Novel small molecule inhibitors of caspase-3 block cellular and biochemical features of apoptosis.
AuthorsScott CW, Sobotka-Briner C, Wilkins DE, Jacobs RT, Folmer JJ, Frazee WJ, Bhat RV, Ghanekar SV, Aharony D
JournalJ Pharmacol Exp Ther
PubMed ID12490620
'Caspase-3 is an intracellular cysteine protease, activated as part of the apoptotic response to cell injury. Its interest as a therapeutic target has led many to pursue the development of inhibitors. To date, only one series of nonpeptidic inhibitors have been described, and these have limited selectivity within the caspase ... More
Role of the sphingosine rheostat in the regulation of cnidarian-dinoflagellate symbioses.
AuthorsDetournay O, Weis VM,
JournalBiol Bull
PubMed ID22186914
'The symbiosis between host cnidarians, such as corals and anemones, and their dinoflagellate symbionts is regulated by largely undescribed mechanisms that stabilize the symbiosis during normal conditions but lead to symbiosis breakdown, or cnidarian bleaching, during stress. Previous transcriptomic studies identified the sphingosine rheostat as a putative symbiosis regulatory pathway. ... More
Fluorescent molecular probes V: a sensitive caspase-3 substrate for fluorometric assays.
(Z-Asp-Glu-Val-Asp)-Rhodamine 110 [(Z-DEVD)2-Rh 110] was prepared and characterized as a sensitive fluorogenic substrate for the determination of caspase-3 activity.
Photodynamic therapy and cell death pathways.
AuthorsKessel D, Oleinick NL,
JournalMethods Mol Biol
PubMed ID20552338
Photodynamic therapy (PDT) is the term used to describe the irradiation of photosensitized cells or tissue with phototoxic consequences. This process can result in the rapid initiation of not only apoptosis, an irreversible death pathway, but also autophagy. The procedures described here are designed to characterize the correlation between the ... More
Rhodamine 110-linked amino acids and peptides as substrates to measure caspase activity upon apoptosis induction in intact cells.
AuthorsHug H, Los M, Hirt W, Debatin KM
JournalBiochemistry
PubMed ID10529236
Caspases (cysteine aspartate-specific proteases) are a structurally related group of cysteine proteases that cleave peptide bonds following specific recognition sequences. They play a central role in activating apoptosis of vertebrate cells. To measure apoptosis induced by various stimuli and at an early apoptotic stage, caspases are an ideal target. This ... More
Identification of BARD1 as mediator between proapoptotic stress and p53-dependent apoptosis.
AuthorsIrminger-Finger I, Leung WC, Li J, Dubois-Dauphin M, Harb J, Feki A, Jefford CE, Soriano JV, Jaconi M, Montesano R, Krause KH
JournalMol Cell
PubMed ID11779501
The BRCA1-associated protein BARD1 is a putative tumor suppressor. We suggest that BARD1 is a mediator of apoptosis since (1) cell death in vivo (ischemic stroke) and in vitro is accompanied by increased levels of BARD1 protein and mRNA; (2) overexpression of BARD1 induces cell death with all features of ... More
FAM129B/MINERVA, a novel adherens junction-associated protein, suppresses apoptosis in HeLa cells.
AuthorsChen S, Evans HG, Evans DR,
JournalJ Biol Chem
PubMed ID21148485
A recent proteomics study identified FAM129B or MINERVA as a target of the MAP kinase (Erk1/2) signaling cascade in human melanoma cells. Phosphorylation of the protein was found to promote cell invasion and the dissociation of the protein from the cell-cell junctions. Suppression of apoptosis during metastasis is a prerequisite ... More
A role for hydrogen peroxide in the pro-apoptotic effects of photodynamic therapy.
AuthorsPrice M, Terlecky SR, Kessel D,
JournalPhotochem Photobiol
PubMed ID19659920
Although the first reactive oxygen species (ROS) formed during irradiation of photosensitized cells is almost invariably singlet molecular oxygen (1O2), other ROS have been implicated in the phototoxic effects of photodynamic therapy (PDT). Among these are superoxide anion radical (*O2(-)), hydrogen peroxide (H2O2) and hydroxyl radical (*OH). In this study, ... More
Nanoliter homogenous ultra-high throughput screening microarray for lead discoveries and IC50 profiling.
AuthorsMa H, Horiuchi KY, Wang Y, Kucharewicz SA, Diamond SL
JournalAssay Drug Dev Technol
PubMed ID15871692
Microfluidic technologies offer the potential for highly productive and low-cost ultra-high throughput screening and high throughput selectivity profiling. Such technologies need to provide the flexibility of plate-based assays as well as be less expensive to operate. Presented here is a unique microarray system (the Reaction Biology [Malvern, PA] DiscoveryDot), which ... More
Sphingosine 1-phosphate-induced cell proliferation, survival, and related signaling events mediated by G protein-coupled receptors Edg3 and Edg5.
AuthorsAn S, Zheng Y, Bleu T
JournalJ Biol Chem
PubMed ID10617617
Sphingosine 1-phosphate (S1P) regulates cell proliferation, apoptosis, motility, and neurite retraction. Contradictory reports propose that S1P acts as either an intracellular second messenger or an extracellular ligand for cell-surface receptors. Hence, the precise signaling mechanisms mediating the diverse cellular effects of S1P remain to be determined. Here, we investigate whether ... More
Staurosporine-induced apoptosis in human cornea epithelial cells in vitro.
AuthorsHärtel S, Zorn-Kruppa M, Tykhonova S, Alajuuma P, Engelke M, Diehl HA
JournalCytometry A
PubMed ID12938184
BACKGROUND: Apoptosis is a an important process in corneal development, homeostasis, and disease. This study was performed to determine for the first time basic temporal apoptotic features of SV-40 immortalized human corneal epithelial (HCE) cells. Additionally, we introduce a sensitive analysis of confocal microscopic images to measure the kinetics of ... More
Cellular apoptosis and cytotoxicity of phenolic compounds: a quantitative structure-activity relationship study.
AuthorsSelassie CD, Kapur S, Verma RP, Rosario M
JournalJ Med Chem
PubMed ID16279782
In this comprehensive study on the caspase-mediated apoptosis-inducing effect of 51 substituted phenols in a murine leukemia cell line (L1210), we determined the concentrations needed to induce caspase activity by 50% (I50) and utilized these data to develop the following quantitative structure-activity relationship (QSAR) model: log 1/I50 = 1.06 B5(2) ... More