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引用和文献 (225)
Invitrogen™
同二聚乙胺-1 (EthD-1)
细胞不可透过性活力指示剂乙锭同源二聚体-1 (EthD-1) 是一种高亲和力核酸染色剂，与 DNA 结合前发弱荧光，与 DNA 结合后发红色荧光（最大激发/发射波长为 ∼528/617）了解更多信息
| 货号 | 数量 |
|---|---|
| E1169 | 1 mg |
货号 E1169
价格（CNY)
4,995.00
飞享价
Ends: 31-Dec-2025
6,771.00共减 1,776.00 (26%)
Each
数量:
1 mg
价格（CNY)
4,995.00
飞享价
Ends: 31-Dec-2025
6,771.00共减 1,776.00 (26%)
Each
细胞不可透过性活力指示剂乙锭同源二聚体-1 (EthD-1) 是一种高亲和力核酸染色剂，与 DNA 结合前发弱荧光，与 DNA 结合后发红色荧光（最大激发/发射波长为 ∼528/617）。我们建议使用高质量的无水 DMSO，在 DMSO/H2O 1:4 (v/v) 中制备 1-2 mM 的储备液。
仅供科研使用。不可用于诊断程序。
规格
颜色橙色
描述乙锭同源二聚体-1 (EthD-1)
检测方法荧光
染料类型细胞不可透过性
发射617 nm
激发波长范围528 nm
适用于（应用）细胞染色测定
适用于（设备）荧光显微镜
数量1 mg
运输条件室温
标签类型Fluorescent Dye
产品类型乙锭同源二聚体-1
亚细胞定位细胞核,核酸, Nucleus
Unit SizeEach
内容与储存
在冷柜(-5°C 至 -30°C)中避光储存。
常见问题解答 (FAQ)
I am doing a Live/Dead assay using Calcein, AM, for live cells and ethidium homodimer-1 for dead cells. Can I fix the cells after labeling and retain the staining?
引用和文献 (225)
引用和文献
Abstract
Enzymatic isolation and characterization of single vascular smooth muscle cells from cremasteric arterioles.
Journal:Microcirculation (New York, N.Y. : 1994)
PubMed ID:9110282
OBJECTIVE: The goal of the present study was to develop a method to isolate viable arteriolar muscle cells from single cremasteric arterioles, which retain the contractile and electrophysiological phenotype of the donor microvessels. METHODS: Arterioles were hand-dissected from rat and hamster cremaster muscles and dissociated by incubation in papain and
Enzymatic isolation and characterization of single vascular smooth muscle cells from cremasteric arterioles.
Journal:Microcirculation (New York, N.Y. : 1994)
PubMed ID:8930888
OBJECTIVE: The goal of the present study was to develop a method to isolate enzymatically viable arteriolar muscle cells from single cremasteric arterioles, which retain the contractile and electrophysiological phenotype of the donor microvessels. METHODS: Arterioles were hand-dissected from rat and hamster cremaster muscles and dissociated by incubation in papain
A stable double-stranded DNA-ethidium homodimer complex: application to picogram fluorescence detection of DNA in agarose gels.
Journal:Proc Natl Acad Sci U S A
PubMed ID:2339125
The complex between double-stranded DNA and ethidium homodimer (5,5'-diazadecamethylene)bis(3,8-diamino-6-phenylphenanthridini um) cation, formed at a ratio of 1 homodimer per 4 or 5 base pairs, is stable in agarose gels under the usual conditions for electrophoresis. This unusual stability allows formation of the complex before electrophoresis and then separation and detection
Successful storage of peripheral nerve before transplantation using green tea polyphenol: an experimental study in rats.
Journal:Exp Neurol
PubMed ID:14769360
Green tea polyphenol is known to act as a buffer, reducing biological responses to oxidative stress. Several effects of polyphenol have been reported, such as protection of tissue from ischemia, antineoplasmic and anti-inflammatory effects, and suppression of arteriosclerosis. In this study, we investigated whether peripheral nerve segments could be kept
Indirubin enhances tumor necrosis factor-induced apoptosis through modulation of nuclear factor-kappa B signaling pathway.
Journal:J Biol Chem
PubMed ID:16785236
'Although indirubin is known to exhibit anti-cancer and anti-inflammatory activities, very little is known about its mechanism of action. In this study, we investigated whether indirubin mediates its effects through interference with the NF-kappaB pathway. As examined by the DNA binding of NF-kappaB, we found that indirubin suppressed tumor necrosis