EnzChek™ 髓过氧化物酶 (MPO) 活性分析试剂盒
EnzChek™ 髓过氧化物酶 (MPO) 活性分析试剂盒
引用和文献 (8)
Invitrogen™

EnzChek™ 髓过氧化物酶 (MPO) 活性分析试剂盒

髓过氧化物酶 (MPO) 是一种独特的过氧化物酶，除具有过氧化活性外，还能催化过氧化氢 (H2O2) 和氯离子 (Cl-) 发生反应形成次氯酸 (HOCl)。EnzChek™ 髓过氧化物酶了解更多信息
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货号数量
E338561 Kit
货号 E33856
价格（CNY)
12,654.00
Each
添加至购物车
数量:
1 Kit
价格（CNY)
12,654.00
Each
添加至购物车
髓过氧化物酶 (MPO) 是一种独特的过氧化物酶，除具有过氧化活性外，还能催化过氧化氢 (H2O2) 和氯离子 (Cl-) 发生反应形成次氯酸 (HOCl)。EnzChek™ 髓过氧化物酶 (MPO) 活性分析试剂盒可用于测定溶液和细胞裂解物中 MPO 的氯化和过氧化活性。试剂盒提供了无荧光 3'-（p-氨基苯基）荧光素 (APF)，检测氯化反应时，次氯酸盐 (-OCl) 选择性将 APF 裂解，生成荧光产物。检测过氧化反应时，H2O2生成的氧化还原中间体 MPO-I 和 MPO-II 将无荧光 Amplex™ UltraRed 试剂 (A36006) 氧化成荧光产物。EnzChek™ 髓过氧化物酶活性测定试剂盒可以在室温下，在一个较宽的动态范围内（1.5～200 ng/mL）连续检测这些反应的活性。其迅速（30分钟）、灵敏和混合读取方便的特点使得该试剂盒成为测定 MPO（髓过氧化物酶）活性和高通量筛选 MPO 特异性抑制剂等试验的理想之选。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光强度
数量1 Kit
运输条件湿冰
底物属性化学底物
目标酶髓过氧化物酶
适用于（应用）髓过氧化物酶 (MPO) 细胞活力测定试剂盒
产品线EnzChek
产品类型髓过氧物酶 (MPO) 检测
Unit SizeEach
内容与储存
在冰箱（2°C 至 8°C）中避光储存。

常见问题解答 (FAQ)

What is the dynamic range of the EnzChek Myeloperoxidase (MPO) Activity Assay Kit (Cat. No. E33856)?

The EnzChek Myeloperoixdase Activity Assay Kit (Cat. No. E33856) can detect myeloperoxidase activity at a broad dynamic range of 1.5 to 200 ng/mL.

What can interfere with the EnzChek Myeloperoxidase (MPO) Activity assay?

MPO is inhibited by azide, diclofenac, methimazole, quercetin, rutin, and salicylhydroxamic acid. Make certain that samples do not include sodium azide. Endogenous catalases can interfere with the assay; catalase activity may be inhibited by using 3-amino-1,2,4-triazole. Detergents, common components in cell lysis buffers, should be avoided; use freeze/thawing and/or mechanical methods to lyse cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (8)

引用和文献
Abstract
Endothelial CYP epoxygenase overexpression and soluble epoxide hydrolase disruption attenuate acute vascular inflammatory responses in mice.
Authors:Deng Y, Edin ML, Theken KN, Schuck RN, Flake GP, Kannon MA, DeGraff LM, Lih FB, Foley J, Bradbury JA, Graves JP, Tomer KB, Falck JR, Zeldin DC, Lee CR,
Journal:FASEB J
PubMed ID:21059750
Cytochrome P-450 (CYP)-derived epoxyeicosatrienoic acids (EETs) possess potent anti-inflammatory effects in vitro. However, the effect of increased CYP-mediated EET biosynthesis and decreased soluble epoxide hydrolase (sEH, Ephx2)-mediated EET hydrolysis on vascular inflammation in vivo has not been rigorously investigated. Consequently, we characterized acute vascular inflammatory responses to endotoxin in transgenic ... More
G-CSF treatment of severe congenital neutropenia reverses neutropenia but does not correct the underlying functional deficiency of the neutrophil in defending against microorganisms.
Authors:Donini M, Fontana S, Savoldi G, Vermi W, Tassone L, Gentili F, Zenaro E, Ferrari D, Notarangelo LD, Porta F, Facchetti F, Notarangelo LD, Dusi S, Badolato R
Journal:Blood
PubMed ID:17311988
'The treatment of children affected by severe congenital neutropenia (SCN) with G-CSF strongly reduces the risk of sepsis by reversing neutropenia. However, SCN patients who respond to the treatment with the growth factor still have an elevated risk of succumbing to sepsis. Because the disease is usually caused by heterozygous ... More
Targeted deletion of tumor suppressor PTEN augments neutrophil function and enhances host defense in neutropenia-associated pneumonia.
Authors:Li Y, Jia Y, Pichavant M, Loison F, Sarraj B, Kasorn A, You J, Robson BE, Umetsu DT, Mizgerd JP, Ye K, Luo HR,
Journal:Blood
PubMed ID:19286998
'Neutropenia and related infections are the most important dose-limiting toxicities in anticancer chemotherapy and radiotherapy. In this study, we explored a new strategy for augmenting host defense in neutropenia-related pneumonia. Phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P(3)) signaling in neutrophils was elevated by depleting PTEN, a phosphatidylinositol 3''-phosphatase that hydrolyzes PtdIns(3,4,5)P(3). In myeloid-specific PTEN knockout ... More
Inflammation-induced, 3'UTR-dependent translational inhibition of Hsp70 mRNA impairs intestinal homeostasis.
Authors:Hu S, Zhu X, Triggs JR, Tao Y, Wang Y, Lichtenstein L, Bissonnette M, Musch MW, Chang EB,
Journal:Am J Physiol Gastrointest Liver Physiol
PubMed ID:19299581
'Although the inducible heat shock protein 70 (Hsp70) is essential for maintaining intestinal homeostasis in colitis, it is translationally downregulated in inflamed colonic mucosa, paradoxically rendering the gut more susceptible to injury. We examined the basis for this process by analyzing the role of untranslated regions (UTR) of Hsp70 mRNA ... More
Functional consequence of positive selection revealed through rational mutagenesis of human myeloperoxidase.
Authors:Loughran NB, Hinde S, McCormick-Hill S, Leidal KG, Bloomberg S, Loughran ST, O'Connor B, O'Fágáin C, Nauseef WM, O'Connell MJ,
Journal:Mol Biol Evol
PubMed ID:22355012
'Myeloperoxidase (MPO) is a member of the mammalian heme peroxidase (MHP) multigene family. Whereas all MHPs oxidize specific halides to generate the corresponding hypohalous acid, MPO is unique in its capacity to oxidize chloride at physiologic pH to produce hypochlorous acid (HOCl), a potent microbicide that contributes to neutrophil-mediated host ... More
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