Interaction of the Conserved Region 4.2 of sigma E with the RseA Anti-sigma Factor.
Authors Tam Christina; Collinet Bruno; Lau Gary; Raina Satish; Missiakas Dominique;
JournalJ Biol Chem
PubMed ID12016219
Evarsigma(E) RNA polymerase transcribes a regulon of folding factors for the bacterial envelope and is induced by physical and chemical stresses. The RseA anti-sigma factor inhibits the activity of Evarsigma(E) RNA polymerase. It is shown here that the N-terminal portion of varsigma(E), residues 1-153, binds core RNA polymerase. RseA interacts ... More
Heme oxygenase-2 is a hemoprotein and binds heme through heme regulatory motifs that are not involved in heme catalysis.
AuthorsMcCoubrey WK Jr, Huang TJ, Maines MD
JournalJ Biol Chem
PubMed ID9139709
The heme oxygenase (HO) system degrades heme to biliverdin and CO and releases chelated iron. In the primary sequence of the constitutive form, HO-2, there are three potential heme binding sites: two heme regulatory motifs (HRMs) with the absolutely conserved Cys-Pro pair, and a conserved 24-residue heme catalytic pocket with ... More
Identification of amino acid residues critical for LD78beta, a variant of human macrophage inflammatory protein-1alpha, binding to CCR5 and inhibition of R5 human immunodeficiency virus type 1 replication.
In an attempt to determine which amino acid(s) of LD78beta, a variant of human macrophage inflammatory protein-1alpha, plays a critical role in the interaction with CCR5, we generated six LD78beta variants with an amino acid substituted to Ala at the NH(2) terminus of LD78beta. There was no significant difference in ... More
Spinesin/TMPRSS5, a novel transmembrane serine protease, cloned from human spinal cord.
A cDNA encoding a novel serine protease, which we designated spinesin, has been cloned from human spinal cord. The longest open reading frame was 457 amino acids. A homology search revealed that the human spinesin gene was located at chromosome 11q23 and contained 13 exons, the gene structure being similar ... More