosative stress.
AuthorsWink DA, Cook JA, Kim SY, Vodovotz Y, Pacelli R, Krishna MC, Russo A, Mitchell JB, Jourd'heuil D, Miles AM, Grisham MB
JournalJ Biol Chem
PubMed ID9111012
'Thiol-containing proteins are key to numerous cellular processes, and their functions can be modified by thiol nitrosation or oxidation. Nitrosation reactions are quenched by O-2, while the oxidation chemistry mediated by peroxynitrite is quenched by excess flux of either NO or O-2. A solution of glutathione (GSH), a model thiol-containing ... More
Kinetics and mechanism of tetrahydrobiopterin-induced oxidation of nitric oxide.
AuthorsMayer B, Klatt P, Werner ER, Schmidt K
JournalJ Biol Chem
PubMed ID7529763
'A Clark-type nitric oxide-sensitive electrode was used for electrochemical determination of NO oxidation kinetics. Reaction with molecular oxygen followed second-order rate law with respect to NO with an overall rate constant of 9.2 +/- 0.33 x 10(6) M-2 s-1. Tetrahydrobiopterin, an essential cofactor of NO synthases, was found to induce ... More
Measuring nitric oxide in single neurons by capillary electrophoresis with laser-induced fluorescence: use of ascorbate oxidase in diaminofluorescein measurements.
AuthorsKim WS, Ye X, Rubakhin SS, Sweedler JV
JournalAnal Chem
PubMed ID16536421
'As a family of novel fluorescent indicators for nitric oxide (NO), the diaminofluoresceins (DAFs) have allowed real-time measurement of neuronal NO, an important gaseous neurotransmitter. However, the measurement of NO by the most commonly used NO sensor, 4,5-diaminofluorescein (DAF-2), is altered by two processes: the interaction of DAF-2 with intracellular ... More
N-Methyl-D-aspartate receptor stimulation activates tyrosinase and promotes melanin synthesis in the ink gland of the cuttlefish Sepia officinalis through the nitric Oxide/cGMP signal transduction pathway. A novel possible role for glutamate as physiologic activator of melanogenesis.
AuthorsPalumbo A, Poli A, Di Cosmo A, d'Ischia M
JournalJ Biol Chem
PubMed ID10748137
'The tyrosinase-catalyzed conversion of l-tyrosine to melanin represents the most distinctive biochemical pathway in the ink gland of the cuttlefish Sepia officinalis; however, the molecular mechanisms underlying its activation have remained so far largely uncharted. In this paper we demonstrate for the first time that l-glutamate can stimulate tyrosinase activity ... More
AuthorsTrotti D, Rossi D, Gjesdal O, Levy LM, Racagni G, Danbolt NC, Volterra A
JournalJ Biol Chem
PubMed ID8626378
'The reuptake of glutamate in neurons and astrocytes terminates excitatory signals and prevents the persistence of excitotoxic levels of glutamate in the synaptic cleft. This process is inhibited by oxygen radicals and hydrogen peroxide (H2O2). Here we show that another biological oxidant, peroxynitrite (ONOO-), formed by combination of superoxide (O2-) ... More
'To examine the role of nitric oxide (.NO) in vascular endothelial cell injury, cultured porcine pulmonary artery endothelial cells (PAEC) were treated with H2O2 (100-500 microM) for 30 min in the presence or absence of the .NO donors (+/-)S-nitroso-N-acetylpenicillamine (SNAP) or diethylamine nitric oxide (DEANO). H2O2 caused dose-dependent PAEC cytotoxicity ... More
Ultraviolet B radiation acts through the nitric oxide and cGMP signal transduction pathway to stimulate melanogenesis in human melanocytes.
AuthorsRoméro-Graillet C, Aberdam E, Biagoli N, Massabni W, Ortonne JP, Ballotti R
JournalJ Biol Chem
PubMed ID8910416
'Ultraviolet B (UVB) radiation is the main physiological stimulus for human skin pigmentation; however, the molecular mechanisms underlying this process are still unclear. Recently, nitric oxide (NO) and cGMP have been involved in mediation of skin erythema induced by UVB. Therefore, we investigated the role of NO and cGMP in ... More
Peroxynitrite-induced accumulation of cyclic GMP in endothelial cells and stimulation of purified soluble guanylyl cyclase. Dependence on glutathione and possible role of S-nitrosation.
AuthorsMayer B, Schrammel A, Klatt P, Koesling D, Schmidt K
JournalJ Biol Chem
PubMed ID7615539
'Peroxynitrite (ONOO-) is widely recognized as mediator of NO toxicity, but recent studies have indicated that this compound may also have physiological activity and induce vascular relaxation as well as inhibition of platelet aggregation. We found that ONOO- induced a pronounced increase in endothelial cyclic GMP levels, and that this ... More
Inactivation of brain tryptophan hydroxylase by nitric oxide.
AuthorsKuhn DM, Arthur RE
JournalJ Neurochem
PubMed ID8752114
Tryptophan hydroxylase, the initial and rate-limiting enzyme in the biosynthesis of the neurotransmitter serotonin, is inactivated by nitric oxide (NO) and by the NO generators sodium nitroprusside, diethylamine/NO, S-nitroso-N-acetylpenicillamine, and S-nitrosocysteine. The inactivation occurs in an oxygen-free environment and is enhanced by dithiothreitol and ascorbic acid. Protection against the effect ... More
A cell-based cGMP assay useful for ultra-high-throughput screening and identification of modulators of the nitric oxide/cGMP pathway.
We have established a rapid, homogeneous, cell-based, and highly sensitive assay for guanosine 3'-5'-cyclic monophosphate (cGMP) that is suitable for fully automated ultra-high-throughput screening. In this assay system, cGMP production is monitored in living cells via Ca2+ influx through the olfactory cyclic nucleotide-gated cation channel CNGA2, acting as the intracellular ... More
Heme and the endothelium. Effects of nitric oxide on catalytic iron and heme degradation by heme oxygenase.
AuthorsJuckett M, Zheng Y, Yuan H, Pastor T, Antholine W, Weber M, Vercellotti G
JournalJ Biol Chem
PubMed ID9722574
We studied the effects of nitric oxide (NO) on the control of excess cellular heme and release of catalytically active iron. Endothelial cells (ECs) exposed to hemin followed by a NO donor have a ferritin content that is 16% that of cells exposed to hemin alone. Hemin-treated ECs experience a ... More
Functions of conserved cysteines of soluble guanylyl cyclase.
AuthorsFriebe A, Wedel B, Harteneck C, Foerster J, Schultz G, Koesling D
JournalBiochemistry
PubMed ID9063867
Soluble guanylyl cyclase (sGC), a heme-containing heterodimeric enzyme, is stimulated by NO and catalyzes the formation of the intracellular signaling molecule cGMP. Cysteine residues of sGC have been considered to be important as they were thought to play a significant role in the regulation of the enzyme. The aim of ... More
A variant of the alpha 2 subunit of soluble guanylyl cyclase contains an insert homologous to a region within adenylyl cyclases and functions as a dominant negative protein.
AuthorsBehrends S, Harteneck C, Schultz G, Koesling D
JournalJ Biol Chem
PubMed ID7673142
A variant of the alpha 2 subunit of soluble guanylyl cyclase (alpha 2i) containing 31 additional amino acids was identified in a number of cell lines and tissues. The in-frame sequence of the insert was within the proposed catalytic domain of guanylyl cyclases and was homologous to a region within ... More
Cu,Zn-superoxide dismutase-dependent apoptosis induced by nitric oxide in neuronal cells.
AuthorsCiriolo MR, De Martino A, Lafavia E, Rossi L, Carrì MT, Rotilio G
JournalJ Biol Chem
PubMed ID10671549
Nitric oxide (NO) challenge to human neuroblastoma cells (SH-SY5Y) ultimately results in apoptosis. Tumor suppressor protein p53 and cell cycle inhibitor p21 accumulate as an early sign of S-nitrosoglutathione-mediated toxicity. Cytochrome c release from mitochondria and caspase 3 activation also occurred. Cells transfected with either wild type (WT) or mutant ... More
Nitric oxide protects against alkyl peroxide-mediated cytotoxicity: further insights into the role nitric oxide plays in oxidative stress.
AuthorsWink DA, Cook JA, Krishna MC, Hanbauer I, DeGraff W, Gamson J, Mitchell JB
JournalArch Biochem Biophys
PubMed ID7786021
Endogenously formed nitric oxide (NO) possesses diverse properties such as regulating physiological functions, exerting specific toxic effects, and protecting against various toxic substances. Recent studies suggest that in the presence of reactive oxygen species, NO can serve as an antioxidant. We show here that NO delivered from the NO donor ... More
A spectrophotometric method for the direct detection and quantitation of nitric oxide, nitrite, and nitrate in cell culture media.
AuthorsRidnour LA, Sim JE, Hayward MA, Wink DA, Martin SM, Buettner GR, Spitz DR
JournalAnal Biochem
PubMed ID10870839
A method for the spectrophotometric determination of nitric oxide, nitrite, and nitrate in tissue culture media is presented. The method is based on the nitric oxide-mediated nitrosative modification of sulfanilic acid that reacts with N-(1-naphthyl)ethylenediamine dihydrochloride forming an orange-colored product absorbing at 496 nm. Nitric oxide levels were determined in ... More
Differential modulation of prostaglandin H synthase-2 by nitric oxide-related species in intact cells.
Nitrogen monoxide (NO) has been reported to both activate and inhibit prostaglandin (PG) biosynthesis. This apparent paradox might be explained by the production/action of distinct NO-related species formed as a result of the prevailing redox states of different cellular systems. As such, the effect of NO donors with different redox ... More
Regulation of presynaptic phosphatidylinositol 4,5-biphosphate by neuronal activity.
AuthorsMicheva KD, Holz RW, Smith SJ
JournalJ Cell Biol
PubMed ID11470824
Phosphatidylinositol 4,5-biphosphate (PIP2) has been implicated in a variety of cellular processes, including synaptic vesicle recycling. However, little is known about the spatial distribution of this phospholipid in neurons and its dynamics. In this study, we have focused on these questions by transiently expressing the phospholipase C (PLC)-delta1 pleckstrin homology ... More
The oxidative and nitrosative chemistry of the nitric oxide/superoxide reaction in the presence of bicarbonate.
AuthorsJourd'heuil D, Miranda KM, Kim SM, Espey MG, Vodovotz Y, Laroux S, Mai CT, Miles AM, Grisham MB, Wink DA
JournalArch Biochem Biophys
PubMed ID10222043
The primary product of the interaction between nitric oxide (NO) and superoxide () is peroxynitrite (ONOO-), which is capable of either oxidizing or nitrating various biological substrates. However, it has been shown that excess NO or can further react with ONOO- to form species which mediate nitrosation. Subsequently, the controlled ... More
Nitric oxide/nucleophile complexes inhibit the in vitro proliferation of A375 melanoma cells via nitric oxide release.
AuthorsMaragos CM, Wang JM, Hrabie JA, Oppenheim JJ, Keefer LK
JournalCancer Res
PubMed ID8425188
Cell-mediated antitumor effects have, in part, been attributed to the production of NO. Compounds which generate NO might, therefore, be useful in attenuating the growth of tumor cells. Six nitric oxide/nucleophile adducts that release NO spontaneously in solution were tested for their effectiveness in inhibiting DNA synthesis in A375 human ... More
Nitric oxide inhibition of endothelial cell mitogenesis and proliferation.
AuthorsSarkar R, Webb RC, Stanley JC
JournalSurgery
PubMed ID7638744
BACKGROUND. Endothelial cell (EC) proliferation is essential in vascular repair after injury to the vessel wall. Impaired EC proliferation may be an important factor contributing to vessel wall disease. Nitric oxide (NO) inhibits proliferation of many cells, including smooth muscle cells (SMC). We tested the hypothesis that NO inhibits EC ... More
Nitric oxide attenuates vascular smooth muscle cell activation by interferon-gamma. The role of constitutive NF-kappa B activity.
AuthorsShin WS, Hong YH, Peng HB, De Caterina R, Libby P, Liao JK
JournalJ Biol Chem
PubMed ID8626684
Atherogenesis involves cellular immune responses and altered vascular smooth muscle cell (SMC) function. Cytokines such as interleukin (IL)-1 alpha and interferon-gamma (IFN-gamma) may contribute to this process by activating SMC. To determine whether the anti-atherogenic mediator, nitric oxide (.NO), can modulate cytokine-induced SMC activation, we investigated the effects of various ... More
Nitric oxide trapping of tyrosyl radicals generated during prostaglandin endoperoxide synthase turnover. Detection of the radical derivative of tyrosine 385.
Tyrosyl radicals have been detected during turnover of prostaglandin endoperoxide H synthase (PGHS), and they are speculated to participate in cyclooxygenase catalysis. Spectroscopic approaches to elucidate the identity of the radicals have not been definitive, so we have attempted to trap the radical(s) with nitric oxide (NO). NO quenched the ... More
Inotropic effects of glyceryl trinitrate and spontaneous NO donors in the dog heart.
AuthorsPreckel B, Kojda G, Schlack W, Ebel D, Kottenberg K, Noack E, Thämer V
JournalCirculation
PubMed ID9355909
BACKGROUND: In vitro, NO has a biphasic effect on myocardial inotropy. To determine the inotropic effect of NO in vivo, we investigated the activity of glyceryl trinitrate (GTN) and the NO donors S-nitroso-N-acetyl-D,L-penicillamine (SNAP) and sodium-(2)-1-(N,N-diethyl-amino)-diazen-1-ium-1,2-diolat+ ++ (DEA/NO) in dogs. METHODS AND RESULTS: Eight anesthetized open-chest dogs were instrumented for ... More
Nuclear factor-kappa B and mitogen-activated protein kinases mediate nitric oxide-enhanced transcriptional expression of interferon-beta.
AuthorsJacobs AT, Ignarro LJ
JournalJ Biol Chem
PubMed ID12500976
Mitogen-activated protein (MAP) kinase and nuclear factor-kappaB (NF-kappaB) activation are critical for initiating the transcriptional expression of cytokines, cell adhesion molecules, and other factors in the macrophage immune response. Nitric oxide (NO), an endogenous free radical, is a product of macrophages that mediates inflammatory and cytotoxic processes in the immune ... More
Inhibition of cytochromes P450 by nitric oxide and a nitric oxide-releasing agent.
AuthorsWink DA, Osawa Y, Darbyshire JF, Jones CR, Eshenaur SC, Nims RW
JournalArch Biochem Biophys
PubMed ID8424643
The effect of nitric oxide (NO) on cytochrome P450-mediated benzyloxyresorufin and ethoxyresorufin O-dealkylase activity of rat hepatic postmitochondrial (S-9) or microsomal subfractions, or purified rat liver CYP2B1, was examined. Two distinct inhibitory phases were observed regardless of whether the NO was added prior to initiation of the reactions with NADPH ... More
Increased cytotoxicity of 3-morpholinosydnonimine to HepG2 cells in the presence of superoxide dismutase. Role of hydrogen peroxide and iron.
AuthorsGergel D, Misík V, Ondrias K, Cederbaum AI
JournalJ Biol Chem
PubMed ID7673115
3-Morpholinosydnonimine (SIN-1) is widely used to generate nitric oxide (NO(x).) and superoxide radical (O2-.). The effect of SOD on the toxicity of SIN-1 is complex, depending on what is the ultimate species responsible for toxicity. SIN-1 (< 1 mM) was only slightly toxic to HepG2 cells. Copper, zinc superoxide dismutase ... More
Nitric oxide modification of rat brain neurogranin affects its phosphorylation by protein kinase C and affinity for calmodulin.
AuthorsSheu FS, Mahoney CW, Seki K, Huang KP
JournalJ Biol Chem
PubMed ID8798403
Neurogranin (Ng) is a prominent protein kinase C (PKC) substrate which binds calmodulin (CaM) in the absence of Ca2+. Rat brain Ng contains four cysteine residues that were readily oxidized by nitric oxide (NO) donors, 1,1-diethyl-2-hydroxy-2-nitrosohydrazine (DEANO) and sodium nitroprusside, and by oxidants, H2O2 and o-iodosobenzoic acid. NO oxidation of ... More
Complexes of .NO with nucleophiles as agents for the controlled biological release of nitric oxide. Vasorelaxant effects.
AuthorsMaragos CM, Morley D, Wink DA, Dunams TM, Saavedra JE, Hoffman A, Bove AA, Isaac L, Hrabie JA, Keefer LK
JournalJ Med Chem
PubMed ID1956043
Selected nucleophile/nitric oxide adducts [compounds which contain the anionic moiety, XN(O-)N = O] were studied for their ability to release nitric oxide spontaneously in aqueous solution and for possible vasoactivity. The diversity of structures chosen included those in which the nucleophile residue, X, was that of a secondary amine [Et2N, ... More
Role of cyclic GMP-dependent protein kinase in the contractile response to exogenous nitric oxide in rat cardiac myocytes.
AuthorsLayland J, Li JM, Shah AM
JournalJ Physiol
PubMed ID11956336
Nitric oxide (NO) can directly modulate cardiac contractility by accelerating relaxation and reducing diastolic tone. The intracellular mechanisms underlying these contractile effects are poorly understood. Here we investigate the role of cyclic GMP-dependent protein kinase (PKG) in the contractile response to exogenous NO in rat ventricular myocytes. Isolated ventricular myocytes ... More
Nitric oxide-induced release of acetylcholine in the nucleus accumbens: role of cyclic GMP, glutamate, and GABA.
AuthorsPrast H, Tran MH, Fischer H, Philippu A
JournalJ Neurochem
PubMed ID9648874
We have previously shown that the basal acetylcholine release in the ventral striatum is under the enhancing influence of endogenous nitric oxide (NO) and that NO donors cause pronounced increases in the acetylcholine release rate. To investigate the role of cyclic GMP, glutamate, and GABA in the NO-induced acetylcholine release, ... More
Isolation and characterization of diazoate intermediate upon nitrous acid and nitric oxide treatment of 2'-deoxycytidine.
AuthorsSuzuki T, Nakamura T, Yamada M, Ide H, Kanaori K, Tajima K, Morii T, Makino K
JournalBiochemistry
PubMed ID10353825
The intermediate produced from dCyd by HNO2 and NO treatments was isolated and characterized. When 10 mM dCyd was treated with 100 mM NaNO2 in 1.0 M acetate buffer (pH 3.7) at 37 degrees C, a previously unidentified product was formed. By spectrometric measurements, the product was identified as a ... More
Nitric oxide inhibits capacitative Ca2+ entry by suppression of mitochondrial Ca2+ handling.
AuthorsThyagarajan B, Malli R, Schmidt K, Graier WF, Groschner K
JournalBr J Pharmacol
PubMed ID12411413
1. Nitric oxide (NO) is a key modulator of cellular Ca(2+) signalling and a determinant of mitochondrial function. Here, we demonstrate that NO governs capacitative Ca(2+) entry (CCE) into HEK293 cells by impairment of mitochondrial Ca(2+) handling. 2. Authentic NO as well as the NO donors 1-[2-(carboxylato)pyrrolidin-1-yl]diazem-1-ium-1,2-diolate (ProliNO) and 2-(N,N-diethylamino)-diazenolate-2-oxide ... More
Inhibition of the catalytic activity of alcohol dehydrogenase by nitric oxide is associated with S nitrosylation and the release of zinc.
AuthorsGergel D, Cederbaum AI
JournalBiochemistry
PubMed ID8973191
Nitric oxide (NO) reacts with the sulfhydryl groups of proteins to form nitroso thiols. Alcohol dehydrogenase (ADH) plays an important role in the metabolism of ethanol. Chronic alcohol administration stimulates NO formation in the liver, and production of NO is increased in alcohol liver injury. The effect of exogenous and ... More
Distinction between nitrosating mechanisms within human cells and aqueous solution.
AuthorsEspey MG, Miranda KM, Thomas DD, Wink DA
JournalJ Biol Chem
PubMed ID11404354
The quintessential nitrosating species produced during NO autoxidation is N(2)O(3). Nitrosation of amine, thiol, and hydroxyl residues can modulate critical cell functions. The biological mechanisms that control reactivity of nitrogen oxide species formed during autoxidation of nano- to micromolar levels of NO were examined using the synthetic donor NaEt(2)NN(O)NO (DEA/NO), ... More
Characterization of nitric oxide-stimulated ADP-ribosylation of various proteins from the mouse macrophage cell line ANA-1 using sodium nitroprusside and the novel nitric oxide-donating compound diethylamine dinitric oxide.
AuthorsSheffler LA, Wink DA, Melillo G, Cox GW
JournalJ Leukoc Biol
PubMed ID7530278
We examined the ability of nitric oxide (NO) to stimulate the ADP-ribosylation of proteins from the mouse macrophage cell line ANA-1. To demonstrate a specific effect of NO, we used a novel compound named diethylamine dinitric oxide (DEA/NO; 1,1-diethyl-2-hydroxy-2-nitrosohydrazine, sodium salt; [Et2NN(O)NO]Na), which releases NO in aqueous solution at neutral ... More
Ischemia reperfusion injury in tumors: the role of oxygen radicals and nitric oxide.
AuthorsParkins CS, Dennis MF, Stratford MR, Hill SA, Chaplin DJ
JournalCancer Res
PubMed ID8521386
Oxidative stress is a key process involved in the action of several therapeutic modalities used in cancer treatment. Ischemia reperfusion insult provides a model system for investigating the processes involved in determining the sensitivity of tumor tissue to oxidative stress. We have investigated the response of the murine CaNT tumor ... More
The Fpg protein, a DNA repair enzyme, is inhibited by the biomediator nitric oxide in vitro and in vivo.
AuthorsWink DA, Laval J
JournalCarcinogenesis
PubMed ID7955043
Nitric oxide has been shown to be a mediator molecule in the regulation of many physiological functions. However, this small diatomic molecule in the presence of O2 generates reactive intermediates which modify DNA bases and inactive enzymes at high concentrations (100 microM). We report that NO generated by 1,1-diethyl-2-hydroxy-2-nitrosohydrazine (DEA/NO, ... More
A new pathway of nitric oxide/cyclic GMP signaling involving S-nitrosoglutathione.
AuthorsMayer B, Pfeiffer S, Schrammel A, Koesling D, Schmidt K, Brunner F
JournalJ Biol Chem
PubMed ID9452441
Nitric oxide (NO), a physiologically important activator of soluble guanylyl cyclase (sGC), is synthesized from L-arginine and O2 in a reaction catalyzed by NO synthases (NOS). Previous studies with purified NOS failed to detect formation of free NO, presumably due to a fast inactivation of NO by simultaneously produced superoxide ... More
Expression of Trp3 determines sensitivity of capacitative Ca2+ entry to nitric oxide and mitochondrial Ca2+ handling: evidence for a role of Trp3 as a subunit of capacitative Ca2+ entry channels.
AuthorsThyagarajan B, Poteser M, Romanin C, Kahr H, Zhu MX, Groschner K
JournalJ Biol Chem
PubMed ID11600493
The role of Trp3 in cellular regulation of Ca(2+) entry by NO was studied in human embryonic kidney (HEK) 293 cells. In vector-transfected HEK293 cells (controls), thapsigargin (TG)-induced (capacitative Ca(2+) entry (CCE)-mediated) intracellular Ca(2+) signals and Mn(2+) entry were markedly suppressed by the NO donor 2-(N,N-diethylamino)diazenolate-2-oxide sodium salt (3 microm) ... More
Mechanism of endothelial dysfunction in apolipoprotein E-deficient mice.
Authorsd'Uscio LV, Baker TA, Mantilla CB, Smith L, Weiler D, Sieck GC, Katusic ZS
JournalArterioscler Thromb Vasc Biol
PubMed ID11397713
Endothelium-dependent relaxations mediated by NO are impaired in a mouse model of human atherosclerosis. Our objective was to characterize the mechanisms underlying endothelial dysfunction in aortas of apolipoprotein E (apoE)-deficient mice, treated for 26 to 29 weeks with a lipid-rich Western-type diet. Aortic rings from apoE-deficient mice showed impaired endothelium-dependent ... More