EnzChek™ Myeloperoxidase (MPO) Activity Assay Kit

Citations & References (8)

Invitrogen™

EnzChek™ Myeloperoxidase (MPO) Activity Assay Kit

Myeloperoxidase (MPO) is a unique peroxidase that, in addition to its peroxidation activity, also catalyzes the conversion of hydrogen peroxideRead more

Catalog Number	Quantity
E33856	1 Kit

Catalog number E33856

Price (CNY)

12,654.00

Add to cart

Quantity:

1 Kit

Price (CNY)

12,654.00

Add to cart

Myeloperoxidase (MPO) is a unique peroxidase that, in addition to its peroxidation activity, also catalyzes the conversion of hydrogen peroxide (H₂O₂) and chloride (Cl^-) to hypochlorous acid (HOCl). The EnzChek™ Myeloperoxidase (MPO) Activity Assay Kit provides assays for the determination of both chlorination and peroxidation activities of MPO in solution and in cell lysates. For detection of chlorination, the kit includes nonfluorescent 3'-(p-aminophenyl) fluorescein (APF), which is selectively cleaved by hypochlorite (^-OCl) to yield fluorescein. Peroxidation is detected using nonfluorescent Amplex™ UltraRed reagent (A36006), which is oxidized by the H₂O₂-generated redox intermediates MPO-I and MPO-II to form a fluorescent product. The EnzChek™ Myeloperoxidase Activity Assay Kit can be used to continuously detect these activities at room temperature over a broad dynamic range (1.5 to 200 ng/mL). The speed (30 minutes), sensitivity, and mix-and-read convenience make this kit ideal for measuring MPO activities and for high-throughput screening for MPO-specific inhibitors.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection MethodFluorescence Intensity

Quantity1 Kit

Shipping ConditionWet Ice

Substrate PropertiesChemical Substrate

Target EnzymeMyeloperoxidase

For Use With (Application)Myeloperoxidase (MPO) Activity Assay

Product LineEnzChek

Product TypeEnzChek Assay Kit

Unit SizeEach

Contents & Storage

Store in refrigerator 2°C to 8°C and protect from light.

Frequently asked questions (FAQs)

What is the dynamic range of the EnzChek Myeloperoxidase (MPO) Activity Assay Kit (Cat. No. E33856)?

The EnzChek Myeloperoixdase Activity Assay Kit (Cat. No. E33856) can detect myeloperoxidase activity at a broad dynamic range of 1.5 to 200 ng/mL.

What can interfere with the EnzChek Myeloperoxidase (MPO) Activity assay?

MPO is inhibited by azide, diclofenac, methimazole, quercetin, rutin, and salicylhydroxamic acid. Make certain that samples do not include sodium azide. Endogenous catalases can interfere with the assay; catalase activity may be inhibited by using 3-amino-1,2,4-triazole. Detergents, common components in cell lysis buffers, should be avoided; use freeze/thawing and/or mechanical methods to lyse cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (8)

Citations & References

Abstract

Endothelial CYP epoxygenase overexpression and soluble epoxide hydrolase disruption attenuate acute vascular inflammatory responses in mice.

Authors:Deng Y, Edin ML, Theken KN, Schuck RN, Flake GP, Kannon MA, DeGraff LM, Lih FB, Foley J, Bradbury JA, Graves JP, Tomer KB, Falck JR, Zeldin DC, Lee CR,

Journal:FASEB J

PubMed ID:21059750

Cytochrome P-450 (CYP)-derived epoxyeicosatrienoic acids (EETs) possess potent anti-inflammatory effects in vitro. However, the effect of increased CYP-mediated EET biosynthesis and decreased soluble epoxide hydrolase (sEH, Ephx2)-mediated EET hydrolysis on vascular inflammation in vivo has not been rigorously investigated. Consequently, we characterized acute vascular inflammatory responses to endotoxin in transgenic ... More

G-CSF treatment of severe congenital neutropenia reverses neutropenia but does not correct the underlying functional deficiency of the neutrophil in defending against microorganisms.

Authors:Donini M, Fontana S, Savoldi G, Vermi W, Tassone L, Gentili F, Zenaro E, Ferrari D, Notarangelo LD, Porta F, Facchetti F, Notarangelo LD, Dusi S, Badolato R

Journal:Blood

PubMed ID:17311988

'The treatment of children affected by severe congenital neutropenia (SCN) with G-CSF strongly reduces the risk of sepsis by reversing neutropenia. However, SCN patients who respond to the treatment with the growth factor still have an elevated risk of succumbing to sepsis. Because the disease is usually caused by heterozygous ... More

Targeted deletion of tumor suppressor PTEN augments neutrophil function and enhances host defense in neutropenia-associated pneumonia.

Authors:Li Y, Jia Y, Pichavant M, Loison F, Sarraj B, Kasorn A, You J, Robson BE, Umetsu DT, Mizgerd JP, Ye K, Luo HR,

Journal:Blood

PubMed ID:19286998

'Neutropenia and related infections are the most important dose-limiting toxicities in anticancer chemotherapy and radiotherapy. In this study, we explored a new strategy for augmenting host defense in neutropenia-related pneumonia. Phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P(3)) signaling in neutrophils was elevated by depleting PTEN, a phosphatidylinositol 3''-phosphatase that hydrolyzes PtdIns(3,4,5)P(3). In myeloid-specific PTEN knockout ... More

Inflammation-induced, 3'UTR-dependent translational inhibition of Hsp70 mRNA impairs intestinal homeostasis.

Authors:Hu S, Zhu X, Triggs JR, Tao Y, Wang Y, Lichtenstein L, Bissonnette M, Musch MW, Chang EB,

Journal:Am J Physiol Gastrointest Liver Physiol

PubMed ID:19299581

'Although the inducible heat shock protein 70 (Hsp70) is essential for maintaining intestinal homeostasis in colitis, it is translationally downregulated in inflamed colonic mucosa, paradoxically rendering the gut more susceptible to injury. We examined the basis for this process by analyzing the role of untranslated regions (UTR) of Hsp70 mRNA ... More

Functional consequence of positive selection revealed through rational mutagenesis of human myeloperoxidase.

Authors:Loughran NB, Hinde S, McCormick-Hill S, Leidal KG, Bloomberg S, Loughran ST, O'Connor B, O'Fágáin C, Nauseef WM, O'Connell MJ,

Journal:Mol Biol Evol

PubMed ID:22355012

'Myeloperoxidase (MPO) is a member of the mammalian heme peroxidase (MHP) multigene family. Whereas all MHPs oxidize specific halides to generate the corresponding hypohalous acid, MPO is unique in its capacity to oxidize chloride at physiologic pH to produce hypochlorous acid (HOCl), a potent microbicide that contributes to neutrophil-mediated host ... More

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