Search
引用和文献 (46)
Invitrogen™
EnzChek™ 明胶酶/胶原酶检测试剂盒，250-2,000 次检测
EnzChek 明胶酶/胶原酶检测试剂盒提供了一种灵敏、便利、快速的荧光测定方法，可用于在经过纯化的酶体系以及细胞/组织裂解物中测量明胶酶或胶原酶活性，或用于以高通量形式筛选抑制剂。EnzChek 试剂盒中的底物是我们使用荧光素标记的 DQ 明胶偶联物，其经过高度标记，以至于荧光信号被淬灭，直到酶消化后才会产生具有强烈荧光的片段。该底物也可以与试剂盒分开提供 (D-12054)了解更多信息
| 货号 | 数量 |
|---|---|
| E12055 | 1 Kit |
货号 E12055
价格（CNY)
6,416.00
Each
数量:
1 Kit
价格（CNY)
6,416.00
Each
EnzChek 明胶酶/胶原酶检测试剂盒提供了一种灵敏、便利、快速的荧光测定方法，可用于在经过纯化的酶体系以及细胞/组织裂解物中测量明胶酶或胶原酶活性，或用于以高通量形式筛选抑制剂。EnzChek 试剂盒中的底物是我们使用荧光素标记的 DQ 明胶偶联物，其经过高度标记，以至于荧光信号被淬灭，直到酶消化后才会产生具有强烈荧光的片段。该底物也可以与试剂盒分开提供 (D-12054)。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光强度
数量1 Kit
运输条件室温
底物属性基于蛋白的底物
底物类型明胶酶⁄胶原酶底物
目标酶明胶酶⁄胶原酶
适用于（应用）明胶酶/胶原酶测定试剂盒
产品线EnzChek
产品类型明胶酶⁄胶原酶检测
Unit SizeEach
内容与储存
在冷冻冰箱（-5°C 至 -30°C）中避光储存。
引用和文献 (46)
引用和文献
Abstract
Gelatin in situ zymography on fixed, paraffin-embedded tissue: zinc and ethanol fixation preserve enzyme activity.
Journal:J Histochem Cytochem
PubMed ID:19755718
In situ zymography is a method for the detection and localization of enzymatic activity in tissue sections. This method is used with frozen sections because routine fixation of tissue in neutral-buffered formalin inhibits enzyme activity. However, frozen sections present with poor tissue morphology, making precise localization of enzymatic activity difficult
Chlorotoxin inhibits glioma cell invasion via matrix metalloproteinase-2.
Journal:J Biol Chem
PubMed ID:12454020
'Primary brain tumors (gliomas) have the unusual ability to diffusely infiltrate the normal brain thereby evading surgical treatment. Chlorotoxin is a scorpion toxin that specifically binds to the surface of glioma cells and impairs their ability to invade. Using a recombinant His-Cltx we isolated and identified the principal Cltx receptor
Authentic matrix vesicles contain active metalloproteases (MMP). a role for matrix vesicle-associated MMP-13 in activation of transforming growth factor-beta.
Journal:J Biol Chem
PubMed ID:11145962
'Matrix vesicles (MV) play a key role in the initiation of cartilage mineralization. Although many components in these microstructures have been identified, the specific function of each component is still poorly understood. In this study, we show that metalloproteases (MMP), MMP-2, -9, and -13 are associated with MV isolated from
Effective antiprotease-antibiotic treatment of experimental anthrax.
Journal:BMC Infect Dis
PubMed ID:15819985
'BACKGROUND: Inhalation anthrax is characterized by a systemic spread of the challenge agent, Bacillus anthracis. It causes severe damage, including multiple hemorrhagic lesions, to host tissues and organs. It is widely believed that anthrax lethal toxin secreted by proliferating bacteria is a major cause of death, however, the pathology of
Molecular proximity of seprase and the urokinase-type plasminogen activator receptor on malignant melanoma cell membranes: dependence on beta1 integrins and the cytoskeleton.
Journal:Carcinogenesis
PubMed ID:12376466
'Previous studies have shown that several proteolytic enzymes are associated with membrane protrusions at the leading edge of migrating tumor cells. In this study we demonstrate that seprase and the urokinase plasminogen activator receptor (uPAR), co-localize in the plasma membrane of LOX malignant melanoma cells. Cells were labeled with fluorochrome-conjugated