EnzChek™ 磷酸酶检测试剂盒

引用和文献 (4)

Invitrogen™

EnzChek™ 磷酸酶检测试剂盒

使用 EnzChek 磷酸酶检测试剂盒可连续监测磷酸酶活性，即使在中性或酸性 pH 值条件下也能够进行监测。本款试剂盒包含 Molecular Probes 专有的 6,8-二氟-4-甲基伞形酮磷酸酯了解更多信息

货号	数量
E12020	1000 次检测

货号 E12020

价格（CNY)

4,540.00

添加至购物车

1000 次检测

价格（CNY)

4,540.00

添加至购物车

使用 EnzChek 磷酸酶检测试剂盒可连续监测磷酸酶活性，即使在中性或酸性 pH 值条件下也能够进行监测。本款试剂盒包含 Molecular Probes 专有的 6,8-二氟-4-甲基伞形酮磷酸酯 (DiFMUP) 底物 (D6567)。在 pH 值为5.5的条件下检测前列腺酸性磷酸酶时，DiFMUP 的灵敏度比 4-甲基伞形酮磷酸酯 (MUP) 高约100倍。该试剂盒非常适合连续测定前列腺酸性磷酸酶、蛋白磷酸酶1或几乎任何其他可使用非蛋白质底物例如 MUP 或 4-硝基苯磷酸盐 (PNPP) 进行测定的磷酸酶。每个试剂盒均包含足够以 100 µL 的反应体积进行 ∼1000 次测定的底物。荧光（最大激发/发射波长为 ∼358/455 nm）可以使用荧光微孔板读数仪或标准荧光计进行测量。

仅供科研使用。不可用于诊断程序。

检测方法荧光

染料类型DiFMU

数量1000 次检测

运输条件室温

底物DiFMUP

底物属性化学底物

底物类型磷酸酶底物

目标酶磷酸酶

发射360⁄460

适用于（应用）磷酸酶检测

适用于（设备）微孔板读数仪

产品线EnzChek

产品类型磷酸酶检测

Unit SizeEach

内容与储存

在冷冻冰箱（-5°C 至 -30°C）中避光储存。

常见问题解答 (FAQ)

What is the reaction product excitation/emission maxima when using the EnzChek Phosphatase Assay kit?

The reaction product has excitation/emission maxima of approximately 358/455 nm. Fluorescence can be measured in a fluorescence microplate reader or a standard fluorometer.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (4)

引用和文献

Abstract

Plectin scaffolds recruit energy-controlling AMP-activated protein kinase (AMPK) in differentiated myofibres.

Authors:Gregor M, Zeöld A, Oehler S, Marobela KA, Fuchs P, Weigel G, Hardie DG, Wiche G,

Journal:J Cell Sci

PubMed ID:16608880

'Plectin, a cytolinker protein greater than 500 kDa in size, has an important role as a mechanical stabiliser of cells. It interlinks the various cytoskeletal filament systems and anchors intermediate filaments to peripheral junctional complexes. In addition, there is increasing evidence that plectin acts as a scaffolding platform that controls ... More

The Down syndrome critical region protein RCAN1 regulates long-term potentiation and memory via inhibition of phosphatase signaling.

Authors:Hoeffer CA, Dey A, Sachan N, Wong H, Patterson RJ, Shelton JM, Richardson JA, Klann E, Rothermel BA,

Journal:J Neurosci

PubMed ID:18045910

'Regulator of calcineurin 1 (RCAN1/MCIP1/DSCR1) regulates the calmodulin-dependent phosphatase calcineurin. Because it is located on human chromosome 21, RCAN1 has been postulated to contribute to mental retardation in Down syndrome and has been reported to be associated with neuronal degeneration in Alzheimer''s disease. The studies herein are the first to ... More

Adenovirus-facilitated nuclear translocation of adeno-associated virus type 2.

Authors:Xiao W, Warrington KH, Hearing P, Hughes J, Muzyczka N

Journal:J Virol

PubMed ID:12388712

'We examined cytoplasmic trafficking and nuclear translocation of adeno-associated virus type 2 (AAV) by using Alexa Fluor 488-conjugated wild-type AAV, A20 monoclonal antibody immunocytochemistry, and subcellular fractionation techniques followed by DNA hybridization. Our results indicated that in the absence of adenovirus (Ad), AAV enters the cell rapidly and escapes from ... More

Redox modulation of global phosphatase activity and protein phosphorylation in intact skeletal muscle.

Authors:Wright VP, Reiser PJ, Clanton TL,

Journal:J Physiol

PubMed ID:19841000

Skeletal muscles produce transient reactive oxygen species (ROS) in response to intense stimulation, disuse atrophy, heat stress, hypoxia, osmotic stress, stretch and cell receptor activation. The physiological significance is not well understood. Protein phosphatases (PPases) are known to be highly sensitive to oxidants and could contribute to many different signalling ... More