Measurement of nitric oxide in murine Hepatoma Hepa1c1c7 cells by reversed phase HPLC with fluorescence detection.
AuthorsGharavi N, El-Kadi AO
JournalJ Pharm Pharm Sci
PubMed ID12935442
PURPOSE: Nitric oxide (NO) is produced by various cell types in picomolar to nanomolar range and has important roles in a variety of biological functions. The aim of the present study was to investigate a sensitive and reproducible fluorometric HPLC method for measuring nitrite, one of the stable oxidation products ... More
Spectrofluorimetric determination and thin-layer chromatographic identification of selenium in foods.
AuthorsMoreno-Domínquez T, García-Moreno C, Mariné-Font A
JournalAnalyst
PubMed ID6869843
Fluorometric determination of selenium in biological material with 2,3-diaminonaphthalene.
AuthorsWatkinson JH
JournalAnal Chem
PubMed ID5900773
The synthesis and properties of 4,5-dioxovaleric acid, a possible intermediate in the biosynthesis of 5-aminolaevulinic acid, and its in vivo formation in Scenedesmus obliquus.
AuthorsDörnemann D, Senger H
JournalBiochim Biophys Acta
PubMed ID7357029
'The formation of 5-aminolaevulinic acid for chlorophyll biosynthesis in the pigment mutant C-2A'' of the unicellular green alga Scenedesmus obliquus occurs via two pathways: the first and major pathway uses glycine and succinyl-CoA as precursors and the second, the C-5 pathway, uses the intact five C-atom skeleton of either glutamate ... More
Focusing of nitric oxide mediated nitrosation and oxidative nitrosylation as a consequence of reaction with superoxide.
AuthorsEspey MG, Thomas DD, Miranda KM, Wink DA
JournalProc Natl Acad Sci U S A
PubMed ID12177414
'The impact of nitric oxide (NO) synthesis on different biological cascades can rapidly change dependent on the rate of NO formation and composition of the surrounding milieu. With this perspective, we used diaminonaphthalene (DAN) and diaminofluorescein (DAF) to examine the nitrosative chemistry derived from NO and superoxide (O2-) simultaneously generated ... More
Unique oxidative mechanisms for the reactive nitrogen oxide species, nitroxyl anion.
AuthorsMiranda KM, Espey MG, Yamada K, Krishna M, Ludwick N, Kim S, Jourd'heuil D, Grisham MB, Feelisch M, Fukuto JM, Wink DA
JournalJ Biol Chem
PubMed ID11042174
'The nitroxyl anion (NO-) is a highly reactive molecule that may be involved in pathophysiological actions associated with increased formation of reactive nitrogen oxide species. Angeli''s salt (Na2N2O3; AS) is a NO- donor that has been shown to exert marked cytotoxicity. However, its decomposition intermediates have not been well characterized. ... More
Selenium and glutathione peroxidases in blood of patients with different stages of chronic renal failure.
AuthorsZachara BA, Salak A, Koterska D, Manitius J, Wasowicz W
JournalJ Trace Elem Med Biol
PubMed ID15139391
'In patients with chronic renal failure (CRF) Se concentration in blood components is usually lower as compared with healthy controls. One of the five known forms of Se-dependent glutathione peroxidases (GSH-Px), the plasma GSH-Px, is synthesized primarily in the kidney. In CRF patients, plasma GSH-Px activity is reduced and the ... More
Effects of superoxide on nitric oxide-dependent N-nitrosation reactions.
'Recent studies have demonstrated that nitric oxide (NO) in the presence of superoxide (O2-) may mediate mutagenesis via the N-nitrosation of DNA bases followed by nitrosative deamination to yield their hydroxylated derivatives. We have found that phorbol myristate acetate (PMA)-activated extravasated rat neutrophils (PMNs) will N-nitrosate 2,3-diaminonaphthalene (DAN) to yield ... More
Neutrophil-mediated nitrosamine formation: role of nitric oxide in rats.
AuthorsGrisham MB, Ware K, Gilleland HE, Gilleland LB, Abell CL, Yamada T
JournalGastroenterology
PubMed ID1397883
'It is well known that chronic inflammation of the colon and rectum is associated with an increased risk of colorectal cancer, but the mechanisms by which inflammation promotes neoplasia remain undefined. The authors propose that inflammatory neutrophils may produce carcinogenic nitrosamines via the L-arginine-dependent formation of nitrogen oxides such as ... More
Inhibition of 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase blocks hypoxia-mediated down-regulation of endothelial nitric oxide synthase.
AuthorsLaufs U, Fata VL, Liao JK
JournalJ Biol Chem
PubMed ID9395516
'Hypoxia induces vasoconstriction, in part, by down-regulating endothelial cell nitric oxide synthase (ecNOS) expression. Previous studies indicate that 3-hydroxy-3-methylglutaryl-coenzyme A (HMG CoA) reductase inhibitors improve endothelium-dependent relaxation by increasing ecNOS activity. To determine whether HMG CoA reductase inhibitors can prevent hypoxia-mediated down-regulation of ecNOS function and expression, human endothelial cells ... More
A fluorometric assay for the measurement of nitrite in biological samples.
AuthorsMisko TP, Schilling RJ, Salvemini D, Moore WM, Currie MG
JournalAnal Biochem
PubMed ID7504409
'The increasing importance of nitric oxide synthase has been underscored by the elucidation of its role in a growing number of normal and pathophysiological processes. Therefore, techniques for detection of nitrite/nitrate, oxidation products of the enzymatic conversion of arginine to citrulline and nitric oxide, should serve as useful tools in ... More
3-Deoxyglucosone, a promoter of advanced glycation end products in fluids for peritoneal dialysis.
AuthorsLinden T, Forsbäck G, Deppisch R, Henle T, Wieslander A
JournalPerit Dial Int
PubMed ID9663893
'OBJECTIVE: The accumulation of irreversible formed advanced glycosylation end products (AGE) in the peritoneal cavity might play an important role in the development of ultrafiltration failure and peritoneal membrane destruction. 3-Deoxyglucosone (3-DG), more formally named 3-deoxy-D-erythro-hexos-2-ulose or 3-deoxy-D-erythro-hexosulos is known to be a potent cross-linker responsible for the polymerization of ... More
Inhibitory effects of nitric oxide and nitrosative stress on dopamine-beta-hydroxylase.
AuthorsZhou X, Espey MG, Chen JX, Hofseth LJ, Miranda KM, Hussain SP, Wink DA, Harris CC
JournalJ Biol Chem
PubMed ID10887204
'Dopamine-beta-hydroxylase (DbetaH) is a copper-containing enzyme that uses molecular oxygen and ascorbate to catalyze the addition of a hydroxyl group on the beta-carbon of dopamine to form norepinephrine. While norepinephrine causes vasoconstriction following reflex sympathetic stimulation, nitric oxide (NO) formation results in vasodilatation via a guanylyl cyclase-dependent mechanism. In this ... More
Calcium-dependent nitric oxide production is involved in arsenite-induced micronuclei.
AuthorsGurr JR, Liu F, Lynn S, Jan KY
JournalMutat Res
PubMed ID9729339
'Arsenic, a human carcinogen is known to induce sister-chromatid exchanges, chromosome aberrations and micronuclei (MN), but its mechanisms remain unknown. Recently, independent studies have suggested that intracellular calcium and reactive oxygen species are involved in arsenite-induced MN, and nitric oxide (NO) is involved in arsenite-induced poly(ADP-ribosylation). The aim of this ... More
Possible contribution of follicular interleukin-1beta to nitric oxide generation in human pre-ovulatory follicles.
AuthorsTao M, Kodama H, Kagabu S, Fukuda J, Murata M, Shimizu Y, Hirano H, Tanaka T
JournalHum Reprod
PubMed ID9402285
'The aim of this study was to investigate the relationships between follicular nitric oxide (NO) metabolite concentrations and several related variables, with special reference to follicular interleukin-1beta (IL-1beta). The follicular fluid from the leading and secondary follicles was collected individually from 20 women undergoing in-vitro fertilization (IVF) treatment, and the ... More
Superoxide modulates the oxidation and nitrosation of thiols by nitric oxide-derived reactive intermediates. Chemical aspects involved in the balance between oxidative and nitrosative stress.
AuthorsWink DA, Cook JA, Kim SY, Vodovotz Y, Pacelli R, Krishna MC, Russo A, Mitchell JB, Jourd'heuil D, Miles AM, Grisham MB
JournalJ Biol Chem
PubMed ID9111012
'Thiol-containing proteins are key to numerous cellular processes, and their functions can be modified by thiol nitrosation or oxidation. Nitrosation reactions are quenched by O-2, while the oxidation chemistry mediated by peroxynitrite is quenched by excess flux of either NO or O-2. A solution of glutathione (GSH), a model thiol-containing ... More
Use of colorimetric test strips for monitoring the effect of hemodialysis on salivary nitrite and uric acid in patients with end-stage renal disease: a proof of principle.
AuthorsBlicharz TM, Rissin DM, Bowden M, Hayman RB, DiCesare C, Bhatia JS, Grand-Pierre N, Siqueira WL, Helmerhorst EJ, Loscalzo J, Oppenheim FG, Walt DR,
JournalClin Chem
PubMed ID18676588
'BACKGROUND: Initial screening of potential biomarkers for monitoring dialysis was performed with saliva samples collected from patients with end-stage renal disease (ESRD). A more thorough analysis of the most promising markers identified in the initial screening was conducted with saliva samples acquired at hourly intervals throughout dialysis to monitor analyte ... More
Synthesis and cytotoxicity of substituted 2-benzylnaphth[2,3-d]imidazoles.
AuthorsGrella GE, Cabras MC, Murineddu G, Pau A, Pinna GA
JournalEur J Pharm Sci
PubMed ID14592692
'Designed as a new series of so called "bivalent ligand" containing the proposed 2-benzylnaphthimidazole-type structure, a number of 2-benzylnaphth[2,3-d]imidazoles, bearing various substituents, have been prepared by a synthetic approach involving an heterocyclization of 2,3-diaminonaphthalene 4 with appropriate imidates 3 (for 1b-i) followed by alkylation (for 1j-l) with the desired alkylating ... More
Diaminonaphthalenes and related aminocompounds: mutagenicity, CYP1A induction and interaction with the Ah receptor.
AuthorsCheung YL, Lewis DF, Ridd TI, Gray TJ, Ioannides C
JournalToxicology
PubMed ID9129166
'Using 1- and 2-aminonaphthalene as model substrates, we investigated the effect of insertion of a second amino group on mutagenicity, binding to the cytosolic Ah receptor and CYP1A inducibility, and the effects were compared to those elicited by 3,3''-diaminobenzidine and 1-naphthylethylenediamine. 1,5- and 1,8-diaminonaphthalene were effective inducers of CYP1A activity, ... More
Activation of tumor necrosis factor-alpha-converting enzyme-mediated ectodomain shedding by nitric oxide.
AuthorsZhang Z, Kolls JK, Oliver P, Good D, Schwarzenberger PO, Joshi MS, Ponthier JL, Lancaster JR
JournalJ Biol Chem
PubMed ID10747938
'Ectodomain shedding of cell surface proteins is an important process in a wide variety of physiological and developmental events. Recently, tumor necrosis factor-alpha-converting enzyme (TACE) has been found to play an essential role in the shedding of several critical surface proteins, which is evidenced by multiple developmental defects exhibited by ... More
Sources of interference in the use of 2,3-diaminonaphthalene for the fluorimetric determination of nitric oxide synthase activity in biological samples.
AuthorsFernández-Cancio M, Fernández-Vitos EM, Centelles JJ, Imperial S
JournalClin Chim Acta
PubMed ID11580928
'The use of 2,3-diaminonaphthalene (DAN) for the fluorimetric determination of nitric oxide synthase (NOS) activity in rat brain extracts has been re-examined. Two types of interference were observed, due either to components of the reaction mixture or to the enzymatic sample itself. One of the substrates (NADPH) and some cofactors ... More
High-performance liquid chromatographic-fluorescence determination of traces of selenium in biological materials.
AuthorsHawkes WC, Kutnink MA
JournalAnal Biochem
PubMed ID8921189
'An improved method for the determination of selenium in biological materials has been developed. This work both extends and validates the procedure of Vézina and Bleau (J. Chromatog. 426, 385-391, 1988) which is based on high-performance liquid chromatographic determination of the fluorophore formed by reaction of Se(IV) with 2,3-diaminonaphthalene. The ... More
Elimination of matrix-based interferences to a fluorescent nitrite/nitrate assay by a simple filtration procedure.
'Pathophysiological levels of oxygen radical metabolites have been studied as indicators of trauma caused by burn insult. The 2, 3-diaminonaphthalene assay is routinely used in the determination of nitrite/nitrate levels in biological fluids and cellular extracts as one indicator of nitric oxide activity. Several laboratories, including ours, have noted matrix-based ... More
A reassessment of the genetic determinants, the effect of growth conditions and the availability of an electron donor on the nitrosating activity of Escherichia coli K-12.
AuthorsMetheringham R, Cole JA
JournalMicrobiology
PubMed ID9274018
'Anaerobic, but not aerobic, cultures of Escherichia coli K-12 catalysed the rapid nitrosation of the model substrate 2,3-diaminonaphthalene when incubated with nitrite. Formate and lactate were effective electron donors for the nitrosation reaction, which was inhibited by nitrate. Optimal growth conditions for the expression of nitrosation activity by various strains ... More
A simple HPLC-fluorescence detection of nitric oxide in cultivated plant cells by in situ derivatization with 2,3-diaminonaphthalene.
AuthorsWada M, Morinaka C, Ikenaga T, Kuroda N, Nakashima K
JournalAnal Sci
PubMed ID12083545
'An HPLC method with fluorescence detection for the determination of nitric oxide (NO) in cultivated plant cells (Agave pacifica, Agavaceae) was developed. NO was derivatized in situ with 2,3-diaminonaphthalene (DAN) as a labeling reagent and converted to 1(H)-naphthotriazole. The maximum peak height of the derivative was observed by incubation for ... More
Fluorometric determination of selenium in nanogram amounts in biological materials using 2,3-diaminonaphthalene.
AuthorsHasunuma R, Ogawa T, Kawanishi Y
JournalAnal Biochem
PubMed ID6297332
Determination of nitric oxide using fluorescence spectroscopy.
AuthorsMiles AM, Wink DA, Cook JC, Grisham MB
JournalMethods Enzymol
PubMed ID8782577
Preliminary studies of the enhanced electrochemiluminescence of 2,3-diaminonaphthalene in the presence of specific metal ions.
AuthorsBruno JG
JournalJ Biolumin Chemilumin
PubMed ID9693981
Electrochemiluminescence (ECL) of 200 ppm 2,3-diaminonaphthalene (2,3-DAN) was studied alone and in conjunction with 100 ppm of 34 different metal and non-metal ions and revealed three relatively intense ECL responses from interactions of 2,3-DAN with Au+, Fe+3 and V+5. ECL responses from Cr+6 or Ru+3 with 2,3-DAN were less intense, ... More
Selenium status in fibromyalgia.
AuthorsReinhard P, Schweinsberg F, Wernet D, Kötter I
JournalToxicol Lett
PubMed ID9820664
Fibromyalgia (FM) is a chronic musculoskeletal pain syndrome of unknown etiology. The serum concentration of selenium (Se) was measured in 68 consecutive patients (nine male, mean age: 47 years; 59 female, mean age 49 years) with FM. The age- and sex-matched control group included 97 female healthy blood donors (mean ... More
Bacterial catalysis of nitrosation: involvement of the nar operon of Escherichia coli.
AuthorsRalt D, Wishnok JS, Fitts R, Tannenbaum SR
JournalJ Bacteriol
PubMed ID3275620
We have developed a rapid and sensitive fluorimetric method, based on the formation of a fluorescent product from nitrosation of 2,3-diaminonaphthalene, for measuring the ability of bacteria to catalyze nitrosation of amines. We have shown in Escherichia coli that nitrosation can be induced under anaerobic conditions by nitrite and nitrate, ... More
Intensity-independent fluorometric detection of cellular nitric oxide release.
AuthorsAndrew PJ, Auer M, Lindley IJ, Kauffmann HF, Kungl AJ
JournalFEBS Lett
PubMed ID9188785
A new fluorescence method is introduced in which nitric oxide (NO)-derived higher-order oxygen complexes (NO(x)) are quantified at physiological pH. Detecting the fluorescence lifetime shift between 2,3-diaminonaphthalene and the NO(x)-derived protonated 2,3-naphthotriazole allows an intensity independent determination of the NO(x) concentration. The NO release from LPS and IFNgamma-stimulated murine macrophages ... More
Fluorometric measurement of nitrite/nitrate by 2,3-diaminonaphthalene.
AuthorsNussler AK, Glanemann M, Schirmeier A, Liu L, Nüssler NC
JournalNat Protoc
PubMed ID17406460
We describe a step-by-step protocol for measuring the stable products of the nitric oxide (NO) pathway: nitrite, nitrite plus nitrate and nitrate. This described protocol is easy to apply and is about 50 times more sensitive than the commonly used Griess reaction or commercially available assay kits based on the ... More
Mechanism for nitrosation of 2,3-diaminonaphthalene by Escherichia coli: enzymatic production of NO followed by O2-dependent chemical nitrosation.
AuthorsJi XB, Hollocher TC
JournalAppl Environ Microbiol
PubMed ID3046492
The mechanism by which Escherichia coli can catalyze the nitrite-dependent nitrosation of 2,3-diaminonaphthalene (DAN), with formation of the corresponding fluorescent triazole, was studied. The reaction was dependent on production of a gaseous compound which can nitrosylate DAN upon contact with air. This compound was identified as nitric oxide (NO), and ... More
Inhibition of endothelial nitric-oxide synthase by ceruloplasmin.
AuthorsBianchini A, Musci G, Calabrese L
JournalJ Biol Chem
PubMed ID10400645
The plasma copper protein ceruloplasmin (CP) was found to inhibit endothelial nitric-oxide synthase activation in cultured endothelial cells, in line with previous evidence showing that the endothelium-dependent vasorelaxation of the aorta is impaired by physiological concentrations of ceruloplasmin. The data presented here indicate a direct relationship between the extent of ... More
Monitoring of feeds selenium status in a southeast region of Romania.
AuthorsSerdaru M, Vladescu L, Avram N
JournalJ Agric Food Chem
PubMed ID14705904
One hundred and eighty-five samples of feeds (hays, green plants, and concentrate feed) from households covering 41 localities in Dobrudja, a southeast region of Romania, were collected and analyzed for selenium (Se) content by spectrofluorometry with 2,3-diaminonaphthalene. Only 6.5% of the samples analyzed were, in terms of the feed Se ... More
Improved nitric oxide detection using 2,3-diaminonaphthalene and its application to the evaluation of novel nitric oxide synthase inhibitors.
AuthorsNakatsubo N, Kojima H, Sakurai K, Kikuchi K, Nagoshi H, Hirata Y, Akaike T, Maeda H, Urano Y, Higuchi T, Nagano T
JournalBiol Pharm Bull
PubMed ID9881632
A specific and sensitive detection method for nitric oxide (NO) in living cells and tissue culture systems is required in the search for novel NO synthase (NOS) inhibitors. We have improved a fluorometric determination with 2,3-diaminonaphthalene (DAN) by the addition of 2-phenyl-4,4,5,5-tetramethylimidazoline-3-oxide-1-oxyl (PTIO) as an oxidant to form NO2 from ... More
Practical methods for detection of nitric oxide.
AuthorsNagano T
JournalLuminescence
PubMed ID10602296
Nitric oxide (NO), which is generated in vivo through conversion of L-arginine to L-citrulline by NO synthase (NOS), mediates many physiological and pathophysiological processes. At least two distinct isoforms of NOS have been identified, constitutive NOS (cNOS) and inducible NOS (iNOS). The cNOS, which is constitutively expressed in endothelial cells ... More
Determination of selenium in human blood by high-performance liquid chromatography with fluorescence detection.
AuthorsHandelman GJ, Kosted P, Short S, Dratz EA
JournalAnal Chem
PubMed ID2596697
Selenium was determined in human whole blood or red cells by high-performance liquid chromatography (HPLC) using a new internal standard. The method provides a 0.15-ng detection limit, a between-day standard deviation of 1% at the 20-ng level, and a 3% within-day standard deviation at the 1-ng level. Samples were wet-ashed, ... More
Fluorometric determination of sulfite and nitrite in aqueous samples using a novel detection unit of a microfluidic device.
AuthorsFujii S, Tokuyama T, Abo M, Okubo A
JournalAnal Sci
PubMed ID14753285
On-chip fluorescence determination of sulfite and nitrite with N-(9-acridinyl)maleimide (NAM) and 2,3-diaminonaphthalene (DAN) has been developed using a novel fluorescence detection unit for microchip analysis. Usually, these fluorescence reagents are derivatized and detected separately in microchip analysis because different fluorescence wavelengths are emitted. The proposed fluorescence detection unit has optical ... More
Simplified fluorometric assay of total selenium in plasma and urine.
AuthorsSheehan TM, Gao M
JournalClin Chem
PubMed ID2253359
We describe here a single-tube assay that may be applied to the whole range of selenium status in adult and pediatric patients, including depletion during parenteral or other nutrition. A specimen or aqueous standard, 100 microL, is digested with 0.5 mL of HNO3/HCIO4 (4/1 by vol, at 190 degrees C ... More
Increase in three alpha,beta-dicarbonyl compound levels in human uremic plasma: specific in vivo determination of intermediates in advanced Maillard reaction.
AuthorsOdani H, Shinzato T, Matsumoto Y, Usami J, Maeda K
JournalBiochem Biophys Res Commun
PubMed ID10066428
Methylglyoxal (MGO), glypxal (GO) and 3-deoxyglucosone (3-DG) are reactive alpha,beta-dicarbonyl intermediates in advanced Maillard reaction, which form advanced glycation and oxidation end products (AGEs) by reaction with both lysine and arginine residues in protein. We measured these three dicarbonyl compound levels in human plasma to estimate the relationship between accumulation ... More
Measurement of 4,5-dioxovaleric acid by high-performance liquid chromatography and fluorescence detection.
AuthorsUmmus RE, Onuki J, Dörnemann D, Medeiros MH, Di Mascio P
JournalJ Chromatogr B Biomed Sci Appl
PubMed ID10410948
In this work we describe a sensitive method for the detection of 4,5-dioxovaleric acid (DOVA). 4,5-Dioxovaleric acid is derivatized with 2,3-diaminonaphthalene to form 3-(benzoquinoxalinyl-2)propionic acid (BZQ), a product with favorable UV absorbance and fluorescence properties. The high-performance liquid chromatographic method with UV absorbance and fluorescence detection is simple and its ... More
The suppression of the N-nitrosating reaction by chlorogenic acid.
AuthorsKono Y, Shibata H, Kodama Y, Sawa Y
JournalBiochem J
PubMed ID8554543
N-Nitrosation of a model aromatic amine (2,3-diamino-naphthalene) by the N-nitrosating agent produced by nitrite in acidic solution was inhibited by a polyphenol, chlorogenic acid, which is an ester of caffeic acid quinic acid. Caffeic acid also inhibited the N-nitrosation, but quinic acid did not. 1,2-Benzenediols and 3,4-dihydroxybenzoic acid had inhibitory ... More
Determination of selenium in human serum by liquid chromatography/electron capture atmospheric pressure chemical ionization mass spectrometry after acid digestion and derivatization using 2,3-diaminonaphthalene.
AuthorsAndo M, Takizawa M, Suwabe S, Yamato S, Shimada K
JournalEur J Mass Spectrom (Chichester, Eng)
PubMed ID15100472
Analysis of selenium in biological samples is very important and numerous analytical methods for the element have been developed. One of the most convenient and widely used methods for routine determination of serum selenium is a fluorometric method using 2,3-diaminonaphthalene (DAN); however, this method lacks specificity. We observed that 4,5-benzopiazselenol ... More
Macrophage triggering with cecropin A and melittin-derived peptides induces type II nitric oxide synthase expression.
AuthorsVelasco M, Díaz-Guerra MJ, Díaz-Achirica P, Andreu D, Rivas L, Boscá L
JournalJ Immunol
PubMed ID9127009
Triggering of RAW 264.7 cells with a cecropin A-melittin hybrid peptide (CA(1-8)M(1-18)) promoted a rapid rise in the intracellular calcium concentration that was followed, after a lag period of 6 h, by nitric oxide synthesis through the expression of the cytokine-inducible form of nitric oxide synthase (type II NOS or ... More
Detection of endothelial nitric oxide release with the 2,3-diaminonapthalene assay.
AuthorsKleinhenz DJ, Fan X, Rubin J, Hart CM
JournalFree Radic Biol Med
PubMed ID12654474
The reliable measurement of nitric oxide (NO) production by endothelial cells in vitro has become an important tool for investigating mechanisms of endothelial dysfunction. This study evaluates measuring NO production by cultured porcine pulmonary artery endothelial cells (PAEC) using the assay based on the fluorometric detection of 1-(H)-naphthotriazole, the fluorescent ... More
Formation of peroxynitrite from reaction of nitroxyl anion with molecular oxygen.
AuthorsKirsch M, de Groot H
JournalJ Biol Chem
PubMed ID11799109
Peroxynitrite (ONOO(-)/ONOOH) is generally expected to be formed in vivo from the diffusion-controlled reaction between superoxide (O(2)) and nitric oxide ((*)NO). In the present paper we show that under aerobic conditions the nitroxyl anion (NO(-)), released from Angeli's salt (disodium diazen-1-ium-1,2,2-triolate, (-)ON=NO(2)(-)), generated peroxynitrite with a yield of about 65%. ... More
PH Control in the fluorometric assay for selenium with 2,3-diaminonaphthalene.
AuthorsBayfield RF, Romalis LF
JournalAnal Biochem
PubMed ID3993917
Inconsistencies in reproducibility and duplication in the fluorometric assay of selenium in biological materials with 2,3-diaminonaphthalene (DAN) were investigated. These were attributed to inadequate pH control in the reaction mixture following acid digestion of samples and preceding formation of the piazselenol complex. The problems were overcome by using methyl orange ... More
Improved molecular fluorescence method for the determination of selenium in biological samples.
AuthorsHarrison I, Littlejohn D, Fell GS
JournalAnalyst
PubMed ID8952451
A procedure for the determination of selenium in whole blood and urine has been improved by optimization of the digestion and derivatization procedures. An overnight pre-digestion step with 4 + 1 concentrated nitric-perchloric acids reduced the time of mineralization at 170 degrees C from 4 h to 30-45 min. Conversion ... More
Non-selenium glutathione peroxidase without glutathione S-transferase activity from bovine ciliary body.
AuthorsShichi H, Demar JC
JournalExp Eye Res
PubMed ID2373154
A glutathione peroxidase was purified from bovine ciliary body by ammonium sulfate fractionation. Sephacryl S-300 gel filtration, diethylaminoethyl (DEAE)-cellulose chromatography and hydroxyapatite chromatography. The purified enzyme has an apparent mw of 112 kDa by gel filtration and 29 kDa by SDS-polyacrylamide gel electrophoresis. The enzyme therefore is composed of four ... More
Unexpected reaction pathways in the reaction of alkoxyalkynylchromium(0) carbenes with aromatic dinucleophiles.
AuthorsSierra MA, Mancheño MJ, del Amo JC, Fernández I, Gómez-Gallego M
JournalChemistry
PubMed ID14562313
Thermal- or SiO(2)-induced reactions of the Michael adducts of 1,2-aromatic dinucleophiles and alkynylchromium(0) carbene complexes, compounds 7-10, form different products in good yields depending on the nature of the aromatic dinucleophile used. Thus, 1,2-diaminobenzene derivatives 7 and 8 rearrange to pentacarbonylchromium(0) isocyanide complexes 11, 12, 14, and 15 in a ... More
Simple and specific reversed-phase liquid chromatographic method with diode-array detection for simultaneous determination of serum hydroxychloroquine, chloroquine and some corticosteroids.
AuthorsVolin P
JournalJ Chromatogr B Biomed Appl
PubMed ID7633613
This paper describes a simple, specific, and sensitive high-performance liquid chromatographic (HPLC) method using ion-pair reversed-phase chromatography with photodiode-array detection for the simultaneous determination of hydroxychloroquine (HCQ) and chloroquine (CQ) in serum samples from rheumatoid arthritis patients receiving either HCQ sulphate or CQ diphosphate. The assay is also applicable to ... More
An apoptotic model for nitrosative stress.
AuthorsEu JP, Liu L, Zeng M, Stamler JS
JournalBiochemistry
PubMed ID10653649
Nitric oxide overproduction has been implicated in the pathogenesis of many disorders, including artherosclerosis, neurodegenerative diseases, inflammatory and autoimmune diseases, and cancer. The common view holds that nitric oxide-induced cellular injury is caused by oxidative stress. This theory predicts that interactions between reactive nitrogen species and reactive oxygen species produce ... More
Fluorometric assay of nitrite and nitrate in brain tissue after traumatic brain injury and cerebral ischemia.
AuthorsRao AM, Dogan A, Hatcher JF, Dempsey RJ
JournalBrain Res
PubMed ID9630667
Nitric oxide synthase (NOS) is distributed within the brain, and nitric oxide (NO) is felt to be involved in the pathophysiology of deterioration after head injury and cerebral ischemia. This study determined the levels of the stable end products of NOS (NOx=nitrite+nitrate) after traumatic brain injury (TBI) and transient cerebral ... More
Nitrosative capacity of macrophages is dependent on nitric-oxide synthase induction signals.
AuthorsEspey MG, Miranda KM, Pluta RM, Wink DA
JournalJ Biol Chem
PubMed ID10753947
Nitrosative stress can occur when reactive nitric oxide (NO) species compromise the function of biomolecules via formation of NO adducts on critical amine and thiol residues. The capacity of inducible nitric-oxide synthase (iNOS) to generate nitrosative stress was investigated in the murine macrophage line ANA-1. Sequential activation with the cytokines ... More
Effects of glycemic control on plasma 3-deoxyglucosone levels in NIDDM patients.
AuthorsHamada Y, Nakamura J, Fujisawa H, Yago H, Nakashima E, Koh N, Hotta N
JournalDiabetes Care
PubMed ID9283799
OBJECTIVE: To clarify the effects of glycemic control on the level of 3-deoxyglucosone (3-DG), a reactive dicarbonyl compound, in plasma from diabetic patients. RESEARCH DESIGN AND METHODS: Fasting plasma samples were collected from 15 healthy volunteers and 27 patients with NIDDM. Samples were collected from six poorly controlled patients before ... More
TNFalpha and oxLDL reduce protein S-nitrosylation in endothelial cells.
AuthorsHoffmann J, Haendeler J, Zeiher AM, Dimmeler S
JournalJ Biol Chem
PubMed ID11524431
Nitric oxide (NO) plays an important role in the regulation of the functional integrity of the endothelium. The intracellular reaction of NO with reactive cysteine groups leads to the formation of S-nitrosothiols. To investigate the regulation of S-nitrosothiols in endothelial cells, we first analyzed the composition of the S-nitrosylated molecules ... More
Inhibition of glutathione reductase by dinitrosyl-iron-dithiolate complex.
AuthorsBoese M, Keese MA, Becker K, Busse R, Mülsch A
JournalJ Biol Chem
PubMed ID9268306
The biological signal molecule nitric oxide (NO) exists in a free and carrier-bound form. Since the structure of the carrier is likely to influence the interaction of NO with macromolecular targets, we assessed the interaction of a dinitrosyl-iron-dithiolate complex carrying different thiol ligands with glutathione reductase. The enzyme was irreversibly ... More
The oxidative and nitrosative chemistry of the nitric oxide/superoxide reaction in the presence of bicarbonate.
AuthorsJourd'heuil D, Miranda KM, Kim SM, Espey MG, Vodovotz Y, Laroux S, Mai CT, Miles AM, Grisham MB, Wink DA
JournalArch Biochem Biophys
PubMed ID10222043
The primary product of the interaction between nitric oxide (NO) and superoxide () is peroxynitrite (ONOO-), which is capable of either oxidizing or nitrating various biological substrates. However, it has been shown that excess NO or can further react with ONOO- to form species which mediate nitrosation. Subsequently, the controlled ... More
Increase in 3-deoxyglucosone levels in diabetic rat plasma. Specific in vivo determination of intermediate in advanced Maillard reaction.
AuthorsYamada H, Miyata S, Igaki N, Yatabe H, Miyauchi Y, Ohara T, Sakai M, Shoda H, Oimomi M, Kasuga M
JournalJ Biol Chem
PubMed ID8051120
A specific assay of 3-deoxyglucosone (3-DG) was developed in our laboratory to help elucidate the relationship between advanced Maillard reaction and diabetic complications. 3-DG is known as a highly reactive intermediate of the reaction in vitro and a precursor of advanced glycosylation end products such as pyrraline and pentosidine, which ... More
High glucose-induced oxidative stress causes apoptosis in proximal tubular epithelial cells and is mediated by multiple caspases.
AuthorsAllen DA, Harwood S, Varagunam M, Raftery MJ, Yaqoob MM
JournalFASEB J
PubMed ID12670885
Diabetic nephropathy is the leading cause of end-stage renal disease in the Western world. Poor glycemic control contributes to the development of diabetic nephropathy, but the mechanisms underlying high glucose-induced tissue injury are not fully understood. In the present study, the effect of high glucose on a proximal tubular epithelial ... More
Microwave digestion of environmental and natural waters for selenium speciation.
AuthorsWang Z, Gao YX, Belzile N
JournalAnal Chem
PubMed ID11605851
A microwave preparation procedure is proposed for selenium speciation in natural and drinking waters. Different chemical reagents were tested, and the conditions for Se speciation were optimized. The effect of the different reagents on various oxidation states of selenium under microwave digestion conditions was investigated. Most of the Se(-II) was ... More
Fluorometric detection of biological S-nitrosothiols.
AuthorsPark JK, Kostka P
JournalAnal Biochem
PubMed ID9193709
A technique is presented for the quantitative detection of S-nitrosothiols formed by model biological thiols, cysteine, glutathione, and serum albumin. The technique is based on the detection of fluorescent compound 1-[H]-naphthotriazole formed between 2,3-diaminonaphthalene and nitrous acid released from S-nitrosothiols by treatment with mercuric chloride in an acidic environment. Concentration ... More
Activation of phosphatidylinositol 3-kinase by interleukin-13. An inhibitory signal for inducible nitric-oxide synthase expression in epithelial cell line HT-29.
AuthorsWright K, Ward SG, Kolios G, Westwick J
JournalJ Biol Chem
PubMed ID9139718
The human colonic epithelial cell line HT-29 can be induced by a combination of the cytokines interleukin (IL)-1alpha, tumor necrosis factor alpha, and interferon-gamma to express the inducible form of nitric-oxide synthase (iNOS; Kolios, G., Brown, Z., Robson, R., Robertson, D. A. F., & Westwick, J. (1995) Br. J. Pharmacol. ... More
Quantitation of 3-deoxyglucosone levels in human plasma.
AuthorsLal S, Kappler F, Walker M, Orchard TJ, Beisswenger PJ, Szwergold BS, Brown TR
JournalArch Biochem Biophys
PubMed ID9186486
3-Deoxyglucosone (3DG), a reactive dicarbonyl, is an important intermediate in the formation of advanced glycation end products (AGEs). The AGEs are particularly important in diabetes since they have been correlated with the development of diabetic complications. Consequently, measurements of 3DG are likely to provide valuable insights into the role of ... More
Selenium supplementation on plasma glutathione peroxidase activity in patients with end-stage chronic renal failure.
AuthorsZachara BA, Koterska D, Manitius J, Sadowski L, Dziedziczko A, Salak A, Wasowicz W
JournalBiol Trace Elem Res
PubMed ID14742897
Patients with chronic renal failure (CRF) usually have a lower than healthy level of selenium (Se) in whole blood and plasma. Plasma glutathione peroxidase (GSH-Px) is synthesized mostly in the kidney. In CRF patients, activity of this enzyme is significantly reduced and its reduction increases with the progress of the ... More
Effects of aminosalicylates and immunosuppressive agents on nitric oxide-dependent N-nitrosation reactions.
AuthorsGrisham MB, Miles AM
JournalBiochem Pharmacol
PubMed ID8204107
Recent studies have demonstrated that nitric oxide (NO) rapidly and spontaneously decomposes in oxygenated solutions to generate potent N-nitrosating agents. These electrophilic substances have been shown to mediate mutagenesis and carcinogenesis via the formation of aliphatic and aromatic nitrosamines. We have also demonstrated that extravasated neutrophils and macrophages produce significant ... More
Improved methods to measure end products of nitric oxide in biological fluids: nitrite, nitrate, and S-nitrosothiols.
AuthorsMarzinzig M, Nussler AK, Stadler J, Marzinzig E, Barthlen W, Nussler NC, Beger HG, Morris SM, Brückner UB
JournalNitric Oxide
PubMed ID9701056
The aim of this study was to compare and improve standard methods to determine nitrite (NO2-), nitrate (NO3-) and S-nitrosothiol (RSNO) levels in cell culture supernatants, sera, and urine. We modified the conventional Griess reaction by replacing sulfanilamide with dapsone (4,4'-diamino-diphenylsulfone) and compared the NO2- levels in our study samples ... More
Acid-promoted tautomeric lactonization and oxidation-reduction of pyrroloquinoline quinone (PQQ).
AuthorsGallop PM, Henson E, Paz MA, Greenspan SL, Flückiger R
JournalBiochem Biophys Res Commun
PubMed ID2551275
Acid-treatment facilitates PQQ detection by electron ionization mass spectroscopy with a molecular ion at M/e 330 and a base ion formed by triple decarboxylation at M/e 198. Other ions found probably arise through acid-catalyzed tautomeric lactonization of PQQ to PQQ-lactone (PQQL) with subsequent oxidation of PQQL and reduction of PQQ. ... More
Nitrite-induced nitration pathways of retinoic acid, 5,6-epoxyretinoic acid, and their esters under mildly acidic conditions: toward a reappraisal of retinoids as scavengers of reactive nitrogen species.
AuthorsPanzella L, Manini P, Crescenzi O, Napolitano A, d'Ischia M
JournalChem Res Toxicol
PubMed ID12703967
All trans retinoic acid (1), a cancer chemopreventive agent and a pluripotent morphogen, was found to react efficiently with nitrite ions in a biphasic system consisting of CH(2)Cl(2)/0.1 M phosphate buffer (pH 3) 1:1 v/v to give a complex mixture of nitration products. Repeated TLC fractionation of the reaction mixtures ... More
Convenient colorimetric and fluorometric assays for S-nitrosothiols.
AuthorsCook JA, Kim SY, Teague D, Krishna MC, Pacelli R, Mitchell JB, Vodovotz Y, Nims RW, Christodoulou D, Miles AM, Grisham MB, Wink DA
JournalAnal Biochem
PubMed ID8660604
S-nitrosothiols have been shown to affect a number of physiological functions. Several techniques have been used to detect these species in biological systems, primarily by methods utilizing chemiluminescence. Since the apparatus required for measurement of chemiluminescence are not readily available in most laboratories, methods employing more conventional techniques such as ... More
Inhibition of glycosphingolipid synthesis by threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol (PDMP) and the modulation of IL-1beta-stimulated expression of inducible nitric oxide synthase in rat aortic smooth muscle cells.
AuthorsWeber AA, Seul C, Harth V, Ko Y, Seewald S, Vetter H, Sachinidis A
JournalBr J Pharmacol
PubMed ID9535019
1. The composition of glycosphingolipids is altered in atherosclerotic tissue. In order to study the possible modulation of interleukin-1beta (IL-1beta)-induced expression of inducible nitric oxide synthase (iNOS) by endogenously synthesized glycosphingolipids, we investigated rat aortic vascular smooth muscle cells (VSMC) grown in the presence of the inhibitor of glycosphingolipid synthesis, ... More
Induction of nitric oxide in human monocytes and monocyte cell lines by Mycobacterium tuberculosis.
AuthorsJagannath C, Actor JK, Hunter RL
JournalNitric Oxide
PubMed ID9731635
The induction of nitric oxide in human monocytes during mycobacterial infection has been a controversial issue. This study describes a comparative evaluation of the colorimetric and fluorometric methods for the detection of NO in response to Mycobacterium tuberculosis (MTB) infection in human peripheral blood-derived monocytes (PBM) and in U937, a ... More