Reactive oxygen species production via NADPH oxidase mediates TGF-beta-induced cytoskeletal alterations in endothelial cells.
AuthorsHu T, Ramachandrarao SP, Siva S, Valancius C, Zhu Y, Mahadev K, Toh I, Goldstein BJ, Woolkalis M, Sharma K
JournalAm J Physiol Renal Physiol
PubMed ID16159901
'Cytoskeletal alterations in endothelial cells have been linked to nitric oxide generation and cell-cell interactions. Transforming growth factor (TGF)-beta has been described to affect cytoskeletal rearrangement in numerous cell types; however, the underlying pathway is unclear. In the present study, we found that human umbilical vein endothelial cells (HUVEC) have ... More
Simultaneous visualization of G- and F-actin in endothelial cells.
AuthorsHaugland RP, You W, Paragas VB, Wells KS, DuBose DA
JournalJ Histochem Cytochem
PubMed ID8308251
'We developed site-specific fluorescent probes that permit simultaneous microscopic observation of G- and F-actin in bovine endothelial cells. G-actin distribution was visualized with fluorescein-deoxyribonuclease I (DNAse I). F-actin was labeled with phalloidin conjugated to the new long-wavelength fluorophore BODIPY 581/591 (581-nm excitation, 591-nm emission), which is spectrally similar to Texas ... More
Simultaneous localization and quantification of relative G and F actin content: optimization of fluorescence labeling methods.
AuthorsKnowles GC, McCulloch CA
JournalJ Histochem Cytochem
PubMed ID1527379
'Previous studies of fluorescence probes for labeling the monomeric actin pool have demonstrated lack of specificity. We have used quantitative analytical methods to assess the sensitivity and specificity of rhodamine DNAse I as a probe for monomeric (G) actin. The G-actin pool of attached or suspended fibroblasts was stabilized by ... More
Comparisons of endothelial cell G- and F-actin distribution in situ and in vitro.
AuthorsDuBose DA, Haugland R
JournalBiotech Histochem
PubMed ID8448251
'Numerous studies have described the F-actin cytoskeleton; however, little information relevant to G-actin is available. The actin pools of bovine aortic endothelial cells were examined using in situ and in vitro conditions and fluorescent probes for G-(deoxyribonuclease I, 0.3 microM) or F-actin (phalloidin, 0.2 microM). Cells in situ displayed a ... More
Role of actin in the responses of adrenal cells to ACTH and cyclic AMP: inhibition by DNase I.
AuthorsOsawa S, Betz G, Hall PF
JournalJ Cell Biol
PubMed ID6090470
'Erythrocyte ghosts were loaded with pancreatic DNase I and fused with Y-1 adrenal tumor cells to test the possibility that this enzyme might inhibit the steroidogenic responses of the cells to ACTH and cyclic AMP. Fusion of erythrocyte ghosts loaded with DNase I, but not those containing albumin, ovalbumin, boiled ... More
Effects of exogenous proteins on cytoplasmic streaming in perfused Chara cells.
AuthorsNothnagel EA, Sanger JW, Webb WW
JournalJ Cell Biol
PubMed ID6126482
'Cytoplasmic streaming in characean algae is thought to be generated by interaction between subcortical actin bundles and endoplasmic myosin. Most of the existing evidence supporting this hypothesis is of a structural rather than functional nature. To obtain evidence bearing on the possible function of actin and myosin in streaming, we ... More
Localization of actin in the cortex of Paramecium tetraurelia cells by immuno- and affinity-fluorescence microscopy.
AuthorsTiggemann R, Plattner H
JournalEur J Cell Biol
PubMed ID7285935
'The cortex of the ciliate Paramecium tetraurelia has been examined both with whole cells and with isolated surface complexes (pellicles) for the presence and distribution of actin, using direct fluorescence labeling with antibodies monospecific for Paramecium actin, with DNase I or with heavy meromyosin, all tagged with FITC as a ... More
Affinity cytochemistry: the localization of lectin and antibody receptors on erythrocytes via the avidin-biotin complex.
AuthorsBayer EA, Wilchek M, Skutelsky E
JournalFEBS Lett
PubMed ID976475
Mechanical fluctuations of the membrane-skeleton are dependent on F-actin ATPase in human erythrocytes.
AuthorsTuvia S, Levin S, Bitler A, Korenstein R
JournalJ Cell Biol
PubMed ID9647648
Cell membrane fluctuations (CMF) of human erythrocytes, measured by point dark field microscopy, were shown to depend, to a large extent, on intracellular MgATP (Levin, S.V., and R. Korenstein. 1991. Biophys. J. 60:733-737). The present study extends that investigation and associates CMF with F-actin's ATPase activity. MgATP was found to ... More
A 133Cs nuclear magnetic resonance study of endothelial Na(+)-K(+)-ATPase activity: can actin regulate its activity?
AuthorsGruwel ML, Culíc O, Schrader J
JournalBiophys J
PubMed ID9168052
Using (133)Cs+ NMR, we developed a technique to repetitively measure, in vivo, Na(+)-K(+)-ATPase activity in endothelial cells. The measurements were made without the use of an exogenous shift reagent, because of the large chemical shift of 1.36 +/- 0.13 ppm between intra- and extracellular Cs+. Intracellularly we obtained a spin ... More
Mesangial cell actin disassembly in high glucose mediated by protein kinase C and the polyol pathway.
AuthorsZhou X, Li C, Dlugosz J, Kapor-Drezgic J, Munk S, Whiteside C
JournalKidney Int
PubMed ID9186869
High glucose alters mesangial cell cytoskeletal structure and function. We postulated that high glucose causes mesangial cell filamentous (F) actin disassembly through a protein kinase C (PKC) mechanism involving the polyol pathway. Rat mesangial cells (passage < 10, N = 60/group) were growth-arrested and then cultured in glucose 5.6 mM ... More
High glucose alters actin assembly in glomerular mesangial and epithelial cells.
AuthorsZhou X, Hurst RD, Templeton D, Whiteside CI
JournalLab Invest
PubMed ID7564270
BACKGROUND: Glomerular mesangial and epithelial cell structure and function are maintained by cytoskeletal protein organization and function. To determine whether the diabetic milieu alters filamentous (F-) actin assembly, the spatial distributions and content of F- and monomeric (G-) actin were analyzed in rat mesangial and glomerular epithelial cells (10 to ... More
The complex of actin and deoxyribonuclease I as a model system to study the interactions of nucleotides, cations and cytochalasin D with monomeric actin.
AuthorsPolzar B, Nowak E, Goody RS, Mannherz HG
JournalEur J Biochem
PubMed ID2500340
The stoichiometric actin--DNase-I complex was used to study the actin--nucleotide and actin--divalent-cation interactions and its ATPase activity in the presence of MgCl2 and cytochalasin D. Treatment of actin--DNase-I complex with 1 mM EDTA results in almost complete restoration of its otherwise inhibited DNase I activity, although the complex does not ... More