EnzChek™ 溶菌酶检测试剂盒
EnzChek™ 溶菌酶检测试剂盒
Invitrogen™

EnzChek™ 溶菌酶检测试剂盒

EnzChek™ 溶菌酶检测试剂盒为研究人员提供了一种简单灵敏的检测方法来测量溶液中的溶菌酶活性。利用荧光计或荧光微孔板读数仪测量溶菌酶活性产生的荧光强度增加。查看我们完整的荧光微孔板检测产品线。•检测到低至 20 U/mL 的溶菌酶活性浓度• 使用标准荧光素 (FITC)了解更多信息
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货号数量
E22013400 Assays
货号 E22013
价格(CNY)
5,256.00
Each
添加至购物车
数量:
400 Assays
价格(CNY)
5,256.00
Each
添加至购物车
EnzChek™ 溶菌酶检测试剂盒为研究人员提供了一种简单灵敏的检测方法来测量溶液中的溶菌酶活性。利用荧光计或荧光微孔板读数仪测量溶菌酶活性产生的荧光强度增加。

查看我们完整的荧光微孔板检测产品线

•检测到低至 20 U/mL 的溶菌酶活性浓度
• 使用标准荧光素 (FITC) 激发/发射设置
• 规格允许持续测量溶菌酶活性

该测定试剂盒利用一种创新性方法测量溶菌酶活性。使用的溶壁微球菌细胞壁已被荧光素广泛标记,从而导致荧光信号猝灭。活性溶菌酶可水解粘多糖细胞壁内 N-乙酰胞壁酸与 N-乙酰-D-葡糖胺残基之间的 b-(1-4)-糖苷键,从而消除猝灭并产生与溶菌酶活性成比例的显著荧光强度增加。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光强度
数量400 Assays
运输条件室温
底物属性脂基底物
底物类型溶菌酶底物
目标酶溶菌酶
适用于(应用)溶菌酶检测
适用于(设备)荧光微孔板读数仪
产品线EnzChek™
产品类型溶菌酶检测
Unit SizeEach
内容与储存
在冷冻冰箱(-5°C 至 -30°C)中避光储存。

常见问题解答 (FAQ)

How can I limit signal saturation when using the EnzChek Lysozyme Assay Kit (Cat. No. E22013)?

To limit signal saturation when using the EnzChek Lysozyme Assay Kit (Cat. No. E22013), try lowering the voltage (gain) on the PMT (photomultiplier tube). If you are still seeing signal saturation after lowering the voltage, try a shorter reaction time, and/or lower substrate concentration.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How was the substrate (Component A) in the EnzChek Lysozyme Assay Kit labeled?

The primary amines on the cell wall of Micrococcus lysodeikticus were conjugated to fluorescein.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

May other buffers be used for the EnzChek Lysozyme assay?

Yes. You may use other buffers that are compatible with the enzyme of interest. The buffer provided is optimal for use with chicken egg white lysozyme. If the buffer has a pH <6, adjust the pH to 7.5 to 8 prior to detection for optimal signal from the fluorescein. The emission of fluorescein drops off at pH <6.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.