Fluorescence energy transfers between points in acto-subfragment-1 rigor complex.
AuthorsMiki M, Wahl P
JournalBiochim Biophys Acta
PubMed ID6487641
Fluorescence energy transfer was measured by time-resolved and steady-state fluorimetry in order to investigate the spatial relationships between the nucleotide binding site of actin, the Cys-373 residue of actin, and the SH1 of myosin subfragment-1 in the rigor complex of acto-subfragment-1. N-Iodoacetyl-N'-(5-sulfo-1-naphthyl)ethylenediamine (IAEDANS) bound to the Cys-373 of actin or ... More
Fluorescence resonance energy transfer between sites in G-actin. The spatial relationship between Cys-10, Tyr-69, Cys-374, the high-affinity metal and the nucleotide.
AuthorsBarden JA, dos Remedios CG
JournalEur J Biochem
PubMed ID3665911
'Intramonomer fluorescence resonance energy transfer spectroscopy was employed to investigate the spatial relationship between labels attached to the residues Cys-10, Tyr-69, Cys-374, the high-affinity metal binding site and the nucleotide binding site in G-actin. The separation between the fluorescence donor 5-(dimethylamino)naphthalene-1-sulphonyl (Dns) chloride (dansyl chloride) used to label Tyr-69 and ... More
Differential behavior of two cysteine residues on the myosin head in muscle fibers.
AuthorsMiyanishi T, Borejdo J
JournalBiochemistry
PubMed ID2523734
'We have previously shown that the orientation of (iodoacetamido)tetramethylrhodamine labels on SH1 thiol of S-1 moieties changes when MgADP is added to the fibers in rigor [Borejdo, J., Assulin, O., Ando, T., & Putnam, S. (1982) J. Mol. Biol. 158, 391-414. Burghardt, T.P., Ando, T., & Borejdo, J. (1983) Proc. ... More
Nucleotide-free actin: stabilization by sucrose and nucleotide binding kinetics.
AuthorsDe La Cruz EM, Pollard TD
JournalBiochemistry
PubMed ID7727403
'We prepared nucleotide-free actin in buffer containing 48% (w/v) sucrose. Sucrose inhibits the irreversible denaturation of actin that follows nucleotide dissociation [Kasai et al. (1965) Biochim. Biophys. Acta 94, 494-503]. Our conditions removed nucleotide from approximately 80% of the actin. Stabilization of nucleotide-free actin depends on the sucrose concentration. The ... More
Structural connectivity in actin: effect of C-terminal modifications on the properties of actin.
AuthorsCrosbie RH, Miller C, Cheung P, Goodnight T, Muhlrad A, Reisler E
JournalBiophys J
PubMed ID7858132
'In this study, we use fluorescent probes and proteolytic digestions to demonstrate structural coupling between distant regions of actin. We show that modifications of Cys-374 in the C-terminus of actin slow the rate of nucleotide exchange in the nucleotide cleft. Conformational coupling between the C-terminus and the DNasal loop in ... More
Opening of the myosin nucleotide triphosphate binding domain during the ATPase cycle.
AuthorsPate E, Naber N, Matuska M, Franks-Skiba K, Cooke R
JournalBiochemistry
PubMed ID9315852
'A series of ATP analogs, in which moieties of various sizes have been added to the gamma-phosphorus of ATP, bind to the active site of myosin and to the actomyosin complex in myofibrils and in chemically skinned fibers. The affinity of the analogs for the active site shows only a ... More
Characterization of the properties of ethenoadenosine nucleotides bound or trapped at the active site of myosin subfragment 1.
AuthorsPerkins WJ, Wells JA, Yount RG
JournalBiochemistry
PubMed ID6237680
'The fluorescent nucleotide analogue of ADP, 1,N6-ethenoadenosine diphosphate (epsilon ADP), has been used to probe the active site of myosin subfragment 1 (SF1). The Mg complex of ADP was shown to be trapped stoichiometrically at the active site by a variety of thiol cross-linking agents having sulfur to sulfur spanning ... More
Comparison of contractions of the smooth muscle of the guinea-pig vas deferens induced by ATP and related nucleotides.
'The shape of contractile responses of the isolated guinea-pig vas deferens changes as ATP concentration is increased from 10(-7) to 10(-2) M. The ATP concentration-response curve is bimodal and reflects the change in response profile. Initially spike-like (10(-7) -3 x 10(-5) M) in nature, contractions acquire a secondary, slower tonic ... More
Antagonistic effects of cofilin, beryllium fluoride complex, and phalloidin on subdomain 2 and nucleotide-binding cleft in F-actin.
AuthorsMuhlrad A, Ringel I, Pavlov D, Peyser YM, Reisler E
JournalBiophys J
PubMed ID16997870
'Cofilin/ADF, beryllium fluoride complex (BeFx), and phalloidin have opposing effects on actin filament structure and dynamics. Cofilin/ADF decreases the stability of F-actin by enhancing disorder in subdomain 2, and by severing and accelerating the depolymerization of the filament. BeFx and phalloidin stabilize the subdomain 2 structure and decrease the critical ... More
The kinetics of effector binding to phosphofructokinase. The influence of effectors on the allosteric conformational transition.
AuthorsRoberts D, Kellett GL
JournalBiochem J
PubMed ID6260084
'1. The extent of the allosteric transition from the R into the T conformation of rabbit skeletal muscle phosphofructokinase induced by Mg2+-1,N6-etheno-ATP was determined by stopped-flow fluorimetry from the amplitude of the slow phase of the Mg2+-1,N6-etheno-ATP fluorescence enhancement [Roberts & Kellet (1979) Biochem. J. 183, 349--360]. 2. The amplitude ... More
The mechanism of regulation of actomyosin subfragment 1 ATPase.
AuthorsRosenfeld SS, Taylor EW
JournalJ Biol Chem
PubMed ID2956257
'The mechanism of regulation of actin-subfragment 1 nucleoside triphosphatase is described in terms of the rate and equilibrium constants of a relatively simple kinetic scheme: (Formula: see text) where T, D, and Pi are nucleoside triphosphate, nucleoside diphosphate, and inorganic phosphate, respectively; Ka, Kb, and Kc are association constants; the ... More
Interdependence of profilin, cation, and nucleotide binding to vertebrate non-muscle actin.
AuthorsKinosian HJ, Selden LA, Gershman LC, Estes JE
JournalBiochemistry
PubMed ID11052670
'The interaction of profilin and non-muscle beta,gamma-actin prepared from bovine spleen has been investigated under physiologic ionic conditions. Profilin binding to actin decreases the affinity of actin for MgADP and MgATP by about 65- and 13-fold, respectively. Kinetic measurements indicate that profilin binding to actin weakens the affinity of actin ... More
Exchange of 1,N6-etheno-ATP with actin-bound nucleotides as a tool for studying the steady-state exchange of subunits in F-actin solutions.
AuthorsWang YL, Taylor DL
JournalProc Natl Acad Sci U S A
PubMed ID6946487
'The fluorescent analog of ATP 1-N6-ethenoadenosine 5''-triphosphate (epsilon-ATP) exchanges readily with nucleotides bound to G-actin. The exchange can be observed by measuring the fluorescence intensity, which increases significantly when epsilon-ATP binds to actin. When excess epsilon-ATP is added to a solution of F-actin, a continuous increase in fluorescence intensity is ... More
Actobindin induces the accumulation of actin dimers that neither nucleate polymerization nor self-associate.
AuthorsBubb MR, Knutson JR, Porter DK, Korn ED
JournalJ Biol Chem
PubMed ID7929262
'Actobindin purified from Acanthamoeba castellanii inhibits the nucleation, but not the elongation, phase of actin polymerization. Previously, we had speculated that actobindin, which can simultaneously bind two actin monomers (Bubb, M.R., Lewis, M.S., and Korn, E.D. (1991) J. Biol. Chem. 266, 3820-3826), might preferentially interact with small oligomers and inhibit ... More
Localization of the phalloidin and nucleotide-binding sites on actin.
AuthorsBarden JA, Miki M, Hambly BD, Dos Remedios CG
JournalEur J Biochem
PubMed ID3830158
'Phalloidin was found to block nucleotide exchange in F-actin, without interfering with nucleotide hydrolysis. This inhibition of nucleotide exchange occurs under conditions in which monomers are able to exchange. The distance separating a fluorescent chromophore attached to phalloidin from the nucleotide on actin was determined using fluorescence resonance energy-transfer spectroscopy. ... More
Distance between nucleotide site and cysteine-373 of G-actin by resonance energy transfer measurements.
AuthorsCheung HC, Liu BM
JournalJ Muscle Res Cell Motil
PubMed ID6715528
'The distance between the nucleotide site and the reactive cysteine-373 of G-actin was determined from resonance energy transfer measurements by using 1,N6-ethenoadenosine triphosphate (epsilon ATP) as the donor and 4-[N-(iodoacetoxy)ethyl N methyl]amino 7 nitrobenz 2 oxa 1,3 diazole covalently attached to the sulphydryl group as acceptor. The quenching of the ... More
Mechanism for nucleotide exchange in monomeric actin.
AuthorsFrieden C, Patane K
JournalBiochemistry
PubMed ID3408729
'Rabbit skeletal muscle G-actin has been treated to obtain ADP, 1,N6-ethenoadenosine diphosphate (epsilon-ADP), or 1,N6-ethenoadenosine triphosphate (epsilon-ATP) at the nucleotide binding site and either Mg2+ or Ca2+ at high- and moderate-affinity metal binding sites. Apparent rates or rate constants for the displacement of the actin-bound nucleotides by epsilon-ATP or ATP ... More
Cofactor-induced orientation of the DNA bases in single-stranded DNA complexed with RecA protein. A fluorescence anisotropy and time-decay study.
AuthorsChabbert M, Lami H, Takahashi M
JournalJ Biol Chem
PubMed ID2005086
'The structure of the RecA-single-stranded DNA complex was investigated by studying the fluorescence emission of poly(deoxy-1,N6-ethenoadenylic acid (poly(d epsilon A)), a fluorescent derivative of poly(dA), under various viscosity conditions. The fluorescence intensity and average lifetime of poly(d epsilon A) are much smaller than those of nonpolymerized monoethenonucleotides (1,N6-ethenoadenosine 5''-triphosphate and ... More
A biosensor for fluorescent determination of ADP with high time resolution.
AuthorsKunzelmann S, Webb MR,
JournalJ Biol Chem
PubMed ID19801632
'Nearly every cellular process requires the presence of ATP. This is reflected in the vast number of enzymes like kinases or ATP hydrolases, both of which cleave the terminal phosphate from ATP, thereby releasing ADP. Despite the fact that ATP hydrolysis is one of the most fundamental reactions in biological ... More
Charge effects on the dynamic quenching of fluorescence of 1,N6-ethenoadenosine oligophosphates by iodide, thallium (I) and acrylamide.
AuthorsAndo T, Asai H
JournalJ Biochem (Tokyo)
PubMed ID7410337
'Systematic studies were carried out to determine how the dynamic quenching of fluorescence was affected by electrostatic interaction between charges carried by the fluorophore and the quencher. 1,N6-Ethenoadenosine oligophosphates (epsilon-ATP, epsilon-ADP, epsilon-AMP, and epsilon-Ad) were used as fluorophores; these compounds have the same luminous group (epsilon-adenine ring) with variously charged ... More
'Hsp90 is one of the most abundant proteins in the cytosol of eukaryotic cells. Under physiological conditions Hsp90 has been shown to play a major role in several specific signaling pathways, including maturation of various kinases and maintenance of steroid receptors in an activable state. It is well established that ... More
Metal dependence and thermodynamic characteristics of the beef heart mitochondrial adenosine triphosphatase.
AuthorsDorgan LJ, Urbauer JL, Schuster SM
JournalJ Biol Chem
PubMed ID6230351
'A systematic study was done examining the steady state kinetics of F1-catalyzed nucleotide hydrolysis in the presence of various activating divalent metal cations. Values of Km and kcat were obtained from Lineweaver-Burk plots, and kcat/Km values were calculated. With some exceptions, kcat/Km was shown to be independent of the metal ... More
Control of actin filament length and turnover by actin depolymerizing factor (ADF/cofilin) in the presence of capping proteins and ARP2/3 complex.
'The effect of Arabidopsis thaliana ADF1 and human ADF on the number of filaments in F-actin solutions has been examined using a seeded polymerization assay. ADF did not sever filaments in a catalytic fashion, but decreased the steady-state length distribution of actin filaments in correlation with its effect on actin ... More
Fluorescence resonance energy transfer between the nucleotide binding site and Cys-10 in G-actin and F-actin.
AuthorsMiki M, Barden JA, dos Remedios CG
JournalBiochim Biophys Acta
PubMed ID3089284
'Intramonomer fluorescence resonance energy transfer between the donor epsilon-ATP bound to the nucleotide site and the acceptor N-(4-dimethylamino-3,5-dinitrophenyl)maleimide (DDPM) or 4-dimethylaminophenyl-azophenyl-4''-maleimide bound to Cys-10 in G-actin was measured. The donor-acceptor distance was calculated to be about 40 A. The intermonomer energy transfer in F-actin occurring between epsilon-ADP and DABMI was ... More
Actin's view of actomyosin interface.
AuthorsMiller CJ, Cheung P, White P, Reisler E
JournalBiophys J
PubMed ID7787100
'Actomyosin interactions were examined by using yeast actin mutants with alanines replacing charged amino acid pairs D24/D25, E99/E100, D80/D81, and E83/K84. In the in vitro motility experiments, actin filaments of D24A/D25A or E99A/E100A mutants moved in the presence of 0.7% methylcellulose at the velocities of wild-type actin. Without methylcellulose, these ... More
Effects of subtilisin cleavage of monomeric actin on its nucleotide binding.
AuthorsOoi A, Mihashi K
JournalJ Biochem (Tokyo)
PubMed ID9010757
'The kinetics of ATP exchange on subtilisin-cleaved G-actin was investigated by measuring the fluorescence of 1,N6-ethenoadenosine 5''-triphosphate. The apparent dissociation rate of ATP (k-ATP) was 2.8-fold larger than that of intact G-actin in the presence of 300 microM free Ca2+. Analysis of the dependence of k-ATP on free Ca2+ showed ... More
Selective binding and uptake of ribonuclease A and glyceraldehyde-3-phosphate dehydrogenase by isolated rat liver lysosomes.
AuthorsCuervo AM, Terlecky SR, Dice JF, Knecht E
JournalJ Biol Chem
PubMed ID7929357
'Ribonuclease A (RNase A) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) are selectively taken up and degraded by isolated rat liver lysosomes by very similar processes. The uptake and degradation of both of these proteins are stimulated by the heat shock cognate protein of 73 kDa and ATP/Mg2+. Both binding and uptake of ... More
Nucleotide vesicular transporter of bovine chromaffin granules. Evidence for a mnemonic regulation.
AuthorsGualix J, Abal M, Pintor J, Garcia-Carmona F, Miras-Portugal MT
JournalJ Biol Chem
PubMed ID8567644
'The nucleotide vesicular transport has been studied with the fluorescent substrate analogues, the (1,N6-ethenoadenosine) nucleotides. The transport experiments were carried out with granular preparations from bovine adrenal medulla, and epsilon-ATP, epsilon-ADP, and epsilon-AMP were quantified after separation by high performance liquid chromatography. The granular concentration increase of all three nucleotides ... More
Cryoenzymic studies on actomyosin ATPase. Evidence that the degree of saturation of actin with myosin subfragment 1 affects the kinetics of the binding of ATP.
AuthorsTesi C, Travers F, Barman T
JournalBiochemistry
PubMed ID2139580
'The initial steps of actomyosin subfragment 1 (acto-S1) ATPase (dissociation and binding of ATP) were studied at -15 degrees C with 40% ethylene glycol as antifreeze. The dissociation kinetics were followed by light scattering in a stopped-flow apparatus, and the binding of ATP was followed by the ATP chase method ... More
Binding of divalent cation and nucleotide to G-actin in the presence of profilin.
AuthorsPerelroizen I, Carlier MF, Pantaloni D
JournalJ Biol Chem
PubMed ID7829477
'The effect of profilin, a G-actin binding protein, on the mechanism of exchange of the tightly bound metal ion and nucleotide on G-actin, has been investigated. 1) In low ionic strength buffer, profilin increases the rates of Ca2+ and Mg2+ dissociation from G-actin 250- and 50-fold, respectively. On the profilin-actin ... More
The activity of -adenosine derivatives as allosteric modifiers of phosphorylase b.
AuthorsSteiner RF
JournalFEBS Lett
PubMed ID4673706
Fluorescent modification of adenosine-containing coenzymes. Biological activities and spectroscopic properties.
AuthorsSecrist JA, Barrio JR, Leonard NJ, Weber G
JournalBiochemistry
PubMed ID4340904
High molecular weight precursor polypeptides to structural proteins of Rauscher murine leukemia virus.
AuthorsShapiro SZ, Strand M, August JT
JournalJ Mol Biol
PubMed ID63555
Effects of growth hormone on ATPase and fluorescence of isolated liver membranes utilizing the fluorescent substrate, 1,N6-etheno-adenosine triphosphate.
AuthorsAizono Y, Roberts JE, Sonenberg M, Swislocki NI
JournalArch Biochem Biophys
PubMed ID4278079
Similar affinities of ADP and ATP for G-actin at physiological salt concentrations.
AuthorsWanger M, Wegner A
JournalFEBS Lett
PubMed ID6617883
'The equilibrium constant for the exchange of ATP and ADP at G-actin was determined by fluorimetric titration of G-actin-bound epsilon-ATP by ATP or ADP. The affinity of ATP for G-actin was found to be only about 3-fold higher than the affinity of ADP for G-actin at 37 degrees C, pH ... More
Binding of 1,N6-ethanoadenosine triphosphate to actin.
AuthorsThames KE, Cheung HC, Harvey SC
JournalBiochem Biophys Res Commun
PubMed ID4214008
Fluorescence studies of phosphoribosyladenosine triphosphate synthetase of Escherichia coli.
AuthorsTébar AR, Fernández VM, Martíndelrío R, Ballesteros AO
JournalFEBS Lett
PubMed ID1089566
Bovine plasma high molecular weight kininogen: the amino acid sequence of fragment 1 (glycopeptide) released by the action of plasma kallikrein and its location in the precursor protein.
AuthorsHan YN, Kato H, Iwanaga S, Suzuki T
JournalFEBS Lett
PubMed ID1261681
A fluorescent modification of adenosine triphosphate with activity in enzyme systems: 1,N 6 -ethenoadenosine triphosphate.
AuthorsSecrist JA, Barrio JR, Leonard NJ
JournalScience
PubMed ID4257930
'A new, highly fluorescent adenosine triphophate (ATP) analog, 1,N(6) ethenoadenosine triphosphate, has been synthesized. Its fluorescence properties, including the long fluorescence lifetime and the possibility of detection at very low concentrations, in conjunction with its activity in the representative enzyme systems here reported, make it a valuable probe of enzymic ... More
Exchange of ADP, ATP and 1: N6-ethenoadenosine 5'-triphosphate at G-actin. Equilibrium and kinetics.
AuthorsNeidl C, Engel J
JournalEur J Biochem
PubMed ID510301
A new principle for activity measurement of ADP or ATP dependent enzymes: fluorescence quenching of epsilon-ADP and epsilon-ATP by divalent metal ions.
AuthorsHöhne WE, Heitmann P
JournalAnal Biochem
PubMed ID3123
Pyruvate as a fluorescence quencher: a new spectroscopic assay for pyruvate reactions.
AuthorsAndo T, Miyata H
JournalAnal Biochem
PubMed ID6222667
'Pyruvate ion, which is biologically ubiquitous and participates in many metabolic reactions, was found to be an effective quencher of fluorescence. Compared to other negatively charged quenchers such as I-, pyruvate is not toxic to proteins. By adding an inert, long-lived fluorophore to systems transacting pyruvate, it is possible to ... More
Intramolecular distances within the Ca(2+)-ATPase from sarcoplasmic reticulum as estimated through fluorescence energy transfer between probes.
'Fluorescence energy transfer measurements have been carried out to estimate intramolecular distances between probes bound to Ca(2+)-transporting ATPase (Ca(2+)-ATPase) as well as distances between these probes and the phospholipid headgroup. The nucleotide binding site was monitored by using 1,N6-ethenoadenosine 5''-triphosphate, a fluorescent analogue of ATP, and also by labelling Lys515 ... More
Kinetic and binding effects of 1,N 6 -ethenoadenosine triphosphate to aspartate transcarbamylase.
AuthorsChien Y, Weber G
JournalBiochem Biophys Res Commun
PubMed ID4569879
Random copolymerization of ATP-actin and ADP-actin.
AuthorsOhm T, Wegner A
JournalBiochemistry
PubMed ID1958656
'The equilibrium of the copolymerization of ATP-actin and ADP-actin was investigated by an analysis of the critical concentrations of mixtures of ATP-actin and ADP-actin. The molar ratio of bound ATP to bound ADP was controlled by the ratio of free ATP and ADP. The experiments were performed under conditions (100 ... More
Quenching of fluorescent nucleotides bound to myosin: a probe of the active-site conformation.
AuthorsFranks-Skiba K, Hwang T, Cooke R
JournalBiochemistry
PubMed ID7918498
'The conformation of the active ATPase site of myosin subfragment 1 (S1) and actomyosin in myofibrils was probed by measuring the solvent accessibility of the bound ethenonucleotides epsilon ADP and epsilon ATP (during steady-state hydrolysis). Solvent accessibility was determined by measuring the quenching of fluorescence produced by the solvent-phase quencher ... More
Reversible inactivation of myosin subfragment 1 activity by mechanical immobilization.
AuthorsHighsmith S, Duignan K, Franks-Skiba K, Polosukhina K, Cooke R
JournalBiophys J
PubMed ID9512042
'The Mg-ATPase activity of skeletal muscle myosin subfragment 1 (S1) is reversibly eliminated when it is aggregated by the force of osmotic pressure dehydration using polyethylene glycol (PEG). Several experiments indicate nucleotides bind aggregated S1, but the effects of binding are attenuated. Compared with S1 in solution, epsilonADP binds aggregated ... More
S-Adenosylmethionine synthetase from Escherichia coli.
AuthorsMarkham GD, Hafner EW, Tabor CW, Tabor H
JournalJ Biol Chem
PubMed ID6251075
'Adenosylmethionine (AdoMet) synthetase has been purified to homogeneity from Escherichia coli. For this purification, a strain of E. coli which was derepressed for AdoMet synthetase and which harbors a plasmid containing the structural gene for AdoMet synthetase was constructed. This strain produces 80-fold more AdoMet synthetase than a wild type ... More
The effects of severely decreased hydrophobicity in a subdomain 3/4 loop on the dynamics and stability of yeast G-actin.
AuthorsKuang B, Rubenstein PA
JournalJ Biol Chem
PubMed ID9020164
'The hydrophobicity of the subdomain 3/4 hydrophobic loop (262-274) has been implicated to be essential for actin''s function. We previously showed (Kuang, B., and Rubenstein, P. A. (1997) J. Biol. Chem. 272, 1237-1247) that a mutant yeast actin (V266G/L267G) with markedly decreased hydrophobicity in this loop conferred severe cold sensitivity ... More
The effect of nitration and D2O on the kinetics of beef heart mitochondrial adenosine triphosphatase.
AuthorsDorgan LJ, Schuster SM
JournalJ Biol Chem
PubMed ID6452457
'The role of tyrosine in the catalytic mechanism of nucleoside triphosphate hydrolysis by beef heart mitochondrial ATPase is explored. We compare the rates of the ATPase reaction by both nitrated and native F1 at both pH 8 and pH 6. The pH-activity profile of nitrated F1 is compared to the ... More
Synergy between actin depolymerizing factor/cofilin and profilin in increasing actin filament turnover.
AuthorsDidry D, Carlier MF, Pantaloni D
JournalJ Biol Chem
PubMed ID9748225
'The mechanism of control of the steady state of actin assembly by actin depolymerizing factor (ADF)/cofilin and profilin has been investigated. Using Tbeta4 as an indicator of the concentration of ATP-G-actin, we show that ADF increases the concentration of ATP-G-actin at steady state. The measured higher concentration of ATP-G-actin is ... More
Selective determination of adenine-containing compounds by capillary electrophoresis with laser-induced fluorescence detection.
AuthorsTseng HC, Dadoo R, Zare RN
JournalAnal Biochem
PubMed ID7856871
'Capillary electrophoresis coupled with laser-induced fluorescence detection provides a selective analysis of mixtures of adenine-containing compounds (adenine, adenosine, cAMP, AMP, ADP, and ATP) that are derivatized using chloroacetaldehyde as a fluorogenic reagent. The components can be detected with linear response over the concentration range of 10(-4) to 10(-9) M, and ... More
Opposite effects of cofilin and profilin from porcine brain on rate of exchange of actin-bound adenosine 5'-triphosphate.
AuthorsNishida E
JournalBiochemistry
PubMed ID4096896
'Cofilin, an actin-binding protein isolated from porcine brain that reacts with actin in a 1:1 molar ratio [Nishida, E., Maekawa, S., & Sakai, H. (1984) Biochemistry 23, 5307-5313], decreases the rate of exchange of ATP bound to G-actin with 1,N6-ethenoadenosine 5''-triphosphate in solution. From analyses of the dependence of the ... More
Classification of nucleotide binding sites on mitochondrial F1-ATPase from yeast.
AuthorsRecktenwald D, Hess B
JournalBiochim Biophys Acta
PubMed ID6448067
'Methods are described to classify nucleotide binding sites of the mitochondrial coupling factor F1 from yeast on the basis of their affinities and stability properties. High affinity sites or states for ATP and related adenine analogs and low affinity sites or states which bind a broad range of different nucleotide ... More
The extent of mitochondrial F1-ATPase and adenine nucleotide carrier activity with epsilon-ATP.
AuthorsKaplan RS, Coleman PS
JournalBiochim Biophys Acta
PubMed ID145875
'1. The use of 1,N6-ethenoadenosine 5''-triphosphate (epsilon-ATP), a synthetic, fluorescent analog of ATP, by whole rat liver mitochondria and by submitochondrial particles produced via sonication has been studied. 2. Direct [3H]adenine nucleotide uptake studies with isolated mitochondria, indicate the epsilon-[3H]ATP is not transported through the inner membrane by the adenine ... More
The kinetics of the exchange of G-actin-bound 1: N6-ethenoadenosine 5'-triphosphate with ATP as followed by fluorescence.
AuthorsWaechter F, Engel J
JournalEur J Biochem
PubMed ID240724
'1: N6-Ethenoadenosine 5''-triphosphate (epsilonATP), a fluorescent analog of ATP, binds to monomeric actin with a binding constant which is only about 5 times smaller than that of ATP. The spectroscopic changes which occur when epsilonATP binds to actin are studied and used to monitor the kinetics of nucleotide exchange. The ... More
Transient kinetics of the interaction of 1,N6-ethenoadenosine 5'-triphosphate with myosin subfragment 1 under normal and cryoenzymic conditions: a comparison with adenosine 5'-triphosphate.
AuthorsTesi C, Travers F, Barman T
JournalBiochemistry
PubMed ID3167019
'The kinetics of the interaction of the fluorescent analogue 1,N6-ethenoadenosine 5''-triphosphate (epsilon-ATP) with myosin subfragment 1 (S1) were studied at 15 and -7.5 degrees C with 40% ethylene glycol as cryosolvent. Two techniques were used: fluorescence stopped flow and rapid flow-quench. When S1 is mixed with epsilon-ATP in a stopped-flow ... More
Cofactor A is a molecular chaperone required for beta-tubulin folding: functional and structural characterization.
AuthorsMelki R, Rommelaere H, Leguy R, Vandekerckhove J, Ampe C
JournalBiochemistry
PubMed ID8756698
'Actin and tubulin polypeptide chains acquire their native conformation in the presence of the chaperonin containing TCP-1 (CCT) and, in the case of alpha- and beta-tubulin additional protein cofactors. We recently identified one of these cofactors, termed cofactor A, that is required for the proper folding of the beta-tubulin chain ... More
Enzymatic incorporation of ATP and CTP analogues into the 3' end of tRNA.
AuthorsSprinzl M, Sternbach H, von der Haar F, Cramer F
JournalEur J Biochem
PubMed ID598381
'Structural analogues of adenosine 5''-triphosphate and cytidine 5''-triphosphate were investigated as substrates for ATP(CTP):tRNA nucleotidyl transferase. Eight out of 26 ATP analogues and six out of nine CTP analogues were incorporated into the 3'' terminus of tRNA. In general, for the recognition of the substrates the modification of the cytidine ... More
Kinetic properties of phenylalanyl-tRNA and seryl-tRNA synthetases for normal substrates and fluorescent analogs.
AuthorsHertz HS, Zachau HG
JournalEur J Biochem
PubMed ID4583343
Energy transfer from tryptophan residues to a fluorescent ATP analog, 1,N6-ethenoadenosine triphosphate, bound to H-meromyosin.
AuthorsOnishi H, Otsuka E, Ikehara M, Tonomura Y
JournalJ Biochem (Tokyo)
PubMed ID4585706
Nanosecond pulsefluorometry in polarized light of G-actin-epsilon-ATP and F-actin-epsilon-ADP.
AuthorsMihashi K, Wahl P
JournalFEBS Lett
PubMed ID1168157
The influence of Ca2 on the dissociation of 1,N6-ethenoadenosine 5'-triphosphate from actin.
AuthorsWaechter F
JournalHoppe Seylers Z Physiol Chem
PubMed ID1205454
Interaction of adenine nucleotides with the coupling factor of spinach chloroplasts. A hydrogen-deuterium exchange study.
AuthorsNabedryk-Viala E, Calvet P, Thiéry JM, Galmiche JM, Girault G
JournalFEBS Lett
PubMed ID891922
Circular polarization of the fluorescence of actin-bound epsilon ATP Effects of binding DNase I.
AuthorsBurtnick LD, Schaar PL
JournalFEBS Lett
PubMed ID761614
Effect of nucleotides and other inhibitors on the inactivation of glutamate decarboxylase.
AuthorsMartin DL, Martin SB
JournalJ Neurochem
PubMed ID7119776
Investigations into the efficacy of 1,N6-ethenoadenosine triphosphate as a substrate for contractility studies.
AuthorsMowery PC
JournalArch Biochem Biophys
PubMed ID4274085
Enzymatic studies on the interaction of myosin and heavy meromyosin with 1,N 6 -ethenoadenosine triphosphate ( ATP), a fluorescent analog of ATP.
AuthorsMcCubbin WD, Willick GE, Kay CM
JournalBiochem Biophys Res Commun
PubMed ID4265978
Interaction of photoreactive and fluorescent nucleotides with chloroplast coupling factor 1.
AuthorsCarlier MF, Holowka DA, Hammes GG
JournalBiochemistry
PubMed ID157773
Pre-steady state kinetics of nucleotide-triphosphate hydrolysis by mitochondrial F1-ATPase from yeast.
AuthorsRecktenwald D, Hess B
JournalFEBS Lett
PubMed ID160334
Conversion of the Ca2+-ATPase from rhodospirillum rubrum into a Mg2+-dependent enzyme by 1,N6-etheno ATP.
AuthorsSchäfer HJ, Müller HW, Dose K
JournalBiochem Biophys Res Commun
PubMed ID6448051
Interactions of phosphate ligands with Escherichia coli aspartate carbamoyltransferase in the crystalline state.
AuthorsHonzatko RB, Lipscomb WN
JournalJ Mol Biol
PubMed ID6294306
Affinity labeling of a guanosine 5'-triphosphate site of glutamate dehydrogenase by a fluorescent nucleotide analogue, 5'-[p-(fluorosulfonyl)benzoyl]-1,N6-ethenoadenosine.
AuthorsJacobson MA, Colman RF
JournalBiochemistry
PubMed ID7093238
Adenine binding sites of F1-ATPase are located on the surface of the protein molecule.
AuthorsKozlov IA, Novikova IYu
JournalFEBS Lett
PubMed ID6219000
Spectroscopic isolation of ES complexes of myosin subfragment-1 ATPase by fluorescence quenching.
AuthorsAndo T, Duke JA, Tonomura Y, Morales MF
JournalBiochem Biophys Res Commun
PubMed ID6218804
Fluorescence stopped-flow study of the mechanism of nucleotide binding to myosin subfragment I.
AuthorsGarland F, Cheung HC
JournalBiochemistry
PubMed ID518834
Studies on adenosine triphosphate transphosphorylases. XIII. Kinetic properties of the crystalline rabbit muscle ATP-AMP transphorphorylase (adenylate kinase) and a comparison with the crystalline calf muscle and liver adenylate kinases.
AuthorsHamada M, Kuby SA
JournalArch Biochem Biophys
PubMed ID214039
Binding of modified adenine nucleotides to isolated coupling factor from chloroplasts as measured by polarization of fluorescence.
AuthorsVandermeulen DL, Govindjee
JournalEur J Biochem
PubMed ID144054
Fluorescent nucleotide analogs: synthesis and applications.
AuthorsJameson DM, Eccleston JF
JournalMethods Enzymol
PubMed ID9170323
Fluorescence as a probe of contractile systems.
AuthorsCooke R
JournalMethods Enzymol
PubMed ID7121286
Etheno-substituted nucleotides and coenzymes: fluorescence and biological activity.
AuthorsLeonard NJ
JournalCRC Crit Rev Biochem
PubMed ID6365449
Species responsible for the fluorescence of 1:N6-ethenoadenosine.
AuthorsSpencer RD, Weber G, Tolman GL, Barrio JR, Leonard NJ
JournalEur J Biochem
PubMed ID4137201
Structure of actin observed by fluorescence resonance energy transfer spectroscopy.
AuthorsMiki M, O'Donoghue SI, Dos Remedios CG
JournalJ Muscle Res Cell Motil
PubMed ID1534564
Studies of adenosine triphosphate transphosphorylases. XIV. Equilibrium binding properties of the crystalline rabbit and calf muscle ATP--AMP transphosphorylase (adenylate kinase) and derived peptide fragments.
AuthorsHamada M, Palmieri RH, Russell GA, Kuby SA
JournalArch Biochem Biophys
PubMed ID224811
Interactions of fluorescent analogs of adenine nucleotides with coupling factor protein isolated from spinach chloroplasts.
AuthorsVandermeulen DL, Govindjee
JournalFEBS Lett
PubMed ID1181200
Firefly luciferase-luciferin-ATP mixtures respond to etheno-ATP with an increased and steady production of light: a partial explanation of the Limulus ventral photoreceptor controversy.
AuthorsFord SR, Leach FR
JournalAm J Physiol
PubMed ID1767822
Nucleotide trapping at the ATPase site of myosin subfragment 1 by a new interthiol crosslinking.
AuthorsChaussepied P, Mornet D, Kassab R
JournalProc Natl Acad Sci U S A
PubMed ID2938184
When myosin subfragment 1 derivatives in which the reactive sulfhydryl SH1 has been blocked react with N,N'-p-phenylenedimaleimide or 5,5'-dithiobis(2-nitrobenzoic acid), the reactive sulfhydryl group SH2 of the 20-kDa domain is crosslinked with a thiol of the 50-kDa domain of the heavy chain. The crosslink induces the stable trapping of a ... More
Fluorescence energy transfer between epsilon-ATP at the nucleotide binding site and N-(4-dimethylamino-3,5-dinitrophenyl)-maleimide at Cys-373 of G-actin.
AuthorsMiki M, Mihashi K
JournalBiochim Biophys Acta
PubMed ID638187
The method of fluorescence energy transfer is used to measure the distance from the nucleotide binding site to Cys-373 of G-actin. The fluorescent ATP analogue 1-N6-ethenoadenosine 5'-triphosphate was used as donor and N-(4-dimethylamino-3,5-dinitrophenyl)-maleimide was used as acceptor. From the measurements of the efficiency of fluorescence energy transfer by both static ... More
The interaction of bovine pancreatic deoxyribonuclease I and skeletal muscle actin.
AuthorsMannherz HG, Goody RS, Konrad M, Nowak E
JournalEur J Biochem
PubMed ID6244947
The rate of exchange of actin-bound nucleotide is decreased by a factor of about 20 when actin is complexed with DNAase I without affecting the binding constant of calcium for actin. Binding constants of DNAase I to monomeric and filamentous actin were determined to be 5 X 10(8) M-1 and ... More
Conformation change in reconstituted thin filament studied with fluorescence energy transfer between epsilon-ADP bound to F-actin and NBD-Cl bound to troponin-C.
AuthorsMiki M
JournalBiochim Biophys Acta
PubMed ID454674
The interaction of troponin and F-actin was studied by the methods of fluorescence energy transfer. We used epsilon-ADP bound to F-actin as a fluorescence donor and 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole bound to troponin-C in troponin as a fluorescence acceptor. The efficiency of the fluorescence energy transfer of this system was larger in the ... More
ATP and other purine nucleotides stimulate the inactivation of ornithine transcarbamylase by broken lysosomes.
AuthorsNavarro A, Grisolía S
JournalFEBS Lett
PubMed ID6559691
Ornithine transcarbamylase (OTC) is very stable in rat liver homogenates, or mitochondria. However, pure OTC from rat or beef liver is inactivated by broken lysosomes. ATP and a number of purine nucleotides, particularly epsilon-ATP, stimulate the inactivation of OTC by broken lysosomes. Inactivation of OTC by trypsin and elastase is ... More
An active-site-directed adenosine triphosphate analogue binds to the beta-subunits of factor F1 mitochondrial adenosine triphosphatase with its triphosphate moiety.
AuthorsDrutsa VL, Kozlov IA, Milgrom YM, Shabarova ZA, Sokolova NI
JournalBiochem J
PubMed ID159698
The reaction of the mixed anhydride of [3H]ATP and mesitylenecarboxylic acid and soluble mitochondrial adenosine triphosphatase is accompanied by the covalent binding of one molecule of the inhibitor to a molecule of the enzyme and results in the inhibition of adenosine triphosphatase activity by more than 90%. The electrophoresis of ... More
The process in which nucleotide is buried into the active site of heavy meromyosin.
AuthorsAndo T, Duke JA
JournalBiochem Biophys Res Commun
PubMed ID6137220
The process in which nucleotide is buried into the active site of heavy meromyosin was studied with stopped-flow apparatus by monitoring the time-course of the large fluorescence increase of 1,N6-ethenoadenosine triphosphate (epsilon-ATP) when it binds from acrylamide-containing solutions. We have recently reported that free epsilon-ATP fluorescence is effectively quenched by ... More
Presence of epsilon-adenosine tetraphosphate in chromaffin granules after transport of epsilon-ATP.
AuthorsGualix J, Abal M, Pintor J, Miras-Portugal MT
JournalFEBS Lett
PubMed ID8706915
Adenosine 5'-tetraphosphate (Ap4) is a natural constituent of chromaffin granules with concentration values of 2.2 +/- 0.1 nmol/mg of protein and a ratio 245 +/- 40 times lower with respect to ATP (n = 4). The granular transport of epsilon-ATP resulted in a time- and concentration-dependent production of epsilon-adenosine tetraphosphate ... More
Fluorescence energy transfer between heterologous active sites of affinity-labeled aspartokinase of Escherichia coli.
AuthorsWright K, Takahashi M
JournalBiochemistry
PubMed ID192266
The distance between aspartokinase and homoserine dehydrogenase active sites was determined using fluorescence energy transfer between modified substrates. The fluorescent 1,N(6)-ethenoadenosine 5'-triphosphate was bound at the kinase active site by Co(III) affinity labeling. Reduced thionicotinamide adenine dinucleotide phosphate quenched the fluorescence of bound nucleotide. Fluorescence depolarization measurements led to a ... More
Enzymatic synthesis of ATP analogs and their purification by reverse-phase high-performance liquid chromatography.
AuthorsSchobert B
JournalAnal Biochem
PubMed ID7793630
The enzymatic syntheses of ATP analogs, such as tubercidin 5'-triphosphate, formycin A 5'-triphosphate, and etheno-ATP, from their respective mono- and diphosphate are described. The reaction products were purified by reverse-phase HPLC using a C-18 matrix and a volatile mobile phase at pH 7, with tributylamine as the ion-pairing agent. Each ... More
Regulatory effects of purine nucleotide analogs with liver glutamate dehydrogenase.
AuthorsLascu I, Bârzu T, Ty NG, Ngoc LD, Bârzu O, Mantsch HH
JournalBiochim Biophys Acta
PubMed ID18180
A total of 26 different purine nucleotides with specific modifications in the base moiety and/or in the polyphosphate chain as well as various combinations of nucleotides were tested as allosteric effectors of beef liver glutamate dehydrogenase (L-glutamate : NAD(P)+ oxidoreductase (deaminating), EC 1.4.1.3). The capacity of these nucleotide analogs to ... More
Functional Effects of Nemaline Myopathy Mutations on Human Skeletal {alpha}-Actin.
AuthorsMiller BM, Trybus KM,
JournalJ Biol Chem
PubMed ID18477565
Mutations in human alpha-skeletal actin have been implicated in causing congenital nemaline myopathy, a disease characterized histopathologically by nemaline bodies in skeletal muscle and manifested in the patient as skeletal muscle weakness. Here we investigate the functional effects of three severe nemaline myopathy mutations (V43F, A138P, and R183G) in human ... More
Fluorescence energy transfer between points in G-actin: the nucleotide-binding site, the metal-binding site and Cys-373 residue.
AuthorsMiki M, Wahl P
JournalBiochim Biophys Acta
PubMed ID3978110
Fluorescence energy transfers were studied in order to investigate the spatial relationships between the nucleotide-binding site, the metal-binding site and the Cys-373 residue in the G-actin molecule. When 1-N6-ethenoadenosine-5'-triphosphate (epsilon-ATP) in the nucleotide-binding site and Co2+ or Ni2+ in the metal-binding site were used as fluorescence donor and acceptor, respectively, ... More
A catalytic site of protein disulfide isomerase probed with adenosine-5'-triphosphate analogs.
AuthorsChurchich JE, Lee KS
JournalBiochim Biophys Acta
PubMed ID11004547
Anthraniloyl adenosine-5'-triphosphate (Ant-ATP) and etheno-adenosine-5'-triphosphate (epsilon-ATP) complexed to Mg(2+) ions are substrates of protein disulfide isomerase (PDI). epsilon-ATP, coordinated to Tb(3+) ions, was used as a probe of the ATPase binding site. Sensitized luminescence arising from resonance energy transfer from epsilon-adenine to Tb(3+) is quenched by PDI. The luminescence results ... More