Solution structural dynamics of HIV-1 reverse transcriptase heterodimer.
AuthorsSeckler JM, Howard KJ, Barkley MD, Wintrode PL,
JournalBiochemistry
PubMed ID19594135
'Crystal structures and simulations suggest that conformational changes are critical for the function of HIV-1 reverse transcriptase. The enzyme is an asymmetric heterodimer of two subunits, p66 and p51. The two subunits have the same N-terminal sequence, with the p51 subunit lacking the C-terminal RNase H domain. We used hydrogen ... More
Apobec3 encodes Rfv3, a gene influencing neutralizing antibody control of retrovirus infection.
Recovery from Friend virus 3 (Rfv3) is a single autosomal gene encoding a resistance trait that influences retroviral neutralizing antibody responses and viremia. Despite extensive research for 30 years, the molecular identity of Rfv3 has remained elusive. Here, we demonstrate that Rfv3 is encoded by Apobec3. Apobec3 maps to the ... More
Importance of SARS-CoV spike protein Trp-rich region in viral infectivity.
AuthorsLu Y, Neo TL, Liu DX, Tam JP,
JournalBiochem Biophys Res Commun
PubMed ID18424264
SARS-CoV entry is mediated by spike glycoprotein. During the viral and host cellular membrane fusion, HR1 and HR2 form 6-helix bundle, positioning the fusion peptide closely to the C-terminal region of ectodomain to drive apposition and subsequent membrane fusion. Connecting to the HR2 region is a Trp-rich region which is ... More
Evolution of subtype C HIV-1 Env in a slowly progressing Zambian infant.
AuthorsZhang H, Hoffmann F, He J, He X, Kankasa C, Ruprecht R, West JT, Orti G, Wood C,
JournalRetrovirology
PubMed ID16274482
BACKGROUND: Given the high prevalence of mother to child infection, the development of a better understanding of African subtype C HIV-1 transmission and natural evolution is of significant importance. In this study, we genotypically and phenotypically characterized subtype C viruses isolated over a 67-month follow-up period from an in utero-infected ... More